IGF2R Monoclonal Antibody (2G11) | Invitrogen MA1-066 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

IGF2R Monoclonal Antibody (2G11)

catalog :

MA1-066

quantity :

100 µg

price :

US 425.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2G11

reactivity :

African green monkey, hamsters, human, rat, bovine, domestic rabbit

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

more info or order :

Invitrogen product webpage

citations: 39

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; bovine; 2 ug/ml; loading ...; fig 3a	Deissler H, Lang G, Lang G. Fate of the Fc fusion protein aflibercept in retinal endothelial cells: competition of recycling and degradation. Graefes Arch Clin Exp Ophthalmol. 2019;257:83-94 pubmed publisher
immunocytochemistry; human; loading ...; fig 5d western blot; human; loading ...; fig 5c	Duclos C, Champagne A, Carrier J, Saucier C, Lavoie C, Denault J. Caspase-mediated proteolysis of the sorting nexin 2 disrupts retromer assembly and potentiates Met/hepatocyte growth factor receptor signaling. Cell Death Discov. 2017;3:16100 pubmed publisher
immunohistochemistry; bovine; loading ...; fig 4e	Sulaiman A, Zolnierczyk K, Japa O, Owen J, Maddison B, Emes R, et al. A Trematode Parasite Derived Growth Factor Binds and Exerts Influences on Host Immune Functions via Host Cytokine Receptor Complexes. PLoS Pathog. 2016;12:e1005991 pubmed publisher
immunocytochemistry; human; fig s2	Corcelle Termeau E, Vindeløv S, Hämälistö S, Mograbi B, Keldsbo A, Bräsen J, et al. Excess sphingomyelin disturbs ATG9A trafficking and autophagosome closure. Autophagy. 2016;12:833-49 pubmed publisher
immunocytochemistry; human; 1:100; fig 4	Kahra D, Mondol T, Niemiec M, Wittung Stafshede P. Human Copper Chaperone Atox1 Translocates to the Nucleus but does not Bind DNA In Vitro. Protein Pept Lett. 2015;22:532-8 pubmed
immunocytochemistry; human; fig 3	Nonnenmacher M, Cintrat J, Gillet D, Weber T. Syntaxin 5-dependent retrograde transport to the trans-Golgi network is required for adeno-associated virus transduction. J Virol. 2015;89:1673-87 pubmed publisher
immunocytochemistry; human; fig s1	Bakthavatsalam D, Soung R, Tweardy D, Chiu W, Dixon R, Woodside D. Chaperonin-containing TCP-1 complex directly binds to the cytoplasmic domain of the LOX-1 receptor. FEBS Lett. 2014;588:2133-40 pubmed publisher
immunocytochemistry; human; 1:400	Ishikawa K, Nara A, Matsumoto K, Hanafusa H. EGFR-dependent phosphorylation of leucine-rich repeat kinase LRRK1 is important for proper endosomal trafficking of EGFR. Mol Biol Cell. 2012;23:1294-306 pubmed publisher
immunocytochemistry; human; 1:400	Humphries W, Szymanski C, Payne C. Endo-lysosomal vesicles positive for Rab7 and LAMP1 are terminal vesicles for the transport of dextran. PLoS ONE. 2011;6:e26626 pubmed publisher
immunocytochemistry; African green monkey; 1:400	Humphries W, Fay N, Payne C. Intracellular degradation of low-density lipoprotein probed with two-color fluorescence microscopy. Integr Biol (Camb). 2010;2:536-44 pubmed publisher
immunocytochemistry; hamsters; fig 6	Mire C, White J, Whitt M. A spatio-temporal analysis of matrix protein and nucleocapsid trafficking during vesicular stomatitis virus uncoating. PLoS Pathog. 2010;6:e1000994 pubmed publisher
immunocytochemistry; human; fig 4	Jacobs D, Weigert R, Grode K, Donaldson J, Cheney R. Myosin Vc is a molecular motor that functions in secretory granule trafficking. Mol Biol Cell. 2009;20:4471-88 pubmed publisher
immunocytochemistry; human	Naslavsky N, McKenzie J, Altan Bonnet N, Sheff D, Caplan S. EHD3 regulates early-endosome-to-Golgi transport and preserves Golgi morphology. J Cell Sci. 2009;122:389-400 pubmed publisher
immunocytochemistry; human western blot; human	Ganley I, Espinosa E, Pfeffer S. A syntaxin 10-SNARE complex distinguishes two distinct transport routes from endosomes to the trans-Golgi in human cells. J Cell Biol. 2008;180:159-72 pubmed publisher
western blot; domestic rabbit; 1:4	Mettler S, Ghayouri S, Christensen G, Forte J. Modulatory role of phosphoinositide 3-kinase in gastric acid secretion. Am J Physiol Gastrointest Liver Physiol. 2007;293:G532-43 pubmed
immunocytochemistry; human; 1:25	Doody A, Putnam D. Identification of compartments involved in mammalian subcellular trafficking pathways by indirect immunofluorescence. Methods Mol Med. 2006;127:127-36 pubmed
immunocytochemistry; human western blot; human	Ganley I, Pfeffer S. Cholesterol accumulation sequesters Rab9 and disrupts late endosome function in NPC1-deficient cells. J Biol Chem. 2006;281:17890-9 pubmed
