product summary
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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
IFN gamma Monoclonal Antibody (2G1)
catalog :
M700A
quantity :
500 µg
price :
US 514
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
2G1
reactivity :
human, mouse
application :
western blot, ELISA, immunohistochemistry, immunoprecipitation, blocking or activating experiments, other
more info or order :
citations: 231
| Published Application/Species/Sample/Dilution | Reference |
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| Carroll R, Troelnikov A, Chong A. Virtual Global Transplant Laboratory Standard Operating Protocol for Donor Alloantigen-specific Interferon-gamma ELISPOT Assay. Transplant Direct. 2016;2:e111 pubmed
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| Berinstein N, Karkada M, Oza A, Odunsi K, Villella J, Nemunaitis J, et al. Survivin-targeted immunotherapy drives robust polyfunctional T cell generation and differentiation in advanced ovarian cancer patients. Oncoimmunology. 2015;4:e1026529 pubmed
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| Di J, Massuger L, Duiveman de Boer T, Zusterzeel P, Figdor C, Torensma R. Functional OCT4-specific CD4+ and CD8+ T cells in healthy controls and ovarian cancer patients. Oncoimmunology. 2013;2:e24271 pubmed
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| Spring M, Chelimo K, Tisch D, Sumba P, Rochford R, Long C, et al. Allele specificity of gamma interferon responses to the carboxyl-terminal region of Plasmodium falciparum merozoite surface protein 1 by Kenyan adults with naturally acquired immunity to malaria. Infect Immun. 2010;78:4431-41 pubmed publisher
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| Simpson Abelson M, Sonnenberg G, Takita H, Yokota S, Conway T, Kelleher R, et al. Long-term engraftment and expansion of tumor-derived memory T cells following the implantation of non-disrupted pieces of human lung tumor into NOD-scid IL2Rgamma(null) mice. J Immunol. 2008;180:7009-18 pubmed
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| Maccalli C, Nonaka D, Piris A, Pende D, Rivoltini L, Castelli C, et al. NKG2D-mediated antitumor activity by tumor-infiltrating lymphocytes and antigen-specific T-cell clones isolated from melanoma patients. Clin Cancer Res. 2007;13:7459-68 pubmed
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| Intapan P, Kittimongkolma S, Niwattayakul K, Sawanyawisuth K, Maleewong W. Cerebrospinal fluid cytokine responses in human eosinophilic meningitis associated with angiostrongyliasis. J Neurol Sci. 2008;267:17-21 pubmed
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| Windhagen A, Sonmez D, Hornig Do H, Kalinowsky A, Schwinzer R. Altered CD45 isoform expression in C77G carriers influences cytokine responsiveness and adhesion properties of T cells. Clin Exp Immunol. 2007;150:509-17 pubmed
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| Nowacki T, Kuerten S, Zhang W, Shive C, Kreher C, Boehm B, et al. Granzyme B production distinguishes recently activated CD8(+) memory cells from resting memory cells. Cell Immunol. 2007;247:36-48 pubmed
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| Morse M, Hobeika A, Osada T, Niedzwiecki D, Marcom P, Blackwell K, et al. Long term disease-free survival and T cell and antibody responses in women with high-risk Her2+ breast cancer following vaccination against Her2. J Transl Med. 2007;5:42 pubmed
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| Eksioglu E, Mahmood S, Chang M, Reddy V. GM-CSF promotes differentiation of human dendritic cells and T lymphocytes toward a predominantly type 1 proinflammatory response. Exp Hematol. 2007;35:1163-71 pubmed
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| Smith J, Joseph H, Green T, Field J, Wooters M, Kaufhold R, et al. Establishing acceptance criteria for cell-mediated-immunity assays using frozen peripheral blood mononuclear cells stored under optimal and suboptimal conditions. Clin Vaccine Immunol. 2007;14:527-37 pubmed
