NFATC2 Monoclonal Antibody (25A10.D6.D2) | Invitrogen MA1-025 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

NFATC2 Monoclonal Antibody (25A10.D6.D2)

catalog :

MA1-025

quantity :

100 ug

price :

US 491.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

25A10.D6.D2

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, EMSA, chromatin immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 38

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:1000; loading ...; fig 5a	Cao P, Aoki Y, Badri L, Walker N, Manning C, Lagstein A, et al. Autocrine lysophosphatidic acid signaling activates ?-catenin and promotes lung allograft fibrosis. J Clin Invest. 2017;127:1517-1530 pubmed publisher
immunocytochemistry; mouse; 1:500; fig 5A	Rampoldi F, Brunk F, Bonrouhi M, Federico G, Krunic D, Porubsky S, et al. Deficiency of N-myristoylation reveals calcineurin activity as regulator of IFN-?-producing ?? T cells. J Leukoc Biol. 2017;101:1005-1014 pubmed publisher
western blot; mouse; 1:1000	Zanotti S, Smerdel Ramoya A, Canalis E. Nuclear factor of activated T-cells (NFAT)C2 inhibits Notch receptor signaling in osteoblasts. J Biol Chem. 2013;288:624-32 pubmed publisher
chromatin immunoprecipitation; human	Gaudineau B, Fougere M, Guaddachi F, Lemoine F, de la Grange P, Jauliac S. Lipocalin 2, the TNF-like receptor TWEAKR and its ligand TWEAK act downstream of NFAT1 to regulate breast cancer cell invasion. J Cell Sci. 2012;125:4475-86 pubmed publisher
chromatin immunoprecipitation; mouse western blot; mouse	Li Q, Shakya A, Guo X, Zhang H, Tantin D, Jensen P, et al. Constitutive nuclear localization of NFAT in Foxp3+ regulatory T cells independent of calcineurin activity. J Immunol. 2012;188:4268-77 pubmed publisher
western blot; mouse; 1:1000	Fric J, Lim C, Koh E, Hofmann B, Chen J, Tay H, et al. Calcineurin/NFAT signalling inhibits myeloid haematopoiesis. EMBO Mol Med. 2012;4:269-82 pubmed publisher
flow cytometry; mouse	Huang Y, Sojka D, Fowell D. Cutting edge: Regulatory T cells selectively attenuate, not terminate, T cell signaling by disrupting NF-?B nuclear accumulation in CD4 T cells. J Immunol. 2012;188:947-51 pubmed publisher
EMSA; human	Wang Q, Zhou Y, Weiss H, Chow C, Evers B. NFATc1 regulation of TRAIL expression in human intestinal cells. PLoS ONE. 2011;6:e19882 pubmed publisher
immunoprecipitation; mouse	Ohoka Y, Yokota A, Takeuchi H, Maeda N, Iwata M. Retinoic acid-induced CCR9 expression requires transient TCR stimulation and cooperativity between NFATc2 and the retinoic acid receptor/retinoid X receptor complex. J Immunol. 2011;186:733-44 pubmed publisher
immunocytochemistry; mouse; 2 ug/ml	Arimilli S, Sharma S, Yammani R, Reid S, Parks G, Alexander Miller M. Pivotal Advance: Nonfunctional lung effectors exhibit decreased calcium mobilization associated with reduced expression of ORAI1. J Leukoc Biol. 2010;87:977-88 pubmed publisher
western blot; rat	De Arcangelis V, Coletti D, Canato M, Molinaro M, Adamo S, Reggiani C, et al. Hypertrophy and transcriptional regulation induced in myogenic cell line L6-C5 by an increase of extracellular calcium. J Cell Physiol. 2005;202:787-95 pubmed
	Lee M, Kwon S. Enhanced cytotoxic activity of natural killer cells from increased calcium influx induced by electrical stimulation. PLoS ONE. 2024;19:e0302406 pubmed publisher
	Hao J, Liu C, Gu Z, Yang X, Lan X, Guo X. Dysregulation of Wnt/β-catenin signaling contributes to intestinal inflammation through regulation of group 3 innate lymphoid cells. Nat Commun. 2024;15:2820 pubmed publisher
	D xf6 lz M, Hasiuk M, Gagnon J, Kornete M, Marone R, Bantug G, et al. Forced expression of the non-coding RNA miR-17∼92 restores activation and function in CD28-deficient CD4+ T cells. iScience. 2022;25:105372 pubmed publisher
	Tandl D, Sponagel T, Alansary D, Fuck S, Smit T, Hehlgans S, et al. X-ray irradiation triggers immune response in human T-lymphocytes via store-operated Ca2+ entry and NFAT activation. J Gen Physiol. 2022;154: pubmed publisher
	Liu H, Sun Q, Chen S, Chen L, Jia W, Zhao J, et al. DYRK1A activates NFATC1 to increase glioblastoma migration. Cancer Med. 2021;: pubmed publisher
	Xian H, Liu Y, Rundberg Nilsson A, Gatchalian R, Crother T, Tourtellotte W, et al. Metformin inhibition of mitochondrial ATP and DNA synthesis abrogates NLRP3 inflammasome activation and pulmonary inflammation. Immunity. 2021;54:1463-1477.e11 pubmed publisher