immunocytochemistry; human	Tanabe K, Torii T, Natsume W, Braesch Andersen S, Watanabe T, Satake M. A novel GTPase-activating protein for ARF6 directly interacts with clathrin and regulates clathrin-dependent endocytosis. Mol Biol Cell. 2005;16:1617-28 pubmed
	Shi M, Chen B, Mahajan D, Boh B, Zhou Y, Dutta B, et al. Amino acids stimulate the endosome-to-Golgi trafficking through Ragulator and small GTPase Arl5. Nat Commun. 2018;9:4987 pubmed publisher
	Simonetti B, Danson C, Heesom K, Cullen P. Sequence-dependent cargo recognition by SNX-BARs mediates retromer-independent transport of CI-MPR. J Cell Biol. 2017;216:3695-3712 pubmed publisher
	Girard M, Poupon V, Blondeau F, McPherson P. The DnaJ-domain protein RME-8 functions in endosomal trafficking. J Biol Chem. 2005;280:40135-43 pubmed
	Choudhury R, Diao A, Zhang F, Eisenberg E, Saint Pol A, Williams C, et al. Lowe syndrome protein OCRL1 interacts with clathrin and regulates protein trafficking between endosomes and the trans-Golgi network. Mol Biol Cell. 2005;16:3467-79 pubmed
	Mazzone M, Baldassarre M, Beznoussenko G, Giacchetti G, Cao J, Zucker S, et al. Intracellular processing and activation of membrane type 1 matrix metalloprotease depends on its partitioning into lipid domains. J Cell Sci. 2004;117:6275-87 pubmed
	Ganley I, Carroll K, Bittova L, Pfeffer S. Rab9 GTPase regulates late endosome size and requires effector interaction for its stability. Mol Biol Cell. 2004;15:5420-30 pubmed
	Kokkonen N, Rivinoja A, Kauppila A, Suokas M, Kellokumpu I, Kellokumpu S. Defective acidification of intracellular organelles results in aberrant secretion of cathepsin D in cancer cells. J Biol Chem. 2004;279:39982-8 pubmed
	Matsushita M, Tanaka S, Nakamura N, Inoue H, Kanazawa H. A novel kinesin-like protein, KIF1Bbeta3 is involved in the movement of lysosomes to the cell periphery in non-neuronal cells. Traffic. 2004;5:140-51 pubmed
	Sawaguchi A, McDonald K, Forte J. High-pressure freezing of isolated gastric glands provides new insight into the fine structure and subcellular localization of H+/K+-ATPase in gastric parietal cells. J Histochem Cytochem. 2004;52:77-86 pubmed
	Yoshino A, Bieler B, Harper D, Cowan D, Sutterwala S, Gay D, et al. A role for GRIP domain proteins and/or their ligands in structure and function of the trans Golgi network. J Cell Sci. 2003;116:4441-54 pubmed
	Polishchuk E, Di Pentima A, Luini A, Polishchuk R. Mechanism of constitutive export from the golgi: bulk flow via the formation, protrusion, and en bloc cleavage of large trans-golgi network tubular domains. Mol Biol Cell. 2003;14:4470-85 pubmed
	Umeda A, Fujita H, Kuronita T, Hirosako K, Himeno M, Tanaka Y. Distribution and trafficking of MPR300 is normal in cells with cholesterol accumulated in late endocytic compartments: evidence for early endosome-to-TGN trafficking of MPR300. J Lipid Res. 2003;44:1821-32 pubmed
	Hall A, Krishnamoorthy L, Orlow S. Accumulation of tyrosinase in the endolysosomal compartment is induced by U18666A. Pigment Cell Res. 2003;16:149-58 pubmed
	Rodriguez O, Cheney R. Human myosin-Vc is a novel class V myosin expressed in epithelial cells. J Cell Sci. 2002;115:991-1004 pubmed
	Shinotsuka C, Yoshida Y, Kawamoto K, Takatsu H, Nakayama K. Overexpression of an ADP-ribosylation factor-guanine nucleotide exchange factor, BIG2, uncouples brefeldin A-induced adaptor protein-1 coat dissociation and membrane tubulation. J Biol Chem. 2002;277:9468-73 pubmed
	Ammar D, Nguyen P, Forte J. Functionally distinct pools of actin in secretory cells. Am J Physiol Cell Physiol. 2001;281:C407-17 pubmed
	Puertollano R, Aguilar R, Gorshkova I, Crouch R, Bonifacino J. Sorting of mannose 6-phosphate receptors mediated by the GGAs. Science. 2001;292:1712-6 pubmed
	Duman J, Tyagarajan K, Kolsi M, Moore H, Forte J. Expression of rab11a N124I in gastric parietal cells inhibits stimulatory recruitment of the H+-K+-ATPase. Am J Physiol. 1999;277:C361-72 pubmed publisher
	Chow D, Forte J. Characterization of the beta-subunit of the H(+)-K(+)-ATPase using an inhibitory monoclonal antibody. Am J Physiol. 1993;265:C1562-70 pubmed
	Dintzis S, Velculescu V, Pfeffer S. Receptor extracellular domains may contain trafficking information. Studies of the 300-kDa mannose 6-phosphate receptor. J Biol Chem. 1994;269:12159-66 pubmed
	MacDonald R, Tepper M, Clairmont K, Perregaux S, Czech M. Serum form of the rat insulin-like growth factor II/mannose 6-phosphate receptor is truncated in the carboxyl-terminal domain. J Biol Chem. 1989;264:3256-61 pubmed