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| Yonkers N, Rodriguez B, Milkovich K, Asaad R, Lederman M, Heeger P, et al. TLR ligand-dependent activation of naive CD4 T cells by plasmacytoid dendritic cells is impaired in hepatitis C virus infection. J Immunol. 2007;178:4436-44 pubmed
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| Moormann A, Chelimo K, Sumba P, Tisch D, Rochford R, Kazura J. Exposure to holoendemic malaria results in suppression of Epstein-Barr virus-specific T cell immunosurveillance in Kenyan children. J Infect Dis. 2007;195:799-808 pubmed
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| Fonseca S, Coutinho Silva A, Fonseca L, Segurado A, Moraes S, Rodrigues H, et al. Identification of novel consensus CD4 T-cell epitopes from clade B HIV-1 whole genome that are frequently recognized by HIV-1 infected patients. AIDS. 2006;20:2263-73 pubmed
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| Kitawaki T, Kadowaki N, Sugimoto N, Kambe N, Hori T, Miyachi Y, et al. IgE-activated mast cells in combination with pro-inflammatory factors induce Th2-promoting dendritic cells. Int Immunol. 2006;18:1789-99 pubmed
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| Baek M, Park H, Johnson J, Altuntas C, Jane wit D, Jaini R, et al. Increased frequencies of cochlin-specific T cells in patients with autoimmune sensorineural hearing loss. J Immunol. 2006;177:4203-10 pubmed
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| Kotton C, Lankowski A, Scott N, Sisul D, Chen L, Raschke K, et al. Safety and immunogenicity of attenuated Salmonella enterica serovar Typhimurium delivering an HIV-1 Gag antigen via the Salmonella Type III secretion system. Vaccine. 2006;24:6216-24 pubmed
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| Moormann A, John C, Sumba P, Tisch D, Embury P, Kazura J. Stability of interferon-gamma and interleukin-10 responses to Plasmodium falciparum liver stage antigen-1 and thrombospondin-related adhesive protein in residents of a malaria holoendemic area. Am J Trop Med Hyg. 2006;74:585-90 pubmed
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| Alleva D, Maki R, Putnam A, Robinson J, Kipnes M, Dandona P, et al. Immunomodulation in type 1 diabetes by NBI-6024, an altered peptide ligand of the insulin B epitope. Scand J Immunol. 2006;63:59-69 pubmed
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| Nagarajan S, Selvaraj P. Human tumor membrane vesicles modified to express glycolipid-anchored IL-12 by protein transfer induce T cell proliferation in vitro: a potential approach for local delivery of cytokines during vaccination. Vaccine. 2006;24:2264-74 pubmed
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| Kleen T, Kadereit S, Fanning L, Jaroscak J, Fu P, Meyerson H, et al. Recipient-specific tolerance after HLA-mismatched umbilical cord blood stem cell transplantation. Transplantation. 2005;80:1316-22 pubmed
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| Kuekrek H, Schlingmann T, Valdez H, Boehm B, Pollard R, Mitsuyasu R, et al. Differential effect of interleukin-2 treatment on primary and secondary immunizations in HIV infected individuals. AIDS. 2005;19:1967-74 pubmed
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| Quast S, Zhang W, Shive C, Kovalovski D, Ott P, Herzog B, et al. IL-2 absorption affects IFN-gamma and IL-5, but not IL-4 producing memory T cells in double color cytokine ELISPOT assays. Cell Immunol. 2005;237:28-36 pubmed
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| Carranza C, Juarez E, Torres M, Ellner J, Sada E, Schwander S. Mycobacterium tuberculosis growth control by lung macrophages and CD8 cells from patient contacts. Am J Respir Crit Care Med. 2006;173:238-45 pubmed
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| Berner B, Tary Lehmann M, Yonkers N, Askari A, Lehmann P, Anthony D. Phenotypic and functional analysis of EBV-specific memory CD8 cells in SLE. Cell Immunol. 2005;235:29-38 pubmed
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| Lyke K, Burges R, Cissoko Y, Sangare L, Kone A, Dao M, et al. HLA-A2 supertype-restricted cell-mediated immunity by peripheral blood mononuclear cells derived from Malian children with severe or uncomplicated Plasmodium falciparum malaria and healthy controls. Infect Immun. 2005;73:5799-808 pubmed
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| Reyes B, Danese S, Sans M, Fiocchi C, Levine A. Redox equilibrium in mucosal T cells tunes the intestinal TCR signaling threshold. J Immunol. 2005;175:2158-66 pubmed
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| Groh M, Maitra B, Szekely E, Koc O. Human mesenchymal stem cells require monocyte-mediated activation to suppress alloreactive T cells. Exp Hematol. 2005;33:928-34 pubmed
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| Della Bella S, Riva A, Tanzi E, Nicola S, Amendola A, Vecchi L, et al. Hepatitis C virus-specific reactivity of CD4+-lymphocytes in children born from HCV-infected women. J Hepatol. 2005;43:394-402 pubmed
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| Konur A, Schulz U, Eissner G, Andreesen R, Holler E. Interferon (IFN)-gamma is a main mediator of keratinocyte (HaCaT) apoptosis and contributes to autocrine IFN-gamma and tumour necrosis factor-alpha production. Br J Dermatol. 2005;152:1134-42 pubmed
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| Cox A, Mosbruger T, Mao Q, Liu Z, Wang X, Yang H, et al. Cellular immune selection with hepatitis C virus persistence in humans. J Exp Med. 2005;201:1741-52 pubmed
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| Salerno Goncalves R, Wahid R, Sztein M. Immunization of volunteers with Salmonella enterica serovar Typhi strain Ty21a elicits the oligoclonal expansion of CD8+ T cells with predominant Vbeta repertoires. Infect Immun. 2005;73:3521-30 pubmed
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| Malhotra I, Mungai P, Muchiri E, Ouma J, Sharma S, Kazura J, et al. Distinct Th1- and Th2-Type prenatal cytokine responses to Plasmodium falciparum erythrocyte invasion ligands. Infect Immun. 2005;73:3462-70 pubmed
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| Yamshchikov G, Mullins D, Chang C, Ogino T, Thompson L, Presley J, et al. Sequential immune escape and shifting of T cell responses in a long-term survivor of melanoma. J Immunol. 2005;174:6863-71 pubmed
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| Trudeau C, Cotreau M, Stonis L, Dykstra K, Oestreicher J, Strahs A, et al. A single administration of recombinant human interleukin-12 is associated with increased expression levels of interferon-gamma and signal transducer and activator of transcription in healthy subjects. J Clin Pharmacol. 2005;45:649-58 pubmed
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| Ott P, Herzog B, Quast S, Hofstetter H, Boehm B, Tary Lehmann M, et al. Islet-cell antigen-reactive T cells show different expansion rates and Th1/Th2 differentiation in type 1 diabetic patients and healthy controls. Clin Immunol. 2005;115:102-14 pubmed
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| Baecher Allan C, Wolf E, Hafler D. Functional analysis of highly defined, FACS-isolated populations of human regulatory CD4+ CD25+ T cells. Clin Immunol. 2005;115:10-8 pubmed
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| Fonseca S, Moins Teisserenc H, Clave E, Ianni B, Nunes V, Mady C, et al. Identification of multiple HLA-A*0201-restricted cruzipain and FL-160 CD8+ epitopes recognized by T cells from chronically Trypanosoma cruzi-infected patients. Microbes Infect. 2005;7:688-97 pubmed
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| Sabel M, Hess S, Egilmez N, Conway T, Chen F, Bankert R. CTLA-4 blockade augments human T lymphocyte-mediated suppression of lung tumor xenografts in SCID mice. Cancer Immunol Immunother. 2005;54:944-52 pubmed
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| Kaufhold R, Field J, Caulfield M, Wang S, Joseph H, Wooters M, et al. Memory T-cell response to rotavirus detected with a gamma interferon enzyme-linked immunospot assay. J Virol. 2005;79:5684-94 pubmed
