Clathrin Heavy Chain Monoclonal Antibody (X22) | Invitrogen MA1-065 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Clathrin Heavy Chain Monoclonal Antibody (X22)

catalog :

MA1-065

quantity :

100 µL

price :

US 399.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

X22

reactivity :

hamsters, African green monkey, Chinese hamsters, human, mouse, rat, bovine, pigs

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, blocking or activating experiments

more info or order :

Invitrogen product webpage

citations: 107

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; loading ...; fig 5c western blot; human; loading ...; fig s6a	Almeida Souza L, Frank R, García Nafría J, Colussi A, Gunawardana N, Johnson C, et al. A Flat BAR Protein Promotes Actin Polymerization at the Base of Clathrin-Coated Pits. Cell. 2018;174:325-337.e14 pubmed publisher
immunocytochemistry; mouse; 1:250; loading ...; fig 6i	Krey J, Dumont R, Wilmarth P, David L, Johnson K, Barr Gillespie P. ELMOD1 Stimulates ARF6-GTP Hydrolysis to Stabilize Apical Structures in Developing Vestibular Hair Cells. J Neurosci. 2018;38:843-857 pubmed publisher
immunocytochemistry; pigs ; loading ...; fig 5d western blot; pigs ; loading ...; fig 5a immunocytochemistry; human; fig 2b western blot; human; fig 1b	Poulsen E, Iannuzzi F, Rasmussen H, Maier T, Enghild J, Jørgensen A, et al. An Aberrant Phosphorylation of Amyloid Precursor Protein Tyrosine Regulates Its Trafficking and the Binding to the Clathrin Endocytic Complex in Neural Stem Cells of Alzheimer's Disease Patients. Front Mol Neurosci. 2017;10:59 pubmed publisher
western blot; human; 1:500; fig 2b	Chiang C, Flint M, Lin J, Spiropoulou C. Endocytic Pathways Used by Andes Virus to Enter Primary Human Lung Endothelial Cells. PLoS ONE. 2016;11:e0164768 pubmed publisher
immunocytochemistry; human; fig s12	Yi J, Manna A, Barr V, Hong J, Neuman K, Samelson L. madSTORM: a superresolution technique for large-scale multiplexing at single-molecule accuracy. Mol Biol Cell. 2016;27:3591-3600 pubmed
immunocytochemistry; human; fig 3	Fu Y, Winter P, Rojas R, Wang V, McAuliffe M, Patterson G. Axial superresolution via multiangle TIRF microscopy with sequential imaging and photobleaching. Proc Natl Acad Sci U S A. 2016;113:4368-73 pubmed publisher
immunocytochemistry; human; 1:2000; tbl 4	Wollscheid H, Biancospino M, He F, Magistrati E, Molteni E, Lupia M, et al. Diverse functions of myosin VI elucidated by an isoform-specific Î±-helix domain. Nat Struct Mol Biol. 2016;23:300-308 pubmed publisher
western blot; human; 1:10,000; fig 3	Zhao L, Marciano A, Rivet C, Imperiale M. Caveolin- and clathrin-independent entry of BKPyV into primary human proximal tubule epithelial cells. Virology. 2016;492:66-72 pubmed publisher
immunocytochemistry; rat; fig 4	Wang C, Wang Y, Hu M, Chai Z, Wu Q, Huang R, et al. Synaptotagmin-11 inhibits clathrin-mediated and bulk endocytosis. EMBO Rep. 2016;17:47-63 pubmed publisher
immunocytochemistry; human	Hayashi A, Asanuma D, Kamiya M, Urano Y, Okabe S. High affinity receptor labeling based on basic leucine zipper domain peptides conjugated with pH-sensitive fluorescent dye: Visualization of AMPA-type glutamate receptor endocytosis in living neurons. Neuropharmacology. 2016;100:66-75 pubmed publisher
immunocytochemistry; human; 1:500; fig 6 western blot; human; 1:2000; fig 5	Zhao Z, Sagare A, Ma Q, Halliday M, Kong P, Kisler K, et al. Central role for PICALM in amyloid-Î² blood-brain barrier transcytosis and clearance. Nat Neurosci. 2015;18:978-87 pubmed publisher
