IFN gamma Monoclonal Antibody (DB-1 (DB1)) | Invitrogen ARC4033 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

IFN gamma Monoclonal Antibody (DB-1 (DB1))

catalog :

ARC4033

quantity :

1 mg

price :

399.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

DB-1 (DB1)

reactivity :

mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, neutralization, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, blocking or activating experiments

citations: 29

Published Application/Species/Sample/Dilution	Reference
flow cytometry; rat; fig s6e	Flach A, Litke T, Strauss J, Haberl M, GÃ³mez C, Reindl M, et al. Autoantibody-boosted T-cell reactivation in the target organ triggers manifestation of autoimmune CNS disease. Proc Natl Acad Sci U S A. 2016;113:3323-8 pubmed publisher
ELISA; rat; loading ...; fig 4b	Nacka AleksiÄ‡ M, DjikiÄ‡ J, PilipoviÄ‡ I, StojiÄ‡ VukaniÄ‡ Z, Kosec D, Bufan B, et al. Male rats develop more severe experimental autoimmune encephalomyelitis than female rats: sexual dimorphism and diergism at the spinal cord level. Brain Behav Immun. 2015;49:101-18 pubmed publisher
immunohistochemistry; rat	Pusic A, Mitchell H, Kunkler P, Klauer N, Kraig R. Spreading depression transiently disrupts myelin via interferon-gamma signaling. Exp Neurol. 2015;264:43-54 pubmed publisher
ELISA; rat; fig 5	Hoentjen F, Tonkonogy S, Liu B, Sartor R, Taurog J, Dieleman L. Adoptive transfer of nontransgenic mesenteric lymph node cells induces colitis in athymic HLA-B27 transgenic nude rats. Clin Exp Immunol. 2006;143:474-83 pubmed
ELISA; mouse; fig 4	Al Qaoud K, Abdel Hafez S. Humoral and cytokine response during protection of mice against secondary hydatidosis caused by Echinococcus granulosus. Parasitol Res. 2005;98:54-60 pubmed
ELISA; rat; fig 5	Hoentjen F, Welling G, Harmsen H, Zhang X, Snart J, Tannock G, et al. Reduction of colitis by prebiotics in HLA-B27 transgenic rats is associated with microflora changes and immunomodulation. Inflamm Bowel Dis. 2005;11:977-85 pubmed
ELISA; rat	Qian B, Tonkonogy S, Hoentjen F, Dieleman L, Sartor R. Dysregulated luminal bacterial antigen-specific T-cell responses and antigen-presenting cell function in HLA-B27 transgenic rats with chronic colitis. Immunology. 2005;116:112-21 pubmed
ELISA; rat; 1 ug/ml	Janelidze S, Enell K, Visse E, Darabi A, Salford L, Siesjo P. Activation of purified allogeneic CD4(+) T cells by rat bone marrow-derived dendritic cells induces concurrent secretion of IFN-gamma, IL-4, and IL-10. Immunol Lett. 2005;101:193-201 pubmed
ELISA; rat; 10 ug/ml; fig 4	Moalem G, Xu K, Yu L. T lymphocytes play a role in neuropathic pain following peripheral nerve injury in rats. Neuroscience. 2004;129:767-77 pubmed
ELISA; rat; fig 3	Todd D, Forsberg E, Greiner D, Mordes J, Rossini A, Bortell R. Deficiencies in gut NK cell number and function precede diabetes onset in BB rats. J Immunol. 2004;172:5356-62 pubmed
ELISA; mouse	Cabarrocas J, Piaggio E, Zappulla J, Desbois S, Mars L, Lassmann H, et al. A transgenic mouse model for T-cell ignorance of a glial autoantigen. J Autoimmun. 2004;22:179-89 pubmed
ELISA; rat; fig 3	Dieleman L, Hoentjen F, Qian B, Sprengers D, Tjwa E, Torres M, et al. Reduced ratio of protective versus proinflammatory cytokine responses to commensal bacteria in HLA-B27 transgenic rats. Clin Exp Immunol. 2004;136:30-9 pubmed
blocking or activating experiments; rat	Chakir H, Wang H, Lefebvre D, Webb J, Scott F. T-bet/GATA-3 ratio as a measure of the Th1/Th2 cytokine profile in mixed cell populations: predominant role of GATA-3. J Immunol Methods. 2003;278:157-69 pubmed
immunohistochemistry - frozen section; mouse	Archin N, Atherton S. Infiltration of T-lymphocytes in the brain after anterior chamber inoculation of a neurovirulent and neuroinvasive strain of HSV-1. J Neuroimmunol. 2002;130:117-27 pubmed
blocking or activating experiments; rat	Sorkness R, Castleman W, Mikus L, Mosser A, Lemanske R. Chronic postbronchiolitis airway instability induced with anti-IFN-gamma antibody in F344 rats. Pediatr Res. 2002;52:382-6 pubmed
ELISA; rat; 10 ug/ml; fig 2	Hylkema M, Timens W, Luinge M, Van Der Werf N, Hoekstra M. The effect of bacillus Calmette-Guérin immunization depends on the genetic predisposition to Th2-type responsiveness. Am J Respir Cell Mol Biol. 2002;27:244-9 pubmed
flow cytometry; rat	Uehara K, Ichida T, Sugahara S, Ishikawa T, Yamagiwa S, Yoshida Y, et al. Systemic administration of liposome-encapsulated OK-432 prolongs the survival of rats with hepatocellular carcinoma through the induction of IFN-gamma-producing hepatic lymphocytes. J Gastroenterol Hepatol. 2002;17:81-90 pubmed
ELISA; rat; fig 8	Todd D, Greiner D, Rossini A, Mordes J, Bortell R. An atypical population of NK cells that spontaneously secrete IFN-gamma and IL-4 is present in the intraepithelial lymphoid compartment of the rat. J Immunol. 2001;167:3600-9 pubmed
ELISA; mouse; fig 1	Liva S, Voskuhl R. Testosterone acts directly on CD4+ T lymphocytes to increase IL-10 production. J Immunol. 2001;167:2060-7 pubmed
flow cytometry; rat	Caraher E, Parenteau M, Gruber H, Scott F. Flow cytometric analysis of intracellular IFN-gamma, IL-4 and IL-10 in CD3(+)4(+) T-cells from rat spleen. J Immunol Methods. 2000;244:29-40 pubmed
immunohistochemistry - paraffin section; rat; fig 6	Lin Y, Soares M, Sato K, Csizmadia E, Robson S, Smith N, et al. Long-term survival of hamster hearts in presensitized rats. J Immunol. 2000;164:4883-92 pubmed
ELISA; rat; 2 ug/ml; fig 1	Kawai T, Seki M, Hiromatsu K, Eastcott J, Watts G, Sugai M, et al. Selective diapedesis of Th1 cells induced by endothelial cell RANTES. J Immunol. 1999;163:3269-78 pubmed
ELISA; rat	Cetre C, Pierrot C, Cocude C, Lafitte S, Capron A, Capron M, et al. Profiles of Th1 and Th2 cytokines after primary and secondary infection by Schistosoma mansoni in the semipermissive rat host. Infect Immun. 1999;67:2713-9 pubmed
immunohistochemistry - paraffin section; rat	Lin Y, Soares M, Sato K, Takigami K, Csizmadia E, Anrather J, et al. Rejection of cardiac xenografts by CD4+ or CD8+ T cells. J Immunol. 1999;162:1206-14 pubmed
immunohistochemistry; rat	Miyatake T, Koyamada N, Hancock W, Soares M, Bach F. Survival of accommodated cardiac xenografts upon retransplantation into cyclosporine-treated recipients. Transplantation. 1998;65:1563-9 pubmed
ELISA; mouse; fig 1	Shibata Y, Metzger W, Myrvik Q. Chitin particle-induced cell-mediated immunity is inhibited by soluble mannan: mannose receptor-mediated phagocytosis initiates IL-12 production. J Immunol. 1997;159:2462-7 pubmed
ELISA; mouse	Shibata Y, Foster L, Metzger W, Myrvik Q. Alveolar macrophage priming by intravenous administration of chitin particles, polymers of N-acetyl-D-glucosamine, in mice. Infect Immun. 1997;65:1734-41 pubmed
blocking or activating experiments; mouse	Früh R, Blum B, Mossmann H, Domdey H, von Specht B. TH1 cells trigger tumor necrosis factor alpha-mediated hypersensitivity to Pseudomonas aeruginosa after adoptive transfer into SCID mice. Infect Immun. 1995;63:1107-12 pubmed
	Kortagere S, Xu J, Mankowski M, Ptak R, Cocklin S. Structure-activity relationships of a novel capsid targeted inhibitor of HIV-1 replication. J Chem Inf Model. 2014;54:3080-90 pubmed publisher

product information

Product Type :

Antibody

Product Name :

IFN gamma Monoclonal Antibody (DB-1 (DB1))

Catalog # :

ARC4033

Quantity :

1 mg

Price (USD) :

399.00 USD

Clonality :

Monoclonal

Host :

Mouse

Reactivity :

Mouse, Rat

Applications :

ELISA: 10 µg/mL, Immunocytochemistry: Assay Dependent, Immunohistochemistry (Frozen): Assay Dependent, Neutralization: Assay Dependent, Western Blot: Assay Dependent

Species :

Mouse, Rat

Clone :

DB-1 (DB1)

Isotype :

IgG1, kappa

Storage :

4° C

Description :

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.

Immunogen :

Rat/Mouse IFN-gamma.

Format :

Lyophilized

Applications w/Dilutions :

ELISA: 10 µg/mL, Immunocytochemistry: Assay Dependent, Immunohistochemistry (Frozen): Assay Dependent, Neutralization: Assay Dependent, Western Blot: Assay Dependent

Aliases :

BoIFNG; cytokine; EGK_03901; gamma interferon; gamma interferon precursor; gamma-interferon; gamma-interferon precursor (AA -23 to 143); H-IFN-g; Ifg; IFI; IFN gamma; IFN ?; IFNG; IFN-g; IFNG2; IFN-gamma; IFN-gamma precursor; IFN-y; IFN?; Immune interferon; interferon gamma; interferon gamma precursor; interferon gamma type 2; Interferon y; Interferon ?; interferon, gamma; interferon-gama; interferon-gamma; Interferon-gamma level; interferon-gamma precursor; Interferon?; M-IFN-g; R-IFN-g