This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
TNF alpha Antibody Cocktail
catalog :
AHC3712
quantity :
500 µg
price :
US 446
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
68B2B3 and 68B6A3
reactivity :
human, pigs
application :
immunohistochemistry
citations: 18
Published Application/Species/Sample/DilutionReference
  • ELISA; human; 1:1000; fig 6d
Rodriguez E, Boelaars K, Brown K, Madunić K, van Ee T, Dijk F, et al. Analysis of the glyco-code in pancreatic ductal adenocarcinoma identifies glycan-mediated immune regulatory circuits. Commun Biol. 2022;5:41 pubmed publisher
  • immunohistochemistry; pigs ; 1:100; fig 4
Kvist P, Iburg T, Bielecki M, Gerstenberg M, Buch Rasmussen T, Hasselager E, et al. Biocompatibility of electrochemical glucose sensors implanted in the subcutis of pigs. Diabetes Technol Ther. 2006;8:463-75 pubmed
  • ELISA; human; tbl 2
Inglefield J, Larson C, Gibson S, Lebrec H, Miller R. Apoptotic responses in squamous carcinoma and epithelial cells to small-molecule toll-like receptor agonists evaluated with automated cytometry. J Biomol Screen. 2006;11:575-85 pubmed
  • ELISA; human; 8 ug/ml
Njemini R, Lambert M, Demanet C, Mets T. The effect of aging and inflammation on heat shock protein 27 in human monocytes and lymphocytes. Exp Gerontol. 2006;41:312-9 pubmed
  • ELISA; human; fig 4
Shen C, Maerten P, Geboes K, Van Assche G, Rutgeerts P, Ceuppens J. Infliximab induces apoptosis of monocytes and T lymphocytes in a human-mouse chimeric model. Clin Immunol. 2005;115:250-9 pubmed
  • ELISA; human; fig 1
Arredouani M, Kasran A, Vanoirbeek J, Berger F, Baumann H, Ceuppens J. Haptoglobin dampens endotoxin-induced inflammatory effects both in vitro and in vivo. Immunology. 2005;114:263-71 pubmed
  • ELISA; human
Vinogradov E, Paul C, Li J, Zhou Y, Lyle E, Tapping R, et al. The structure and biological characteristics of the Spirochaeta aurantia outer membrane glycolipid LGLB. Eur J Biochem. 2004;271:4685-95 pubmed
  • ELISA; human; 5 ug/ml; fig 1
Gee K, Kozlowski M, Kumar A. Tumor necrosis factor-alpha induces functionally active hyaluronan-adhesive CD44 by activating sialidase through p38 mitogen-activated protein kinase in lipopolysaccharide-stimulated human monocytic cells. J Biol Chem. 2003;278:37275-87 pubmed
  • ELISA; human; 5 ug/ml; fig 2
Gee K, Lim W, Ma W, Nandan D, Diaz Mitoma F, Kozlowski M, et al. Differential regulation of CD44 expression by lipopolysaccharide (LPS) and TNF-alpha in human monocytic cells: distinct involvement of c-Jun N-terminal kinase in LPS-induced CD44 expression. J Immunol. 2002;169:5660-72 pubmed
  • ELISA; human; 1 ug/ml
Soop M, Duxbury H, Agwunobi A, Gibson J, Hopkins S, Childs C, et al. Euglycemic hyperinsulinemia augments the cytokine and endocrine responses to endotoxin in humans. Am J Physiol Endocrinol Metab. 2002;282:E1276-85 pubmed
  • ELISA; human
Kooijman R, Coppens A, Hooghe Peters E. Igf-I inhibits spontaneous apoptosis in human granulocytes. Endocrinology. 2002;143:1206-12 pubmed
  • ELISA; human
Braun M, Wang J, Lahey E, Rabin R, Kelsall B. Activation of the formyl peptide receptor by the HIV-derived peptide T-20 suppresses interleukin-12 p70 production by human monocytes. Blood. 2001;97:3531-6 pubmed
  • ELISA; human; fig 2, 3
Bullens D, Rafiq K, Charitidou L, Peng X, Kasran A, Warmerdam P, et al. Effects of co-stimulation by CD58 on human T cell cytokine production: a selective cytokine pattern with induction of high IL-10 production. Int Immunol. 2001;13:181-91 pubmed
  • ELISA; human; fig 1
Tapping R, Akashi S, Miyake K, Godowski P, Tobias P. Toll-like receptor 4, but not toll-like receptor 2, is a signaling receptor for Escherichia and Salmonella lipopolysaccharides. J Immunol. 2000;165:5780-7 pubmed
  • ELISA; human
Braun M, Lahey E, Kelsall B. Selective suppression of IL-12 production by chemoattractants. J Immunol. 2000;164:3009-17 pubmed
  • ELISA; human
Braun M, He J, Wu C, Kelsall B. Cholera toxin suppresses interleukin (IL)-12 production and IL-12 receptor beta1 and beta2 chain expression. J Exp Med. 1999;189:541-52 pubmed
Byla P, Andersen M, Holtet T, Jacobsen H, Munch M, Gad H, et al. Selection of a novel and highly specific tumor necrosis factor alpha (TNFalpha) antagonist: insight from the crystal structure of the antagonist-TNFalpha complex. J Biol Chem. 2010;285:12096-100 pubmed publisher
Gorden K, Gorski K, Gibson S, Kedl R, Kieper W, Qiu X, et al. Synthetic TLR agonists reveal functional differences between human TLR7 and TLR8. J Immunol. 2005;174:1259-68 pubmed
product information
Product Type :
Antibody
Product Name :
TNF alpha Antibody Cocktail
Catalog # :
AHC3712
Quantity :
500 µg
Price :
US 446
Clonality :
Cocktail
Purity :
protein A
Host :
Mouse
Reactivity :
Human
Applications :
ELISA: Assay-dependent
Species :
Human
Clone :
68B2B3 and 68B6A3
Isotype :
Ig Mix
Storage :
4° C
Description :
TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.
Immunogen :
Purified recombinant human TNF-alpha.
Format :
Liquid
Applications w/Dilutions :
ELISA: Assay-dependent
Aliases :
APC1 protein; Cachectin; C-domain 1; C-domain 2; cTNF; DADB-70P7.1; DIF; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; RATTNF; Tnf; TNF alpha; TNF superfamily; TNF a; TNF, macrophage-derived; TNF, monocyte-derived; TNFA; TNF-a; TNFalpha; TNF-alpha; Tnfsf1a; TNFSF2; TNFa; TNLG1F; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor alpha (cachetin); tumor necrosis factor alpha precursor; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha; tumor necrosis factor-alpha precursor; tumor-necrosis factor; tumour necrosis factor
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA