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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
TAMRA Monoclonal Antibody (5G5)
catalog :
MA1-041
quantity :
100 µg
price :
US 500.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
5G5
reactivity :
mouse
application :
western blot, immunoprecipitation
more info or order :
citations: 9
| Published Application/Species/Sample/Dilution | Reference |
|---|---|
| |
Okerberg E, Wu J, Zhang B, Samii B, Blackford K, Winn D, et al. High-resolution functional proteomics by active-site peptide profiling. Proc Natl Acad Sci U S A. 2005;102:4996-5001 pubmed
|
image
image 1 :
Immunoprecipitation was performed on purified TAMRA-BSA. The antigen:antibody complex was formed by binding 5µg protein with 10µg of mouse monoclonal antibody recognizing TAMRA (Product # MA1-041) or Mouse IgG, whole molecule ( Product # 31202) overnight on a rocking platform at 4°C. The immune-complex was then captured on 50µl Protein A/G Plus Agarose (Product #20423) . Captured immune-complexes were then washed extensively and proteins eluted with 5X Reducing Sample Loading Dye (Product # 39000). Samples were then resolved on a 4-20% Tris-HCl polyacrylamide gel. Proteins were imaged using Typhoon 9410 Imager at 532nM , Normal Sensitivity.
image 2 :
Western blot analysis was performed by loading various amounts of purified TAMRA-BSA protein onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were then probed with a mouse monoclonal antibody recognizing TAMRA (Product #MA1-041) at a dilution of 1:1000 for 1hour at room temperature on a rocking platform. Membranes were then washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody (Product #31430) at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection was performed using Pierce Super Signal West Pico (Product #34087).
product information
Product Type :
Antibody
Product Name :
TAMRA Monoclonal Antibody (5G5)
Catalog # :
MA1-041
Quantity :
100 µg
Price :
US 500.00
Clonality :
Monoclonal
Purity :
Protein G
Host :
Mouse
Reactivity :
Chemical
Applications :
Immunoprecipitation: 10 µg, Western Blot: 1:1,000
Species :
Chemical
Clone :
5G5
Isotype :
IgG1
Storage :
-20°C
Description :
The red-fluorescent tetramethylrhodamine (TAMRA) azide can be used as a reactive-azido group (Staudinger reagents) that facilitates multiplex labeling when used with phosphine- or alkyne-derivatized tags. In addition, TAMRA fluorophore has been used to label and detect active serine hydrolases in samples by fluorescent gel imaging, capillary electrophoresis or mass spectrometry.
Immunogen :
TAMRA molecule
Format :
Liquid
Applications w/Dilutions :
Immunoprecipitation: 10 µg, Western Blot: 1:1,000
Aliases :
TAMRA
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com81 Wyman Street
Waltham, MA USA 02451
800-678-5599
headquarters: USA