IL-10 Monoclonal Antibody (12G8), Biotin | Invitrogen M011B product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

IL-10 Monoclonal Antibody (12G8), Biotin

catalog :

M011B

quantity :

250 µg

price :

US 430.00

clonality :

monoclonal

host :

rat

conjugate :

biotin

clone name :

12G8

reactivity :

human

application :

western blot, ELISA

more info or order :

Invitrogen product webpage

citations: 19

Published Application/Species/Sample/Dilution	Reference
ELISA; human; 1:1,000	Gregori S, Roncarolo M, Bacchetta R. Methods for in vitro generation of human type 1 regulatory T cells. Methods Mol Biol. 2011;677:31-46 pubmed publisher
ELISA; human	Bacchetta R, Gregori S, Serafini G, Sartirana C, Schulz U, Zino E, et al. Molecular and functional characterization of allogantigen-specific anergic T cells suitable for cell therapy. Haematologica. 2010;95:2134-43 pubmed publisher
ELISA; human	Eksioglu E, Bess J, Zhu H, Xu Y, Dong H, Elyar J, et al. Hepatitis C virus modulates human monocyte-derived dendritic cells. J Viral Hepat. 2010;17:757-69 pubmed publisher
ELISA; human	Serafini G, Andreani M, Testi M, Battarra M, Bontadini A, Biral E, et al. Type 1 regulatory T cells are associated with persistent split erythroid/lymphoid chimerism after allogeneic hematopoietic stem cell transplantation for thalassemia. Haematologica. 2009;94:1415-26 pubmed publisher
ELISA; human	Lee T, Leu S, Huang J, Song Y, Jhou R, Tang S, et al. Anti-ribosomal phosphoprotein autoantibody triggers interleukin-10 overproduction via phosphatidylinositol 3-kinase-dependent signalling pathways in lipopolysaccharide-activated macrophages. Immunology. 2009;127:91-102 pubmed publisher
ELISA; human	Della Bella S, Riva A, Tanzi E, Nicola S, Amendola A, Vecchi L, et al. Hepatitis C virus-specific reactivity of CD4+-lymphocytes in children born from HCV-infected women. J Hepatol. 2005;43:394-402 pubmed
ELISA; human; fig 1	Suwa T, Hogg J, Vincent R, Mukae H, Fujii T, van Eeden S. Ambient air particulates stimulate alveolar macrophages of smokers to promote differentiation of myeloid precursor cells. Exp Lung Res. 2002;28:1-18 pubmed
	Schotte H, Schluter B, Willeke P, Mickholz E, Schorat M, Domschke W, et al. Long-term treatment with etanercept significantly reduces the number of proinflammatory cytokine-secreting peripheral blood mononuclear cells in patients with rheumatoid arthritis. Rheumatology (Oxford). 2004;43:960-4 pubmed
	Della Bella S, Gennaro M, Vaccari M, Ferraris C, Nicola S, Riva A, et al. Altered maturation of peripheral blood dendritic cells in patients with breast cancer. Br J Cancer. 2003;89:1463-72 pubmed
	Meloni F, Cascina A, Paschetto E, Marone Bianco A, Morosini M, Pellegrini C, et al. Monocyte chemoattractant protein-1 levels in bronchoalveolar lavage fluid of lung-transplanted patients treated with tacrolimus as rescue treatment for refractory acute rejection. Transplant Proc. 2003;35:1523-6 pubmed
	Solomon S, Masilamani M, Mohanty S, Schwab J, Boneberg E, Illges H. Generation of three-dimensional pannus-like tissues in vitro from single cell suspensions of synovial fluid cells from arthritis patients. Rheumatol Int. 2004;24:71-6 pubmed
	Loffreda S, Muratori P, Muratori L, Mele L, Bianchi F, Lenzi M. Enhanced monocyte Th1 cytokine production in HCV-infected cryoglobulinemic patients. J Hepatol. 2003;38:230-6 pubmed
	McDowell M, Marovich M, Lira R, Braun M, Sacks D. Leishmania priming of human dendritic cells for CD40 ligand-induced interleukin-12p70 secretion is strain and species dependent. Infect Immun. 2002;70:3994-4001 pubmed
	Zarzaur B, Wu Y, Fukatsu K, Johnson C, Kudsk K. The neuropeptide bombesin improves IgA-mediated mucosal immunity with preservation of gut interleukin-4 in total parenteral nutrition-fed mice. Surgery. 2002;131:59-65 pubmed
	Boneberg E, Hartung T. Mistletoe lectin-1 increases tumor necrosis factor-alpha release in lipopolysaccharide-stimulated whole blood via inhibition of interleukin-10 production. J Pharmacol Exp Ther. 2001;298:996-1000 pubmed
	Whittaker D, Bahjat K, Moldawer L, Clare Salzler M. Autoregulation of human monocyte-derived dendritic cell maturation and IL-12 production by cyclooxygenase-2-mediated prostanoid production. J Immunol. 2000;165:4298-304 pubmed
	Chung S, Yoshida E, Cattral M, Hu Y, Gorczynski R. Donor-specific stimulation of peripheral blood mononuclear cells from recipients of orthotopic liver transplants is associated, in the absence of rejection, with type-2 cytokine production. Immunol Lett. 1998;63:91-6 pubmed
	Anstey N, Weinberg J, Hassanali M, Mwaikambo E, Manyenga D, Misukonis M, et al. Nitric oxide in Tanzanian children with malaria: inverse relationship between malaria severity and nitric oxide production/nitric oxide synthase type 2 expression. J Exp Med. 1996;184:557-67 pubmed
	Chomarat P, Rissoan M, Pin J, Banchereau J, Miossec P. Contribution of IL-1, CD14, and CD13 in the increased IL-6 production induced by in vitro monocyte-synoviocyte interactions. J Immunol. 1995;155:3645-52 pubmed

product information

Product Type :

Antibody

Product Name :

IL-10 Monoclonal Antibody (12G8), Biotin

Catalog # :

M011B

Quantity :

250 µg

Price :

US 430.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Rat

Reactivity :

Human

Applications :

ELISA: Assay-dependent, Western Blot: 1:1000

Species :

Human

Clone :

12G8

Isotype :

IgG2a

Storage :

-20°C

Description :

IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.

Immunogen :

Recombinant human IL-10 (COS cell-derived)

Format :

Liquid

Applications w/Dilutions :

ELISA: Assay-dependent, Western Blot: 1:1000

Aliases :

CSIF; cytokine; Cytokine synthesis inhibitory factor; GVHDS; H-IL-10; il 10; IL10; IL-10; IL-10 precursor; IL10A; IL10X; ILN; Interleukin; interleukin 10; Interleukin10; interleukin-10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor; TGIF

more info or order :

Invitrogen product webpage