TRA-1-60 Monoclonal Antibody (TRA-1-60) | Invitrogen MA1-023 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

TRA-1-60 Monoclonal Antibody (TRA-1-60)

catalog :

MA1-023

quantity :

100 µg

price :

US 372.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

TRA-1-60

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry

more info or order :

Invitrogen product webpage

citations: 42

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; 1:1000; loading ...	Qanash H, Li Y, Smith R, Linask K, Young Baird S, Hakami W, et al. Eltrombopag Improves Erythroid Differentiation in a Human Induced Pluripotent Stem Cell Model of Diamond Blackfan Anemia. Cells. 2021;10: pubmed publisher
immunohistochemistry; human; 1:50; fig 1c	Weltner J, Balboa D, Katayama S, Bespalov M, Krjutskov K, Jouhilahti E, et al. Human pluripotent reprogramming with CRISPR activators. Nat Commun. 2018;9:2643 pubmed publisher
immunocytochemistry; mouse; 1:500; loading ...; fig s1c	Achuta V, Grym H, Putkonen N, Louhivuori V, Kärkkäinen V, Koistinaho J, et al. Metabotropic glutamate receptor 5 responses dictate differentiation of neural progenitors to NMDA-responsive cells in fragile X syndrome. Dev Neurobiol. 2017;77:438-453 pubmed publisher
immunocytochemistry; human; 1:500	Trokovic R, Weltner J, Noisa P, Raivio T, Otonkoski T. Combined negative effect of donor age and time in culture on the reprogramming efficiency into induced pluripotent stem cells. Stem Cell Res. 2015;15:254-62 pubmed publisher
immunocytochemistry; human; 1:500; fig 1	Trokovic R, Weltner J, Otonkoski T. Generation of iPSC line HEL47.2 from healthy human adult fibroblasts. Stem Cell Res. 2015;15:263-5 pubmed publisher
immunocytochemistry; human; 1:500; fig 1a	Trokovic R, Weltner J, Otonkoski T. Generation of iPSC line HEL24.3 from human neonatal foreskin fibroblasts. Stem Cell Res. 2015;15:266-8 pubmed publisher
	Su P, Lee W, Zernant J, Tsang S, Nagasaki T, Corneo B, et al. Establishment of the iPSC line CUIMCi005-A from a patient with Stargardt disease for retinal organoid culture. Stem Cell Res. 2022;65:102973 pubmed publisher
	Jalil S, Keskinen T, Maldonado R, Sokka J, Trokovic R, Otonkoski T, et al. Simultaneous high-efficiency base editing and reprogramming of patient fibroblasts. Stem Cell Reports. 2021;16:3064-3075 pubmed publisher
	Rodriguez Polo I, Mißbach S, Petkov S, Mattern F, Maierhofer A, Grządzielewska I, et al. A piggyBac-based platform for genome editing and clonal rhesus macaque iPSC line derivation. Sci Rep. 2021;11:15439 pubmed publisher
	Catlett T, Onesto M, McCann A, Rempel S, Glass J, Franz D, et al. RHOA signaling defects result in impaired axon guidance in iPSC-derived neurons from patients with tuberous sclerosis complex. Nat Commun. 2021;12:2589 pubmed publisher
	Kim M, Hwang S, Yoon J, Jeong Y, Kim E, Hyun S. Optimized Approaches for the Induction of Putative Canine Induced Pluripotent Stem Cells from Old Fibroblasts Using Synthetic RNAs. Animals (Basel). 2020;10: pubmed publisher
	Kim K, Wu Y, Yoon J, Adachi K, Wu G, Velychko S, et al. Reprogramming competence of OCT factors is determined by transactivation domains. Sci Adv. 2020;6: pubmed publisher
	Castro Viñuelas R, Sanjurjo Rodríguez C, Piñeiro Ramil M, Rodríguez Fernández S, Fuentes Boquete I, Blanco F, et al. Generation of a human control iPS cell line (ESi080-A) from a donor with no rheumatic diseases. Stem Cell Res. 2020;43:101683 pubmed publisher
	Saito K, Iioka H, Maruyama S, Sumardika I, Sakaguchi M, Kondo E. PODXL1 promotes metastasis of the pancreatic ductal adenocarcinoma by activating the C5aR/C5a axis from the tumor microenvironment. Neoplasia. 2019;21:1121-1132 pubmed publisher
	Vosough M, Ravaioli F, Zabulica M, Capri M, Garagnani P, Franceschi C, et al. Applying hydrodynamic pressure to efficiently generate induced pluripotent stem cells via reprogramming of centenarian skin fibroblasts. PLoS ONE. 2019;14:e0215490 pubmed publisher
	Nakanishi M, Mitchell R, Benoit Y, Orlando L, Reid J, Shimada K, et al. Human Pluripotency Is Initiated and Preserved by a Unique Subset of Founder Cells. Cell. 2019;177:910-924.e22 pubmed publisher
	Mair B, Tomic J, Masud S, Tonge P, Weiss A, Usaj M, et al. Essential Gene Profiles for Human Pluripotent Stem Cells Identify Uncharacterized Genes and Substrate Dependencies. Cell Rep. 2019;27:599-615.e12 pubmed publisher
	Ebrahimi A, Sevinç K, Gürhan Sevinç G, Cribbs A, Philpott M, Uyulur F, et al. Bromodomain inhibition of the coactivators CBP/EP300 facilitate cellular reprogramming. Nat Chem Biol. 2019;15:519-528 pubmed publisher
	Linker S, Urban L, Clark S, Chhatriwala M, Amatya S, McCarthy D, et al. Combined single-cell profiling of expression and DNA methylation reveals splicing regulation and heterogeneity. Genome Biol. 2019;20:30 pubmed publisher