	Maksoud M, Tellios V, Lu W. Nitric oxide attenuates microglia proliferation by sequentially facilitating calcium influx through TRPV2 channels, activating NFATC2, and increasing p21 transcription. Cell Cycle. 2021;20:417-433 pubmed publisher
	Tyagi A, Yu M, Darby T, Vaccaro C, Li J, Owens J, et al. The Microbial Metabolite Butyrate Stimulates Bone Formation via T Regulatory Cell-Mediated Regulation of WNT10B Expression. Immunity. 2018;49:1116-1131.e7 pubmed publisher
	Sarikhani M, Mishra S, Maity S, Kotyada C, Wolfgeher D, Gupta M, et al. SIRT2 deacetylase regulates the activity of GSK3 isoforms independent of inhibitory phosphorylation. elife. 2018;7: pubmed publisher
	Alam M, Gaida M, Debnath S, Tagad H, Miller Jenkins L, Appella E, et al. Unique properties of TCR-activated p38 are necessary for NFAT-dependent T-cell activation. PLoS Biol. 2018;16:e2004111 pubmed publisher
	Wang P, Wang L, Chen L, Sun X. Dual-specificity tyrosine-phosphorylation regulated kinase 1A Gene Transcription is regulated by Myocyte Enhancer Factor 2D. Sci Rep. 2017;7:7240 pubmed publisher
	Ma B, Yu J, Xie C, Sun L, Lin S, Ding J, et al. Toll-Like Receptors Promote Mitochondrial Translocation of Nuclear Transcription Factor Nuclear Factor of Activated T-Cells in Prolonged Microglial Activation. J Neurosci. 2015;35:10799-814 pubmed publisher
	Torgerson T, Genin A, Chen C, Zhang M, Zhou B, Anover Sombke S, et al. FOXP3 inhibits activation-induced NFAT2 expression in T cells thereby limiting effector cytokine expression. J Immunol. 2009;183:907-15 pubmed publisher
	Wu W, Misra R, Russell J, Flavell R, Rincon M, Budd R. Proteolytic regulation of nuclear factor of activated T (NFAT) c2 cells and NFAT activity by caspase-3. J Biol Chem. 2006;281:10682-90 pubmed
	Kim H, Korn L, Gamero A, Leonard W. Calcium-dependent activation of interleukin-21 gene expression in T cells. J Biol Chem. 2005;280:25291-7 pubmed
	Minami T, Horiuchi K, Miura M, Abid M, Takabe W, Noguchi N, et al. Vascular endothelial growth factor- and thrombin-induced termination factor, Down syndrome critical region-1, attenuates endothelial cell proliferation and angiogenesis. J Biol Chem. 2004;279:50537-54 pubmed
	Barlic J, McDermott D, Merrell M, Gonzales J, Via L, Murphy P. Interleukin (IL)-15 and IL-2 reciprocally regulate expression of the chemokine receptor CX3CR1 through selective NFAT1- and NFAT2-dependent mechanisms. J Biol Chem. 2004;279:48520-34 pubmed
	Frazer Abel A, Baksh S, Fosmire S, Willis D, Pierce A, Meylemans H, et al. Nicotine activates nuclear factor of activated T cells c2 (NFATc2) and prevents cell cycle entry in T cells. J Pharmacol Exp Ther. 2004;311:758-69 pubmed
	Rosenberg P, Hawkins A, Stiber J, Shelton J, Hutcheson K, Bassel Duby R, et al. TRPC3 channels confer cellular memory of recent neuromuscular activity. Proc Natl Acad Sci U S A. 2004;101:9387-92 pubmed
	Kim H, Leonard W. The basis for TCR-mediated regulation of the IL-2 receptor alpha chain gene: role of widely separated regulatory elements. EMBO J. 2002;21:3051-9 pubmed
	Zhou B, Cron R, Wu B, Genin A, Wang Z, Liu S, et al. Regulation of the murine Nfatc1 gene by NFATc2. J Biol Chem. 2002;277:10704-11 pubmed
	Schubert L, Cron R, Cleary A, Brunner M, Song A, Lu L, et al. A T cell-specific enhancer of the human CD40 ligand gene. J Biol Chem. 2002;277:7386-95 pubmed
	Kehoe K, Brown M, Imani F. Double-stranded RNA regulates IL-4 expression. J Immunol. 2001;167:2496-501 pubmed
	Plyte S, Boncristiano M, Fattori E, Galvagni F, Paccani S, Majolini M, et al. Identification and characterization of a novel nuclear factor of activated T-cells-1 isoform expressed in mouse brain. J Biol Chem. 2001;276:14350-8 pubmed
	Swoap S, Hunter R, Stevenson E, Felton H, Kansagra N, Lang J, et al. The calcineurin-NFAT pathway and muscle fiber-type gene expression. Am J Physiol Cell Physiol. 2000;279:C915-24 pubmed
	Chow C, Rincon M, Cavanagh J, Dickens M, Davis R. Nuclear accumulation of NFAT4 opposed by the JNK signal transduction pathway. Science. 1997;278:1638-41 pubmed
	Ho A, Jain J, Rao A, Hogan P. Expression of the transcription factor NFATp in a neuronal cell line and in the murine nervous system. J Biol Chem. 1994;269:28181-6 pubmed

