TNF alpha Monoclonal Antibody (2TNF-H33), Biotin | Invitrogen M302B product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

TNF alpha Monoclonal Antibody (2TNF-H33), Biotin

catalog :

M302B

quantity :

250 µg

price :

US 466

clonality :

monoclonal

host :

mouse

conjugate :

biotin

clone name :

2TNF-H33

reactivity :

human

application :

western blot, ELISA

more info or order :

Invitrogen product webpage

citations: 19

Published Application/Species/Sample/Dilution	Reference
ELISA; human	Nakamura T, Schwander S, Donnelly R, Ortega F, Togo F, Broderick G, et al. Cytokines across the night in chronic fatigue syndrome with and without fibromyalgia. Clin Vaccine Immunol. 2010;17:582-7 pubmed publisher
ELISA; human	Cencioni M, Chiurchiù V, Catanzaro G, Borsellino G, Bernardi G, Battistini L, et al. Anandamide suppresses proliferation and cytokine release from primary human T-lymphocytes mainly via CB2 receptors. PLoS ONE. 2010;5:e8688 pubmed publisher
ELISA; human; 1:5000	Kumada Y, Hamasaki K, Shiritani Y, Ohse T, Kishimoto M. Efficient immobilization of a ligand antibody with high antigen-binding activity by use of a polystyrene-binding peptide and an intelligent microtiter plate. J Biotechnol. 2009;142:135-41 pubmed publisher
ELISA; human	López Antón N, Hermann C, Murillo R, Merfort I, Wanner G, Vollmar A, et al. Sesquiterpene lactones induce distinct forms of cell death that modulate human monocyte-derived macrophage responses. Apoptosis. 2007;12:141-53 pubmed
ELISA; human	Urbanowska T, Mangialaio S, Zickler C, Cheevapruk S, Hasler P, Regenass S, et al. Protein microarray platform for the multiplex analysis of biomarkers in human sera. J Immunol Methods. 2006;316:1-7 pubmed
ELISA; human; 0.3 ug/ml	Pedraza Sánchez S, Gonzalez Hernandez Y, Escobar Gutierrez A, Ramachandra L. The immunostimulant RU41740 from Klebsiella pneumoniae activates human cells in whole blood to potentially stimulate innate and adaptive immune responses. Int Immunopharmacol. 2006;6:635-46 pubmed
	Mukaro V, Quach A, Gahan M, Boog B, Huang Z, Gao X, et al. Small tumor necrosis factor receptor biologics inhibit the tumor necrosis factor-p38 signalling axis and inflammation. Nat Commun. 2018;9:1365 pubmed publisher
	Pedraza Sánchez S, Hise A, Ramachandra L, Arechavaleta Velasco F, King C. Reduced frequency of a CD14+ CD16+ monocyte subset with high Toll-like receptor 4 expression in cord blood compared to adult blood contributes to lipopolysaccharide hyporesponsiveness in newborns. Clin Vaccine Immunol. 2013;20:962-71 pubmed publisher
	Greig N, Giordano T, Zhu X, Yu Q, Perry T, Holloway H, et al. Thalidomide-based TNF-alpha inhibitors for neurodegenerative diseases. Acta Neurobiol Exp (Wars). 2004;64:1-9 pubmed
	Tiberio L, Fletcher L, Eldridge J, Duncan D. Host factors impacting the innate response in humans to the candidate adjuvants RC529 and monophosphoryl lipid A. Vaccine. 2004;22:1515-23 pubmed
	Schotte H, Schluter B, Willeke P, Mickholz E, Schorat M, Domschke W, et al. Long-term treatment with etanercept significantly reduces the number of proinflammatory cytokine-secreting peripheral blood mononuclear cells in patients with rheumatoid arthritis. Rheumatology (Oxford). 2004;43:960-4 pubmed
	Zhu X, Giordano T, Yu Q, Holloway H, Perry T, Lahiri D, et al. Thiothalidomides: novel isosteric analogues of thalidomide with enhanced TNF-alpha inhibitory activity. J Med Chem. 2003;46:5222-9 pubmed
	Solomon S, Masilamani M, Mohanty S, Schwab J, Boneberg E, Illges H. Generation of three-dimensional pannus-like tissues in vitro from single cell suspensions of synovial fluid cells from arthritis patients. Rheumatol Int. 2004;24:71-6 pubmed
	Loffreda S, Muratori P, Muratori L, Mele L, Bianchi F, Lenzi M. Enhanced monocyte Th1 cytokine production in HCV-infected cryoglobulinemic patients. J Hepatol. 2003;38:230-6 pubmed
	Montenegro S, Abath F, Domingues A, Melo W, Morais C, Coutinho E, et al. Enhanced interleukin-12 and CD40 ligand activities but reduced Staphylococcus aureus Cowan 1-induced responses suggest a generalized and progressively impaired type 1 cytokine pattern for human schistosomiasis. Infect Immun. 2002;70:5903-12 pubmed
	Shi Y, Lutz C. Interferon--gamma control of EBV-transformed B cells: a role for CD8+ T cells that poorly kill EBV-infected cells. Viral Immunol. 2002;15:213-25 pubmed
	Libraty D, Pichyangkul S, Ajariyakhajorn C, Endy T, Ennis F. Human dendritic cells are activated by dengue virus infection: enhancement by gamma interferon and implications for disease pathogenesis. J Virol. 2001;75:3501-8 pubmed
	Eigler A, Matschke V, Hartmann G, Erhardt S, Boyle D, Firestein G, et al. Suppression of TNF-alpha production in human mononuclear cells by an adenosine kinase inhibitor. J Leukoc Biol. 2000;68:97-103 pubmed
	Marth T, Kelsall B. Regulation of interleukin-12 by complement receptor 3 signaling. J Exp Med. 1997;185:1987-95 pubmed

