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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
CD11b Monoclonal Antibody (VIM12)
catalog :
CD11B00
quantity :
500 µL
price :
US 220
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
VIM12
reactivity :
human, mouse
application :
immunoprecipitation, flow cytometry, blocking or activating experiments
more info or order :
Invitrogen product webpage
citations: 12
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...
Okoye Okafor U, Bartholdy B, Cartier J, Gao E, Pietrak B, Rendina A, et al. New IDH1 mutant inhibitors for treatment of acute myeloid leukemia. Nat Chem Biol. 2015;11:878-86 pubmed publisher
  • flow cytometry; human; loading ...; fig 2a
Curtis B, Roman A, Sullivan M, Raven C, Larison J, Weitekamp L. Two cases of maternal alloimmunization against human neutrophil alloantigen-4b, one causing severe alloimmune neonatal neutropenia. Transfusion. 2016;56:101-6 pubmed publisher
  • blocking or activating experiments; human; fig 7
Nakayama H, Yoshizaki F, Prinetti A, Sonnino S, Mauri L, Takamori K, et al. Lyn-coupled LacCer-enriched lipid rafts are required for CD11b/CD18-mediated neutrophil phagocytosis of nonopsonized microorganisms. J Leukoc Biol. 2008;83:728-41 pubmed
  • flow cytometry; human; tbl 2
Bratke K, Kuepper M, Bade B, Virchow J, Luttmann W. Differential expression of human granzymes A, B, and K in natural killer cells and during CD8+ T cell differentiation in peripheral blood. Eur J Immunol. 2005;35:2608-16 pubmed
  • flow cytometry; human
Mathison R, Befus A, Davison J, Woodman R. Modulation of neutrophil function by the tripeptide feG. BMC Immunol. 2003;4:3 pubmed
  • flow cytometry; human; tbl 1
  • immunoprecipitation; human; fig 2
Diamond M, Garcia Aguilar J, Bickford J, Corbi A, Springer T. The I domain is a major recognition site on the leukocyte integrin Mac-1 (CD11b/CD18) for four distinct adhesion ligands. J Cell Biol. 1993;120:1031-43 pubmed
  • flow cytometry; human; fig 9
Bohuslav J, Horejsi V, Hansmann C, Stockl J, Weidle U, Majdic O, et al. Urokinase plasminogen activator receptor, beta 2-integrins, and Src-kinases within a single receptor complex of human monocytes. J Exp Med. 1995;181:1381-90 pubmed
Han X, Su X, Li Z, Liu Y, Wang S, Zhu M, et al. Complement receptor 3 mediates Aspergillus fumigatus internalization into alveolar epithelial cells with the increase of intracellular phosphatidic acid by activating FAK. Virulence. 2021;12:1980-1996 pubmed publisher
Camilli G, Eren E, Williams D, Aimanianda V, Meunier E, Quintin J. Impaired phagocytosis directs human monocyte activation in response to fungal derived β-glucan particles. Eur J Immunol. 2018;48:757-770 pubmed publisher
Bzowska M, Nogieć A, Bania K, Zygmunt M, Zarebski M, Dobrucki J, et al. Involvement of cell surface 90 kDa heat shock protein (HSP90) in pattern recognition by human monocyte-derived macrophages. J Leukoc Biol. 2017;102:763-774 pubmed publisher
Linden J, Kunkel D, Laforce Nesbitt S, Bliss J. The role of galectin-3 in phagocytosis of Candida albicans and Candida parapsilosis by human neutrophils. Cell Microbiol. 2013;15:1127-42 pubmed publisher
Whitlock B, Gardai S, Fadok V, Bratton D, Henson P. Differential roles for alpha(M)beta(2) integrin clustering or activation in the control of apoptosis via regulation of akt and ERK survival mechanisms. J Cell Biol. 2000;151:1305-20 pubmed
product information
Product Type :
Antibody
Product Name :
CD11b Monoclonal Antibody (VIM12)
Catalog # :
CD11b00
Quantity :
500 µL
Price :
US 220
Clonality :
Monoclonal
Purity :
purified
Host :
Mouse
Reactivity :
Human
Applications :
Flow Cytometry: Assay-Dependent
Species :
Human
Clone :
VIM12
Isotype :
IgG1
Storage :
4° C, store in dark
Description :
CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3). CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain. CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions. CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Further, peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus.
Immunogen :
Human CD11b (Mac-1)
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: Assay-Dependent
Aliases :
antigen CD11b (p170); antigen CD11b (p170); macrophage antigen alpha polypeptide; CD11 antigen-like family member B; CD11b; CD11B (p170); CD11b/CD18; cell surface glycoprotein MAC-1 alpha subunit; cell surface glycoprotein MAC-1 subunit alpha; complement component 3 receptor 3 subunit; complement component receptor 3 alpha-a; complement receptor type 3; CR3; CR-3 alpha chain; CR3A; F730045J24Rik; integrin alpha M; integrin alpha-M; integrin subunit alpha M; integrin, alpha M; integrin, alpha M (complement component 3 receptor 3 subunit); ITGAM; Leukocyte adhesion receptor MO1; leukocyte integrin alpha-M chain; LOC100351865; Ly-40; MAC1; Mac-1; Mac-1 alpha; Mac-1 alpha (Mac1A); MAC-1 alpha subunit; MAC1A; Mac-1a; macrophage antigen alpha; macrophage antigen alpha polypeptide; MGC117044; MO1A; Neutrophil adherence receptor; neutrophil adherence receptor alpha-M subunit; SLEB6; unnamed protein product
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA