alpha Adaptin Monoclonal Antibody (AP6) | Invitrogen MA1-064 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

alpha Adaptin Monoclonal Antibody (AP6)

catalog :

MA1-064

quantity :

100 µL

price :

US 399.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AP6

reactivity :

Chinese hamsters, hamsters, African green monkey, human, mouse, rat, bovine, pigs

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

more info or order :

Invitrogen product webpage

citations: 48

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; loading ...; fig 3d	Elkhatib N, Bresteau E, Baschieri F, Rioja A, van Niel G, Vassilopoulos S, et al. Tubular clathrin/AP-2 lattices pinch collagen fibers to support 3D cell migration. Science. 2017;356: pubmed publisher
immunocytochemistry; human; fig 2b western blot; human; fig 1b immunocytochemistry; pigs ; loading ...; fig 5d western blot; pigs ; loading ...; fig 5a	Poulsen E, Iannuzzi F, Rasmussen H, Maier T, Enghild J, Jørgensen A, et al. An Aberrant Phosphorylation of Amyloid Precursor Protein Tyrosine Regulates Its Trafficking and the Binding to the Clathrin Endocytic Complex in Neural Stem Cells of Alzheimer's Disease Patients. Front Mol Neurosci. 2017;10:59 pubmed publisher
immunocytochemistry; human; loading ...; fig 2d	Chien J, Tsen S, Chien C, Liu H, Tung C, Lin C. ?TAT1 downregulation induces mitotic catastrophe in HeLa and A549 cells. Cell Death Discov. 2016;2:16006 pubmed publisher
immunocytochemistry; human; fig s5	De Franceschi N, Arjonen A, Elkhatib N, Denessiouk K, Wrobel A, Wilson T, et al. Selective integrin endocytosis is driven by interactions between the integrin Î±-chain and AP2. Nat Struct Mol Biol. 2016;23:172-9 pubmed publisher
immunocytochemistry; rat; fig 4	Wang C, Wang Y, Hu M, Chai Z, Wu Q, Huang R, et al. Synaptotagmin-11 inhibits clathrin-mediated and bulk endocytosis. EMBO Rep. 2016;17:47-63 pubmed publisher
immunocytochemistry; African green monkey; fig s2d western blot; human; fig s1f	Boucrot E, Ferreira A, Almeida Souza L, Debard S, Vallis Y, Howard G, et al. Endophilin marks and controls a clathrin-independent endocytic pathway. Nature. 2015;517:460-5 pubmed publisher
immunocytochemistry; human	Vehlow A, Soong D, Vizcay Barrena G, Bodo C, Law A, Perera U, et al. Endophilin, Lamellipodin, and Mena cooperate to regulate F-actin-dependent EGF-receptor endocytosis. EMBO J. 2013;32:2722-34 pubmed publisher
immunocytochemistry; mouse; fig 2	Park R, Shen H, Liu L, Liu X, Ferguson S, De Camilli P. Dynamin triple knockout cells reveal off target effects of commonly used dynamin inhibitors. J Cell Sci. 2013;126:5305-12 pubmed publisher
western blot; human	Cihil K, Ellinger P, Fellows A, Stolz D, Madden D, Swiatecka Urban A. Disabled-2 protein facilitates assembly polypeptide-2-independent recruitment of cystic fibrosis transmembrane conductance regulator to endocytic vesicles in polarized human airway epithelial cells. J Biol Chem. 2012;287:15087-99 pubmed publisher
immunocytochemistry; human	Morén B, Shah C, Howes M, Schieber N, McMahon H, Parton R, et al. EHD2 regulates caveolar dynamics via ATP-driven targeting and oligomerization. Mol Biol Cell. 2012;23:1316-29 pubmed publisher
immunocytochemistry; human	Cole N, Donaldson J. Releasable SNAP-tag probes for studying endocytosis and recycling. ACS Chem Biol. 2012;7:464-9 pubmed publisher
immunocytochemistry; human	Roberts R, Peden A, Buss F, Bright N, Latouche M, Reilly M, et al. Mistargeting of SH3TC2 away from the recycling endosome causes Charcot-Marie-Tooth disease type 4C. Hum Mol Genet. 2010;19:1009-18 pubmed publisher
