product summary
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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
alpha Tubulin Monoclonal Antibody (236-10501)
catalog :
A11126
quantity :
50 µg
price :
US 376
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
236-10501
reactivity :
Cyrtanthus mackenii, malaria parasite P. falciparum, field poppy, Leishmania , African green monkey, hamsters, kangaroo, human, mouse, rat, bovine, fruit fly , Xenopus laevis
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 143
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - paraffin section; human; fig 5i
Werder R, Liu T, Abo K, Lindstrom Vautrin J, Villacorta Martin C, Huang J, et al. CRISPR interference interrogation of COPD GWAS genes reveals the functional significance of desmoplakin in iPSC-derived alveolar epithelial cells. Sci Adv. 2022;8:eabo6566 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 8d
Sierra Potchanant E, Cerabona D, Sater Z, He Y, Sun Z, Gehlhausen J, et al. INPP5E Preserves Genomic Stability through Regulation of Mitosis. Mol Cell Biol. 2017;37: pubmed publisher
  • immunocytochemistry; mouse; 1:400; loading ...; fig 3a
Hamdan M, Jones K, Cheong Y, Lane S. The sensitivity of the DNA damage checkpoint prevents oocyte maturation in endometriosis. Sci Rep. 2016;6:36994 pubmed publisher
  • immunohistochemistry; fruit fly ; 1:100; loading ...; fig s4
Tofangchi A, Fan A, Saif M. Mechanism of Axonal Contractility in Embryonic Drosophila Motor Neurons In Vivo. Biophys J. 2016;111:1519-1527 pubmed publisher
  • western blot; human; loading ...; fig 5a
Zhuang J, Kamp W, Li J, Liu C, Kang J, Wang P, et al. Forkhead Box O3A (FOXO3) and the Mitochondrial Disulfide Relay Carrier (CHCHD4) Regulate p53 Protein Nuclear Activity in Response to Exercise. J Biol Chem. 2016;291:24819-24827 pubmed
  • immunocytochemistry; mouse; 1:50; fig 7
Busse B, Bezrukov L, Blank P, Zimmerberg J. Resin embedded multicycle imaging (REMI): a tool to evaluate protein domains. Sci Rep. 2016;6:30284 pubmed publisher
  • immunohistochemistry; mouse; 1:200; loading ...; fig 2b
Mihajlovic A, Bruce A. Rho-associated protein kinase regulates subcellular localisation of Angiomotin and Hippo-signalling during preimplantation mouse embryo development. Reprod Biomed Online. 2016;33:381-90 pubmed publisher
  • western blot; human; fig 2
Harwardt T, Lukas S, Zenger M, Reitberger T, Danzer D, Übner T, et al. Human Cytomegalovirus Immediate-Early 1 Protein Rewires Upstream STAT3 to Downstream STAT1 Signaling Switching an IL6-Type to an IFNγ-Like Response. PLoS Pathog. 2016;12:e1005748 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:250; fig s5
Elbaz B, Traka M, Kunjamma R, Dukala D, Brosius Lutz A, Anton E, et al. Adenomatous polyposis coli regulates radial axonal sorting and myelination in the PNS. Development. 2016;143:2356-66 pubmed publisher
  • immunocytochemistry; mouse; 1:400; fig 6
Camlin N, Sobinoff A, Sutherland J, Beckett E, Jarnicki A, Vanders R, et al. Maternal Smoke Exposure Impairs the Long-Term Fertility of Female Offspring in a Murine Model. Biol Reprod. 2016;94:39 pubmed publisher
  • immunocytochemistry; malaria parasite P. falciparum; 1:2000; fig 3
Kono M, Heincke D, Wilcke L, Wong T, Bruns C, Herrmann S, et al. Pellicle formation in the malaria parasite. J Cell Sci. 2016;129:673-80 pubmed publisher
  • immunocytochemistry; human; fig 8
Zhang Z, Wu N, Lu Y, Davidson D, Colonna M, Veillette A. DNAM-1 controls NK cell activation via an ITT-like motif. J Exp Med. 2015;212:2165-82 pubmed publisher
  • immunocytochemistry; human; 1:1000
Cooper S, Sadok A, Bousgouni V, Bakal C. Apolar and polar transitions drive the conversion between amoeboid and mesenchymal shapes in melanoma cells. Mol Biol Cell. 2015;26:4163-70 pubmed publisher
  • immunocytochemistry; human; 1:1000; fig 1
Barr A, Bakal C. A sensitised RNAi screen reveals a ch-TOG genetic interaction network required for spindle assembly. Sci Rep. 2015;5:10564 pubmed publisher
  • immunocytochemistry; mouse
Omari S, Waters M, Naranian T, Kim K, Perumalsamy A, Chi M, et al. Mcl-1 is a key regulator of the ovarian reserve. Cell Death Dis. 2015;6:e1755 pubmed publisher
  • western blot; human
Navis A, van Lith S, van Duijnhoven S, de Pooter M, Yetkin Arik B, Wesseling P, et al. Identification of a novel MET mutation in high-grade glioma resulting in an auto-active intracellular protein. Acta Neuropathol. 2015;130:131-44 pubmed publisher
  • immunocytochemistry; mouse; 1:200
Wu L, Russell D, Wong S, Chen M, Tsai T, St John J, et al. Mitochondrial dysfunction in oocytes of obese mothers: transmission to offspring and reversal by pharmacological endoplasmic reticulum stress inhibitors. Development. 2015;142:681-91 pubmed publisher
  • immunocytochemistry; African green monkey
Ali M, Chuang C, Saif M. Reprogramming cellular phenotype by soft collagen gels. Soft Matter. 2014;10:8829-37 pubmed publisher
  • western blot; mouse
Strickland A, Rebelo A, Zhang F, Price J, Bolon B, Silva J, et al. Characterization of the mitofusin 2 R94W mutation in a knock-in mouse model. J Peripher Nerv Syst. 2014;19:152-64 pubmed publisher
  • immunohistochemistry - paraffin section; human; 0.5 ug/ml
Gu L, Talati P, Vogiatzi P, Romero Weaver A, Abdulghani J, Liao Z, et al. Pharmacologic suppression of JAK1/2 by JAK1/2 inhibitor AZD1480 potently inhibits IL-6-induced experimental prostate cancer metastases formation. Mol Cancer Ther. 2014;13:1246-58 pubmed publisher
  • western blot; bovine; fig 5, 6
Turner M, Cronin J, Healey G, Sheldon I. Epithelial and stromal cells of bovine endometrium have roles in innate immunity and initiate inflammatory responses to bacterial lipopeptides in vitro via Toll-like receptors TLR2, TLR1, and TLR6. Endocrinology. 2014;155:1453-65 pubmed publisher
  • immunocytochemistry; human
Proia D, Zhang C, Sequeira M, Jimenez J, He S, Spector N, et al. Preclinical activity profile and therapeutic efficacy of the HSP90 inhibitor ganetespib in triple-negative breast cancer. Clin Cancer Res. 2014;20:413-24 pubmed publisher
  • western blot; human; fig 4
Tan C, Hagen T. mTORC1 dependent regulation of REDD1 protein stability. PLoS ONE. 2013;8:e63970 pubmed publisher
  • western blot; human; fig 2
Tan C, Hagen T. Destabilization of CDC6 upon DNA damage is dependent on neddylation but independent of Cullin E3 ligases. Int J Biochem Cell Biol. 2013;45:1489-98 pubmed publisher
  • immunocytochemistry; Cyrtanthus mackenii; 1 ug/ml; fig 3
Hirano T, Takagi K, Hoshino Y, Abe T. DNA damage response in male gametes of Cyrtanthus mackenii during pollen tube growth. AoB Plants. 2013;5:plt004 pubmed publisher
  • western blot; human; fig 2
Navis A, Bourgonje A, Wesseling P, Wright A, Hendriks W, Verrijp K, et al. Effects of dual targeting of tumor cells and stroma in human glioblastoma xenografts with a tyrosine kinase inhibitor against c-MET and VEGFR2. PLoS ONE. 2013;8:e58262 pubmed publisher
  • western blot; human; fig 1
Tan C, Hagen T. Post-translational regulation of mTOR complex 1 in hypoxia and reoxygenation. Cell Signal. 2013;25:1235-44 pubmed publisher