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| Walrath J, Zukowski L, Krywiak A, Silver R. Resident Th1-like effector memory cells in pulmonary recall responses to Mycobacterium tuberculosis. Am J Respir Cell Mol Biol. 2005;33:48-55 pubmed
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| Sugiyama T, Gursel M, Takeshita F, Coban C, Conover J, Kaisho T, et al. CpG RNA: identification of novel single-stranded RNA that stimulates human CD14+CD11c+ monocytes. J Immunol. 2005;174:2273-9 pubmed
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| Skarsvik S, Ludvigsson J, Vaarala O. Aberrant regulation of interleukin-12 receptor beta2 chain on type 1 cytokine-stimulated T lymphocytes in type 1 diabetes. Immunology. 2005;114:287-93 pubmed
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| Broderick L, Yokota S, Reineke J, Mathiowitz E, Stewart C, Barcos M, et al. Human CD4+ effector memory T cells persisting in the microenvironment of lung cancer xenografts are activated by local delivery of IL-12 to proliferate, produce IFN-gamma, and eradicate tumor cells. J Immunol. 2005;174:898-906 pubmed
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| Tambur A, Ben Ari Z, Herrera N, Klein T, Michowiz R, Mor E. Donor-specific hyporesponsiveness in ELISPOT assay is associated with early recurrence of hepatitis C in liver transplant recipients. Hum Immunol. 2005;66:21-7 pubmed
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| Parekh K, Ramachandran S, Cooper J, Bigner D, Patterson A, Mohanakumar T. Tenascin-C, over expressed in lung cancer down regulates effector functions of tumor infiltrating lymphocytes. Lung Cancer. 2005;47:17-29 pubmed
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| Kondo E, Akatsuka Y, Nawa A, Kuzushima K, Tsujimura K, Tanimoto M, et al. Retroviral vector backbone immunogenicity: identification of cytotoxic T-cell epitopes in retroviral vector-packaging sequences. Gene Ther. 2005;12:252-8 pubmed
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| Meloni F, Cascina A, Paschetto E, Marone Bianco A, Morosini M, Pellegrini C, et al. Monocyte chemoattractant protein-1 levels in bronchoalveolar lavage fluid of lung-transplanted patients treated with tacrolimus as rescue treatment for refractory acute rejection. Transplant Proc. 2003;35:1523-6 pubmed
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van Beek J, Gorris M, Sköld A, Hatipoglu I, Van Acker H, Smits E, et al. Human blood myeloid and plasmacytoid dendritic cells cross activate each other and synergize in inducing NK cell cytotoxicity. Oncoimmunology. 2016;5:e1227902 pubmed
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Szmacinski H, Toshchakov V, Piao W, Lakowicz J. Imaging of Protein Secretion from a Single Cell Using Plasmonic Substrates. Bionanoscience. 2013;3:30-36 pubmed
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Demachi Okamura A, Ito Y, Akatsuka Y, Tsujimura K, Morishima Y, Takahashi T, et al. Epstein-Barr virus (EBV) latent membrane protein-1-specific cytotoxic T lymphocytes targeting EBV-carrying natural killer cell malignancies. Eur J Immunol. 2006;36:593-602 pubmed
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Guthmann M, Bitton R, Carnero A, Gabri M, Cinat G, Koliren L, et al. Active specific immunotherapy of melanoma with a GM3 ganglioside-based vaccine: a report on safety and immunogenicity. J Immunother. 2004;27:442-51 pubmed
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de la Barrera S, Aleman M, Musella R, Schierloh P, Pasquinelli V, Garcia V, et al. IL-10 down-regulates costimulatory molecules on Mycobacterium tuberculosis-pulsed macrophages and impairs the lytic activity of CD4 and CD8 CTL in tuberculosis patients. Clin Exp Immunol. 2004;138:128-38 pubmed
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John C, Moormann A, Sumba P, Ofulla A, Pregibon D, Kazura J. Gamma interferon responses to Plasmodium falciparum liver-stage antigen 1 and thrombospondin-related adhesive protein and their relationship to age, transmission intensity, and protection against malaria. Infect Immun. 2004;72:5135-42 pubmed