product information

Product Type :

Antibody

Product Name :

IGF2R Monoclonal Antibody (2G11)

Catalog # :

MA1-066

Quantity :

100 µg

Price :

US 425.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Bovine, Human, Non-human primate, Rat

Applications :

Flow Cytometry: 1 µg / 10^6 cells, Immunocytochemistry: 2-5 µg/mL, Immunohistochemistry (PFA fixed): Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: 1 µg/mL

Species :

Bovine, Human, Non-human primate, Rat

Clone :

2G11

Isotype :

IgG2a

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

Lysosomal enzymes containing one or two mannose 6-phosphate (man6P) moieties are moved about in the cell by two distinct but interconnected cycles by means of approximately 300 kDa cation independent mannose 6-phospate receptors (CI-MPR). The biosynthetic cycle refers to the MPR transport of newly synthesized lysosomal enzymes from the trans Golgi network to late endosomes or early lysosomes. The endocytic cycle transports extracellular lysosomal enzymes from the plasma membrane via clathrin-coated vesicles to early endosomes. The entire pool of MPRs cycles between these cellular compartments every 3 hours. The steady state distribution of MPR's is predominantly within late endosomes, fewer in the trans Golgi network and approximately 10 % at cell surface. In addition to its man6P binding activity, the MPR contains a separate binding site for the type II insulin-like growth factor and is capable of binding both man6P and IGF-II simultaneously. An approximately 240 kDa soluble, truncated form, representing the extracellular domain of the protein, has also been found circulating in serum and is capable of binding both ligands.

Immunogen :

Purified bovine ~300 kDa CI-MPR.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 1 µg / 10^6 cells, Immunocytochemistry: 2-5 µg/mL, Immunohistochemistry (PFA fixed): Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: 1 µg/mL

Aliases :

300 kDa mannose 6-phosphate receptor; AI661837; cation-independent mannose 6-phosphate receptor; cation-independent mannose 6-phosphate receptor protein; cation-independent mannose-6-phosphate receptor; cation-independent MPR; CD222; CI Man-6-P receptor; CI-M6PR; CIMPR; CI-MPR; Igf2r; IGF-II receptor; IGF-II/CI-MPR; Igfr2; insulin like growth factor 2 receptor; insulin-like growth factor 2 receptor; Insulin-like growth factor II receptor; insulin-like growth factor receptor type 2; M6P; M6P/IGF2 receptor; M6P/IGF2R; M6PR; M6P-R; mannose-6-phosphate receptor, cation independent; mannose-6-phosphate/insulin-like growth factor II receptor; MPR 300; MPR1; MPR300; MPRI; similar to mannose-6-phosphate/insulin-like growth factor II receptor

more info or order :

Invitrogen product webpage