Adiponectin Monoclonal Antibody (19F1) | Invitrogen MA1-054 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Adiponectin Monoclonal Antibody (19F1)

catalog :

MA1-054

quantity :

100 µg

price :

US 441.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

19F1

reactivity :

human, mouse, domestic rabbit

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 18

Published Application/Species/Sample/Dilution	Reference
western blot; domestic rabbit; fig 3	Cawthorn W, Scheller E, Parlee S, Pham H, Learman B, Redshaw C, et al. Expansion of Bone Marrow Adipose Tissue During Caloric Restriction Is Associated With Increased Circulating Glucocorticoids and Not With Hypoleptinemia. Endocrinology. 2016;157:508-21 pubmed publisher
western blot; human; 1:1000; fig 1	Davis G, Stephens J, Nelson A. Effect of 12 Weeks of Periodized Resistance Training Upon Total Plasma Adiponectin Concentration in Healthy Young Men. J Strength Cond Res. 2015;29:3097-104 pubmed publisher
western blot; mouse	Fuller S, Richard A, Ribnicky D, Beyl R, Mynatt R, Stephens J. St. John's Wort Has Metabolically Favorable Effects on Adipocytes In Vivo. Evid Based Complement Alternat Med. 2014;2014:862575 pubmed publisher
western blot; human	Richard A, Amini Vaughan Z, Ribnicky D, Stephens J. Naringenin inhibits adipogenesis and reduces insulin sensitivity and adiponectin expression in adipocytes. Evid Based Complement Alternat Med. 2013;2013:549750 pubmed publisher
western blot; mouse	Du B, Cawthorn W, Su A, Doucette C, Yao Y, Hemati N, et al. The transcription factor paired-related homeobox 1 (Prrx1) inhibits adipogenesis by activating transforming growth factor-? (TGF?) signaling. J Biol Chem. 2013;288:3036-47 pubmed publisher
western blot; human; 0.2 ng/ml	Vidal C, Gunaratnam K, Tong J, Duque G. Biochemical changes induced by strontium ranelate in differentiating adipocytes. Biochimie. 2013;95:793-8 pubmed publisher
immunohistochemistry; mouse; 1:1000	Seese R, Babayan A, Katz A, Cox C, Lauterborn J, Lynch G, et al. LTP induction translocates cortactin at distant synapses in wild-type but not Fmr1 knock-out mice. J Neurosci. 2012;32:7403-13 pubmed publisher
western blot; mouse	Park Y, Lee Y, Kim S, Lee E, Kim K, Williams D, et al. Snail, a transcriptional regulator, represses adiponectin expression by directly binding to an E-box motif in the promoter. Metabolism. 2012;61:1622-32 pubmed publisher
western blot; human	Boccara F, Auclair M, Cohen A, Lefèvre C, Prot M, Bastard J, et al. HIV protease inhibitors activate the adipocyte renin angiotensin system. Antivir Ther. 2010;15:363-75 pubmed publisher
western blot; mouse	Vernochet C, Davis K, Scherer P, Farmer S. Mechanisms regulating repression of haptoglobin production by peroxisome proliferator-activated receptor-gamma ligands in adipocytes. Endocrinology. 2010;151:586-94 pubmed publisher
	Wang T, Hill R, Dzieciatkowska M, Zhu L, Infante A, Hu G, et al. Site-Dependent Lineage Preference of Adipose Stem Cells. Front Cell Dev Biol. 2020;8:237 pubmed publisher
	Jorgensen A, Varkey M, Gorkun A, Clouse C, Xu L, Chou Z, et al. Bioprinted Skin Recapitulates Normal Collagen Remodeling in Full-Thickness Wounds. Tissue Eng Part A. 2020;26:512-526 pubmed publisher
	El Ouarrat D, Isaac R, Lee Y, Oh D, Wollam J, Lackey D, et al. TAZ Is a Negative Regulator of PPARγ Activity in Adipocytes and TAZ Deletion Improves Insulin Sensitivity and Glucose Tolerance. Cell Metab. 2020;31:162-173.e5 pubmed publisher
	Seo J, Riopel M, Cabrales P, Huh J, Bandyopadhyay G, Andreyev A, et al. Knockdown of Ant2 Reduces Adipocyte Hypoxia And Improves Insulin Resistance in Obesity. Nat Metab. 2019;1:86-97 pubmed publisher
	Rotter D, Peiris H, Grinsfelder D, Martin A, Burchfield J, Parra V, et al. Regulator of Calcineurin 1 helps coordinate whole-body metabolism and thermogenesis. EMBO Rep. 2018;19: pubmed publisher
	Chan C, Gulati G, Sinha R, Tompkins J, Lopez M, Carter A, et al. Identification of the Human Skeletal Stem Cell. Cell. 2018;175:43-56.e21 pubmed publisher
	Gulati G, Bennett M, Abulaban K, Song H, Zhang X, Ma Q, et al. Prospective validation of a novel renal activity index of lupus nephritis. Lupus. 2017;26:927-936 pubmed publisher
	Cawthorn W, Scheller E, Learman B, Parlee S, Simon B, Mori H, et al. Bone marrow adipose tissue is an endocrine organ that contributes to increased circulating adiponectin during caloric restriction. Cell Metab. 2014;20:368-375 pubmed publisher

image

image 1 :

Immunohistochemistry was performed on biopsies of deparaffinized Human skin tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing Adiponectin (MA1-054) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

image 2 :

product information

Product Type :

Antibody

Product Name :

Adiponectin Monoclonal Antibody (19F1)

Catalog # :

MA1-054

Quantity :

100 µg

Price :

US 441.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Human, Mouse, Rabbit

Applications :

ELISA: Assay-dependent, Immunocytochemistry: 2 µg/mL, Immunohistochemistry (Paraffin): Assay-dependent, Western Blot: 2 µg/mL

Species :

Human, Mouse, Rabbit

Clone :

19F1

Isotype :

IgG

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

Adiponectin is a protein hormone secreted exclusively by adipocytes that regulates the metabolism of lipids and glucose. Adiponectin has been shown to play a role in various physiological processes including homeostasis and obesity. Decreased levels of adiponectin are associated with obese individuals as well as insulin resistance and hyperinsulinemia (type 2 diabetes). In mice adiponectin has been shown to improve insulin signaling, thus ameliorating insulin resistance. Through activation of AMP kinase and PPAR-alpha, adiponectin can increase fatty acid transport, oxidation, and dissipation in skeletal muscle, leading to a reduction of intramyocellular lipid levels and thus improved insulin signaling.

Immunogen :

Full length, recombinant, human adiponectin.

Format :

Liquid

Applications w/Dilutions :

ELISA: Assay-dependent, Immunocytochemistry: 2 µg/mL, Immunohistochemistry (Paraffin): Assay-dependent, Western Blot: 2 µg/mL

Aliases :

30 kDa adipocyte complement-related protein; 30kDa; Acdc; Acrp30; Ad; adipo; adipocyte complement related protein; adipocyte complement related protein of 30 kDa; adipocyte complement-related 30 kDa protein; adipocyte complement-related protein of 30 kDa; adipocyte, C1Q and collagen domain containing; adipocyte, C1q and collagen domain-containing protein; Adipocyte-specific protein AdipoQ; adiponectin; adiponectin, C1Q and collagen domain containing; Adipoq; Adipose most abundant gene transcript 1 protein; adipose specific collagen-like factor; ADIPQTL1; ADPIOQ; ADPN; Apm1; APM-1; APN; GBP28; Gelatin-binding protein; gelatin-binding protein 28

more info or order :

Invitrogen product webpage