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Pohjavuori E, Viljanen M, Korpela R, Kuitunen M, Tiittanen M, Vaarala O, et al. Lactobacillus GG effect in increasing IFN-gamma production in infants with cow's milk allergy. J Allergy Clin Immunol. 2004;114:131-6 pubmed
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Ott P, Dittrich M, Herzog B, Guerkov R, Gottlieb P, Putnam A, et al. T cells recognize multiple GAD65 and proinsulin epitopes in human type 1 diabetes, suggesting determinant spreading. J Clin Immunol. 2004;24:327-39 pubmed
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Nissinen R, Leirisalo Repo M, Peltomaa R, Palosuo T, Vaarala O. Cytokine and chemokine receptor profile of peripheral blood mononuclear cells during treatment with infliximab in patients with active rheumatoid arthritis. Ann Rheum Dis. 2004;63:681-7 pubmed
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Lovett Racke A, Hussain R, Northrop S, Choy J, Rocchini A, Matthes L, et al. Peroxisome proliferator-activated receptor alpha agonists as therapy for autoimmune disease. J Immunol. 2004;172:5790-8 pubmed
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Kleen T, Asaad R, Landry S, Boehm B, Tary Lehmann M. Tc1 effector diversity shows dissociated expression of granzyme B and interferon-gamma in HIV infection. AIDS. 2004;18:383-92 pubmed
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Kaufmann D, Bailey P, Sidney J, Wagner B, Norris P, Johnston M, et al. Comprehensive analysis of human immunodeficiency virus type 1-specific CD4 responses reveals marked immunodominance of gag and nef and the presence of broadly recognized peptides. J Virol. 2004;78:4463-77 pubmed
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Kadereit S, Junge G, Kleen T, Kozik M, Kaminski B, Daum Woods K, et al. Deficient IFN-gamma expression in umbilical cord blood (UCB) T cells can be rescued by IFN-gamma-mediated increase in NFATc2 expression. J Clin Immunol. 2003;23:485-97 pubmed
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Schotte H, Schluter B, Willeke P, Mickholz E, Schorat M, Domschke W, et al. Long-term treatment with etanercept significantly reduces the number of proinflammatory cytokine-secreting peripheral blood mononuclear cells in patients with rheumatoid arthritis. Rheumatology (Oxford). 2004;43:960-4 pubmed
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Martins T, Pasi B, Litwin C, Hill H. Heterophile antibody interference in a multiplexed fluorescent microsphere immunoassay for quantitation of cytokines in human serum. Clin Diagn Lab Immunol. 2004;11:325-9 pubmed
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Ott P, Berner B, Herzog B, Guerkov R, Yonkers N, Durinovic Bello I, et al. CD28 costimulation enhances the sensitivity of the ELISPOT assay for detection of antigen-specific memory effector CD4 and CD8 cell populations in human diseases. J Immunol Methods. 2004;285:223-35 pubmed
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Rasmussen A, Zocca M, Bonefeld C, von Essen M, Lauritsen J, Tomra S, et al. Proteasomal targeting and minigene repetition improve cell-surface presentation of a transfected, modified melanoma tumour antigen. Scand J Immunol. 2004;59:220-7 pubmed
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Harashima N, Kurihara K, Utsunomiya A, Tanosaki R, Hanabuchi S, Masuda M, et al. Graft-versus-Tax response in adult T-cell leukemia patients after hematopoietic stem cell transplantation. Cancer Res. 2004;64:391-9 pubmed
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Maitra B, Szekely E, Gjini K, Laughlin M, Dennis J, Haynesworth S, et al. Human mesenchymal stem cells support unrelated donor hematopoietic stem cells and suppress T-cell activation. Bone Marrow Transplant. 2004;33:597-604 pubmed
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Manfras B, Reuter S, Wendland T, Boehm B, Kern P. Impeded Th1 CD4 memory T cell generation in chronic-persisting liver infection with Echinococcus multilocularis. Int Immunol. 2004;16:43-50 pubmed
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Tambur A. Monitoring indirect presentation of alloantigens by utilizing the autologous processing machinery of dendritic cells in-vitro. J Immunol Methods. 2003;283:215-23 pubmed
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Guerkov R, Targoni O, Kreher C, Boehm B, Herrera M, Tary Lehmann M, et al. Detection of low-frequency antigen-specific IL-10-producing CD4(+) T cells via ELISPOT in PBMC: cognate vs. nonspecific production of the cytokine. J Immunol Methods. 2003;279:111-21 pubmed
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Kreher C, Dittrich M, Guerkov R, Boehm B, Tary Lehmann M. CD4+ and CD8+ cells in cryopreserved human PBMC maintain full functionality in cytokine ELISPOT assays. J Immunol Methods. 2003;278:79-93 pubmed
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Kondo E, Akatsuka Y, Kuzushima K, Tsujimura K, Asakura S, Tajima K, et al. Identification of novel CTL epitopes of CMV-pp65 presented by a variety of HLA alleles. Blood. 2004;103:630-8 pubmed
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Malhotra I, Ouma J, Wamachi A, Kioko J, Mungai P, Njzovu M, et al. Influence of maternal filariasis on childhood infection and immunity to Wuchereria bancrofti in Kenya. Infect Immun. 2003;71:5231-7 pubmed
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Mannering S, Purcell A, Honeyman M, McCluskey J, Harrison L. Human T-cells recognise N-terminally Fmoc-modified peptide. Vaccine. 2003;21:3638-46 pubmed
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Maccalli C, Pende D, Castelli C, Mingari M, Robbins P, Parmiani G. NKG2D engagement of colorectal cancer-specific T cells strengthens TCR-mediated antigen stimulation and elicits TCR independent anti-tumor activity. Eur J Immunol. 2003;33:2033-43 pubmed
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Vincenti D, Carrara S, De Mori P, Pucillo L, Petrosillo N, Palmieri F, et al. Identification of early secretory antigen target-6 epitopes for the immunodiagnosis of active tuberculosis. Mol Med. 2003;9:105-11 pubmed
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Solomon S, Masilamani M, Mohanty S, Schwab J, Boneberg E, Illges H. Generation of three-dimensional pannus-like tissues in vitro from single cell suspensions of synovial fluid cells from arthritis patients. Rheumatol Int. 2004;24:71-6 pubmed
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Delcayre A, Peake J, White D, Yuan S, McDonald M, Liang A, et al. A genome-based functional screening approach to vaccine development that combines in vitro assays and DNA immunization. Vaccine. 2003;21:3259-64 pubmed
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Tsunoda M, Litonjua A, Kuniak M, Weiss S, Satoh T, Guevarra L, et al. Serum cytokine levels, cigarette smoking and airway responsiveness among pregnant women. Int Arch Allergy Immunol. 2003;130:158-64 pubmed
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Moore M, Kurago Z, Fullenkamp C, Lutz C. Squamous cell carcinoma cells differentially stimulate NK cell effector functions: the role of IL-18. Cancer Immunol Immunother. 2003;52:107-15 pubmed
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Salerno Goncalves R, Wyant T, Pasetti M, Fernandez Vina M, Tacket C, Levine M, et al. Concomitant induction of CD4+ and CD8+ T cell responses in volunteers immunized with Salmonella enterica serovar typhi strain CVD 908-htrA. J Immunol. 2003;170:2734-41 pubmed
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Steel C, Nutman T. CTLA-4 in filarial infections: implications for a role in diminished T cell reactivity. J Immunol. 2003;170:1930-8 pubmed
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Hess S, Egilmez N, Bailey N, Anderson T, Mathiowitz E, Bernstein S, et al. Human CD4+ T cells present within the microenvironment of human lung tumors are mobilized by the local and sustained release of IL-12 to kill tumors in situ by indirect effects of IFN-gamma. J Immunol. 2003;170:400-12 pubmed