	Li J, Narayanan C, Bian J, Sambo D, Brickler T, Zhang W, et al. A transient DMSO treatment increases the differentiation potential of human pluripotent stem cells through the Rb family. PLoS ONE. 2018;13:e0208110 pubmed publisher
	Lo Sardo V, Chubukov P, Ferguson W, Kumar A, Teng E, Duran M, et al. Unveiling the Role of the Most Impactful Cardiovascular Risk Locus through Haplotype Editing. Cell. 2018;175:1796-1810.e20 pubmed publisher
	Balboa D, Saarimäki Vire J, Borshagovski D, Survila M, Lindholm P, Galli E, et al. Insulin mutations impair beta-cell development in a patient-derived iPSC model of neonatal diabetes. elife. 2018;7: pubmed publisher
	Kutsche L, Gysi D, Fallmann J, Lenk K, Petri R, Swiersy A, et al. Combined Experimental and System-Level Analyses Reveal the Complex Regulatory Network of miR-124 during Human Neurogenesis. Cell Syst. 2018;7:438-452.e8 pubmed publisher
	Rosati J, Ferrari D, Altieri F, Tardivo S, Ricciolini C, Fusilli C, et al. Establishment of stable iPS-derived human neural stem cell lines suitable for cell therapies. Cell Death Dis. 2018;9:937 pubmed publisher
	Karch C, Hernández D, Wang J, Marsh J, Hewitt A, Hsu S, et al. Human fibroblast and stem cell resource from the Dominantly Inherited Alzheimer Network. Alzheimers Res Ther. 2018;10:69 pubmed publisher
	Shaltouki A, Hsieh C, Kim M, Wang X. Alpha-synuclein delays mitophagy and targeting Miro rescues neuron loss in Parkinson's models. Acta Neuropathol. 2018;136:607-620 pubmed publisher
	Riesenberg S, Maricic T. Targeting repair pathways with small molecules increases precise genome editing in pluripotent stem cells. Nat Commun. 2018;9:2164 pubmed publisher
	Ali M, Khan S, Vasanth S, Ahmed M, Chen R, Na C, et al. Generation and Proteome Profiling of PBMC-Originated, iPSC-Derived Corneal Endothelial Cells. Invest Ophthalmol Vis Sci. 2018;59:2437-2444 pubmed publisher
	Pastor W, Liu W, Chen D, Ho J, Kim R, Hunt T, et al. TFAP2C regulates transcription in human naive pluripotency by opening enhancers. Nat Cell Biol. 2018;20:553-564 pubmed publisher
	Thekkeparambil Chandrabose S, Sriram S, Subramanian S, Cheng S, Ong W, Rozen S, et al. Amenable epigenetic traits of dental pulp stem cells underlie high capability of xeno-free episomal reprogramming. Stem Cell Res Ther. 2018;9:68 pubmed publisher
	Kogut I, McCarthy S, Pavlova M, Astling D, Chen X, Jakimenko A, et al. High-efficiency RNA-based reprogramming of human primary fibroblasts. Nat Commun. 2018;9:745 pubmed publisher
	Victor M, Richner M, Olsen H, Lee S, Monteys A, Ma C, et al. Striatal neurons directly converted from Huntington's disease patient fibroblasts recapitulate age-associated disease phenotypes. Nat Neurosci. 2018;21:341-352 pubmed publisher
	Melguizo Sanchís D, Xu Y, Taheem D, Yu M, Tilgner K, Bárta T, et al. iPSC modeling of severe aplastic anemia reveals impaired differentiation and telomere shortening in blood progenitors. Cell Death Dis. 2018;9:128 pubmed publisher
	Achuta V, Möykkynen T, Peteri U, Turconi G, Rivera C, Keinanen K, et al. Functional changes of AMPA responses in human induced pluripotent stem cell-derived neural progenitors in fragile X syndrome. Sci Signal. 2018;11: pubmed publisher
	Ho J, Cattoglio C, McSwiggen D, Tjian R, Fong Y. Regulation of DNA demethylation by the XPC DNA repair complex in somatic and pluripotent stem cells. Genes Dev. 2017;31:830-844 pubmed publisher
	Allison R, Edgar J, Pearson G, Rizo T, Newton T, GÃ¼nther S, et al. Defects in ER-endosome contacts impact lysosome function in hereditary spastic paraplegia. J Cell Biol. 2017;216:1337-1355 pubmed publisher
	Cooper H, Yang Y, Ylikallio E, Khairullin R, Woldegebriel R, Lin K, et al. ATPase-deficient mitochondrial inner membrane protein ATAD3A disturbs mitochondrial dynamics in dominant hereditary spastic paraplegia. Hum Mol Genet. 2017;26:1432-1443 pubmed publisher
	Saini J, Corneo B, Miller J, Kiehl T, Wang Q, Boles N, et al. Nicotinamide Ameliorates Disease Phenotypes in a Human iPSC Model of Age-Related Macular Degeneration. Cell Stem Cell. 2017;20:635-647.e7 pubmed publisher
	Trevisan M, Desole G, Costanzi G, Lavezzo E, Palu G, Barzon L. Reprogramming Methods Do Not Affect Gene Expression Profile of Human Induced Pluripotent Stem Cells. Int J Mol Sci. 2017;18: pubmed publisher
	Quintanilla R, Asprer J, Sylakowski K, Lakshmipathy U. Kinetic Measurement and Real Time Visualization of Somatic Reprogramming. J Vis Exp. 2016;: pubmed publisher
	Ghosh K, Selokar N, Gahlawat S, Kumar D, Kumar P, Yadav P. Amnion Epithelial Cells of Buffalo (Bubalus Bubalis) Term Placenta Expressed Embryonic Stem Cells Markers and Differentiated into Cells of Neurogenic Lineage In Vitro. Anim Biotechnol. 2016;27:38-43 pubmed publisher
	Han H, Irimia M, Ross P, Sung H, Alipanahi B, David L, et al. MBNL proteins repress ES-cell-specific alternative splicing and reprogramming. Nature. 2013;498:241-5 pubmed publisher

image

image 1 :

Immunofluorescent analysis of TRA-1-60 using anti-TRA-1-60 monoclonal antibody (Product# MA1-023) shows staining on the cell surface of human HEL 11.4 iPS cells, indicating pluripotency. TRA-1-60 staining (green) and an overlay image of TRA-1-60 with DAPI (blue) is shown. HEL 11.4 iPS cells were grown on matrigel coated chamber slides and fixed with formaldehyde prior to staining. Cells were probed with a Monoclonal antibody recognizing TRA-1-60 (Product# MA1-023) at a dilution of 1:50 over night at 4 ºC, washed with PBS and incubated with a fluorescein conjugated secondary antibody at a dilution of 1:100 for 60 minutes at room temperature. Images were taken at 20X magnification.

image 2 :

Flow cytometry analysis of TRA-1-60 using anti-TRA-1-60 monoclonal antibody (Product# MA1-023) shows positive staining of H9 human embryonic stem cells (blue line). H9 human embryonic stem cells were harvested, fixed and washed with PBS. Cells were incubated with anti-TRA-1-60 monoclonal antibody (Product# MA1-023) or control at a 1:100 dilution for 1 hour on ice, followed by 30 min incubation with fluorescein conjugated secondary antibody. 100,000 cells were stained for each sample.

image 3 :

Western blot analysis of TRA-1-60 was performed by loading 100 µg of NTERA-2 whole cell lysate onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. Membranes were probed with a mouse monoclonal antibody recognizing TRA-1-60 (Product# MA1-023) at a dilution of 1:500 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-IgM HRP secondary antibody at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed using Pierce Super Signal West Dura (Product #34075).

product information

Product Type :

Antibody

Product Name :

TRA-1-60 Monoclonal Antibody (TRA-1-60)

Catalog # :

MA1-023

Quantity :

100 µg

Price :

US 372.00

Clonality :

Monoclonal

Purity :

Affinity chromatography - MBP

Host :

Mouse

Reactivity :

Human

Applications :

Flow Cytometry: 0.4 µg, Immunocytochemistry: 1:20-1:100, Western Blot: 1:500-1:2,000

Species :

Human

Clone :

TRA-1-60

Isotype :

IgM

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

TRA-1-60 is a cell surface antigen, expressed along with SSEA-3, SSEA-4 and TRA-1-81 in human embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). These surface markers are lost during the differentiation process. In contrast, SSEA-1 is absent in undifferentiated human stem cells but is present on the cell surface after retinoic acid mediated differentiation.

Immunogen :

Human embryonal carcinoma cell line 2102Ep.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 0.4 µg, Immunocytochemistry: 1:20-1:100, Western Blot: 1:500-1:2,000

Aliases :

TRA160; tra60

more info or order :

Invitrogen product webpage