p27 Kip1 Monoclonal Antibody (DCS-72.F6) | Invitrogen AHZ0452 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

p27 Kip1 Monoclonal Antibody (DCS-72.F6)

catalog :

AHZ0452

quantity :

100 µg

price :

US 436

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

DCS-72.F6

reactivity :

human, mouse, rat, dogs

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

citations: 15

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; 1:100; fig 5b	Fang J, Coon B, Gillis N, Chen Z, Qiu J, Chittenden T, et al. Shear-induced Notch-Cx37-p27 axis arrests endothelial cell cycle to enable arterial specification. Nat Commun. 2017;8:2149 pubmed publisher
western blot; human; 1:500; fig 2	Martínez M, Ubeda A, Moreno J, Trillo M. Power Frequency Magnetic Fields Affect the p38 MAPK-Mediated Regulation of NB69 Cell Proliferation Implication of Free Radicals. Int J Mol Sci. 2016;17:510 pubmed publisher
immunocytochemistry; mouse; 1:200; fig 5	Yoshikawa M, Ouji Y. Induction of Inner Ear Hair Cells from Mouse Embryonic Stem Cells In Vitro. Methods Mol Biol. 2016;1516:257-267 pubmed publisher
immunohistochemistry; mouse; 1:200	Golden E, Benito Gonzalez A, Doetzlhofer A. The RNA-binding protein LIN28B regulates developmental timing in the mammalian cochlea. Proc Natl Acad Sci U S A. 2015;112:E3864-73 pubmed publisher
western blot; human; 1:200	Benzina S, Pitaval A, Lemercier C, Lustremant C, Frouin V, Wu N, et al. A kinome-targeted RNAi-based screen links FGF signaling to H2AX phosphorylation in response to radiation. Cell Mol Life Sci. 2015;72:3559-73 pubmed publisher
western blot; mouse; fig 1	Chen S, Fu S, Hsu S, Huang Y, Hsu B. Synergistic Effect of Hyperglycemia and p27(kip1) Suppression on Adult Mouse Islet Beta Cell Replication. Int J Endocrinol. 2012;2012:417390 pubmed publisher
immunohistochemistry - paraffin section; human; 1:40	Jung Y, Joo K, Seong D, Choi Y, Kong D, Kim Y, et al. Identification of prognostic biomarkers for glioblastomas using protein expression profiling. Int J Oncol. 2012;40:1122-32 pubmed publisher
immunohistochemistry - paraffin section; mouse; fig 2	Hagihara K, Zhang E, Ke Y, Liu G, Liu J, Rao Y, et al. Shp2 acts downstream of SDF-1alpha/CXCR4 in guiding granule cell migration during cerebellar development. Dev Biol. 2009;334:276-84 pubmed publisher
western blot; human; fig 7	Seminario M, Precht P, Wersto R, Gorospe M, Wange R. PTEN expression in PTEN-null leukaemic T cell lines leads to reduced proliferation via slowed cell cycle progression. Oncogene. 2003;22:8195-204 pubmed
	Hernández Bule M, Medel E, Colastra C, Roldan R, Ubeda A. Response of neuroblastoma cells to RF currents as a function of the signal frequency. BMC Cancer. 2019;19:889 pubmed publisher
	Gierisch M, Pedot G, Walser F, Lopez Garcia L, Jaaks P, Niggli F, et al. USP19 deubiquitinates EWS-FLI1 to regulate Ewing sarcoma growth. Sci Rep. 2019;9:951 pubmed publisher
	Thambiah L, Bindushree R, Anjum A, Pugazhendi S, Babu L, Nair R. Evaluating the expression of p16 and p27 in oral epithelial dysplasias and oral squamous cell carcinoma: A diagnostic marker for carcinogenesis. J Oral Maxillofac Pathol. 2018;22:59-64 pubmed publisher
	de Melo J, Miki K, Rattner A, Smallwood P, Zibetti C, Hirokawa K, et al. Injury-independent induction of reactive gliosis in retina by loss of function of the LIM homeodomain transcription factor Lhx2. Proc Natl Acad Sci U S A. 2012;109:4657-62 pubmed publisher
	Fernandez Marcos P, Pantoja C, Gonzalez Rodriguez A, Martin N, Flores J, Valverde A, et al. Normal proliferation and tumorigenesis but impaired pancreatic function in mice lacking the cell cycle regulator sei1. PLoS ONE. 2010;5:e8744 pubmed publisher
	James M, Ray A, Leznova D, Blain S. Differential modification of p27Kip1 controls its cyclin D-cdk4 inhibitory activity. Mol Cell Biol. 2008;28:498-510 pubmed

product information

Product Type :

Antibody

Product Name :

p27 Kip1 Monoclonal Antibody (DCS-72.F6)

Catalog # :

AHZ0452

Quantity :

100 µg

Price :

US 436

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Canine, Human, Mouse, Rat

Applications :

Flow Cytometry: Assay-Dependent, Immunofluorescence: Assay Dependent, Immunohistochemistry: Assay Dependent, Immunoprecipitation: Assay-Dependent, Western Blot: Assay-Dependent

Species :

Canine, Human, Mouse, Rat

Clone :

DCS-72.F6

Isotype :

IgG1, kappa

Storage :

4° C

Description :

p27 Kip1 functions as a negative regulator of G1 mouse expression and has been proposed to function as a possible mediator of TGFb induced G1 arrest. p27 Kip1 is an important regulator of cell cycle progression, a potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. p27 Kip1 forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. p27 Kip1 acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry. p27 Kip1 has a molecular weight of approximately 27kDa and the degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.

Immunogen :

Mouse recombinant p27Kip1 protein.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: Assay-Dependent, Immunofluorescence: Assay Dependent, Immunohistochemistry: Assay Dependent, Immunoprecipitation: Assay-Dependent, Western Blot: Assay-Dependent

Aliases :

AA408329; AI843786; Cdki1b; Cdkn1b; CDKN4; CDN1B; cyclin dependent kinase inhibitor 1B; cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor 1B (p27 Kip1); cyclin-dependent kinase inhibitor 1B (p27, Kip1); Cyclin-dependent kinase inhibitor p27; cyclin-dependent kinase inhibitor p27/Kip1; KIP1; MEN1B; MEN4; p27; P27 CDKN1B; p27(KIP1); P27KIP1; putative cyclin-dependent kinase inhibitor p27