product summary
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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
Paxillin Monoclonal Antibody (5H11)
catalog :
AHO0492
quantity :
500 µL
price :
US 436.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
5H11
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
more info or order :
Invitrogen product webpage
citations: 21
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; human; loading ...; fig 6b
Park S, Lee C, Choi J, Kim J, Lee W, Jang T, et al. Dysadherin awakens mechanical forces and promotes colorectal cancer progression. Theranostics. 2022;12:4399-4414 pubmed publisher
Mohanty A, Nam A, Srivastava S, Jones J, Lomenick B, Singhal S, et al. Acquired resistance to KRAS G12C small-molecule inhibitors via genetic/nongenetic mechanisms in lung cancer. Sci Adv. 2023;9:eade3816 pubmed publisher
Nairon K, DePalma T, Zent J, Leight J, Skardal A. Tumor cell-conditioned media drives collagen remodeling via fibroblast and pericyte activation in an in vitro premetastatic niche model. iScience. 2022;25:104645 pubmed publisher
Hamasaki E, Wakita N, Yasuoka H, Nagaoka H, Morita M, Takashima E, et al. The Lipid-Binding Defective Dynamin 2 Mutant in Charcot-Marie-Tooth Disease Impairs Proper Actin Bundling and Actin Organization in Glomerular Podocytes. Front Cell Dev Biol. 2022;10:884509 pubmed publisher
Junqueira Alves C, Dariolli R, Haydak J, Kang S, Hannah T, Wiener R, et al. Plexin-B2 orchestrates collective stem cell dynamics via actomyosin contractility, cytoskeletal tension and adhesion. Nat Commun. 2021;12:6019 pubmed publisher
Mousson A, Legrand M, Steffan T, Vauchelles R, Carl P, Gies J, et al. Inhibiting FAK-Paxillin Interaction Reduces Migration and Invadopodia-Mediated Matrix Degradation in Metastatic Melanoma Cells. Cancers (Basel). 2021;13: pubmed publisher
Rabanal Ruiz Y, Byron A, Wirth A, Madsen R, Sedlackova L, Hewitt G, et al. mTORC1 activity is supported by spatial association with focal adhesions. J Cell Biol. 2021;220: pubmed publisher
Iendaltseva O, Orlova V, Mummery C, Danen E, Schmidt T. Fibronectin Patches as Anchoring Points for Force Sensing and Transmission in Human Induced Pluripotent Stem Cell-Derived Pericytes. Stem Cell Reports. 2020;14:1107-1122 pubmed publisher
Bersini S, Lytle N, Schulte R, Huang L, Wahl G, Hetzer M. Nup93 regulates breast tumor growth by modulating cell proliferation and actin cytoskeleton remodeling. Life Sci Alliance. 2020;3: pubmed publisher
Ichikawa T, Kita M, Matsui T, Nagasato A, Araki T, Chiang S, et al. Vinexin family (SORBS) proteins play different roles in stiffness-sensing and contractile force generation. J Cell Sci. 2017;130:3517-3531 pubmed publisher
Busby T, Meissner J, Styers M, Bhatt J, Kaushik A, Hjelmeland A, et al. The Arf activator GBF1 localizes to plasma membrane sites involved in cell adhesion and motility. Cell Logist. 2017;7:e1308900 pubmed publisher
Juanes M, Bouguenina H, Eskin J, Jaiswal R, Badache A, Goode B. Adenomatous polyposis coli nucleates actin assembly to drive cell migration and microtubule-induced focal adhesion turnover. J Cell Biol. 2017;216:2859-2875 pubmed publisher
Yang X, Mei S, Niu H, Li J. Nicotinic acid impairs assembly of leading edge in glioma cells. Oncol Rep. 2017;38:829-836 pubmed publisher
Chung I, Reichelt M, Shao L, Akita R, Koeppen H, Rangell L, et al. High cell-surface density of HER2 deforms cell membranes. Nat Commun. 2016;7:12742 pubmed publisher
Teräväinen T, Myllymäki S, Friedrichs J, Strohmeyer N, Moyano J, Wu C, et al. ?V-integrins are required for mechanotransduction in MDCK epithelial cells. PLoS ONE. 2013;8:e71485 pubmed publisher
Kawada I, Hasina R, Lennon F, Bindokas V, Usatyuk P, Tan Y, et al. Paxillin mutations affect focal adhesions and lead to altered mitochondrial dynamics: relevance to lung cancer. Cancer Biol Ther. 2013;14:679-91 pubmed publisher
Xiao X, Mruk D, Lee W, Cheng C. c-Yes regulates cell adhesion at the blood-testis barrier and the apical ectoplasmic specialization in the seminiferous epithelium of rat testes. Int J Biochem Cell Biol. 2011;43:651-65 pubmed publisher
Waschbüsch D, Born S, Niediek V, Kirchgessner N, Tamboli I, Walter J, et al. Presenilin 1 affects focal adhesion site formation and cell force generation via c-Src transcriptional and posttranslational regulation. J Biol Chem. 2009;284:10138-49 pubmed publisher
Beardsley A, Fang K, Mertz H, Castranova V, Friend S, Liu J. Loss of caveolin-1 polarity impedes endothelial cell polarization and directional movement. J Biol Chem. 2005;280:3541-7 pubmed
Deo D, Bazan N, Hunt J. Activation of platelet-activating factor receptor-coupled G alpha q leads to stimulation of Src and focal adhesion kinase via two separate pathways in human umbilical vein endothelial cells. J Biol Chem. 2004;279:3497-508 pubmed
Grace E, Busciglio J. Aberrant activation of focal adhesion proteins mediates fibrillar amyloid beta-induced neuronal dystrophy. J Neurosci. 2003;23:493-502 pubmed
product information
Product Type :
Antibody
Product Name :
Paxillin Monoclonal Antibody (5H11)
Catalog # :
AHO0492
Quantity :
500 µL
Price :
US 436.00
Clonality :
Monoclonal
Purity :
Protein G
Host :
Mouse
Reactivity :
Human, Mouse, Rat
Applications :
Immunocytochemistry: 2 µg/mL, Immunohistochemistry (Paraffin): 1-2 µg/mL, Immunoprecipitation: 2 µg/mL, Western Blot: Assay-Dependent
Species :
Human, Mouse, Rat
Clone :
5H11
Isotype :
IgG1, kappa
Storage :
4° C
Description :
Paxillin is a focal adhesion protein and a substrate for several tyrosine kinases such as src, FAK, and p210BRC/ABL. The tyrosine phosphorylation of paxillin is affected by conditions that change cell-cell adhesion. Paxillin associates tightly with FAK and Crk through its SH2 domain. This interaction is independent of the extracellular matrix. Although paxillin was initially discovered in fibroblasts, its phosphorylation may also be important during neurite extension during differentiation.
Immunogen :
Purified recombinant full-length human paxillin protein.
Format :
Liquid
Applications w/Dilutions :
Immunocytochemistry: 2 µg/mL, Immunohistochemistry (Paraffin): 1-2 µg/mL, Immunoprecipitation: 2 µg/mL, Western Blot: Assay-Dependent
Aliases :
adaptor protein; AW108311; AW123232; FLJ16691; hCG_1778014; I79_001229; myocardial ischemic preconditioning associated protein 7; Pax; PAX-1; PAXI; Paxillin; paxillin a; paxillin alpha; pxn; pxna; testicular tissue protein Li 134; TGFB1I1; transforming growth factor beta 1 induced transcript 1; wu:fw71f12
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA