SSEA3 Monoclonal Antibody (MC-631) | Invitrogen MA1-020 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

SSEA3 Monoclonal Antibody (MC-631)

catalog :

MA1-020

quantity :

100 µg

price :

US 395

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

MC-631

reactivity :

human

application :

immunocytochemistry, flow cytometry

more info or order :

Invitrogen product webpage

citations: 6

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; 1:500	Brandl C, Zimmermann S, Milenkovic V, Rosendahl S, Grassmann F, Milenkovic A, et al. In-depth characterisation of Retinal Pigment Epithelium (RPE) cells derived from human induced pluripotent stem cells (hiPSC). Neuromolecular Med. 2014;16:551-64 pubmed publisher
	Castro Viñuelas R, Sanjurjo Rodríguez C, Piñeiro Ramil M, Rodríguez Fernández S, Fuentes Boquete I, Blanco F, et al. Generation of a human control iPS cell line (ESi080-A) from a donor with no rheumatic diseases. Stem Cell Res. 2020;43:101683 pubmed publisher
	Leng Z, Sun D, Huang Z, Tadmori I, Chiang N, Kethidi N, et al. Quantitative Analysis of SSEA3+ Cells from Human Umbilical Cord after Magnetic Sorting. Cell Transplant. 2019;28:907-923 pubmed publisher
	Hildebrandt T, Hermes R, Colleoni S, Diecke S, Holtze S, Renfree M, et al. Embryos and embryonic stem cells from the white rhinoceros. Nat Commun. 2018;9:2589 pubmed publisher
	Cheung S, Chuang P, Huang H, Hwang Verslues W, Cho C, Yang W, et al. Stage-specific embryonic antigen-3 (SSEA-3) and Î²3GalT5 are cancer specific and significant markers for breast cancer stem cells. Proc Natl Acad Sci U S A. 2016;113:960-5 pubmed publisher
	Johansson M, Dedic B, Lundholm K, Branzell F, Barone A, Benktander J, et al. Characterization of moose intestinal glycosphingolipids. Glycoconj J. 2015;32:393-412 pubmed publisher

image

image 1 :

Immunofluorescent analysis of SSEA-3 using anti-SSEA-3 monoclonal antibody (Product# MA1-020) shows staining on the cell surface of human HEL 11.4 iPS cells, indicating pluripotency. SSEA-3 staining (green) and an overlay image of SSEA-3 with DAPI (blue) is shown. HEL 11.4 iPS cells were grown on matrigel coated chamber slides and fixed with formaldehyde prior to staining. Cells were probed with a rat monoclonal antibody recognizing SSEA-3 (Product# MA1-020) at a dilution of 1:20 overnight at 4 ºC, washed with PBS and incubated with a fluorescein conjugated secondary antibody at a dilution of 1:100 for 60 minutes at room temperature. Images were taken at 20X magnification.

image 2 :

Flow cytometry analysis of SSEA-3 using anti-SSEA-3 monoclonal antibody (Product# MA1-020) shows positive staining of H9 human embryonic stem cells (blue line). H9 human embryonic stem cells were harvested, fixed and washed with PBS. Cells were incubated with anti-SSEA-3 monoclonal antibody (Product# MA1-020) or control at a 1:100 dilution for 1 hour on ice, followed by 30 min incubation with fluorescein conjugated secondary antibody. 100,000 cells were stained for each sample.

product information

Product Type :

Antibody

Product Name :

SSEA3 Monoclonal Antibody (MC-631)

Catalog # :

MA1-020

Quantity :

100 µg

Price :

US 395

Clonality :

Monoclonal

Purity :

Affinity chromatography - MBP

Host :

Rat

Reactivity :

Human

Applications :

Flow Cytometry: 1:100, Immunocytochemistry: 1:100-1:500

Species :

Human

Clone :

MC-631

Isotype :

IgM

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

SSEA-3 (stage-specific embryonic antigen 3) is a cell surface antigen, expressed along with SSEA-4, TRA-1-60 and TRA-1-81 in embryonic stem cells, embryonal carcinoma cells, and induced pluripotent stem cells (iPS). In human cells, these surface markers are down-regulated during the differentiation process, while SSEA1 is absent in undifferentiated human stem cells and upregulated on the cell surface after retinoic acid mediated differentiation. Conversely, in mouse stem cells, SSEA-1 expression decreases with differentiation while SSEA-3 and SSEA-4 expression increase.

Immunogen :

Fischer rat immunized with 4-8 cell stage mouse embryos

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 1:100, Immunocytochemistry: 1:100-1:500

Aliases :

SSEA-3; stage-specific embryonic antigen 3

more info or order :

Invitrogen product webpage