immunocytochemistry; human western blot; human	Puri C, Chibalina M, Arden S, Kruppa A, Kendrick Jones J, Buss F. Overexpression of myosin VI in prostate cancer cells enhances PSA and VEGF secretion, but has no effect on endocytosis. Oncogene. 2010;29:188-200 pubmed publisher
immunocytochemistry; human	Schmidt M, Maritzen T, Kukhtina V, Higman V, Doglio L, Barak N, et al. Regulation of endosomal membrane traffic by a Gadkin/AP-1/kinesin KIF5 complex. Proc Natl Acad Sci U S A. 2009;106:15344-9 pubmed publisher
immunocytochemistry; human; 25 ug/ml	Hood F, Royle S. Functional equivalence of the clathrin heavy chains CHC17 and CHC22 in endocytosis and mitosis. J Cell Sci. 2009;122:2185-90 pubmed publisher
western blot; mouse; 1:1000	Ameen N, Apodaca G. Defective CFTR apical endocytosis and enterocyte brush border in myosin VI-deficient mice. Traffic. 2007;8:998-1006 pubmed
immunocytochemistry; human; fig 3	Zanardi A, Giorgetti L, Botrugno O, Minucci S, Milani P, Pelicci P, et al. Immunocell-array for molecular dissection of multiple signaling pathways in mammalian cells. Mol Cell Proteomics. 2007;6:939-47 pubmed
immunoprecipitation; mouse immunocytochemistry; mouse	Corallini S, Fera S, Grisanti L, Falciatori I, Muciaccia B, Stefanini M, et al. Expression of the adaptor protein m-Numb in mouse male germ cells. Reproduction. 2006;132:887-97 pubmed
immunohistochemistry; African green monkey; 1:200 immunohistochemistry; human; 1:200	Piehl M, Lehmann C, Gumpert A, Denizot J, Segretain D, Falk M. Internalization of large double-membrane intercellular vesicles by a clathrin-dependent endocytic process. Mol Biol Cell. 2007;18:337-47 pubmed
immunocytochemistry; rat; 1:500	Zhu P, Soderblom C, Tao Cheng J, Stadler J, Blackstone C. SPG3A protein atlastin-1 is enriched in growth cones and promotes axon elongation during neuronal development. Hum Mol Genet. 2006;15:1343-53 pubmed
immunocytochemistry; human	Katoh Y, Imakagura H, Futatsumori M, Nakayama K. Recruitment of clathrin onto endosomes by the Tom1-Tollip complex. Biochem Biophys Res Commun. 2006;341:143-9 pubmed
immunocytochemistry; hamsters	Yim Y, Scarselletta S, Zang F, Wu X, Lee D, Kang Y, et al. Exchange of clathrin, AP2 and epsin on clathrin-coated pits in permeabilized tissue culture cells. J Cell Sci. 2005;118:2405-13 pubmed
	Yotsuya M, Bertagna A, Hasan N, Bicknell S, Sato T, Reed D. Neuron/Glial Antigen 2-Type VI Collagen Interactions During Murine Temporomandibular Joint Osteoarthritis. Sci Rep. 2019;9:56 pubmed publisher
	Shi L, Hines T, Bergson C, Smith D. Coupling of microtubule motors with AP-3 generated organelles in axons by NEEP21 family member calcyon. Mol Biol Cell. 2018;29:2055-2068 pubmed publisher
	Wijasa T, Sylvester M, Brocke Ahmadinejad N, Kummer M, Brosseron F, Gieselmann V, et al. Proteome profiling of s-nitrosylated synaptosomal proteins by isobaric mass tags. J Neurosci Methods. 2017;291:95-100 pubmed publisher
	Stoneham C, Singh R, Jia X, Xiong Y, Guatelli J. Endocytic activity of HIV-1 Vpu: Phosphoserine-dependent interactions with clathrin adaptors. Traffic. 2017;18:545-561 pubmed publisher
	Park B, Yim Y, Zhao X, Olszewski M, Eisenberg E, Greene L. The clathrin-binding and J-domains of GAK support the uncoating and chaperoning of clathrin by Hsc70 in the brain. J Cell Sci. 2015;128:3811-21 pubmed publisher
	Austin C, Lawrence D, Peden A, Varfolomeev E, Totpal K, De MaziÃ¨re A, et al. Death-receptor activation halts clathrin-dependent endocytosis. Proc Natl Acad Sci U S A. 2006;103:10283-10288 pubmed publisher
	Grati M, Schneider M, Lipkow K, Strehler E, Wenthold R, Kachar B. Rapid turnover of stereocilia membrane proteins: evidence from the trafficking and mobility of plasma membrane Ca(2+)-ATPase 2. J Neurosci. 2006;26:6386-95 pubmed
