PRAS40 Monoclonal Antibody (73P21) | Invitrogen AHO1031 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

PRAS40 Monoclonal Antibody (73P21)

catalog :

AHO1031

quantity :

100 µg

price :

US 430.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

73P21

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, other

more info or order :

Invitrogen product webpage

citations: 8

Published Application/Species/Sample/Dilution	Reference
other; human; loading ...; fig 4c	Ng P, Li J, Jeong K, Shao S, Chen H, Tsang Y, et al. Systematic Functional Annotation of Somatic Mutations in Cancer. Cancer Cell. 2018;33:450-462.e10 pubmed publisher
western blot; human; 1:1000	Webber P, Park C, Qui M, Ramalingam S, Khuri F, Fu H, et al. Combination of heat shock protein 90 and focal adhesion kinase inhibitors synergistically inhibits the growth of non-small cell lung cancer cells. Oncoscience. 2015;2:765-776 pubmed
western blot; human; 1:1000; fig 1	Yu P, Laird A, Du X, Wu J, Won K, Yamaguchi K, et al. Characterization of the activity of the PI3K/mTOR inhibitor XL765 (SAR245409) in tumor models with diverse genetic alterations affecting the PI3K pathway. Mol Cancer Ther. 2014;13:1078-91 pubmed publisher
western blot; human; fig 1B	Oshiro N, Takahashi R, Yoshino K, Tanimura K, Nakashima A, Eguchi S, et al. The proline-rich Akt substrate of 40 kDa (PRAS40) is a physiological substrate of mammalian target of rapamycin complex 1. J Biol Chem. 2007;282:20329-39 pubmed
	Zhou X, Zhong Y, Molinar Inglis O, Kunkel M, Chen M, Sun T, et al. Location-specific inhibition of Akt reveals regulation of mTORC1 activity in the nucleus. Nat Commun. 2020;11:6088 pubmed publisher
	Snel M, Gastaldelli A, Ouwens D, Hesselink M, Schaart G, Buzzigoli E, et al. Effects of adding exercise to a 16-week very low-calorie diet in obese, insulin-dependent type 2 diabetes mellitus patients. J Clin Endocrinol Metab. 2012;97:2512-20 pubmed publisher
	Nascimento E, Snel M, Guigas B, van der Zon G, Kriek J, Maassen J, et al. Phosphorylation of PRAS40 on Thr246 by PKB/AKT facilitates efficient phosphorylation of Ser183 by mTORC1. Cell Signal. 2010;22:961-7 pubmed publisher
	Vander Haar E, Lee S, Bandhakavi S, Griffin T, Kim D. Insulin signalling to mTOR mediated by the Akt/PKB substrate PRAS40. Nat Cell Biol. 2007;9:316-23 pubmed

product information

Product Type :

Antibody

Product Name :

PRAS40 Monoclonal Antibody (73P21)

Catalog # :

AHO1031

Quantity :

100 µg

Price :

US 430.00

Clonality :

Monoclonal

Purity :

purified

Host :

Mouse

Reactivity :

Human

Applications :

Immunocytochemistry: 1:100-1:500, Immunohistochemistry (Paraffin): 1:10-1:100, Immunoprecipitation: Assay-dependent, Western Blot: Assay-dependent

Species :

Human

Clone :

73P21

Isotype :

IgG1, kappa

Storage :

-20°C

Description :

The Akt signaling pathway contributes to the regulation of apoptosis after a variety of cell death signals. AKT1S1, also known as PRAS40, is a proline-rich substrate of the kinase AKT1 and is thought to play a role in neuroprotection mediated by nerve growth factor (NGF) after transient focal cerebral ischemia. AKT1S1 is also a substrate and potential regulator of mammalian target of rapamycin (mTOR), a serine/threonine kinase that regulates cell growth and cell cycle, and a negative regulator of autophagy. Treatment with the insulin-like growth factor-1 (IGF1) can induce the phosphorylation of AKT1S1 via the PI3K/AKT signaling pathway in PC12 cells.

Immunogen :

Recombinant full length human PRAS40 expressed in E. coli.

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1:100-1:500, Immunohistochemistry (Paraffin): 1:10-1:100, Immunoprecipitation: Assay-dependent, Western Blot: Assay-dependent

Aliases :

1110012J22Rik; 40 kDa proline-rich AKT substrate; AI227026; AKT1 substrate 1; AKT1 substrate 1 (proline rich); AKT1 substrate 1 (proline-rich); akt1s1; Lobe; Lobel; OTTHUMP00000196756; OTTHUMP00000196760; PKB/Akt substrate 40; Pras; PRAS40; Proline-rich AKT substrate; proline-rich AKT1 substrate 1

more info or order :

Invitrogen product webpage