  • immunocytochemistry; mouse; 1:200
Lang J, Maeda Y, Bannerman P, Xu J, Horiuchi M, Pleasure D, et al. Adenomatous polyposis coli regulates oligodendroglial development. J Neurosci. 2013;33:3113-30 pubmed publisher
  • western blot; human; fig 1
Greer Y, Fields A, Brown A, Rubin J. Atypical protein kinase C? is required for Wnt3a-dependent neurite outgrowth and binds to phosphorylated dishevelled 2. J Biol Chem. 2013;288:9438-46 pubmed publisher
  • immunocytochemistry; human; 1 ug/ml; fig 5
  • western blot; human; 1:1000; fig s5
Brandhagen B, Tieszen C, Ulmer T, Tracy M, Goyeneche A, Telleria C. Cytostasis and morphological changes induced by mifepristone in human metastatic cancer cells involve cytoskeletal filamentous actin reorganization and impairment of cell adhesion dynamics. BMC Cancer. 2013;13:35 pubmed publisher
  • western blot; mouse; fig 1
  • western blot; human; fig 1
Hu C, Sethi J, Hagen T. The role of the cullin-5 e3 ubiquitin ligase in the regulation of insulin receptor substrate-1. Biochem Res Int. 2012;2012:282648 pubmed publisher
  • immunocytochemistry; bovine; 1:200
Duncan A, Forcina J, Birt A, Townson D. Estrous cycle-dependent changes of Fas expression in the bovine corpus luteum: influence of keratin 8/18 intermediate filaments and cytokines. Reprod Biol Endocrinol. 2012;10:90 pubmed publisher
  • immunocytochemistry; human; 1:200; fig 4
Loessner D, Quent V, Kraemer J, Weber E, Hutmacher D, Magdolen V, et al. Combined expression of KLK4, KLK5, KLK6, and KLK7 by ovarian cancer cells leads to decreased adhesion and paclitaxel-induced chemoresistance. Gynecol Oncol. 2012;127:569-78 pubmed publisher
  • immunocytochemistry; mouse
Holt J, Lane S, Jennings P, Garcia Higuera I, Moreno S, Jones K. APC(FZR1) prevents nondisjunction in mouse oocytes by controlling meiotic spindle assembly timing. Mol Biol Cell. 2012;23:3970-81 pubmed publisher
  • immunocytochemistry; human; fig s7
Gartner S, Liu Y, Natesan S. De novo generation of cells within human nurse macrophages and consequences following HIV-1 infection. PLoS ONE. 2012;7:e40139 pubmed publisher
  • western blot; human
Dimberg L, Dimberg A, Ivarsson K, Fryknäs M, Rickardson L, Tobin G, et al. Stat1 activation attenuates IL-6 induced Stat3 activity but does not alter apoptosis sensitivity in multiple myeloma. BMC Cancer. 2012;12:318 pubmed publisher
  • immunohistochemistry; rat; 1:50; fig 6
Watanabe T, Sakai Y, Koga D, Bochimoto H, Hira Y, Hosaka M, et al. A unique ball-shaped Golgi apparatus in the rat pituitary gonadotrope: its functional implications in relation to the arrangement of the microtubule network. J Histochem Cytochem. 2012;60:588-602 pubmed publisher
  • immunocytochemistry; mouse; 1:400
Lane S, Yun Y, Jones K. Timing of anaphase-promoting complex activation in mouse oocytes is predicted by microtubule-kinetochore attachment but not by bivalent alignment or tension. Development. 2012;139:1947-55 pubmed publisher
  • western blot; human
Beriault D, Haddad O, McCuaig J, Robinson Z, Russell D, Lane E, et al. The mechanical behavior of mutant K14-R125P keratin bundles and networks in NEB-1 keratinocytes. PLoS ONE. 2012;7:e31320 pubmed publisher
  • immunocytochemistry; human; fig 2
Charters G, Stones C, Shelling A, Baguley B, Finlay G. Centrosomal dysregulation in human metastatic melanoma cell lines. Cancer Genet. 2011;204:477-85 pubmed publisher
  • western blot; human; fig 1
Choo Y, Boh B, Lou J, Eng J, Leck Y, Anders B, et al. Characterization of the role of COP9 signalosome in regulating cullin E3 ubiquitin ligase activity. Mol Biol Cell. 2011;22:4706-15 pubmed publisher