product information

Product Type :

Antibody

Product Name :

NFATC2 Monoclonal Antibody (25A10.D6.D2)

Catalog # :

MA1-025

Quantity :

100 ug

Price :

US 491.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Human, Mouse, Rat

Applications :

ChIP Assay: 1-3 uL, Gel Shift: Assay-dependent, Immunocytochemistry: 1:100-1:1,000, Immunohistochemistry (Frozen): 1:50-1:200, Immunohistochemistry (Paraffin): 1:50-1:200, Immunoprecipitation: Assay-dependent, Western Blot: 1 ug/mL

Species :

Human, Mouse, Rat

Clone :

25A10.D6.D2

Isotype :

IgG1

Storage :

-20 C, Avoid Freeze/Thaw Cycles

Description :

The nuclear factor of activated T-cells (NFAT) transcription complex is required for the expression of a group of proteins that collectively regulate the immune response. Four NFAT proteins, encoded on separate genes and expressed as several splice variants, have been described: NFAT1 (also known as NFATp or NFATc2), NFAT2 (NFATc or NFATc1), NFAT3, and NFAT4 (NFATx or NFATc3). These proteins show a low level of sequence similarity with the Dorsal/Rel/NFkB family of transcription factors. Another NFAT-related protein termed NFAT5 differs from isoforms 1-4 in that it lacks many of the Fos/Jun contact sites observed in its predecessors and its subcellular localization is not calcineurin-dependent.

Immunogen :

Synthetic peptide corresponding to residues A(51) I S S P S G L A Y P D D V L D Y G L(69) of mouse NFATc2-A, B and C isoforms.

Format :

Liquid

Applications w/Dilutions :

Aliases :

AI607462; IB-IXL; KIAA0611; NFAT pre-existing s; NFAT pre-existing subunit; NFAT transcription complex, preexisting component; Nfat1; NFAT1-D; Nfatc2; NF-ATc2; Nfatp; NF-ATp; nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 2; nuclear factor of activated T-cells 2; nuclear factor of activated T-cells, cytoplasmic 2; nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2; nuclear factor of activated T-cells, preexisting component; OTTHUMP00000031291; preexisting nuclear factor of activated T-cells 2; T cell transcription factor NFAT1; T-cell transcription factor NFAT1

more info or order :

Invitrogen product webpage