image

image 1 :

Sandwich ELISA analysis of Human TNF alpha Biotinylated MAb (Pierce product # M302B) was performed using components of Thermo Fisher Scientific Human TNF alpha Colorimetric ELISA Kit (Pierce product # EH3TNFA) by loading 50ul per well of Recombinant Human TNF alpha in octuplicate at 1000, 500, 250, 125, 62.5, 31.2, 15.6, and 0 pg/ml across a 5µg/ml rabbit anti-Human TNF pre-coated plate and incubated for 1 hour at room temperature. The plate was washed then incubated with 100 µl per well of Human TNF alpha Biotinylated MAb (M302B) in duplicate at 2, 1, 0.5, and 0.25 µg/ml for 1 hour at room temperature. The plate was washed and incubated with 100 µl per well of Ultra Streptavidin-HRP (Pierce product # N504) in all test wells at 1:1,500 for 30 minutes at room temperature. The plate was washed then incubated with 100ul per well of TMB substrate for 30 minutes at room temperature in the dark. The plate was then stopped with 0.16M sulfuric acid and absorbance was read on a spectrophotometer at 450-550nm.

product information

Product Type :

Antibody

Product Name :

TNF alpha Monoclonal Antibody (2TNF-H33), Biotin

Catalog # :

M302B

Quantity :

250 µg

Price :

US 466

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Human

Applications :

ELISA: Assay-dependent, Western Blot: Assay-dependent

Species :

Human

Clone :

2TNF-H33

Isotype :

IgG1, kappa

Storage :

-20°C

Description :

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

Immunogen :

Recombinant human TNF alpha

Format :

Liquid

Applications w/Dilutions :

ELISA: Assay-dependent, Western Blot: Assay-dependent

Aliases :

APC1 protein; Cachectin; C-domain 1; C-domain 2; cTNF; DADB-70P7.1; DIF; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; RATTNF; Tnf; TNF alpha; TNF superfamily; TNF a; TNF, macrophage-derived; TNF, monocyte-derived; TNFA; TNF-a; TNFalpha; TNF-alpha; Tnfsf1a; TNFSF2; TNFa; TNLG1F; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor alpha (cachetin); tumor necrosis factor alpha precursor; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha; tumor necrosis factor-alpha precursor; tumor-necrosis factor; tumour necrosis factor

more info or order :

Invitrogen product webpage