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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
IL-10 Monoclonal Antibody (JES3-9D7)
catalog :
AHC0103
quantity :
1 mg
price :
US 468.00
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
JES3-9D7
reactivity :
African green monkey, human, rhesus macaque
application :
ELISA, immunohistochemistry, neutralization, flow cytometry, immunohistochemistry - paraffin section, blocking or activating experiments
more info or order :
Invitrogen product webpage
citations: 35
Published Application/Species/Sample/DilutionReference
  • ELISA; human; loading ...; fig 5c
Otsuka Y, Watanabe E, Shinya E, Okura S, Saeki H, Geijtenbeek T, et al. Differentiation of Langerhans Cells from Monocytes and Their Specific Function in Inducing IL-22-Specific Th Cells. J Immunol. 2018;201:3006-3016 pubmed publisher
  • flow cytometry; human; loading ...; fig 3b
Pizzolla A, Nguyen T, Sant S, Jaffar J, Loudovaris T, Mannering S, et al. Influenza-specific lung-resident memory T cells are proliferative and polyfunctional and maintain diverse TCR profiles. J Clin Invest. 2018;128:721-733 pubmed publisher
  • flow cytometry; human; loading ...; tbl 1
Sumatoh H, Teng K, Cheng Y, Newell E. Optimization of mass cytometry sample cryopreservation after staining. Cytometry A. 2017;91:48-61 pubmed publisher
  • flow cytometry; rhesus macaque; fig 6f
  • flow cytometry; human; fig 1e
Miles B, Miller S, Folkvord J, Levy D, Rakasz E, Skinner P, et al. Follicular Regulatory CD8 T Cells Impair the Germinal Center Response in SIV and Ex Vivo HIV Infection. PLoS Pathog. 2016;12:e1005924 pubmed publisher
  • flow cytometry; human; loading ...; fig 6c
Neumann L, Mueller M, Moos V, Heller F, Meyer T, Loddenkemper C, et al. Mucosal Inducible NO Synthase-Producing IgA+ Plasma Cells in Helicobacter pylori-Infected Patients. J Immunol. 2016;197:1801-8 pubmed publisher
  • flow cytometry; African green monkey; loading ...; fig 5a
Phuah J, Wong E, Gideon H, Maiello P, Coleman M, Hendricks M, et al. Effects of B Cell Depletion on Early Mycobacterium tuberculosis Infection in Cynomolgus Macaques. Infect Immun. 2016;84:1301-1311 pubmed publisher
  • flow cytometry; human; fig 1
Sharma S, Roumanes D, Almudevar A, Mosmann T, Pichichero M. CD4+ T-cell responses among adults and young children in response to Streptococcus pneumoniae and Haemophilus influenzae vaccine candidate protein antigens. Vaccine. 2013;31:3090-7 pubmed publisher
  • blocking or activating experiments; human
Dolganiuc A, Kodys K, Marshall C, Saha B, Zhang S, Bala S, et al. Type III interferons, IL-28 and IL-29, are increased in chronic HCV infection and induce myeloid dendritic cell-mediated FoxP3+ regulatory T cells. PLoS ONE. 2012;7:e44915 pubmed publisher
  • flow cytometry; rhesus macaque
  • flow cytometry; African green monkey; fig 3
de Campos S, Souza Lemos C, Teva A, Porrozzi R, Grimaldi G. Systemic and compartmentalised immune responses in a Leishmania braziliensis-macaque model of self-healing cutaneous leishmaniasis. Vet Immunol Immunopathol. 2010;137:149-54 pubmed publisher
  • flow cytometry; human; fig 3
Della Bella S, Giannelli S, Taddeo A, Presicce P, Villa M. Application of six-color flow cytometry for the assessment of dendritic cell responses in whole blood assays. J Immunol Methods. 2008;339:153-64 pubmed publisher
  • immunohistochemistry; rhesus macaque
Cumont M, Monceaux V, Viollet L, Lay S, Parker R, Hurtrel B, et al. TGF-beta in intestinal lymphoid organs contributes to the death of armed effector CD8 T cells and is associated with the absence of virus containment in rhesus macaques infected with the simian immunodeficiency virus. Cell Death Differ. 2007;14:1747-58 pubmed publisher
  • blocking or activating experiments; human
Yu Q, Saruta M, Avanesyan A, Fleshner P, Banham A, Papadakis K. Expression and functional characterization of FOXP3+ CD4+ regulatory T cells in ulcerative colitis. Inflamm Bowel Dis. 2007;13:191-9 pubmed
  • immunohistochemistry - paraffin section; human
Uhlig H, Coombes J, Mottet C, Izcue A, Thompson C, Fanger A, et al. Characterization of Foxp3+CD4+CD25+ and IL-10-secreting CD4+CD25+ T cells during cure of colitis. J Immunol. 2006;177:5852-60 pubmed
  • blocking or activating experiments; human; 2.5 ul/10^6 cells
Ohman H, Tiitinen A, Halttunen M, Birkelund S, Christiansen G, Koskela P, et al. IL-10 polymorphism and cell-mediated immune response to Chlamydia trachomatis. Genes Immun. 2006;7:243-9 pubmed
  • blocking or activating experiments; human
Kariminia A, Bourreau E, Pascalis H, Couppie P, Sainte Marie D, Tacchini Cottier F, et al. Transforming growth factor beta 1 production by CD4+ CD25+ regulatory T cells in peripheral blood mononuclear cells from healthy subjects stimulated with Leishmania guyanensis. Infect Immun. 2005;73:5908-14 pubmed
  • flow cytometry; human; fig 2
Kirchberger S, Majdic O, Steinberger P, Bluml S, Pfistershammer K, Zlabinger G, et al. Human rhinoviruses inhibit the accessory function of dendritic cells by inducing sialoadhesin and B7-H1 expression. J Immunol. 2005;175:1145-52 pubmed
  • flow cytometry; human; fig 5
Hatachi S, Iwai Y, Kawano S, Morinobu S, Kobayashi M, Koshiba M, et al. CD4+ PD-1+ T cells accumulate as unique anergic cells in rheumatoid arthritis synovial fluid. J Rheumatol. 2003;30:1410-9 pubmed
  • flow cytometry; human; 5 ug/ml; fig 1
Cousins D, Lee T, Staynov D. Cytokine coexpression during human Th1/Th2 cell differentiation: direct evidence for coordinated expression of Th2 cytokines. J Immunol. 2002;169:2498-506 pubmed
  • flow cytometry; human
Reignat S, Webster G, Brown D, Ogg G, King A, Seneviratne S, et al. Escaping high viral load exhaustion: CD8 cells with altered tetramer binding in chronic hepatitis B virus infection. J Exp Med. 2002;195:1089-101 pubmed
  • flow cytometry; human
Van Damme N, De Vos M, Baeten D, Demetter P, Mielants H, Verbruggen G, et al. Flow cytometric analysis of gut mucosal lymphocytes supports an impaired Th1 cytokine profile in spondyloarthropathy. Ann Rheum Dis. 2001;60:495-9 pubmed
  • ELISA; human; fig 4
Prehn J, Landers C, Targan S. A soluble factor produced by lamina propria mononuclear cells is required for TNF-alpha enhancement of IFN-gamma production by T cells. J Immunol. 1999;163:4277-83 pubmed
  • flow cytometry; human; fig 2
Shimonkevitz R, Bar Or D, Harris L, Dole K, McLaughlin L, Yukl R. Transient monocyte release of interleukin-10 in response to traumatic brain injury. Shock. 1999;12:10-6 pubmed
  • flow cytometry; human
Koshiba M, Rosin D, Hayashi N, Linden J, Sitkovsky M. Patterns of A2A extracellular adenosine receptor expression in different functional subsets of human peripheral T cells. Flow cytometry studies with anti-A2A receptor monoclonal antibodies. Mol Pharmacol. 1999;55:614-24 pubmed
  • blocking or activating experiments; human
Kanto T, Hayashi N, Takehara T, Katayama K, Ito A, Mochizuki K, et al. Cross-linking of Fc(gamma)-receptor on monocytes inhibits hepatitis C virus-specific cytotoxic T-lymphocyte induction in vitro. Immunology. 1998;94:461-8 pubmed