immunocytochemistry; human; fig s4j immunocytochemistry; African green monkey; fig s2d western blot; African green monkey; fig s2e	Boucrot E, Ferreira A, Almeida Souza L, Debard S, Vallis Y, Howard G, et al. Endophilin marks and controls a clathrin-independent endocytic pathway. Nature. 2015;517:460-5 pubmed publisher
immunocytochemistry; human; fig 3	Matsuda M, Suzuki R, Kataoka C, Watashi K, Aizaki H, Kato N, et al. Alternative endocytosis pathway for productive entry of hepatitis C virus. J Gen Virol. 2014;95:2658-67 pubmed publisher
immunohistochemistry; rat	Nakano Kobayashi A, Tai Y, Nadif Kasri N, Van Aelst L. The X-linked mental retardation protein OPHN1 interacts with Homer1b/c to control spine endocytic zone positioning and expression of synaptic potentiation. J Neurosci. 2014;34:8665-71 pubmed publisher
immunocytochemistry; human; 1:100	Olofsson A, Nygård Skalman L, Obi I, Lundmark R, Arnqvist A. Uptake of Helicobacter pylori vesicles is facilitated by clathrin-dependent and clathrin-independent endocytic pathways. MBio. 2014;5:e00979-14 pubmed publisher
western blot; human; 1:2000	Takahashi Y, Fujii T, Fujita K, Shimizu T, Higuchi T, Tabuchi Y, et al. Functional coupling of chloride-proton exchanger ClC-5 to gastric H+,K+-ATPase. Biol Open. 2014;3:12-21 pubmed publisher
immunocytochemistry; human western blot; human	Méndez E, Muñoz Yañez C, Sánchez San Martín C, Aguirre Crespo G, Baños Lara M, Gutierrez M, et al. Characterization of human astrovirus cell entry. J Virol. 2014;88:2452-60 pubmed publisher
immunocytochemistry; human	Sorrentino V, Nelson J, Maspero E, Marques A, Scheer L, Polo S, et al. The LXR-IDOL axis defines a clathrin-, caveolae-, and dynamin-independent endocytic route for LDLR internalization and lysosomal degradation. J Lipid Res. 2013;54:2174-84 pubmed publisher
immunocytochemistry; human	Ylivinkka I, Hu Y, Chen P, Rantanen V, Hautaniemi S, Nyman T, et al. Netrin-1-induced activation of Notch signaling mediates glioblastoma cell invasion. J Cell Sci. 2013;126:2459-69 pubmed publisher
immunocytochemistry; mouse	Yamada H, Abe T, Satoh A, Okazaki N, Tago S, Kobayashi K, et al. Stabilization of actin bundles by a dynamin 1/cortactin ring complex is necessary for growth cone filopodia. J Neurosci. 2013;33:4514-26 pubmed publisher
immunocytochemistry; human	Varsano T, Taupin V, Guo L, Baterina O, Farquhar M. The PDZ protein GIPC regulates trafficking of the LPA1 receptor from APPL signaling endosomes and attenuates the cell's response to LPA. PLoS ONE. 2012;7:e49227 pubmed publisher
western blot; human; 1:500	Harmon B, Schudel B, Maar D, Kozina C, Ikegami T, Tseng C, et al. Rift Valley fever virus strain MP-12 enters mammalian host cells via caveola-mediated endocytosis. J Virol. 2012;86:12954-70 pubmed publisher
western blot; rat; 1:1000	Rong Y, Liu M, Ma L, Du W, Zhang H, Tian Y, et al. Clathrin and phosphatidylinositol-4,5-bisphosphate regulate autophagic lysosome reformation. Nat Cell Biol. 2012;14:924-34 pubmed publisher
western blot; human	Prabhu Y, Burgos P, Schindler C, Farías G, Magadán J, Bonifacino J. Adaptor protein 2-mediated endocytosis of the ?-secretase BACE1 is dispensable for amyloid precursor protein processing. Mol Biol Cell. 2012;23:2339-51 pubmed publisher
blocking or activating experiments; mouse; 240 uM	Yao L, Rao Y, Varga K, Wang C, Xiao P, Lindau M, et al. Synaptotagmin 1 is necessary for the Ca2+ dependence of clathrin-mediated endocytosis. J Neurosci. 2012;32:3778-85 pubmed publisher
