Pan Ras Monoclonal Antibody (Ras10) | Invitrogen MA1-012X product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Pan Ras Monoclonal Antibody (Ras10)

catalog :

MA1-012X

quantity :

20 µg

price :

US 175

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

RAS10

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 17

Published Application/Species/Sample/Dilution	Reference
immunoprecipitation; human; 1:50; loading ...; fig 1d	Umstead M, Xiong J, Qi Q, Du Y, Fu H. Aurora kinase A interacts with H-Ras and potentiates Ras-MAPK signaling. Oncotarget. 2017;8:28359-28372 pubmed publisher
western blot; mouse; fig 3c	Riecken L, Tawamie H, Dornblut C, Buchert R, Ismayel A, Schulz A, et al. Inhibition of RAS activation due to a homozygous ezrin variant in patients with profound intellectual disability. Hum Mutat. 2015;36:270-8 pubmed publisher
	Inoue D, Polaski J, Taylor J, Castel P, Chen S, Kobayashi S, et al. Minor intron retention drives clonal hematopoietic disorders and diverse cancer predisposition. Nat Genet. 2021;53:707-718 pubmed publisher
	Lau H, Tang J, Casey P, Wang M. Evaluating the Epithelial-Mesenchymal Program in Human Breast Epithelial Cells Cultured in Soft Agar Using a Novel Macromolecule Extraction Protocol. Cancers (Basel). 2021;13: pubmed publisher
	Liotti F, Kumar N, Prevete N, Marotta M, Sorriento D, Ierano C, et al. PD-1 blockade delays tumor growth by inhibiting an intrinsic SHP2/Ras/MAPK signalling in thyroid cancer cells. J Exp Clin Cancer Res. 2021;40:22 pubmed publisher
	Jilek J, Tu M, Zhang C, Yu A. Pharmacokinetic and Pharmacodynamic Factors Contribute to Synergism between Let-7c-5p and 5-Fluorouracil in Inhibiting Hepatocellular Carcinoma Cell Viability. Drug Metab Dispos. 2020;48:1257-1263 pubmed publisher
	Beckwitt C, Shiraha K, Wells A. Lipophilic statins limit cancer cell growth and survival, via involvement of Akt signaling. PLoS ONE. 2018;13:e0197422 pubmed publisher
	Scavello M, Petlick A, Ramesh R, Thompson V, Lotfi P, Charest P. Protein kinase A regulates the Ras, Rap1 and TORC2 pathways in response to the chemoattractant cAMP in Dictyostelium. J Cell Sci. 2017;130:1545-1558 pubmed publisher
	Strazza M, Azoulay Alfaguter I, Peled M, Smrcka A, Skolnik E, Srivastava S, et al. PLCÎµ1 regulates SDF-1Î±-induced lymphocyte adhesion and migration to sites of inflammation. Proc Natl Acad Sci U S A. 2017;114:2693-2698 pubmed publisher
	Lau H, Tang J, Casey P, Wang M. Isoprenylcysteine carboxylmethyltransferase is critical for malignant transformation and tumor maintenance by all RAS isoforms. Oncogene. 2017;36:3934-3942 pubmed publisher
	Shi Q, Li C, Li K, Liu Q. Pallidin protein in neurodevelopment and its relation to the pathogenesis of schizophrenia. Mol Med Rep. 2017;15:665-672 pubmed publisher
	Raffeiner P, Schraffl A, Schwarz T, RÃ¶ck R, Ledolter K, Hartl M, et al. Calcium-dependent binding of Myc to calmodulin. Oncotarget. 2017;8:3327-3343 pubmed publisher
	Mitra S, Ghosh B, Gayen N, Roy J, Mandal A. Bipartite Role of Heat Shock Protein 90 (Hsp90) Keeps CRAF Kinase Poised for Activation. J Biol Chem. 2016;291:24579-24593 pubmed
	Shi D, Shi G, Xie J, Du X, Yang H. MicroRNA-27a Inhibits Cell Migration and Invasion of Fibroblast-Like Synoviocytes by Targeting Follistatin-Like Protein 1 in Rheumatoid Arthritis. Mol Cells. 2016;39:611-8 pubmed publisher
	Lau H, Ramanujulu P, Guo D, Yang T, Wirawan M, Casey P, et al. An improved isoprenylcysteine carboxylmethyltransferase inhibitor induces cancer cell death and attenuates tumor growth in vivo. Cancer Biol Ther. 2014;15:1280-91 pubmed publisher
	Figueras A, Arbos M, Quiles M, Vinals F, Germà J, Capella G. The impact of KRAS mutations on VEGF-A production and tumour vascular network. BMC Cancer. 2013;13:125 pubmed publisher
	Hamer P, Trimpe K, Pullano T, Ng S, LaVecchio J, Petit D, et al. Production and characterization of anti-RAS p21 monoclonal antibodies. Hybridoma. 1990;9:573-87 pubmed

image

image 1 :

Immunofluorescent analysis of Ras in HeLa Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Ras monoclonal antibody (Product # MA1-012) at a dilution of 1:100 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). Ras staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

image 2 :

Immunohistochemistry was performed on cancer biopsies of deparaffinized Human colon carcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and probed with a Ras monoclonal antibody (Product # MA1-012) at a dilution of 1:200 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

image 3 :

Western blot analysis of Ras was performed by loading 50ug of various whole cell lysate onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were then probed with a mouse monoclonal antibody recognizing Ras (Product # MA1-012) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were then washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody (Product #31430) at a dilution of 1:20000 for at least one hour. Membranes were washed and chemiluminescent detection was performed using Pierce Super Signal West Pico(Product #34077).

product information

Product Type :

Antibody

Product Name :

Pan Ras Monoclonal Antibody (Ras10)

Catalog # :

MA1-012X

Quantity :

20 µg

Price :

US 175

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Human, Mouse, Non-human primate

Applications :

Flow Cytometry: Assay-dependent, Immunocytochemistry: 1:50-1:200, Immunohistochemistry (Paraffin): 1:100-1:500, Immunoprecipitation: 2 µg, Western Blot: 1:1,000

Species :

Human, Mouse, Non-human primate

Clone :

Ras10

Isotype :

IgG2a

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

This gene belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, mental retardation, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Multiple transcript variants, which encode different isoforms, have been identified for this gene.

Immunogen :

Purified recombinant Ras protein

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: Assay-dependent, Immunocytochemistry: 1:50-1:200, Immunohistochemistry (Paraffin): 1:100-1:500, Immunoprecipitation: 2 µg, Western Blot: 1:1,000

Aliases :

AI929937; ALPS4; AV095280; C-BAS/HAS; cellular c-Ki-ras2; cellular c-Ki-ras2 proto-oncogene; CFC2; c-Ha-ras; c-Ha-ras p21 protein; c-Ha-ras transgene; C-HA-RAS1; c-has/bas p21 protein; C-H-RAS; c-Ki-ras; c-Kirsten-ras protein; c-Kirsten-ras proto-oncogene; c-K-ras; c-K-ras2 protein; CMNS; c-rasHa; c-ras-Ki-2 activated oncogene; CTLO; etID18709.8; fa04e08; fc14b12; fc23g10; fj89d12; fj99a10; GTP- and GDP-binding peptide B; GTPase HRas; GTPase HRas, N-terminally processed; GTPase Hras1; GTPase KRas; GTPase KRas, N-terminally processed; GTPase NRas; HAMSV; Ha-ras; Ha-Ras1 proto-oncoprotein; Harvey ras1 protein; Harvey rat sarcoma viral (v-Ha-ras) oncogene homolog; Harvey rat sarcoma viral oncogene homolog; Harvey rat sarcoma viral oncoprotein; Harvey rat sarcoma virus oncogene; Harvey rat sarcoma virus oncogene 1; Harvey-ras; hras; H-ras; H-ras 1 protein; HRas proto-oncogene, GTPase; HRas proto-oncogene, GTPase a; HRAS1; H-Ras-1; Hras-1; hrasa; H-RASIDX; hypothetical protein LOC445289; hypothetical protein LOC550286; K RAS; Ki-ras; Kirsten rat sarcoma oncogene 2, expressed; Kirsten rat sarcoma viral oncogene; Kirsten rat sarcoma viral oncogene homolog; Kirsten rat sarcoma viral oncogene homologue 2 (active); Kras; K-ras; K-Ras 2; K-ras p21 protein; KRAS proto-oncogene, GTPase; KRAS1; KRAS2; Kras-2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; NCMS; Neuroblastoma RAS; neuroblastoma ras oncogene; neuroblastoma RAS viral (v-ras) oncogene homolog; neuroblastoma RAS viral oncogene homolog; NRAS; N-ras; N-ras oncogene p21; N-ras protein part 4; NRAS proto-oncogene, GTPase; NRAS1; NS; NS3; NS6; oncogene KRAS2; p19 H-RasIDX protein; p21; p21 N-ras oncogene; p21 protein; p21B; p21ras; PR310 c-K-ras oncogene; RALD; ras; Ras family small GTP binding protein H-Ras; RASH1; RASK; RASK2; ras-like protein; RASN; sb:eu826; transformation gene: oncogene HAMSV; transforming protein N-Ras; Transforming protein p21; v-Ha-ras Harvey rat sarcoma viral oncogene homolog; v-Ha-ras Harvey rat sarcoma viral oncogene homolog a; v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog; v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog; v-ras neuroblastoma RAS viral oncogene homolog; wu:fa04e08; wu:fc14b12; wu:fc23g10; wu:fj89d12; wu:fj99a10; xRAS1; xRAS2; zgc:100761; zgc:110250; zgc:85725; zras-b1

more info or order :

Invitrogen product webpage