EIF2S1 Monoclonal Antibody (EIF2-alpha) | Invitrogen AHO0802 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

EIF2S1 Monoclonal Antibody (EIF2-alpha)

catalog :

AHO0802

quantity :

100 µg

price :

US 450.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

EIF2-alpha

reactivity :

African green monkey, human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, RNA immunoprecipitation

more info or order :

Invitrogen product webpage

citations: 41

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:1000; loading ...; fig 4g	Guo X, Aviles G, Liu Y, Tian R, Unger B, Lin Y, et al. Mitochondrial stress is relayed to the cytosol by an OMA1-DELE1-HRI pathway. Nature. 2020;579:427-432 pubmed publisher
western blot; rat; 1:2000; loading ...; fig 7h	Santos M, Anderson C, Neschen S, Zumbrennen Bullough K, Romney S, Kahle Stephan M, et al. Irp2 regulates insulin production through iron-mediated Cdkal1-catalyzed tRNA modification. Nat Commun. 2020;11:296 pubmed publisher
western blot; human; fig s5a	Chung H, Calis J, Wu X, Sun T, Yu Y, Sarbanes S, et al. Human ADAR1 Prevents Endogenous RNA from Triggering Translational Shutdown. Cell. 2018;172:811-824.e14 pubmed publisher
western blot; mouse; 1:1000; loading ...; fig 1a	Silva R, Sattlegger E, Castilho B. Perturbations in actin dynamics reconfigure protein complexes that modulate GCN2 activity and promote an eIF2 response. J Cell Sci. 2016;129:4521-4533 pubmed
western blot; human; loading ...; fig 5	Mohammad S, Wolfe L, Stöbe P, Biskup S, Wainwright M, Melin Aldana H, et al. Infantile Cirrhosis, Growth Impairment, and Neurodevelopmental Anomalies Associated with Deficiency of PPP1R15B. J Pediatr. 2016;179:144-149.e2 pubmed publisher
western blot; mouse	Maas N, Singh N, Diehl J. Generation and characterization of an analog-sensitive PERK allele. Cancer Biol Ther. 2014;15:1106-11 pubmed publisher
western blot; mouse; 1:1000; fig 3	Hengstermann A, Muller T. Endoplasmic reticulum stress induced by aqueous extracts of cigarette smoke in 3T3 cells activates the unfolded-protein-response-dependent PERK pathway of cell survival. Free Radic Biol Med. 2008;44:1097-107 pubmed publisher
western blot; human	Shah A, Parker J, Gillespie G, Lakeman F, Meleth S, Markert J, et al. Enhanced antiglioma activity of chimeric HCMV/HSV-1 oncolytic viruses. Gene Ther. 2007;14:1045-54 pubmed
western blot; African green monkey	Cassady K. Human cytomegalovirus TRS1 and IRS1 gene products block the double-stranded-RNA-activated host protein shutoff response induced by herpes simplex virus type 1 infection. J Virol. 2005;79:8707-15 pubmed
	Chen M, Hsu L, Wang S, Pan Y, Lo J, Yeh T, et al. Salubrinal Enhances Cancer Cell Death during Glucose Deprivation through the Upregulation of xCT and Mitochondrial Oxidative Stress. Biomedicines. 2021;9: pubmed publisher
	Gabaev I, Williamson J, Crozier T, Schulz T, Lehner P. Quantitative Proteomics Analysis of Lytic KSHV Infection in Human Endothelial Cells Reveals Targets of Viral Immune Modulation. Cell Rep. 2020;33:108249 pubmed publisher
	Ortega J, Daley E, Kour S, Samani M, Tellez L, Smith H, et al. Nucleocytoplasmic Proteomic Analysis Uncovers eRF1 and Nonsense-Mediated Decay as Modifiers of ALS/FTD C9orf72 Toxicity. Neuron. 2020;106:90-107.e13 pubmed publisher
	Rubio R, Mohr I. Inhibition of ULK1 and Beclin1 by an α-herpesvirus Akt-like Ser/Thr kinase limits autophagy to stimulate virus replication. Proc Natl Acad Sci U S A. 2019;: pubmed publisher
	Claes Z, Jonkhout M, Crespillo Casado A, Bollen M. The antibiotic robenidine exhibits guanabenz-like cytoprotective properties by a mechanism independent of protein phosphatase PP1:PPP1R15A. J Biol Chem. 2019;294:13478-13486 pubmed publisher
	Wang Y, Chien Y, Hsiao W, Wang C, Wang S. Fission Yeast Asc1 Stabilizes the Interaction between Eukaryotic Initiation Factor 3a and Rps0A/uS2 for Protein Synthesis. Mol Cell Biol. 2019;39: pubmed publisher
	Patel V, Bidault G, Chambers J, Carobbio S, Everden A, Garcés C, et al. Inactivation of Ppp1r15a minimises weight gain and insulin resistance during caloric excess in female mice. Sci Rep. 2019;9:2903 pubmed publisher
	Pan D, Li G, Morris Love J, Qi S, Feng L, Mertens M, et al. Herpes Simplex Virus 1 Lytic Infection Blocks MicroRNA (miRNA) Biogenesis at the Stage of Nuclear Export of Pre-miRNAs. MBio. 2019;10: pubmed publisher
	Silva R, Castilho B, Sattlegger E. A Rapid Extraction Method for mammalian cell cultures, suitable for quantitative immunoblotting analysis of proteins, including phosphorylated GCN2 and eIF2α. MethodsX. 2018;5:75-82 pubmed publisher
	Bittremieux M, La Rovere R, Schuermans M, Luyten T, Mikoshiba K, Vangheluwe P, et al. Extracellular and ER-stored Ca2+ contribute to BIRD-2-induced cell death in diffuse large B-cell lymphoma cells. Cell Death Discov. 2018;4:101 pubmed publisher
	Jeon Y, Shin J, Lee S, Lee Y, Kim J, Kwon H, et al. Angelica gigas Nakai Has Synergetic Effects on Doxorubicin-Induced Apoptosis. Biomed Res Int. 2018;2018:6716547 pubmed publisher
	Teo C, O Hare P. A bimodal switch in global protein translation coupled to eIF4H relocalisation during advancing cell-cell transmission of herpes simplex virus. PLoS Pathog. 2018;14:e1007196 pubmed publisher
	Burgess H, Pourchet A, Hajdu C, Chiriboga L, Frey A, Mohr I. Targeting Poxvirus Decapping Enzymes and mRNA Decay to Generate an Effective Oncolytic Virus. Mol Ther Oncolytics. 2018;8:71-81 pubmed publisher
	Taga M, Mouton Liger F, Sadoune M, Gourmaud S, Norman J, Tible M, et al. PKR modulates abnormal brain signaling in experimental obesity. PLoS ONE. 2018;13:e0196983 pubmed publisher
	Liu X, Main D, Ma Y, He B. Herpes Simplex Virus 1 Inhibits TANK-Binding Kinase 1 through Formation of the Us11-Hsp90 Complex. J Virol. 2018;92: pubmed publisher
	Frey T, Forsyth K, Sheehan M, De Haven B, Pevarnik J, Hand E, et al. Ectromelia virus lacking the E3L ortholog is replication-defective and nonpathogenic but does induce protective immunity in a mouse strain susceptible to lethal mousepox. Virology. 2018;518:335-348 pubmed publisher
	Nakashima H, Nguyen T, Kasai K, Passaro C, Ito H, Goins W, et al. Toxicity and Efficacy of a Novel GADD34-expressing Oncolytic HSV-1 for the Treatment of Experimental Glioblastoma. Clin Cancer Res. 2018;24:2574-2584 pubmed publisher
	Hurwitz S, Cheerathodi M, Nkosi D, York S, Meckes D. Tetraspanin CD63 Bridges Autophagic and Endosomal Processes To Regulate Exosomal Secretion and Intracellular Signaling of Epstein-Barr Virus LMP1. J Virol. 2018;92: pubmed publisher
	Manivanh R, Mehrbach J, Knipe D, Leib D. Role of Herpes Simplex Virus 1 γ34.5 in the Regulation of IRF3 Signaling. J Virol. 2017;91: pubmed publisher
	Demirtas L, Guclu A, Erdur F, Akbas E, Ozçiçek A, Onk D, et al. Apoptosis, autophagy & endoplasmic reticulum stress in diabetes mellitus. Indian J Med Res. 2016;144:515-524 pubmed publisher
	To M, Peterson C, Roberts M, Counihan J, Wu T, Forster M, et al. Lipid disequilibrium disrupts ER proteostasis by impairing ERAD substrate glycan trimming and dislocation. Mol Biol Cell. 2017;28:270-284 pubmed publisher
	Hodgson L, Spiering D, Sabouri Ghomi M, Dagliyan O, DerMardirossian C, Danuser G, et al. FRET binding antenna reports spatiotemporal dynamics of GDI-Cdc42 GTPase interactions. Nat Chem Biol. 2016;12:802-809 pubmed publisher
	Nelson E, Schmidt K, DeflubÃ© L, DoÄŸanay S, Banadyga L, Olejnik J, et al. Ebola Virus Does Not Induce Stress Granule Formation during Infection and Sequesters Stress Granule Proteins within Viral Inclusions. J Virol. 2016;90:7268-7284 pubmed publisher
	Blas Rus N, Bustos Morán E, Perez de Castro I, de Carcer G, Borroto A, Camafeita E, et al. Aurora A drives early signalling and vesicle dynamics during T-cell activation. Nat Commun. 2016;7:11389 pubmed publisher
	Wanders D, Stone K, Forney L, Cortez C, Dille K, Simon J, et al. Role of GCN2-Independent Signaling Through a Noncanonical PERK/NRF2 Pathway in the Physiological Responses to Dietary Methionine Restriction. Diabetes. 2016;65:1499-510 pubmed publisher
	Messner B, TÃ¼rkcan A, Ploner C, Laufer G, Bernhard D. Cadmium overkill: autophagy, apoptosis and necrosis signalling in endothelial cells exposed to cadmium. Cell Mol Life Sci. 2016;73:1699-713 pubmed publisher
	Niehues S, Bussmann J, Steffes G, Erdmann I, KÃ¶hrer C, Sun L, et al. Impaired protein translation in Drosophila models for Charcot-Marie-Tooth neuropathy caused by mutant tRNA synthetases. Nat Commun. 2015;6:7520 pubmed publisher
	Chambers J, Dalton L, Clarke H, Malzer E, Dominicus C, Patel V, et al. Actin dynamics tune the integrated stress response by regulating eukaryotic initiation factor 2α dephosphorylation. elife. 2015;4: pubmed publisher
	Thelen M, Damme M, DaÎ¼Î¼e M, Schweizer M, Hagel C, Wong A, et al. Disruption of the autophagy-lysosome pathway is involved in neuropathology of the nclf mouse model of neuronal ceroid lipofuscinosis. PLoS ONE. 2012;7:e35493 pubmed publisher
	Trottier M, Palian B, Reiss C. VSV replication in neurons is inhibited by type I IFN at multiple stages of infection. Virology. 2005;333:215-25 pubmed
	Ramos Castaneda J, Park Y, Liu M, Hauser K, Rudolph H, Shull G, et al. Deficiency of ATP2C1, a Golgi ion pump, induces secretory pathway defects in endoplasmic reticulum (ER)-associated degradation and sensitivity to ER stress. J Biol Chem. 2005;280:9467-73 pubmed
	Zhang P, McGrath B, Li S, Frank A, Zambito F, Reinert J, et al. The PERK eukaryotic initiation factor 2 alpha kinase is required for the development of the skeletal system, postnatal growth, and the function and viability of the pancreas. Mol Cell Biol. 2002;22:3864-74 pubmed

product information

Product Type :

Antibody

Product Name :

EIF2S1 Monoclonal Antibody (EIF2-alpha)

Catalog # :

AHO0802

Quantity :

100 µg

Price :

US 450.00

Clonality :

Monoclonal

Purity :

Protein A/G

Host :

Mouse

Reactivity :

Human, Mouse, Non-human primate, Rat

Applications :

Flow Cytometry: 3-5 µg/1x10^6 cells, Immunocytochemistry: 1 µg/mL, Immunohistochemistry (Paraffin): 1:10-1:50, Immunoprecipitation: Assay-dependent, RNA Immunoprecipitation: Assay-dependent, Western Blot: 1:500-1:1,000

Species :

Human, Mouse, Non-human primate, Rat

Clone :

EIF2-alpha

Isotype :

IgG1

Storage :

-20°C

Description :

EIF2a (Eukaryotic translation initiation factor 2A) is a heterotrimer composed of three subunits (alpha, beta, and gamma). This translation initiation factor drives binding of initiator methionyl-tRNA to the 40S ribosome in an AUG-dependent manner to form the 43S pre-initiation complex. The polypeptide can be phosphorylated by related protein kinases activated in response to stress. Phosphorylated EIF2A inhibits EIF2B activity and prevents guanine nucleotide exchange.

Immunogen :

Recombinant human eIF-2-alpha.

Format :

Liquid

Applications w/Dilutions :

Aliases :

0910001O23Rik; 2410026C18Rik; 35kDa; EIF2; EIF-2; eIF2 alpha; eIF2 alpha subunit; EIF2A; eIF-2A; eIF2alpha; EIF-2alpha; eIF-2-alpha; EIF2S1; eif2s1b; Eukaryotic translation initiation factor 2 subunit 1; eukaryotic translation initiation factor 2 subunit 1b; eukaryotic translation initiation factor 2 subunit alpha; eukaryotic translation initiation factor 2, subunit 1 alpha; eukaryotic translation initiation factor 2, subunit 1 alpha b; eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa; eukaryotic translation initiation factor 2A; fb19d01; translation initiation factor subunit; wu:fb19d01

more info or order :

Invitrogen product webpage