TNF alpha Monoclonal Antibody (2TNF-H34A) | Invitrogen M303 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

TNF alpha Monoclonal Antibody (2TNF-H34A)

catalog :

M303

quantity :

500 µg

price :

US 466

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2TNF-H34A

reactivity :

human

application :

western blot, ELISA, blocking or activating experiments

more info or order :

Invitrogen product webpage

citations: 17

Published Application/Species/Sample/Dilution	Reference
blocking or activating experiments; human; fig 4f	Ma Y, Usuwanthim K, Munawara U, Quach A, Gorgani N, Abbott C, et al. Protein kinase cÎ± regulates the expression of complement receptor Ig in human monocyte-derived macrophages. J Immunol. 2015;194:2855-61 pubmed publisher
ELISA; human	Nakamura T, Schwander S, Donnelly R, Ortega F, Togo F, Broderick G, et al. Cytokines across the night in chronic fatigue syndrome with and without fibromyalgia. Clin Vaccine Immunol. 2010;17:582-7 pubmed publisher
ELISA; human	Cencioni M, Chiurchiù V, Catanzaro G, Borsellino G, Bernardi G, Battistini L, et al. Anandamide suppresses proliferation and cytokine release from primary human T-lymphocytes mainly via CB2 receptors. PLoS ONE. 2010;5:e8688 pubmed publisher
ELISA; human; 1:5000	Kumada Y, Hamasaki K, Shiritani Y, Ohse T, Kishimoto M. Efficient immobilization of a ligand antibody with high antigen-binding activity by use of a polystyrene-binding peptide and an intelligent microtiter plate. J Biotechnol. 2009;142:135-41 pubmed publisher
ELISA; human	López Antón N, Hermann C, Murillo R, Merfort I, Wanner G, Vollmar A, et al. Sesquiterpene lactones induce distinct forms of cell death that modulate human monocyte-derived macrophage responses. Apoptosis. 2007;12:141-53 pubmed
ELISA; human; 200 ug/ml	Urbanowska T, Mangialaio S, Zickler C, Cheevapruk S, Hasler P, Regenass S, et al. Protein microarray platform for the multiplex analysis of biomarkers in human sera. J Immunol Methods. 2006;316:1-7 pubmed
ELISA; human; 0.3 ug/ml	Pedraza Sánchez S, Gonzalez Hernandez Y, Escobar Gutierrez A, Ramachandra L. The immunostimulant RU41740 from Klebsiella pneumoniae activates human cells in whole blood to potentially stimulate innate and adaptive immune responses. Int Immunopharmacol. 2006;6:635-46 pubmed
	Pedraza Sánchez S, Hise A, Ramachandra L, Arechavaleta Velasco F, King C. Reduced frequency of a CD14+ CD16+ monocyte subset with high Toll-like receptor 4 expression in cord blood compared to adult blood contributes to lipopolysaccharide hyporesponsiveness in newborns. Clin Vaccine Immunol. 2013;20:962-71 pubmed publisher
	Greig N, Giordano T, Zhu X, Yu Q, Perry T, Holloway H, et al. Thalidomide-based TNF-alpha inhibitors for neurodegenerative diseases. Acta Neurobiol Exp (Wars). 2004;64:1-9 pubmed
	Tiberio L, Fletcher L, Eldridge J, Duncan D. Host factors impacting the innate response in humans to the candidate adjuvants RC529 and monophosphoryl lipid A. Vaccine. 2004;22:1515-23 pubmed
	Schotte H, Schluter B, Willeke P, Mickholz E, Schorat M, Domschke W, et al. Long-term treatment with etanercept significantly reduces the number of proinflammatory cytokine-secreting peripheral blood mononuclear cells in patients with rheumatoid arthritis. Rheumatology (Oxford). 2004;43:960-4 pubmed
	Solomon S, Masilamani M, Mohanty S, Schwab J, Boneberg E, Illges H. Generation of three-dimensional pannus-like tissues in vitro from single cell suspensions of synovial fluid cells from arthritis patients. Rheumatol Int. 2004;24:71-6 pubmed
	Loffreda S, Muratori P, Muratori L, Mele L, Bianchi F, Lenzi M. Enhanced monocyte Th1 cytokine production in HCV-infected cryoglobulinemic patients. J Hepatol. 2003;38:230-6 pubmed
	Fonteneau J, Gilliet M, Larsson M, Dasilva I, MUNZ C, Liu Y, et al. Activation of influenza virus-specific CD4+ and CD8+ T cells: a new role for plasmacytoid dendritic cells in adaptive immunity. Blood. 2003;101:3520-6 pubmed
	Montenegro S, Abath F, Domingues A, Melo W, Morais C, Coutinho E, et al. Enhanced interleukin-12 and CD40 ligand activities but reduced Staphylococcus aureus Cowan 1-induced responses suggest a generalized and progressively impaired type 1 cytokine pattern for human schistosomiasis. Infect Immun. 2002;70:5903-12 pubmed
	Shi Y, Lutz C. Interferon--gamma control of EBV-transformed B cells: a role for CD8+ T cells that poorly kill EBV-infected cells. Viral Immunol. 2002;15:213-25 pubmed
	Marth T, Kelsall B. Regulation of interleukin-12 by complement receptor 3 signaling. J Exp Med. 1997;185:1987-95 pubmed

product information

Product Type :

Antibody

Product Name :

TNF alpha Monoclonal Antibody (2TNF-H34A)

Catalog # :

M303

Quantity :

500 µg

Price :

US 466

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Human

Applications :

ELISA: 1:25-1:3200, Western Blot: Assay-dependent

Species :

Human

Clone :

2TNF-H34A

Isotype :

IgG1, kappa

Storage :

-20°C

Description :

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

Immunogen :

Recombinant human TNF alpha

Format :

Liquid

Applications w/Dilutions :

ELISA: 1:25-1:3200, Western Blot: Assay-dependent

Aliases :

APC1 protein; Cachectin; C-domain 1; C-domain 2; cTNF; DADB-70P7.1; DIF; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; RATTNF; Tnf; TNF alpha; TNF superfamily; TNF a; TNF, macrophage-derived; TNF, monocyte-derived; TNFA; TNF-a; TNFalpha; TNF-alpha; Tnfsf1a; TNFSF2; TNFa; TNLG1F; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor alpha (cachetin); tumor necrosis factor alpha precursor; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha; tumor necrosis factor-alpha precursor; tumor-necrosis factor; tumour necrosis factor

more info or order :

Invitrogen product webpage