Aggrecan Monoclonal Antibody (969D4D11) | Invitrogen AHP0022 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Aggrecan Monoclonal Antibody (969D4D11)

catalog :

AHP0022

quantity :

500 µL

price :

US 446.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

969D4D11

reactivity :

human, domestic sheep

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, blocking or activating experiments

more info or order :

Invitrogen product webpage

citations: 39

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; human; 1:50; loading ...; fig 3p	Akbari P, Waldman S, Propst E, Cushing S, Weber J, Yeger H, et al. Generating Mechanically Stable, Pediatric, and Scaffold-Free Nasal Cartilage Constructs In Vitro. Tissue Eng Part C Methods. 2016;22:1077-1084 pubmed
immunohistochemistry - frozen section; human; 1:500; loading ...; fig 2c	Bianchi V, Weber J, Waldman S, Backstein D, Kandel R. Formation of Hyaline Cartilage Tissue by Passaged Human Osteoarthritic Chondrocytes. Tissue Eng Part A. 2017;23:156-165 pubmed publisher
blocking or activating experiments; human	Larkin J, Lohr T, Elefante L, Shearin J, Matico R, Su J, et al. Translational development of an ADAMTS-5 antibody for osteoarthritis disease modification. Osteoarthritis Cartilage. 2015;23:1254-66 pubmed publisher
immunocytochemistry; human	Ã…hlÃ©n M, Roshani L, LidÃ©n M, Struglics A, RostgÃ¥rd Christensen L, Kartus J. Inflammatory cytokines and biomarkers of cartilage metabolism 8 years after anterior cruciate ligament reconstruction: results from operated and contralateral knees. Am J Sports Med. 2015;43:1460-6 pubmed publisher
immunohistochemistry - paraffin section; human; fig 9	Antunes J, Tsaryk R, Gonçalves R, Pereira C, Landes C, Brochhausen C, et al. Poly(γ-Glutamic Acid) as an Exogenous Promoter of Chondrogenic Differentiation of Human Mesenchymal Stem/Stromal Cells. Tissue Eng Part A. 2015;21:1869-85 pubmed publisher
immunocytochemistry; human; 1:300	Schrobback K, Klein T, Woodfield T. The importance of connexin hemichannels during chondroprogenitor cell differentiation in hydrogel versus microtissue culture models. Tissue Eng Part A. 2015;21:1785-94 pubmed publisher
immunohistochemistry - frozen section; human; 1:10,000	Bertolo A, Hafner S, Taddei A, Baur M, Pötzel T, Steffen F, et al. Injectable microcarriers as human mesenchymal stem cell support and their application for cartilage and degenerated intervertebral disc repair. Eur Cell Mater. 2015;29:70-80; discujssion 80-1 pubmed
immunohistochemistry - frozen section; human; 1:300	Levett P, Hutmacher D, Malda J, Klein T. Hyaluronic acid enhances the mechanical properties of tissue-engineered cartilage constructs. PLoS ONE. 2014;9:e113216 pubmed publisher
western blot; human; 1:500	Capossela S, Schlafli P, Bertolo A, Janner T, Stadler B, Pötzel T, et al. Degenerated human intervertebral discs contain autoantibodies against extracellular matrix proteins. Eur Cell Mater. 2014;27:251-63; discussion 263 pubmed
immunohistochemistry - paraffin section; human	Olderøy M, Lilledahl M, Beckwith M, Melvik J, Reinholt F, Sikorski P, et al. Biochemical and structural characterization of neocartilage formed by mesenchymal stem cells in alginate hydrogels. PLoS ONE. 2014;9:e91662 pubmed publisher
immunoprecipitation; human; fig 1	Larsson S, Lohmander L, Struglics A. An ARGS-aggrecan assay for analysis in blood and synovial fluid. Osteoarthritis Cartilage. 2014;22:242-9 pubmed publisher
immunohistochemistry - frozen section; human; fig 5	Bertolo A, Baur M, Aebli N, Ferguson S, Stoyanov J. Physiological testosterone levels enhance chondrogenic extracellular matrix synthesis by male intervertebral disc cells in vitro, but not by mesenchymal stem cells. Spine J. 2014;14:455-68 pubmed publisher
immunocytochemistry; human; fig 2	Fernandes A, Herlofsen S, Karlsen T, Küchler A, Fløisand Y, Brinchmann J. Similar properties of chondrocytes from osteoarthritis joints and mesenchymal stem cells from healthy donors for tissue engineering of articular cartilage. PLoS ONE. 2013;8:e62994 pubmed publisher
ELISA; human; fig 1	Swärd P, Frobell R, Englund M, Roos H, Struglics A. Cartilage and bone markers and inflammatory cytokines are increased in synovial fluid in the acute phase of knee injury (hemarthrosis)--a cross-sectional analysis. Osteoarthritis Cartilage. 2012;20:1302-8 pubmed publisher
immunohistochemistry - paraffin section; human; fig 1	Chuang C, Shahin K, Lord M, Melrose J, Doran P, Whitelock J. The cartilage matrix molecule components produced by human foetal cartilage rudiment cells within scaffolds and the role of exogenous growth factors. Biomaterials. 2012;33:4078-88 pubmed publisher
ELISA; human; fig 3	Larsson S, Englund M, Struglics A, Lohmander L. The association between changes in synovial fluid levels of ARGS-aggrecan fragments, progression of radiographic osteoarthritis and self-reported outcomes: a cohort study. Osteoarthritis Cartilage. 2012;20:388-95 pubmed publisher
ELISA; human; fig 4	Lord M, Estrella R, Chuang C, Youssef P, Karlsson N, Flannery C, et al. Not all lubricin isoforms are substituted with a glycosaminoglycan chain. Connect Tissue Res. 2012;53:132-41 pubmed publisher
western blot; human; 1:500; fig 2	Larsson S, Englund M, Struglics A, Lohmander L. Association between synovial fluid levels of aggrecan ARGS fragments and radiographic progression in knee osteoarthritis. Arthritis Res Ther. 2010;12:R230 pubmed publisher
ELISA; human; tbl 2	Catterall J, Stabler T, Flannery C, Kraus V. Changes in serum and synovial fluid biomarkers after acute injury (NCT00332254). Arthritis Res Ther. 2010;12:R229 pubmed publisher
immunohistochemistry; human; 1:20,000; fig 2	Smith S, Shu C, Melrose J. Comparative immunolocalisation of perlecan with collagen II and aggrecan in human foetal, newborn and adult ovine joint tissues demonstrates perlecan as an early developmental chondrogenic marker. Histochem Cell Biol. 2010;134:251-63 pubmed publisher
ELISA; human; 1:8000; fig 6	Swearingen C, Carpenter J, Siegel R, Brittain I, Dotzlaf J, Durham T, et al. Development of a novel clinical biomarker assay to detect and quantify aggrecanase-generated aggrecan fragments in human synovial fluid, serum and urine. Osteoarthritis Cartilage. 2010;18:1150-8 pubmed publisher
ELISA; human; fig 1	Swearingen C, Chambers M, Lin C, Marimuthu J, Rito C, Carter Q, et al. A short-term pharmacodynamic model for monitoring aggrecanase activity: injection of monosodium iodoacetate (MIA) in rats and assessment of aggrecan neoepitope release in synovial fluid using novel ELISAs. Osteoarthritis Cartilage. 2010;18:1159-66 pubmed publisher
immunohistochemistry - paraffin section; domestic sheep; 1:20,000	Melrose J, Smith S, Smith M, Little C. The use of Histochoice for histological examination of articular and growth plate cartilages, intervertebral disc and meniscus. Biotech Histochem. 2008;83:47-53 pubmed publisher
immunohistochemistry - frozen section; human; 2 ug/ml; fig 9	Gellynck K, Verdonk P, Van Nimmen E, Almqvist K, Gheysens T, Schoukens G, et al. Silkworm and spider silk scaffolds for chondrocyte support. J Mater Sci Mater Med. 2008;19:3399-409 pubmed publisher
