CD9 Monoclonal Antibody (MM2/57) | Invitrogen AHS0902 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CD9 Monoclonal Antibody (MM2/57)

catalog :

AHS0902

quantity :

100 µg

price :

US 286.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

MM2/57

reactivity :

hamsters, human, mouse, pigs

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

more info or order :

Invitrogen product webpage

citations: 23

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:1000; loading ...; fig 1c	Koba T, Takeda Y, Narumi R, Shiromizu T, Nojima Y, Ito M, et al. Proteomics of serum extracellular vesicles identifies a novel COPD biomarker, fibulin-3 from elastic fibres. ERJ Open Res. 2021;7: pubmed publisher
immunocytochemistry; pigs ; 1:200; loading ...; tbl 5	DÃ©moulins T, Englezou P, Milona P, Ruggli N, Tirelli N, Pichon C, et al. Self-Replicating RNA Vaccine Delivery to Dendritic Cells. Methods Mol Biol. 2017;1499:37-75 pubmed
flow cytometry; human; fig s1	Zhou P, Erfani S, Liu Z, Jia C, Chen Y, Xu B, et al. CD151-Î±3Î²1 integrin complexes are prognostic markers of glioblastoma and cooperate with EGFR to drive tumor cell motility and invasion. Oncotarget. 2015;6:29675-93 pubmed publisher
western blot; mouse; fig 1	Jin Y, Tachibana I, Takeda Y, He P, Kang S, Suzuki M, et al. Statins decrease lung inflammation in mice by upregulating tetraspanin CD9 in macrophages. PLoS ONE. 2013;8:e73706 pubmed publisher
flow cytometry; human; fig 6 western blot; human; fig 6	Kohmo S, Kijima T, Otani Y, Mori M, Minami T, Takahashi R, et al. Cell surface tetraspanin CD9 mediates chemoresistance in small cell lung cancer. Cancer Res. 2010;70:8025-35 pubmed publisher
western blot; human; fig 2	Castro Sanchez L, Soto Guzman A, Navarro Tito N, Martinez Orozco R, Salazar E. Native type IV collagen induces cell migration through a CD9 and DDR1-dependent pathway in MDA-MB-231 breast cancer cells. Eur J Cell Biol. 2010;89:843-52 pubmed publisher
immunocytochemistry; human; fig 2 western blot; human; fig 5	Lafleur M, Xu D, Hemler M. Tetraspanin proteins regulate membrane type-1 matrix metalloproteinase-dependent pericellular proteolysis. Mol Biol Cell. 2009;20:2030-40 pubmed publisher
western blot; human; fig 1	Takeda Y, He P, Tachibana I, Zhou B, Miyado K, Kaneko H, et al. Double deficiency of tetraspanins CD9 and CD81 alters cell motility and protease production of macrophages and causes chronic obstructive pulmonary disease-like phenotype in mice. J Biol Chem. 2008;283:26089-97 pubmed publisher
immunocytochemistry; human western blot; human; fig 2	Yang X, Richardson A, Torres Arzayus M, Zhou P, Sharma C, Kazarov A, et al. CD151 accelerates breast cancer by regulating alpha 6 integrin function, signaling, and molecular organization. Cancer Res. 2008;68:3204-13 pubmed publisher
flow cytometry; human; fig 2A immunoprecipitation; human; fig 2B western blot; human; fig 2B	Saito Y, Tachibana I, Takeda Y, Yamane H, He P, Suzuki M, et al. Absence of CD9 enhances adhesion-dependent morphologic differentiation, survival, and matrix metalloproteinase-2 production in small cell lung cancer cells. Cancer Res. 2006;66:9557-65 pubmed
immunoprecipitation; hamsters; fig 5	Lanza F, Wolf D, Fox C, Kieffer N, Seyer J, Fried V, et al. cDNA cloning and expression of platelet p24/CD9. Evidence for a new family of multiple membrane-spanning proteins. J Biol Chem. 1991;266:10638-45 pubmed
	Konaka H, Kato Y, Hirano T, Tsujimoto K, Park J, Koba T, et al. Secretion of mitochondrial DNA via exosomes promotes inflammation in Behçet's syndrome. EMBO J. 2023;42:e112573 pubmed publisher
	Liu L, Kawashima M, Sugimoto M, Sonomura K, Pu F, Li W, et al. Discovery of lipid profiles in plasma-derived extracellular vesicles as biomarkers for breast cancer diagnosis. Cancer Sci. 2023;114:4020-4031 pubmed publisher
	Kawasaki T, Takeda Y, Edahiro R, Shirai Y, Nogami Itoh M, Matsuki T, et al. Next-generation proteomics of serum extracellular vesicles combined with single-cell RNA sequencing identifies MACROH2A1 associated with refractory COVID-19. Inflamm Regen. 2022;42:53 pubmed publisher
	Sch xfc rz M, Danmayr J, Jaritsch M, Klinglmayr E, Benirschke H, Matea C, et al. EVAnalyzer: High content imaging for rigorous characterisation of single extracellular vesicles using standard laboratory equipment and a new open-source ImageJ/Fiji plugin. J Extracell Vesicles. 2022;11:e12282 pubmed publisher
	Zarubova J, Hasani Sadrabadi M, Norris S, Majedi F, Xiao C, Kasko A, et al. Cell-Taxi: Mesenchymal Cells Carry and Transport Clusters of Cancer Cells. Small. 2022;18:e2203515 pubmed publisher
	Jiao W, Hao J, Xie Y, Meng M, Gao W. EZH2 mitigates the cardioprotective effects of mesenchymal stem cell-secreted exosomes against infarction via HMGA2-mediated PI3K/AKT signaling. BMC Cardiovasc Disord. 2022;22:95 pubmed publisher
	Qu X, Leung T, Ngai S, Tsai S, Thakur A, Li W, et al. Proteomic Analysis of Circulating Extracellular Vesicles Identifies Potential Biomarkers for Lymph Node Metastasis in Oral Tongue Squamous Cell Carcinoma. Cells. 2021;10: pubmed publisher
	Ma x142 ys M, Aigner C, Schulz S, Schachner H, Rees A, Kain R. Isolation of Small Extracellular Vesicles from Human Sera. Int J Mol Sci. 2021;22: pubmed publisher
	Grzesiak M, Popiolek K, Knapczyk Stwora K. Extracellular vesicles in follicular fluid of sexually mature gilts' ovarian antral follicles - identification and proteomic analysis. J Physiol Pharmacol. 2020;71: pubmed publisher
	Jin Y, Takeda Y, Kondo Y, Tripathi L, Kang S, Takeshita H, et al. Double deletion of tetraspanins CD9 and CD81 in mice leads to a syndrome resembling accelerated aging. Sci Rep. 2018;8:5145 pubmed publisher
	Israels S, McMillan Ward E. CD63 modulates spreading and tyrosine phosphorylation of platelets on immobilized fibrinogen. Thromb Haemost. 2005;93:311-8 pubmed
	Takeda Y, Tachibana I, Miyado K, Kobayashi M, Miyazaki T, Funakoshi T, et al. Tetraspanins CD9 and CD81 function to prevent the fusion of mononuclear phagocytes. J Cell Biol. 2003;161:945-56 pubmed

product information

Product Type :

Antibody

Product Name :

CD9 Monoclonal Antibody (MM2/57)

Catalog # :

AHS0902

Quantity :

100 µg

Price :

US 286.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Human

Applications :

Flow Cytometry: Assay-dependent, Immunohistochemistry: Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: Assay-dependent

Species :

Human

Clone :

MM2/57

Isotype :

IgG2b, kappa

Storage :

4° C

Description :

CD9 antigen is a glycoprotein expressed on the surface of developing B lymphocytes, platelets, monocytes, eosinophils, basophil, stimulated T lymphocytes and by neurons and glial cells in the peripheral nervous system. CD9 belongs to a family of membrane proteins termed tetraspanins which transverse the membrane four times. In pre B cells and platelets, CD9 antigen regulates cell activation and aggregation possibly through an association with the integrin CD41 / CD61 (GPIIb / GPIIIa). CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferation and is necessary for association of heterologous MHC II molecules on the dendritic cell plasma membrane which is important for effective T cell stimulation. CD9 functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression plays a critical role in the suppression of cancer cell motility and metastasis. CD9 is also considered as metastasis suppressor in solid tumors.

Immunogen :

Human platelet glycoprotein

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: Assay-dependent, Immunohistochemistry: Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: Assay-dependent

Aliases :

5H9; 5H9 antigen; antigen CD9; BA2; BA-2/p24 antigen; BTCC-1; CD9; CD9 antigen; CD9 antigen (p24); CD9 molecule; cell growth-inhibiting gene 2 protein; DRAP-27; GIG2; Leukocyte antigen MIC3; MIC3; motility related protein-1; Motility-related protein; MRP-1; p24; sCD 9; sCD9; soluble CD 9; soluble CD9; Tetraspanin29; Tetraspanin-29; transmembrane protein CD9; TSPAN29; Tspan-29

more info or order :

Invitrogen product webpage