western blot; pigs	Gupta S, Yan Y, Malhotra D, Liu J, Xie Z, Najjar S, et al. Ouabain and insulin induce sodium pump endocytosis in renal epithelium. Hypertension. 2012;59:665-72 pubmed publisher
immunocytochemistry; African green monkey	Lampe A, Haucke V, Sigrist S, Heilemann M, Schmoranzer J. Multi-colour direct STORM with red emitting carbocyanines. Biol Cell. 2012;104:229-37 pubmed publisher
western blot; mouse	Abdel Shakor A, Atia M, Kwiatkowska K, Sobota A. Cell surface ceramide controls translocation of transferrin receptor to clathrin-coated pits. Cell Signal. 2012;24:677-84 pubmed publisher
immunocytochemistry; mouse	Shakor A, Taniguchi M, Kitatani K, Hashimoto M, Asano S, Hayashi A, et al. Sphingomyelin synthase 1-generated sphingomyelin plays an important role in transferrin trafficking and cell proliferation. J Biol Chem. 2011;286:36053-62 pubmed publisher
immunocytochemistry; human	von Kleist L, Stahlschmidt W, Bulut H, Gromova K, Puchkov D, Robertson M, et al. Role of the clathrin terminal domain in regulating coated pit dynamics revealed by small molecule inhibition. Cell. 2011;146:471-84 pubmed publisher
immunocytochemistry; African green monkey	Jones S, Shim S, He J, Zhuang X. Fast, three-dimensional super-resolution imaging of live cells. Nat Methods. 2011;8:499-508 pubmed publisher
immunocytochemistry; human; 1:500	Doyon J, Zeitler B, Cheng J, Cheng A, Cherone J, Santiago Y, et al. Rapid and efficient clathrin-mediated endocytosis revealed in genome-edited mammalian cells. Nat Cell Biol. 2011;13:331-7 pubmed publisher
immunohistochemistry; human	Contreras Ruiz L, de la Fuente M, Párraga J, López García A, Fernandez I, Seijo B, et al. Intracellular trafficking of hyaluronic acid-chitosan oligomer-based nanoparticles in cultured human ocular surface cells. Mol Vis. 2011;17:279-90 pubmed
immunocytochemistry; rat	Govindarajan R, Chakraborty S, Johnson K, Falk M, Wheelock M, Johnson K, et al. Assembly of connexin43 into gap junctions is regulated differentially by E-cadherin and N-cadherin in rat liver epithelial cells. Mol Biol Cell. 2010;21:4089-107 pubmed publisher
immunocytochemistry; mouse; 1:250 western blot; mouse; 1:500	Uthaiah R, Hudspeth A. Molecular anatomy of the hair cell's ribbon synapse. J Neurosci. 2010;30:12387-99 pubmed publisher
immunohistochemistry; mouse	Wu M, Huang B, Graham M, Raimondi A, Heuser J, Zhuang X, et al. Coupling between clathrin-dependent endocytic budding and F-BAR-dependent tubulation in a cell-free system. Nat Cell Biol. 2010;12:902-8 pubmed publisher
immunocytochemistry; human; 1:1000	Yoo S, Shin J, Park S. EphA8-ephrinA5 signaling and clathrin-mediated endocytosis is regulated by Tiam-1, a Rac-specific guanine nucleotide exchange factor. Mol Cells. 2010;29:603-9 pubmed publisher
immunocytochemistry; human	Bhattacharyya S, Warfield K, Ruthel G, Bavari S, Aman M, Hope T. Ebola virus uses clathrin-mediated endocytosis as an entry pathway. Virology. 2010;401:18-28 pubmed publisher
immunocytochemistry; human	Kanerva K, Mäkitie L, Bäck N, Andersson L. Ornithine decarboxylase antizyme inhibitor 2 regulates intracellular vesicle trafficking. Exp Cell Res. 2010;316:1896-906 pubmed publisher
immunocytochemistry; human; 1:500	Bai T, Seebald J, Kim K, Ding H, Szeto D, Chang H. Disruption of zebrafish cyclin G-associated kinase (GAK) function impairs the expression of Notch-dependent genes during neurogenesis and causes defects in neuronal development. BMC Dev Biol. 2010;10:7 pubmed publisher
immunocytochemistry; human western blot; human	Thiery J, Keefe D, Saffarian S, Martinvalet D, Walch M, Boucrot E, et al. Perforin activates clathrin- and dynamin-dependent endocytosis, which is required for plasma membrane repair and delivery of granzyme B for granzyme-mediated apoptosis. Blood. 2010;115:1582-93 pubmed publisher
immunohistochemistry; rat	Tao Cheng J, Dosemeci A, Gallant P, Miller S, Galbraith J, Winters C, et al. Rapid turnover of spinules at synaptic terminals. Neuroscience. 2009;160:42-50 pubmed publisher
immunocytochemistry; mouse	Pons V, Luyet P, Morel E, Abrami L, van der Goot F, Parton R, et al. Hrs and SNX3 functions in sorting and membrane invagination within multivesicular bodies. PLoS Biol. 2008;6:e214 pubmed publisher
immunocytochemistry; mouse	Braun V, Deschamps C, Raposo G, Benaroch P, Benmerah A, Chavrier P, et al. AP-1 and ARF1 control endosomal dynamics at sites of FcR mediated phagocytosis. Mol Biol Cell. 2007;18:4921-31 pubmed
immunocytochemistry; human; fig 3	Zanardi A, Giorgetti L, Botrugno O, Minucci S, Milani P, Pelicci P, et al. Immunocell-array for molecular dissection of multiple signaling pathways in mammalian cells. Mol Cell Proteomics. 2007;6:939-47 pubmed
immunocytochemistry; human	Hara T, Honda K, Shitashige M, Ono M, Matsuyama H, Naito K, et al. Mass spectrometry analysis of the native protein complex containing actinin-4 in prostate cancer cells. Mol Cell Proteomics. 2007;6:479-91 pubmed
immunocytochemistry; rat	Yamanushi T, Shui Z, Leach R, Dobrzynski H, Claydon T, Boyett M. Role of internalization of M2 muscarinic receptor via clathrin-coated vesicles in desensitization of the muscarinic K+ current in heart. Am J Physiol Heart Circ Physiol. 2007;292:H1737-46 pubmed
immunohistochemistry; human	Kim S, Choi H, Lee K, Hong N, Sung B, Choi K, et al. Interaction of SPIN90 with syndapin is implicated in clathrin-mediated endocytic pathway in fibroblasts. Genes Cells. 2006;11:1197-211 pubmed
immunocytochemistry; human	Royle S, Lagnado L. Trimerisation is important for the function of clathrin at the mitotic spindle. J Cell Sci. 2006;119:4071-8 pubmed
immunocytochemistry; rat; 1:500	Zhu P, Soderblom C, Tao Cheng J, Stadler J, Blackstone C. SPG3A protein atlastin-1 is enriched in growth cones and promotes axon elongation during neuronal development. Hum Mol Genet. 2006;15:1343-53 pubmed
immunohistochemistry; human	Tosoni D, Puri C, Confalonieri S, Salcini A, De Camilli P, Tacchetti C, et al. TTP specifically regulates the internalization of the transferrin receptor. Cell. 2005;123:875-88 pubmed
immunocytochemistry; human; 1:100	O Donnell V, LaRocco M, Duque H, Baxt B. Analysis of foot-and-mouth disease virus internalization events in cultured cells. J Virol. 2005;79:8506-18 pubmed
immunocytochemistry; hamsters	Yim Y, Scarselletta S, Zang F, Wu X, Lee D, Kang Y, et al. Exchange of clathrin, AP2 and epsin on clathrin-coated pits in permeabilized tissue culture cells. J Cell Sci. 2005;118:2405-13 pubmed
immunocytochemistry; human	Royle S, Bright N, Lagnado L. Clathrin is required for the function of the mitotic spindle. Nature. 2005;434:1152-7 pubmed
immunocytochemistry; mouse	Sigismund S, Woelk T, Puri C, Maspero E, Tacchetti C, Transidico P, et al. Clathrin-independent endocytosis of ubiquitinated cargos. Proc Natl Acad Sci U S A. 2005;102:2760-5 pubmed
immunocytochemistry; human	Tanabe K, Torii T, Natsume W, Braesch Andersen S, Watanabe T, Satake M. A novel GTPase-activating protein for ARF6 directly interacts with clathrin and regulates clathrin-dependent endocytosis. Mol Biol Cell. 2005;16:1617-28 pubmed
immunocytochemistry; rat	Ihara Y, Manabe S, Kanda M, Kawano H, Nakayama T, Sekine I, et al. Increased expression of protein C-mannosylation in the aortic vessels of diabetic Zucker rats. Glycobiology. 2005;15:383-92 pubmed
	Aki S, Yoshioka K, Takuwa N, Takuwa Y. TGFβ receptor endocytosis and Smad signaling require synaptojanin1, PI3K-C2α-, and INPP4B-mediated phosphoinositide conversions. Mol Biol Cell. 2020;31:360-372 pubmed publisher
	Dirck A, Whyte M, Hudson A. HHV-7 U21 exploits Golgi quality control carriers to reroute class I MHC molecules to lysosomes. Mol Biol Cell. 2020;31:196-208 pubmed publisher
	Scheefhals N, Catsburg L, Westerveld M, Blanpied T, Hoogenraad C, MacGillavry H. Shank Proteins Couple the Endocytic Zone to the Postsynaptic Density to Control Trafficking and Signaling of Metabotropic Glutamate Receptor 5. Cell Rep. 2019;29:258-269.e8 pubmed publisher
	Wallroth A, Koch P, Marat A, Krause E, Haucke V. Protein kinase N controls a lysosomal lipid switch to facilitate nutrient signalling via mTORC1. Nat Cell Biol. 2019;21:1093-1101 pubmed publisher
	Tsygankova O, Keen J. A unique role for clathrin light chain A in cell spreading and migration. J Cell Sci. 2019;132: pubmed publisher
	Ford C, NANS A, Boucrot E, Hayward R. Chlamydia exploits filopodial capture and a macropinocytosis-like pathway for host cell entry. PLoS Pathog. 2018;14:e1007051 pubmed publisher
	Zlatic S, Vrailas Mortimer A, Gokhale A, Carey L, Scott E, Burch R, et al. Rare Disease Mechanisms Identified by Genealogical Proteomics of Copper Homeostasis Mutant Pedigrees. Cell Syst. 2018;6:368-380.e6 pubmed publisher
	de Castro Martín I, Fournier G, Sachse M, Pizarro Cerda J, Risco C, Naffakh N. Influenza virus genome reaches the plasma membrane via a modified endoplasmic reticulum and Rab11-dependent vesicles. Nat Commun. 2017;8:1396 pubmed publisher
	Soriano Castell D, Chavero A, Rentero C, Bosch M, Vidal Quadras M, Pol A, et al. ROCK1 is a novel Rac1 effector to regulate tubular endocytic membrane formation during clathrin-independent endocytosis. Sci Rep. 2017;7:6866 pubmed publisher
	Bugge M, Bergstrom B, Eide O, Solli H, Kjønstad I, Stenvik J, et al. Surface Toll-like receptor 3 expression in metastatic intestinal epithelial cells induces inflammatory cytokine production and promotes invasiveness. J Biol Chem. 2017;292:15408-15425 pubmed publisher
	Leyton Puig D, Isogai T, Argenzio E, van den Broek B, Klarenbeek J, Janssen H, et al. Flat clathrin lattices are dynamic actin-controlled hubs for clathrin-mediated endocytosis and signalling of specific receptors. Nat Commun. 2017;8:16068 pubmed publisher
	Zhao W, Hanson L, Lou H, Akamatsu M, Chowdary P, Santoro F, et al. Nanoscale manipulation of membrane curvature for probing endocytosis in live cells. Nat Nanotechnol. 2017;12:750-756 pubmed publisher
	Tolvanen T, Dash S, Polianskyte Prause Z, Dumont V, Lehtonen S. Lack of CD2AP disrupts Glut4 trafficking and attenuates glucose uptake in podocytes. J Cell Sci. 2015;128:4588-600 pubmed publisher
	Ciraolo E, Iezzi M, Marone R, Marengo S, Curcio C, Costa C, et al. Phosphoinositide 3-kinase p110beta activity: key role in metabolism and mammary gland cancer but not development. Sci Signal. 2008;1:ra3 pubmed publisher
	Li J, Peters P, Bai M, Dai J, Bos E, Kirchhausen T, et al. An ACAP1-containing clathrin coat complex for endocytic recycling. J Cell Biol. 2007;178:453-64 pubmed
	Nishi K, Saigo K. Cellular internalization of green fluorescent protein fused with herpes simplex virus protein VP22 via a lipid raft-mediated endocytic pathway independent of caveolae and Rho family GTPases but dependent on dynamin and Arf6. J Biol Chem. 2007;282:27503-17 pubmed
	Radulescu A, Siddhanta A, Shields D. A role for clathrin in reassembly of the Golgi apparatus. Mol Biol Cell. 2007;18:94-105 pubmed
	Zhao X, Claude A, Chun J, Shields D, Presley J, Melancon P. GBF1, a cis-Golgi and VTCs-localized ARF-GEF, is implicated in ER-to-Golgi protein traffic. J Cell Sci. 2006;119:3743-53 pubmed
	Lampugnani M, Orsenigo F, Gagliani M, Tacchetti C, Dejana E. Vascular endothelial cadherin controls VEGFR-2 internalization and signaling from intracellular compartments. J Cell Biol. 2006;174:593-604 pubmed
	Grewal P, Boton M, Ramirez K, Collins B, Saito A, Green R, et al. ST6Gal-I restrains CD22-dependent antigen receptor endocytosis and Shp-1 recruitment in normal and pathogenic immune signaling. Mol Cell Biol. 2006;26:4970-81 pubmed
	Khan E, Heidinger J, Levy M, Lisanti M, Ravid T, Goldkorn T. Epidermal growth factor receptor exposed to oxidative stress undergoes Src- and caveolin-1-dependent perinuclear trafficking. J Biol Chem. 2006;281:14486-93 pubmed
	Chen H, De Camilli P. The association of epsin with ubiquitinated cargo along the endocytic pathway is negatively regulated by its interaction with clathrin. Proc Natl Acad Sci U S A. 2005;102:2766-71 pubmed
	Moskowitz H, Yokoyama C, Ryan T. Highly cooperative control of endocytosis by clathrin. Mol Biol Cell. 2005;16:1769-76 pubmed
	Zhang L, Gjoerup O, Roberts T. The serine/threonine kinase cyclin G-associated kinase regulates epidermal growth factor receptor signaling. Proc Natl Acad Sci U S A. 2004;101:10296-301 pubmed
	Carreno S, Engqvist Goldstein A, Zhang C, McDonald K, Drubin D. Actin dynamics coupled to clathrin-coated vesicle formation at the trans-Golgi network. J Cell Biol. 2004;165:781-8 pubmed
	Hale M, Marvaud J, Popoff M, Stiles B. Detergent-resistant membrane microdomains facilitate Ib oligomer formation and biological activity of Clostridium perfringens iota-toxin. Infect Immun. 2004;72:2186-93 pubmed
	Engqvist Goldstein A, Zhang C, Carreno S, Barroso C, Heuser J, Drubin D. RNAi-mediated Hip1R silencing results in stable association between the endocytic machinery and the actin assembly machinery. Mol Biol Cell. 2004;15:1666-79 pubmed
	Liang W, Curran P, Hoang Q, Moreland R, Fishman P. Differences in endosomal targeting of human (beta)1- and (beta)2-adrenergic receptors following clathrin-mediated endocytosis. J Cell Sci. 2004;117:723-34 pubmed
	Moskowitz H, Heuser J, McGraw T, Ryan T. Targeted chemical disruption of clathrin function in living cells. Mol Biol Cell. 2003;14:4437-47 pubmed
	Ivanov A, Nusrat A, Parkos C. Endocytosis of epithelial apical junctional proteins by a clathrin-mediated pathway into a unique storage compartment. Mol Biol Cell. 2004;15:176-88 pubmed
	Elenko E, Fischer T, Niesman I, Harding T, McQuistan T, von Zastrow M, et al. Spatial regulation of Galphai protein signaling in clathrin-coated membrane microdomains containing GAIP. Mol Pharmacol. 2003;64:11-20 pubmed
	Carniti C, Perego C, Mondellini P, Pierotti M, Bongarzone I. PP1 inhibitor induces degradation of RETMEN2A and RETMEN2B oncoproteins through proteosomal targeting. Cancer Res. 2003;63:2234-43 pubmed
	Artalejo C, Elhamdani A, Palfrey H. Sustained stimulation shifts the mechanism of endocytosis from dynamin-1-dependent rapid endocytosis to clathrin- and dynamin-2-mediated slow endocytosis in chromaffin cells. Proc Natl Acad Sci U S A. 2002;99:6358-63 pubmed
	Engqvist Goldstein A, Warren R, Kessels M, Keen J, Heuser J, Drubin D. The actin-binding protein Hip1R associates with clathrin during early stages of endocytosis and promotes clathrin assembly in vitro. J Cell Biol. 2001;154:1209-23 pubmed
	Metzler M, Legendre Guillemin V, Gan L, Chopra V, Kwok A, McPherson P, et al. HIP1 functions in clathrin-mediated endocytosis through binding to clathrin and adaptor protein 2. J Biol Chem. 2001;276:39271-6 pubmed
	Vecchi M, Polo S, Poupon V, Van de Loo J, Benmerah A, Di Fiore P. Nucleocytoplasmic shuttling of endocytic proteins. J Cell Biol. 2001;153:1511-7 pubmed
	Suzuki J, Ohnsihi H, Shibata H, Wada A, Hirayama T, Iiri T, et al. Dynamin is involved in human epithelial cell vacuolation caused by the Helicobacter pylori-produced cytotoxin VacA. J Clin Invest. 2001;107:363-70 pubmed
	Slepnev V, Ochoa G, Butler M, De Camilli P. Tandem arrangement of the clathrin and AP-2 binding domains in amphiphysin 1 and disruption of clathrin coat function by amphiphysin fragments comprising these sites. J Biol Chem. 2000;275:17583-9 pubmed
	Jiang R, Gao B, Prasad K, Greene L, Eisenberg E. Hsc70 chaperones clathrin and primes it to interact with vesicle membranes. J Biol Chem. 2000;275:8439-47 pubmed
	Greener T, Zhao X, Nojima H, Eisenberg E, Greene L. Role of cyclin G-associated kinase in uncoating clathrin-coated vesicles from non-neuronal cells. J Biol Chem. 2000;275:1365-70 pubmed
	Engqvist Goldstein A, Kessels M, Chopra V, Hayden M, Drubin D. An actin-binding protein of the Sla2/Huntingtin interacting protein 1 family is a novel component of clathrin-coated pits and vesicles. J Cell Biol. 1999;147:1503-18 pubmed
	Nordeng T, Bakke O. Overexpression of proteins containing tyrosine- or leucine-based sorting signals affects transferrin receptor trafficking. J Biol Chem. 1999;274:21139-48 pubmed
	Fischer T, Elenko E, McCaffery J, DeVries L, Farquhar M. Clathrin-coated vesicles bearing GAIP possess GTPase-activating protein activity in vitro. Proc Natl Acad Sci U S A. 1999;96:6722-7 pubmed
	Simpson F, Peden A, Christopoulou L, Robinson M. Characterization of the adaptor-related protein complex, AP-3. J Cell Biol. 1997;137:835-45 pubmed
	Chang M, Mallet W, Mostov K, Brodsky F. Adaptor self-aggregation, adaptor-receptor recognition and binding of alpha-adaptin subunits to the plasma membrane contribute to recruitment of adaptor (AP2) components of clathrin-coated pits. EMBO J. 1993;12:2169-80 pubmed
	Seaman M, Ball C, Robinson M. Targeting and mistargeting of plasma membrane adaptors in vitro. J Cell Biol. 1993;123:1093-105 pubmed
	Brodsky F. Clathrin structure characterized with monoclonal antibodies. I. Analysis of multiple antigenic sites. J Cell Biol. 1985;101:2047-54 pubmed
	Wong D, Brodsky F. 100-kD proteins of Golgi- and trans-Golgi network-associated coated vesicles have related but distinct membrane binding properties. J Cell Biol. 1992;117:1171-9 pubmed
	Nathke I, Heuser J, Lupas A, Stock J, Turck C, Brodsky F. Folding and trimerization of clathrin subunits at the triskelion hub. Cell. 1992;68:899-910 pubmed
	Mäkelä A, Mäkinen E, Lorenz K, Rikalainen H. [Changes in the cervical spine in juvenile rheumatoid arthritis (author's transl)]. Rofo. 1979;131:420-7 pubmed

product information

Product Type :

Antibody

Product Name :

Clathrin Heavy Chain Monoclonal Antibody (X22)

Catalog # :

MA1-065

Quantity :

100 µL

Price :

US 399.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Bovine, Hamster, Human, Mouse, Non-human primate, Rat

Applications :

Flow Cytometry: 1/200, Immunocytochemistry: Assay-dependent, Immunohistochemistry: 1:100, Immunomicroscopy: Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: 1:100-1:500

Species :

Bovine, Hamster, Human, Mouse, Non-human primate, Rat

Clone :

X22

Isotype :

IgG1

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network.

Immunogen :

Purified human brain clathrin heavy chain.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 1/200, Immunocytochemistry: Assay-dependent, Immunohistochemistry: 1:100, Immunomicroscopy: Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: 1:100-1:500

Aliases :

3110065L21Rik; CHC; CHC17; CHC22; clathrin heavy chain; clathrin heavy chain 1; Clathrin heavy chain 2; clathrin heavy chain like 1; clathrin heavy chain on chromosome 17; Clathrin heavy chain on chromosome 22; clathrin, heavy chain (Hc); clathrin, heavy chain-like 1; clathrin, heavy polypeptide (Hc); Clathrin, heavy polypeptide D; clathrin, heavy polypeptide-like 1; clathrin, heavy polypeptide-like 2; CLH17; CLH-17; CLH22; CLH-22; Cltc; CLTCL; CLTCL1; CLTCL2; CLTD; Hc; KIAA0034; R74732

more info or order :

Invitrogen product webpage