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Sumimoto H, Tsuji T, Miyoshi H, Hagihara M, Takada Yamazaki R, Okamoto S, et al. Rapid and efficient generation of lentivirally gene-modified dendritic cells from DC progenitors with bone marrow stromal cells. J Immunol Methods. 2002;271:153-65 pubmed
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Baecher Allan C, Viglietta V, Hafler D. Inhibition of human CD4(+)CD25(+high) regulatory T cell function. J Immunol. 2002;169:6210-7 pubmed
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Oosterwijk Wakka J, Tiemessen D, Bleumer I, de Vries I, Jongmans W, Adema G, et al. Vaccination of patients with metastatic renal cell carcinoma with autologous dendritic cells pulsed with autologous tumor antigens in combination with interleukin-2: a phase 1 study. J Immunother. 2002;25:500-8 pubmed
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Jaramillo A, Majumder K, Manna P, Fleming T, Doherty G, DiPersio J, et al. Identification of HLA-A3-restricted CD8+ T cell epitopes derived from mammaglobin-A, a tumor-associated antigen of human breast cancer. Int J Cancer. 2002;102:499-506 pubmed
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Montenegro S, Abath F, Domingues A, Melo W, Morais C, Coutinho E, et al. Enhanced interleukin-12 and CD40 ligand activities but reduced Staphylococcus aureus Cowan 1-induced responses suggest a generalized and progressively impaired type 1 cytokine pattern for human schistosomiasis. Infect Immun. 2002;70:5903-12 pubmed
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Yun P, Decarlo A, Collyer C, Hunter N. Modulation of an interleukin-12 and gamma interferon synergistic feedback regulatory cycle of T-cell and monocyte cocultures by Porphyromonas gingivalis lipopolysaccharide in the absence or presence of cysteine proteinases. Infect Immun. 2002;70:5695-705 pubmed
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Pelfrey C, Cotleur A, Lee J, Rudick R. Sex differences in cytokine responses to myelin peptides in multiple sclerosis. J Neuroimmunol. 2002;130:211-23 pubmed
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Lorenz R, Solares C, Williams P, Sikora J, Pelfrey C, Hughes G, et al. Interferon-gamma production to inner ear antigens by T cells from patients with autoimmune sensorineural hearing loss. J Neuroimmunol. 2002;130:173-8 pubmed
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Xainli J, Baisor M, Kastens W, Bockarie M, Adams J, King C. Age-dependent cellular immune responses to Plasmodium vivax Duffy binding protein in humans. J Immunol. 2002;169:3200-7 pubmed
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Salerno Goncalves R, Pasetti M, Sztein M. Characterization of CD8(+) effector T cell responses in volunteers immunized with Salmonella enterica serovar Typhi strain Ty21a typhoid vaccine. J Immunol. 2002;169:2196-203 pubmed
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Angelakopoulos H, Loock K, Sisul D, Jensen E, Miller J, Hohmann E. Safety and shedding of an attenuated strain of Listeria monocytogenes with a deletion of actA/plcB in adult volunteers: a dose escalation study of oral inoculation. Infect Immun. 2002;70:3592-601 pubmed
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Nagarajan S, Selvaraj P. Glycolipid-anchored IL-12 expressed on tumor cell surface induces antitumor immune response. Cancer Res. 2002;62:2869-74 pubmed
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Komatsu N, Shichijo S, Maeda Y, Itoh K. Measurement of interferon-gamma by high-throughput fluorometric microvolume assay technology system. J Immunol Methods. 2002;263:169-76 pubmed
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Libraty D, Endy T, Houng H, Green S, Kalayanarooj S, Suntayakorn S, et al. Differing influences of virus burden and immune activation on disease severity in secondary dengue-3 virus infections. J Infect Dis. 2002;185:1213-21 pubmed
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Wurtzen P, Claesson M. A HLA-A2 restricted human CTL line recognizes a novel tumor cell expressed p53 epitope. Int J Cancer. 2002;99:568-72 pubmed
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Egilmez N, Hess S, Chen F, Takita H, Conway T, Bankert R. Human CD4+ effector T cells mediate indirect interleukin-12- and interferon-gamma-dependent suppression of autologous HLA-negative lung tumor xenografts in severe combined immunodeficient mice. Cancer Res. 2002;62:2611-7 pubmed
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Eusebius N, Papalia L, Suphioglu C, McLellan S, Varney M, Rolland J, et al. Oligoclonal analysis of the atopic T cell response to the group 1 allergen of Cynodon dactylon (bermuda grass) pollen: pre- and post-allergen-specific immunotherapy. Int Arch Allergy Immunol. 2002;127:234-44 pubmed
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Wiesemann E, Sönmez D, Heidenreich F, Windhagen A. Interferon-beta increases the stimulatory capacity of monocyte-derived dendritic cells to induce IL-13, IL-5 and IL-10 in autologous T-cells. J Neuroimmunol. 2002;123:160-9 pubmed
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Hancock G, Scheuer C, Sierzega R, Pryharski K, McBride J, Watelet L, et al. Adaptive immune responses of patients with asthma to the attachment (G) glycoprotein of respiratory synctial virus. J Infect Dis. 2001;184:1589-93 pubmed
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King C, Malhotra I, Wamachi A, Kioko J, Mungai P, Wahab S, et al. Acquired immune responses to Plasmodium falciparum merozoite surface protein-1 in the human fetus. J Immunol. 2002;168:356-64 pubmed
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Daubenberger C, Salomon M, Vecino W, Hubner B, Troll H, Rodriques R, et al. Functional and structural similarity of V gamma 9V delta 2 T cells in humans and Aotus monkeys, a primate infection model for Plasmodium falciparum malaria. J Immunol. 2001;167:6421-30 pubmed
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Hioe C, Tuen M, Chien P, Jones G, Ratto Kim S, Norris P, et al. Inhibition of human immunodeficiency virus type 1 gp120 presentation to CD4 T cells by antibodies specific for the CD4 binding domain of gp120. J Virol. 2001;75:10950-7 pubmed
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Kellar K, Kalwar R, Dubois K, Crouse D, Chafin W, Kane B. Multiplexed fluorescent bead-based immunoassays for quantitation of human cytokines in serum and culture supernatants. Cytometry. 2001;45:27-36 pubmed
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image
image 1 :
Purified human CD4+ T cells were left non-stimulated (Lane 1), stimulated with anti-CD3 (Lane 2), or stimulated with anti-CD3 and anti-CD28 (Lane 3) for three days. IFN? production (pg/ml) was determined by ELISA by coating M700A (1:1000), blocking with 1% BSA in PBS, and detection with M701B (1:1000), followed by peroxidase-conjugated avidin and TMB substrate. Data courtesy of Innovators Program.
product information
Product Type :
Antibody
Product Name :
IFN gamma Monoclonal Antibody (2G1)
Catalog # :
M700A
Quantity :
500 µg
Price :
US 514
Clonality :
Monoclonal
Purity :
Protein G
Host :
Mouse
Reactivity :
Human
Applications :
ELISA: Assay-dependent, Western Blot: Assay-dependent
Species :
Human
Clone :
2G1
Isotype :
IgG1, kappa
Storage :
-20°C
Description :
IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.
Immunogen :
Recombinant human IFN gamma
Format :
Liquid
Applications w/Dilutions :
ELISA: Assay-dependent, Western Blot: Assay-dependent
Aliases :
BoIFNG; cytokine; EGK_03901; gamma interferon; gamma interferon precursor; gamma-interferon; gamma-interferon precursor (AA -23 to 143); H-IFN-g; IFG; IFI; IFN gamma; IFN ?; Ifng; IFN-g; IFNG2; IFN-gamma; IFN-gamma precursor; IFN-y; IFN?; Immune interferon; interferon gamma; interferon gamma precursor; interferon gamma type 2; Interferon y; Interferon ?; interferon, gamma; interferon-gama; interferon-gamma; Interferon-gamma level; interferon-gamma precursor; Interferon?; M-IFN-g; R-IFN-g
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com81 Wyman Street
Waltham, MA USA 02451
800-678-5599
headquarters: USA
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