Nanog Monoclonal Antibody (23D2-3C6) | Invitrogen MA1-017 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Nanog Monoclonal Antibody (23D2-3C6)

catalog :

MA1-017

quantity :

100 ug

price :

US 414.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

23D2-3C6

reactivity :

human

application :

western blot, immunocytochemistry, flow cytometry

more info or order :

Invitrogen product webpage

citations: 11

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; loading ...; fig s1b	Sivakumar S, Qi S, Cheng N, Sathe A, Kanchwala M, Kumar A, et al. TP53 promotes lineage commitment of human embryonic stem cells through ciliogenesis and sonic hedgehog signaling. Cell Rep. 2022;38:110395 pubmed publisher
immunocytochemistry; human; 1:100; loading ...; fig 1f	Jung Klawitter S, Ebersold J, GÃ¶hring G, Blau N, Opladen T. Generation of an iPSC line from a patient with GTP cyclohydrolase 1 (GCH1) deficiency: HDMC0061i-GCH1. Stem Cell Res. 2017;20:38-41 pubmed publisher
immunocytochemistry; human; 1:100; loading ...; fig 1d	Jung Klawitter S, Blau N, Sebe A, Ebersold J, GÃ¶hring G, Opladen T. Generation of an iPSC line from a patient with tyrosine hydroxylase (TH) deficiency: TH-1 iPSC. Stem Cell Res. 2016;17:580-583 pubmed publisher
western blot; human; 1:1000; fig s2	Vijaya Chandra S, Makhija H, Peter S, Myint Wai C, Li J, Zhu J, et al. Conservative site-specific and single-copy transgenesis in human LINE-1 elements. Nucleic Acids Res. 2016;44:e55 pubmed publisher
western blot; human; fig 4a	Wongtrakoongate P, Riddick G, Fucharoen S, Felsenfeld G. Association of the Long Non-coding RNA Steroid Receptor RNA Activator (SRA) with TrxG and PRC2 Complexes. PLoS Genet. 2015;11:e1005615 pubmed publisher
immunocytochemistry; human; 1:100; fig 2h	Balboa D, Weltner J, Eurola S, Trokovic R, Wartiovaara K, Otonkoski T. Conditionally Stabilized dCas9 Activator for Controlling Gene Expression in Human Cell Reprogramming and Differentiation. Stem Cell Reports. 2015;5:448-59 pubmed publisher
	Kettunen P, Lesnikova A, R xe4 s xe4 nen N, Ojha R, Palmunen L, Laakso M, et al. SARS-CoV-2 Infection of Human Neurons Is TMPRSS2 Independent, Requires Endosomal Cell Entry, and Can Be Blocked by Inhibitors of Host Phosphoinositol-5 Kinase. J Virol. 2023;97:e0014423 pubmed publisher
	Kumar S, De Leon E, Granados J, Whitworth D, Vandeberg J. Monodelphis domestica Induced Pluripotent Stem Cells Reveal Metatherian Pluripotency Architecture. Int J Mol Sci. 2022;23: pubmed publisher
	Nieto Est xe9 vez V, Donegan J, McMahon C, Elam H, Chavera T, Varma P, et al. Buprenorphine Exposure Alters the Development and Migration of Interneurons in the Cortex. Front Mol Neurosci. 2022;15:889922 pubmed publisher
	Yoshida S, Okura H, Suga H, Soen M, Kawaguchi Y, Kurimoto J, et al. Generation of four induced pluripotent stem cell lines (FHUi003-A, FHUi003-B, FHUi004-A and FHUi004-B) from two affected individuals of a familial neurohypophyseal diabetes insipidus family. Stem Cell Res. 2020;48:101960 pubmed publisher
	Triantafillu U, Park S, Klaassen N, Raddatz A, Kim Y. Fluid shear stress induces cancer stem cell-like phenotype in MCF7 breast cancer cell line without inducing epithelial to mesenchymal transition. Int J Oncol. 2017;50:993-1001 pubmed publisher

product information

Product Type :

Antibody

Product Name :

Nanog Monoclonal Antibody (23D2-3C6)

Catalog # :

MA1-017

Quantity :

100 ug

Price :

US 414.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Human

Applications :

Flow Cytometry: Assay-dependent, Immunocytochemistry: 1:50 -1:100, Western Blot: 1:500-1:2,000

Species :

Human

Clone :

23D2-3C6

Isotype :

IgG1

Storage :

-20 C

Description :

NANOG (Nanog homeobox) is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. NANOG mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Human NANOG protein shares 52% overall amino acid identity with the mouse protein, and 85% identity in the homeodomain. Human NANOG maps to gene locus 12p13.31, while the mouse NANOG maps to gene loci 6 F2. Murine embryonic NANOG expression is detected in the inner cell mass of the blastocyst. Research studies have shown that high levels of human NANOG expression in the undifferentiated N-Tera embryonal carcinoma cell line. Further, NANOG is a transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. The role of NANOG in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 (also known as Oct-4), another germline-specific transcription factor, and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. Experiments have demonstrated that iPS cells could be generated using expression plasmids expressing NANOG, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine. When overexpressed, NANOG promotes cells to enter into S phase and proliferation. Diseases associated with dysfunction in the NANOG protein include tetracarcinoma and germ cell/embryonal cancer.

Immunogen :

Full-length human recombinant protein expressed in bacteria

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: Assay-dependent, Immunocytochemistry: 1:50 -1:100, Western Blot: 1:500-1:2,000

Aliases :

2410002E02Rik; early embryo specific expression NK family; early embryo specific expression NK-type homeobox protein; Ecat4; ENK; ES cell-associated protein 4; FLJ12581; HGNC:20857; hNanog; Homeobox protein NANOG; homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; Nanog; Nanog homeobox

more info or order :

Invitrogen product webpage