	Girard M, Poupon V, Blondeau F, McPherson P. The DnaJ-domain protein RME-8 functions in endosomal trafficking. J Biol Chem. 2005;280:40135-43 pubmed
	Janvier K, Bonifacino J. Role of the endocytic machinery in the sorting of lysosome-associated membrane proteins. Mol Biol Cell. 2005;16:4231-42 pubmed
	Moskowitz H, Yokoyama C, Ryan T. Highly cooperative control of endocytosis by clathrin. Mol Biol Cell. 2005;16:1769-76 pubmed
	Wang Y, Keogh R, Hunter M, Mitchell C, Frey R, Javaid K, et al. SHIP2 is recruited to the cell membrane upon macrophage colony-stimulating factor (M-CSF) stimulation and regulates M-CSF-induced signaling. J Immunol. 2004;173:6820-30 pubmed
	Kinlough C, Poland P, Bruns J, Harkleroad K, Hughey R. MUC1 membrane trafficking is modulated by multiple interactions. J Biol Chem. 2004;279:53071-7 pubmed
	Pagano A, Crottet P, Prescianotto Baschong C, Spiess M. In vitro formation of recycling vesicles from endosomes requires adaptor protein-1/clathrin and is regulated by rab4 and the connector rabaptin-5. Mol Biol Cell. 2004;15:4990-5000 pubmed
	Engqvist Goldstein A, Zhang C, Carreno S, Barroso C, Heuser J, Drubin D. RNAi-mediated Hip1R silencing results in stable association between the endocytic machinery and the actin assembly machinery. Mol Biol Cell. 2004;15:1666-79 pubmed
	Nagai M, Meerloo T, Takeda T, Farquhar M. The adaptor protein ARH escorts megalin to and through endosomes. Mol Biol Cell. 2003;14:4984-96 pubmed
	Merrifield C, Feldman M, Wan L, Almers W. Imaging actin and dynamin recruitment during invagination of single clathrin-coated pits. Nat Cell Biol. 2002;4:691-8 pubmed
	Lundmark R, Carlsson S. The beta-appendages of the four adaptor-protein (AP) complexes: structure and binding properties, and identification of sorting nexin 9 as an accessory protein to AP-2. Biochem J. 2002;362:597-607 pubmed
	Engqvist Goldstein A, Warren R, Kessels M, Keen J, Heuser J, Drubin D. The actin-binding protein Hip1R associates with clathrin during early stages of endocytosis and promotes clathrin assembly in vitro. J Cell Biol. 2001;154:1209-23 pubmed
	Vecchi M, Polo S, Poupon V, Van de Loo J, Benmerah A, Di Fiore P. Nucleocytoplasmic shuttling of endocytic proteins. J Cell Biol. 2001;153:1511-7 pubmed
	Reymond A, Meroni G, Fantozzi A, Merla G, Cairo S, Luzi L, et al. The tripartite motif family identifies cell compartments. EMBO J. 2001;20:2140-51 pubmed
	Slepnev V, Ochoa G, Butler M, De Camilli P. Tandem arrangement of the clathrin and AP-2 binding domains in amphiphysin 1 and disruption of clathrin coat function by amphiphysin fragments comprising these sites. J Biol Chem. 2000;275:17583-9 pubmed
	Ooi C, Dell Angelica E, Bonifacino J. ADP-Ribosylation factor 1 (ARF1) regulates recruitment of the AP-3 adaptor complex to membranes. J Cell Biol. 1998;142:391-402 pubmed
	Chang M, Mallet W, Mostov K, Brodsky F. Adaptor self-aggregation, adaptor-receptor recognition and binding of alpha-adaptin subunits to the plasma membrane contribute to recruitment of adaptor (AP2) components of clathrin-coated pits. EMBO J. 1993;12:2169-80 pubmed
	Chin D, Straubinger R, Acton S, Nathke I, Brodsky F. 100-kDa polypeptides in peripheral clathrin-coated vesicles are required for receptor-mediated endocytosis. Proc Natl Acad Sci U S A. 1989;86:9289-93 pubmed
	Wong D, Brodsky F. 100-kD proteins of Golgi- and trans-Golgi network-associated coated vesicles have related but distinct membrane binding properties. J Cell Biol. 1992;117:1171-9 pubmed
	Beck K, Chang M, Brodsky F, Keen J. Clathrin assembly protein AP-2 induces aggregation of membrane vesicles: a possible role for AP-2 in endosome formation. J Cell Biol. 1992;119:787-96 pubmed

