Cyclin D1 Monoclonal Antibody (DCS-6) | Invitrogen AHF0082 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Cyclin D1 Monoclonal Antibody (DCS-6)

catalog :

AHF0082

quantity :

100 µg

price :

US 496.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

DCS-6

reactivity :

human, mouse, rat, dogs

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Invitrogen product webpage

citations: 17

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig 4	Tian L, Wang C, Hagen F, Gormley M, Addya S, Soccio R, et al. Acetylation-defective mutant of PparÎ³ is associated with decreased lipid synthesis in breast cancer cells. Oncotarget. 2014;5:7303-15 pubmed
western blot; human; 1:1000; fig 1e	Ormanns S, Neumann J, Horst D, Kirchner T, Jung A. WNT signaling and distant metastasis in colon cancer through transcriptional activity of nuclear ?-Catenin depend on active PI3K signaling. Oncotarget. 2014;5:2999-3011 pubmed
western blot; human; fig s1e	Comstock C, Augello M, Goodwin J, de Leeuw R, Schiewer M, Ostrander W, et al. Targeting cell cycle and hormone receptor pathways in cancer. Oncogene. 2013;32:5481-91 pubmed publisher
western blot; mouse	Mitterberger M, Zwerschke W. Mechanisms of resveratrol-induced inhibition of clonal expansion and terminal adipogenic differentiation in 3T3-L1 preadipocytes. J Gerontol A Biol Sci Med Sci. 2013;68:1356-76 pubmed publisher
western blot; mouse; fig 1	Chen S, Fu S, Hsu S, Huang Y, Hsu B. Synergistic Effect of Hyperglycemia and p27(kip1) Suppression on Adult Mouse Islet Beta Cell Replication. Int J Endocrinol. 2012;2012:417390 pubmed publisher
western blot; human; fig 1	Akli S, Zhang X, Bondaruk J, Tucker S, Czerniak P, Benedict W, et al. Low molecular weight cyclin E is associated with p27-resistant, high-grade, high-stage and invasive bladder cancer. Cell Cycle. 2012;11:1468-76 pubmed publisher
immunocytochemistry; human; 1:100; fig 4	Korgun E, Unek G, Herrera E, Jones C, Wadsack C, Kipmen Korgun D, et al. Mapping of CIP/KIP inhibitors, G1 cyclins D1, D3, E and p53 proteins in the rat term placenta. Histochem Cell Biol. 2011;136:267-78 pubmed publisher
western blot; human; 1:250; fig 2	Chimploy K, Díaz G, Li Q, Carter O, Dashwood W, Mathews C, et al. E2F4 and ribonucleotide reductase mediate S-phase arrest in colon cancer cells treated with chlorophyllin. Int J Cancer. 2009;125:2086-94 pubmed publisher
western blot; human	Tiedemann R, Schmidt J, Keats J, Shi C, Zhu Y, Palmer S, et al. Identification of a potent natural triterpenoid inhibitor of proteosome chymotrypsin-like activity and NF-kappaB with antimyeloma activity in vitro and in vivo. Blood. 2009;113:4027-37 pubmed publisher
western blot; human	Kajanne R, Leppa S, Luukkainen P, Ustinov J, Thiel A, Ristimaki A, et al. Hydrocortisone and indomethacin negatively modulate EGF-R signaling in human fetal intestine. Pediatr Res. 2007;62:570-5 pubmed
western blot; rat; 1:150; fig 3	Rao H, Thirumangalakudi L, Desmond P, Grammas P. Cyclin D1, cdk4, and Bim are involved in thrombin-induced apoptosis in cultured cortical neurons. J Neurochem. 2007;101:498-505 pubmed
western blot; rat; 1:200; loading ...; fig 1b	Friedrichsen B, Richter H, Hansen J, Rhodes C, Nielsen J, Billestrup N, et al. Signal transducer and activator of transcription 5 activation is sufficient to drive transcriptional induction of cyclin D2 gene and proliferation of rat pancreatic beta-cells. Mol Endocrinol. 2003;17:945-58 pubmed
western blot; human; fig 6	Law B, Chytil A, Dumont N, Hamilton E, Waltner Law M, Aakre M, et al. Rapamycin potentiates transforming growth factor beta-induced growth arrest in nontransformed, oncogene-transformed, and human cancer cells. Mol Cell Biol. 2002;22:8184-98 pubmed
	Singh A, Devkar R, Basu A. Myeloid Differentiation Primary Response 88-Cyclin D1 Signaling in Breast Cancer Cells Regulates Toll-Like Receptor 3-Mediated Cell Proliferation. Front Oncol. 2020;10:1780 pubmed publisher
	Ji Q, Qi D, Xu X, Xu Y, Goodman S, Kang L, et al. Cryptotanshinone Protects Cartilage against Developing Osteoarthritis through the miR-106a-5p/GLIS3 Axis. Mol Ther Nucleic Acids. 2018;11:170-179 pubmed publisher
	Wang H, Muguira M, Liu W, Zhang T, Chen C, Aucoin R, et al. Identification of an INSM1-binding site in the insulin promoter: negative regulation of the insulin gene transcription. J Endocrinol. 2008;198:29-39 pubmed publisher
	Mermelshtein A, Gerson A, Walfisch S, Delgado B, Shechter Maor G, Delgado J, et al. Expression of D-type cyclins in colon cancer and in cell lines from colon carcinomas. Br J Cancer. 2005;93:338-45 pubmed

product information

Product Type :

Antibody

Product Name :

Cyclin D1 Monoclonal Antibody (DCS-6)

Catalog # :

AHF0082

Quantity :

100 µg

Price :

US 496.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Canine, Human, Mouse, Non-human primate, Rat

Applications :

Immunocytochemistry: 1-2 µg/mL, Immunohistochemistry: Assay-dependent, Immunoprecipitation: Assay-Dependent, Inhibition Assays: Assay-dependent, Western Blot: 1:500-1:1,000

Species :

Canine, Human, Mouse, Non-human primate, Rat

Clone :

DCS-6

Isotype :

IgG2a, kappa

Storage :

4° C

Description :

Cyclin D1 (PRAD-1, bcl-1) is one of the key cell cycle regulators, and functions in association with cdk4 and/or cdk6 by phosphorylating the Rb protein. Cyclin D1 is a putative proto-oncogene overexpressed in a wide variety of human neoplasms including mantle cell lymphomas (MCL). In addition, cyclin D1 positively regulates protein phosphorylation, mammary gland epithelial cell proliferation, and fat cell differentiation. In humans, the CCND1 gene encoding cyclin D1 is present on chromosome 11. Cyclin D1 is a cytoplasmic and nuclear protein that is synthesized during G1 phase and assembles with either cyclin-dependent kinase 4 (CDK4) or CDK6 in response to growth factor stimulation. D-type cyclin-CDK complexes act to inactivate the growth-suppressive function of the Rb protein through its phosphorylation, and titrate CDK inhibitors such as p21Cip1 and p27Kip1. Without growth factor-mediated stimulation, Cyclin D1 is unstable, and undergoes ubiquitin-mediated degradation, which is triggered by its phosphorylation. Cyclin D1 destabilization participates in G1/S phase arrest. The Cyclin D1 protein belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclin D1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. The Cyclin D1 protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. Cyclin D1 has been successfully employed and is a promising tool for further studies in both cell cycle biology and cancer associated abnormalities.

Immunogen :

Human recombinant full length cyclin D1 protein.

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1-2 µg/mL, Immunohistochemistry: Assay-dependent, Immunoprecipitation: Assay-Dependent, Inhibition Assays: Assay-dependent, Western Blot: 1:500-1:1,000

Aliases :

AI327039; B-cell CLL/lymphoma 1; B-cell lymphoma 1 protein; BCL1; BCL-1; BCL-1 oncogene; CCND1; CCND1 protein; cD1; cDNA; CycD1; cyclin D1; Cyl-1; D11S287E; FLJ93625; G1/S-specific cyclin-D1; mRNA; PRAD1; PRAD-1; PRAD1 oncogene; U21B31

more info or order :

Invitrogen product webpage