p53 Monoclonal Antibody (PAb 240) | Invitrogen AHO0112 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

p53 Monoclonal Antibody (PAb 240)

catalog :

AHO0112

quantity :

500 µL

price :

US 506.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

PAb 240

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

citations: 9

Reference
Pollet M, Shaik S, Mescher M, Frauenstein K, Tigges J, Braun S, et al. The AHR represses nucleotide excision repair and apoptosis and contributes to UV-induced skin carcinogenesis. Cell Death Differ. 2018;25:1823-1836 pubmed publisher
Ventura A, Do Nascimento A, dos Santos M, Vieira Lopes D, Sales A, Pinheiro N. Analysis of the expression of p53 during the morphogenesis of the gastroesophageal mucosa of Gallus gallus domesticus (Linnaeus, 1758). Cell Tissue Res. 2014;355:111-9 pubmed publisher
Glukhova V, Tomazela D, Findlay G, Monnat R, MacCoss M. Rapid assessment of RNAi-mediated protein depletion by selected reaction monitoring mass spectrometry. J Proteome Res. 2013;12:3246-54 pubmed publisher
Rebel H, Bodmann C, van de Glind G, de Gruijl F. UV-induced ablation of the epidermal basal layer including p53-mutant clones resets UV carcinogenesis showing squamous cell carcinomas to originate from interfollicular epidermis. Carcinogenesis. 2012;33:714-20 pubmed publisher
Agrawal A, Sharma M, Rai S, Singh B, Tiwari M, Chandra R. The effect of the aqueous extract of the roots of Asparagus racemosus on hepatocarcinogenesis initiated by diethylnitrosamine. Phytother Res. 2008;22:1175-82 pubmed publisher
Preyer M, Shu C, Wang J. Delayed activation of Bax by DNA damage in embryonic stem cells with knock-in mutations of the Abl nuclear localization signals. Cell Death Differ. 2007;14:1139-48 pubmed
Rosas Acosta G, Russell W, Deyrieux A, Russell D, Wilson V. A universal strategy for proteomic studies of SUMO and other ubiquitin-like modifiers. Mol Cell Proteomics. 2005;4:56-72 pubmed
Mathur S, Kaur P, Sharma M, Katyal A, Singh B, Tiwari M, et al. The treatment of skin carcinoma, induced by UV B radiation, using 1-oxo-5beta, 6beta-epoxy-witha-2-enolide, isolated from the roots of Withania somnifera, in a rat model. Phytomedicine. 2004;11:452-60 pubmed
Albaric O, Bret L, Amardeihl M, Delverdier M. Immunohistochemical expression of p53 in animal tumors: a methodological study using four anti-human p53 antibodies. Histol Histopathol. 2001;16:113-21 pubmed publisher

product information

Product Type :

Antibody

Product Name :

p53 Monoclonal Antibody (PAb 240)

Catalog # :

AHO0112

Quantity :

500 µL

Price :

US 506.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Human, Mouse

Applications :

Flow Cytometry: Assay-dependent, Immunohistochemistry (Paraffin): Assay-Dependent, Immunoprecipitation: Assay-Dependent, Western Blot: 1-2 µg/mL

Species :

Human, Mouse

Clone :

PAb 240

Isotype :

IgG1

Storage :

4° C

Description :

The tumor suppressor protein, p53, is a sequence specific transcription factor that is activated by cellular stress. p53 mediates cell cycle arrest or apoptosis in response to DNA damage or starvation for pyrimidine nucleotides. p53 is up-regulated in response to stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest. The structure of p53 comprises an N-terminal transactivation domain, a central DNA-binding domain, an oligomerisation domain, and a C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization. p53 has been characterized to play a role in blocking the proliferative action of damaged cells and act as an anticancer agent. Phosphorylation of Ser392 in p53 has been shown to associate with the formation of human tumors. In addition, p53 has also been linked to the effects of aging and oxidative stress and an increase in p53 has been linked to deficits in LTP (Long Term Potentiation) in learning and memory. p53 is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and cause the loss of tumor suppressor activity. Alterations of the TP53 gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families such as Li-Fraumeni syndrome.

Immunogen :

Murine p53-beta-galactosidase fusion protein containing p53 sequence from amino acid14-289.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: Assay-dependent, Immunohistochemistry (Paraffin): Assay-Dependent, Immunoprecipitation: Assay-Dependent, Western Blot: 1-2 µg/mL

Aliases :

Antigen NY-CO-13; bbl; BCC7; bfy; bhy; cellular tumor antigen p53; Cys 51 Stop; EGK_08142; FLJ92943; HGNC11998; I79_002739; LFS1; Li-Fraumeni syndrome; mutant p53; mutant tumor protein 53; OTTMUSP00000006194; p44; p53; p53 cellular tumor antigen; p53 protein; p53 tumor suppressor; p53 tumor suppressor phosphoprotein; phosphoprotein p53; Tp53; tp53.L; transformation related protein 53; transformation-related protein 53; Trp248; Trp53; tumor protein 53; tumor protein p53; tumor protein p53 (Li-Fraumeni syndrome); tumor protein p53 L homeolog; tumor suppressor p53; tumor suppressor p53 phosphoprotein; tumor suppressor protein p53; tumor supressor p53; Tumour Protein p53; XELAEV_180196761mg; Xp53; Xrel3