image

image 1 :

Immunofluorescent analysis of alpha Adaptin using alpha Adaptin Monoclonal Antibody (AP6) (Product# MA1-064 ) shows staining in MCF-7 Cells. alpha Adaptin (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing alpha Adaptin (Product# MA1-064 ) at a dilution of 1:20 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.

image 2 :

Immunofluorescent analysis of alpha Adaptin using alpha Adaptin Monoclonal Antibody (AP6) (Product# MA1-064 ) shows staining in U251 Cells. alpha Adaptin (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing alpha Adaptin (Product# MA1-064 ) at a dilution of 1:20 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.

image 3 :

Immunofluorescent analysis of alpha Adaptin using alpha Adaptin Monoclonal Antibody (AP6) (Product# MA1-064 ) shows staining in Hela Cells. alpha Adaptin (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing alpha Adaptin (Product# MA1-064 ) at a dilution of 1:20 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.

product information

Product Type :

Antibody

Product Name :

alpha Adaptin Monoclonal Antibody (AP6)

Catalog # :

MA1-064

Quantity :

100 µL

Price :

US 399.00

Clonality :

Monoclonal

Purity :

purified

Host :

Mouse

Reactivity :

Bovine, Hamster, Human, Mouse, Non-human primate, Rat

Applications :

Flow Cytometry: 1 µg / 10^6 cells, Immunocytochemistry: 1-2 µg/mL, Immunohistochemistry (PFA fixed): Assay-dependent, Immunoprecipitation: Assay-dependent, Inhibition Assays: Assay-dependent, Western Blot: 1:200

Species :

Bovine, Hamster, Human, Mouse, Non-human primate, Rat

Clone :

AP6

Isotype :

IgG1

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif.

Immunogen :

Purified adaptors.

Format :

Liquid

Applications w/Dilutions :

Aliases :

100 kDa coated vesicle protein A; adapter-related protein complex 2 alpha-1 subunit; adapter-related protein complex 2 subunit alpha-1; adaptin, alpha A; adaptor protein complex AP-2 subunit alpha-1; adaptor protein complex AP-2, alpha 1 subunit; adaptor related protein complex 2 alpha 1 subunit; adaptor related protein complex 2 subunit alpha 1; adaptor related protein complex 2, alpha 1 subunit; adaptor-related protein complex 2 subunit alpha-1; adaptor-related protein complex 2, alpha 1 subunit; adaptor-related protein complex 2, short form; adaptor-related protein complex AP-2, alpha 1 subunit; Adtaa; ADTAB; alpha1-adaptin; alpha2-adaptin; Alpha-adaptin A; alpha-adaptin C; AP2; AP-2 complex subunit alpha-1; Ap2a1; AP2-ALPHA; CLAPA1; CLAPA2; Clathrin assembly protein complex 2 alpha-A large chain; clathrin-associated/assembly/adaptor protein, large, alpha 1; HIP9; HIP-9; HYPJ; plasma membrane adaptor HA2/AP2 adaptin alpha A subunit

more info or order :

Invitrogen product webpage