  • blocking or activating experiments; human
Berger S, Chandra R, Ballo H, Hildenbrand R, Stutte H. Immune complexes are potent inhibitors of interleukin-12 secretion by human monocytes. Eur J Immunol. 1997;27:2994-3000 pubmed
Liao X, Zhou S, Zong J, Wang Z. Sevoflurane exerts protective effects on liver ischemia/reperfusion injury by regulating NFKB3 expression via miR-9-5p. Exp Ther Med. 2019;17:2632-2640 pubmed publisher
Chen D, Xie J, Fiskesund R, Dong W, Liang X, Lv J, et al. Chloroquine modulates antitumor immune response by resetting tumor-associated macrophages toward M1 phenotype. Nat Commun. 2018;9:873 pubmed publisher
Roux C, Saviane G, Pini J, Belaïd N, Dhib G, Voha C, et al. Immunosuppressive Mesenchymal Stromal Cells Derived from Human-Induced Pluripotent Stem Cells Induce Human Regulatory T Cells In Vitro and In Vivo. Front Immunol. 2017;8:1991 pubmed publisher
Vargas Hernandez A, Mace E, Zimmerman O, Zerbe C, Freeman A, Rosenzweig S, et al. Ruxolitinib partially reverses functional natural killer cell deficiency in patients with signal transducer and activator of transcription 1 (STAT1) gain-of-function mutations. J Allergy Clin Immunol. 2018;141:2142-2155.e5 pubmed publisher
Powell R, Lissauer D, Tamblyn J, Beggs A, Cox P, Moss P, et al. Decidual T Cells Exhibit a Highly Differentiated Phenotype and Demonstrate Potential Fetal Specificity and a Strong Transcriptional Response to IFN. J Immunol. 2017;199:3406-3417 pubmed publisher
Schwerd T, Twigg S, Aschenbrenner D, Manrique S, Miller K, Taylor I, et al. A biallelic mutation in IL6ST encoding the GP130 co-receptor causes immunodeficiency and craniosynostosis. J Exp Med. 2017;214:2547-2562 pubmed publisher
Sulzer D, Alcalay R, Garretti F, Cote L, Kanter E, Agin Liebes J, et al. T cells from patients with Parkinson's disease recognize α-synuclein peptides. Nature. 2017;546:656-661 pubmed publisher
Thangavadivel S, Zelle Rieser C, Olivier A, Postert B, Untergasser G, Kern J, et al. CCR10/CCL27 crosstalk contributes to failure of proteasome-inhibitors in multiple myeloma. Oncotarget. 2016;7:78605-78618 pubmed publisher
Hutchinson K, Villinger F, Miranda M, Ksiazek T, Peters C, Rollin P. Multiplex analysis of cytokines in the blood of cynomolgus macaques naturally infected with Ebola virus (Reston serotype). J Med Virol. 2001;65:561-6 pubmed publisher
Nixon C, Steffen M, Ebersole J. Cytokine responses to treponema pectinovorum and treponema denticola in human gingival fibroblasts. Infect Immun. 2000;68:5284-92 pubmed
product information
Product Type :
Antibody
Product Name :
IL-10 Monoclonal Antibody (JES3-9D7)
Catalog # :
AHC0103
Quantity :
1 mg
Price :
US 468.00
Clonality :
Monoclonal
Purity :
purified
Host :
Rat
Reactivity :
Human
Applications :
ELISA: Assay-dependent, Flow Cytometry: Assay-dependent, Neutralization: Assay-dependent
Species :
Human
Clone :
JES3-9D7
Isotype :
IgG1, kappa
Storage :
-20°C
Description :
IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.
Immunogen :
Recombinant human Interleukin 10.
Format :
Liquid
Applications w/Dilutions :
ELISA: Assay-dependent, Flow Cytometry: Assay-dependent, Neutralization: Assay-dependent
Aliases :
CSIF; cytokine; Cytokine synthesis inhibitory factor; GVHDS; H-IL-10; il 10; IL10; IL-10; IL-10 precursor; IL10A; IL10X; ILN; Interleukin; interleukin 10; Interleukin10; interleukin-10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor; TGIF
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA