c-Kit Monoclonal Antibody (2B8), APC-Cyanine7 | Invitrogen A15423 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

c-Kit Monoclonal Antibody (2B8), APC-Cyanine7

catalog :

A15423

quantity :

100 ug

price :

US 306.00

clonality :

monoclonal

host :

rat

conjugate :

APC

clone name :

2B8

reactivity :

mouse

application :

flow cytometry

more info or order :

Invitrogen product webpage

citations: 9

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; loading ...; fig 2j	Gopal A, Ibrahim R, Fuller M, Umlandt P, Parker J, Tran J, et al. TIRAP drives myelosuppression through an Ifnγ-Hmgb1 axis that disrupts the endothelial niche in mice. J Exp Med. 2022;219: pubmed publisher
flow cytometry; mouse; 1:200; loading ...; fig e1b	Aslam M, Alemdehy M, Kwesi Maliepaard E, Muhaimin F, Caganova M, Pardieck I, et al. Histone methyltransferase DOT1L controls state-specific identity during B cell differentiation. EMBO Rep. 2021;22:e51184 pubmed publisher
flow cytometry; mouse; loading ...; fig e10	Majer O, Liu B, Kreuk L, Krogan N, Barton G. UNC93B1 recruits syntenin-1 to dampen TLR7 signalling and prevent autoimmunity. Nature. 2019;575:366-370 pubmed publisher
flow cytometry; mouse; loading ...; fig 2d	Guarnerio J, Mendez L, Asada N, Menon A, Fung J, Berry K, et al. A non-cell-autonomous role for Pml in the maintenance of leukemia from the niche. Nat Commun. 2018;9:66 pubmed publisher
flow cytometry; mouse; loading ...	Costa D, Principi E, Lazzarini E, Descalzi F, Cancedda R, Castagnola P, et al. LCN2 overexpression in bone enhances the hematopoietic compartment via modulation of the bone marrow microenvironment. J Cell Physiol. 2017;232:3077-3087 pubmed publisher
flow cytometry; mouse; fig 1b	Marks Bluth J, Khanna A, Chandrakanthan V, Thoms J, Bee T, Eich C, et al. SMAD1 and SMAD5 Expression Is Coordinately Regulated by FLI1 and GATA2 during Endothelial Development. Mol Cell Biol. 2015;35:2165-72 pubmed publisher
	Komuro M, Mizugaki H, Nagane M, Morimoto M, Fukuyama T, Ogihara K, et al. Ganglioside GM3 deficiency enhances mast cell sensitivity. FEBS J. 2023;290:4268-4280 pubmed publisher
	Kumar S, Vassallo J, Nattamai K, Hassan A, Karns R, Vollmer A, et al. pH regulates hematopoietic stem cell potential via polyamines. EMBO Rep. 2023;24:e55373 pubmed publisher
	Habowski A, Flesher J, Bates J, Tsai C, Martin K, Zhao R, et al. Transcriptomic and proteomic signatures of stemness and differentiation in the colon crypt. Commun Biol. 2020;3:453 pubmed publisher

product information

Product Type :

Antibody

Product Name :

c-Kit Monoclonal Antibody (2B8), APC-Cyanine7

Catalog # :

A15423

Quantity :

100 ug

Price :

US 306.00

Clonality :

Monoclonal

Purity :

purified

Host :

Rat

Reactivity :

Mouse

Applications :

Flow Cytometry: 0.2 ug/1x10^6 cells

Species :

Mouse

Clone :

2B8

Isotype :

IgG2b, kappa

Storage :

4 C, store in dark, DO NOT FREEZE!

Description :

KIT (c-KIT) is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). KIT was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT together with its ligand regulates growth and activation of a variety of hemopoietic and non-hemopoietic cells. Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Recently, deregulation of the KIT receptor TK by the prevalent activation loop mutation D816V has served as a focal point in therapeutic strategies aimed at curbing neoplastic mast cell growth. c-Kit is expressed in hematopoietic stem cells, germ cells, mast cells and gastrointestinal tract cajal cells. Upon binding of its ligand stem cell factor (SCF), c-kit dimerizes, resulting in receptor activation and autophosphorylation of various tyrosine residues including tyrosine 703 located on the cytoplasmic domain of the receptor. This modification allows docking of Grb2 and activation of the Ras/ERK signaling pathway. SCF/c-kit can activate multiple downstream signaling pathways including PI3K, PLC-gamma and JAK/STAT. c-kit receptor activation is essential for hematopoiesis, stem cell maintenance and gametogenesis.

Immunogen :

IL-3-dependent mast cells derived from normal bone marrow.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 0.2 ug/1x10^6 cells

Aliases :

belly-spot; Bs; CD117; ckit; C-Kit; c-KIT gene1; c-kit protein; c-kit proto-oncogene protein; c-kit receptor; c-kit receptor tyrosine kinase; dominant spotting; Dominant white spotting; Fdc; Gsfsco1; Gsfsco5; Gsfsow3; KIT; kit oncogene; KIT proto-oncogene receptor tyrosine kinase; KIT proto-oncogene, receptor tyrosine kinase; mast cell growth factor receptor; mast/stem cell growth factor receptor; mast/stem cell growth factor receptor Kit; MGF; p145 c-kit; PBT; Piebald trait protein; protein kinase; proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; receptor tyrosine kinase c-kit; receptor tyrosine kinase type III; SCFR; SCO1; SCO5; Sl; soluble KIT variant 1; SOW3; spotted sterile male; Ssm; Steel Factor Receptor; Tr-kit; tyrosine kinase receptor; tyrosine kinase receptor c-KIT; tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein; W

more info or order :

Invitrogen product webpage