immunohistochemistry; human; fig 1	Melrose J, Roughley P, Knox S, Smith S, Lord M, Whitelock J. The structure, location, and function of perlecan, a prominent pericellular proteoglycan of fetal, postnatal, and mature hyaline cartilages. J Biol Chem. 2006;281:36905-14 pubmed
immunohistochemistry - paraffin section; domestic sheep; 1:1000; fig 4	Melrose J, Smith S, Cake M, Read R, Whitelock J. Perlecan displays variable spatial and temporal immunolocalisation patterns in the articular and growth plate cartilages of the ovine stifle joint. Histochem Cell Biol. 2005;123:561-71 pubmed
flow cytometry; human	Verdonk P, Forsyth R, Wang J, Almqvist K, Verdonk R, Veys E, et al. Characterisation of human knee meniscus cell phenotype. Osteoarthritis Cartilage. 2005;13:548-60 pubmed
immunocytochemistry; human	Almqvist K, Wang L, Wang J, Baeten D, Cornelissen M, Verdonk R, et al. Culture of chondrocytes in alginate surrounded by fibrin gel: characteristics of the cells over a period of eight weeks. Ann Rheum Dis. 2001;60:781-90 pubmed
	Larsson S, Lohmander L, Struglics A. Biological variation of human aggrecan ARGS neoepitope in synovial fluid and serum in early-stage knee osteoarthritis and after knee injury. Osteoarthr Cartil Open. 2022;4:100307 pubmed publisher
	Wu M, Sermer C, Kandel R, Theodoropoulos J. Characterization of Migratory Cells From Bioengineered Bovine Cartilage in a 3D Co-culture Model. Am J Sports Med. 2022;50:3090-3101 pubmed publisher
	xd6 stlind E, Eek F, Stigmar K, Sant Anna A, Ekvall Hansson E, Struglics A. Associations Between Physical Activity, Self-reported Joint Function, and Molecular Biomarkers in Working Age Individuals With Hip and/or Knee Osteoarthritis. Clin Med Insights Arthritis Musculoskelet Disord. 2022;15:11795441221081063 pubmed publisher
	Deszcz I, Lis Nawara A, Grelewski P, Dragan S, Bar J. Utility of direct 3D co-culture model for chondrogenic differentiation of mesenchymal stem cells on hyaluronan scaffold (Hyaff-11). Regen Biomater. 2020;7:543-552 pubmed publisher
	Trevino R, Stoia J, Laurent M, Pacione C, Chubinskaya S, Wimmer M. ESTABLISHING A LIVE CARTILAGE-ON-CARTILAGE INTERFACE FOR TRIBOLOGICAL TESTING. Biotribology (Oxf). 2017;9:1-11 pubmed publisher
	Larsson S, Struglics A, Lohmander L, Frobell R. Surgical reconstruction of ruptured anterior cruciate ligament prolongs trauma-induced increase of inflammatory cytokines in synovial fluid: an exploratory analysis in the KANON trial. Osteoarthritis Cartilage. 2017;25:1443-1451 pubmed publisher
	Sermer C, Kandel R, ANDERSON J, Hurtig M, Theodoropoulos J. Platelet-rich plasma enhances the integration of bioengineered cartilage with native tissue in an in vitro model. J Tissue Eng Regen Med. 2018;12:427-436 pubmed publisher
	Roato I, Alotto D, Belisario D, Casarin S, Fumagalli M, Cambieri I, et al. Adipose Derived-Mesenchymal Stem Cells Viability and Differentiating Features for Orthopaedic Reparative Applications: Banking of Adipose Tissue. Stem Cells Int. 2016;2016:4968724 pubmed publisher
	Darwiche S, Scaletta C, Raffoul W, Pioletti D, Applegate L. Epiphyseal Chondroprogenitors Provide a Stable Cell Source for Cartilage Cell Therapy. Cell Med. 2012;4:23-32 pubmed publisher
	Tourville T, Poynter M, Desarno M, Struglics A, Beynnon B. Relationship between synovial fluid ARGS-aggrecan fragments, cytokines, MMPs, and TIMPs following acute ACL injury: A cross-sectional study. J Orthop Res. 2015;33:1796-803 pubmed publisher
	Bertolo A, Mehr M, Aebli N, Baur M, Ferguson S, Stoyanov J. Influence of different commercial scaffolds on the in vitro differentiation of human mesenchymal stem cells to nucleus pulposus-like cells. Eur Spine J. 2012;21 Suppl 6:S826-38 pubmed publisher

product information

Product Type :

Antibody

Product Name :

Aggrecan Monoclonal Antibody (969D4D11)

Catalog # :

AHP0022

Quantity :

500 µL

Price :

US 446.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Human

Applications :

ELISA: Assay-dependent, Immunohistochemistry: Assay-dependent, Western Blot: Assay-dependent

Species :

Human

Clone :

969D4D11

Isotype :

IgG1

Storage :

4° C

Description :

Aggrecan also known as cartilage-specific proteoglycan core protein (CSPCP) or chondroitin sulfate proteoglycan 1 is a protein that is encoded by the ACAN gene. The encoded protein is an integral part of the extracellular matrix in cartilaginous tissue and it withstands compression in cartilage. The human form of the protein is 2316 amino acids long (approximately 250 kDa) and can be expressed in multiple isoforms due to alternative splicing. Aggrecan exhibits a bottlebrush structure, in which chondroitin sulfate and keratan sulfate chains are attached to an extended protein core. The synthesis and degradation of aggrecan are being investigated for their roles in cartilage deterioration during joint injury, disease, and aging. It contains three interglobular domains (IGDs), G1, G2, and G3 that are involved in aggregation, hyaluronan binding, cell adhesion, and chondrocyte apoptosis. Aggrecan fragments from articular cartilage are released into the synovial fluid at all stages of human osteoarthritis as a result of cleavage by aggrecanase and MMP activity. Antibodies BC-3 and BC-4 can be used to detect these neo epitopes of Aggrecanase (ARGSVXXX) and MMP (XXXVDIPEN), respectively.

Immunogen :

Human natural aggrecan (proteoglycan), purified from human articular cartilage.

Format :

Liquid

Applications w/Dilutions :

ELISA: Assay-dependent, Immunohistochemistry: Assay-dependent, Western Blot: Assay-dependent

Aliases :

ACAN; Agc; Agc1; AGCAN; aggrecan; aggrecan 1; aggrecan 1 (chondroitin sulfate proteoglycan 1, large aggregating proteoglycan, antigen identified by monoclonal antibody A0122); aggrecan core protein; Aggrecan core protein 2; aggrecan CS2 domain; aggrecan epidermal growth factor-like domain-1; aggrecan G2 domain; aggrecan interglobular domain; aggrecan keratan sulfate domain; aggrecan, structural proteoglycan of cartilage; articular cartilage aggrecan precursor; b2b183Clo; cartilage aggregating proteoglycan; cartilage-specific proteoglycan core protein; Chondroitin sulfate proteoglycan 1; chondroitin sulfate proteoglycan core protein 1; cmd; CSPCP; CSPG1; CSPGCP; high density cartilage proteoglycan; large aggregating cartilage proteoglycan core protein; large aggregating proteoglycan; large aggregating proteoglycan, antigen; MSK16; SEDK

more info or order :

Invitrogen product webpage