  • western blot; human; fig 5b
He H, Yu F, Sun C, Luo Y. CBP/p300 and SIRT1 are involved in transcriptional regulation of S-phase specific histone genes. PLoS ONE. 2011;6:e22088 pubmed publisher
  • western blot; human; fig 4
Vanderperre B, Staskevicius A, Tremblay G, McCoy M, O Neill M, Cashman N, et al. An overlapping reading frame in the PRNP gene encodes a novel polypeptide distinct from the prion protein. FASEB J. 2011;25:2373-86 pubmed publisher
  • western blot; human; fig 1
Boh B, Smith P, Hagen T. Neddylation-induced conformational control regulates cullin RING ligase activity in vivo. J Mol Biol. 2011;409:136-45 pubmed publisher
  • immunocytochemistry; human; fig s1
Stern C, Luoma J, Meitzen J, Mermelstein P. Corticotropin releasing factor-induced CREB activation in striatal neurons occurs via a novel G?? signaling pathway. PLoS ONE. 2011;6:e18114 pubmed publisher
  • immunocytochemistry; mouse; fig 2
Chang H, Jennings P, Stewart J, Verrills N, Jones K. Essential role of protein phosphatase 2A in metaphase II arrest and activation of mouse eggs shown by okadaic acid, dominant negative protein phosphatase 2A, and FTY720. J Biol Chem. 2011;286:14705-12 pubmed publisher
  • western blot; human; fig 1
Chua Y, Boh B, Ponyeam W, Hagen T. Regulation of cullin RING E3 ubiquitin ligases by CAND1 in vivo. PLoS ONE. 2011;6:e16071 pubmed publisher
  • western blot; hamsters; fig 4
Skalski M, Sharma N, Williams K, Kruspe A, Coppolino M. SNARE-mediated membrane traffic is required for focal adhesion kinase signaling and Src-regulated focal adhesion turnover. Biochim Biophys Acta. 2011;1813:148-58 pubmed publisher
  • immunocytochemistry; hamsters; fig 4
  • western blot; hamsters; fig 8
Mire C, White J, Whitt M. A spatio-temporal analysis of matrix protein and nucleocapsid trafficking during vesicular stomatitis virus uncoating. PLoS Pathog. 2010;6:e1000994 pubmed publisher
  • immunocytochemistry; human; fig 5
Richter A, Schagdarsurengin U, Rastetter M, Steinmann K, Dammann R. Protein kinase A-mediated phosphorylation of the RASSF1A tumour suppressor at Serine 203 and regulation of RASSF1A function. Eur J Cancer. 2010;46:2986-95 pubmed publisher
  • western blot; human; fig 1
Leck Y, Choo Y, Tan C, Smith P, Hagen T. Biochemical and cellular effects of inhibiting Nedd8 conjugation. Biochem Biophys Res Commun. 2010;398:588-93 pubmed publisher
  • western blot; human; fig 2
Yu F, Chai T, He H, Hagen T, Luo Y. Thioredoxin-interacting protein (Txnip) gene expression: sensing oxidative phosphorylation status and glycolytic rate. J Biol Chem. 2010;285:25822-30 pubmed publisher
  • immunohistochemistry; Cyrtanthus mackenii; 1 ug/ml; fig 2
Hirano T, Hoshino Y. Sperm dimorphism in terms of nuclear shape and microtubule accumulation in Cyrtanthus mackenii. Sex Plant Reprod. 2010;23:153-62 pubmed publisher
  • western blot; human; fig 1c
Irvine M, Philipsz S, Frausto M, Mijatov B, Gallagher S, Fung C, et al. Amino terminal hydrophobic import signals target the p14(ARF) tumor suppressor to the mitochondria. Cell Cycle. 2010;9:829-39 pubmed
  • immunocytochemistry; mouse; 1:200
Chang H, Minahan K, Merriman J, Jones K. Calmodulin-dependent protein kinase gamma 3 (CamKIIgamma3) mediates the cell cycle resumption of metaphase II eggs in mouse. Development. 2009;136:4077-81 pubmed publisher
  • immunocytochemistry; human; fig 1
Shkarupeta M, Kostrjukova E, Lazarev V, Levitskii S, Basovskii Y, Govorun V. Localization of C. trachomatis Inc proteins in expression of their genes in HeLa cell culture. Bull Exp Biol Med. 2008;146:237-42 pubmed
  • western blot; human; fig 3
Haferkamp S, Becker T, Scurr L, Kefford R, Rizos H. p16INK4a-induced senescence is disabled by melanoma-associated mutations. Aging Cell. 2008;7:733-45 pubmed
  • immunocytochemistry; human; 1:200; fig 7
Kim Y, Park S, Park J. Biomechanical analysis of cancerous and normal cells based on bulge generation in a microfluidic device. Analyst. 2008;133:1432-9 pubmed publisher
  • western blot; mouse; fig 1
Nakaya T, Kawai T, Suzuki T. Regulation of FE65 nuclear translocation and function by amyloid beta-protein precursor in osmotically stressed cells. J Biol Chem. 2008;283:19119-31 pubmed publisher
  • immunohistochemistry - frozen section; rat
Carneiro A, Fragel Madeira L, Silva Neto M, Linden R. A role for CK2 upon interkinetic nuclear migration in the cell cycle of retinal progenitor cells. Dev Neurobiol. 2008;68:620-31 pubmed publisher
  • immunocytochemistry; mouse; 1:40; fig 3
Woost P, Kolb R, Chang C, Finesilver M, Inagami T, Hopfer U. Development of an AT2-deficient proximal tubule cell line for transport studies. In Vitro Cell Dev Biol Anim. 2007;43:352-60 pubmed
  • immunocytochemistry; human; 1:400
Gabet A, Accardi R, Bellopede A, Popp S, Boukamp P, Sylla B, et al. Impairment of the telomere/telomerase system and genomic instability are associated with keratinocyte immortalization induced by the skin human papillomavirus type 38. FASEB J. 2008;22:622-32 pubmed
  • immunocytochemistry; kangaroo; 2 ug/ml
Egner A, Geisler C, von Middendorff C, Bock H, Wenzel D, Medda R, et al. Fluorescence nanoscopy in whole cells by asynchronous localization of photoswitching emitters. Biophys J. 2007;93:3285-90 pubmed
  • immunocytochemistry; human; 1:200; fig 3
Nishiya T, Kajita E, Horinouchi T, Nishimoto A, Miwa S. Distinct roles of TIR and non-TIR regions in the subcellular localization and signaling properties of MyD88. FEBS Lett. 2007;581:3223-9 pubmed
  • western blot; human; tbl 1
Supino R, Favini E, Cuccuru G, Zunino F, Scovassi A. Effect of paclitaxel on intracellular localization of c-Myc and P-c-Myc in prostate carcinoma cell lines. Ann N Y Acad Sci. 2007;1095:175-81 pubmed
  • immunohistochemistry; field poppy; 1:200; fig 8
Gossot O, Geitmann A. Pollen tube growth: coping with mechanical obstacles involves the cytoskeleton. Planta. 2007;226:405-16 pubmed
  • western blot; human; fig 1
Chew E, Poobalasingam T, Hawkey C, Hagen T. Characterization of cullin-based E3 ubiquitin ligases in intact mammalian cells--evidence for cullin dimerization. Cell Signal. 2007;19:1071-80 pubmed
  • western blot; mouse
Wegmüller D, Raineri I, Gross B, Oakeley E, Moroni C. A cassette system to study embryonic stem cell differentiation by inducible RNA interference. Stem Cells. 2007;25:1178-85 pubmed
  • immunocytochemistry; human; 1 ug/ml; fig 1
Henley D, Isbill M, Fernando R, Foster J, Wimalasena J. Paclitaxel induced apoptosis in breast cancer cells requires cell cycle transit but not Cdc2 activity. Cancer Chemother Pharmacol. 2007;59:235-49 pubmed
  • immunocytochemistry; Leishmania ; 1:1000; fig 3
Valdes R, Liu W, Ullman B, Landfear S. Comprehensive examination of charged intramembrane residues in a nucleoside transporter. J Biol Chem. 2006;281:22647-55 pubmed
  • immunocytochemistry; mouse; 1:200; fig 1
Sharma M, Leung L, Brocardo M, Henderson J, Flegg C, Henderson B. Membrane localization of adenomatous polyposis coli protein at cellular protrusions: targeting sequences and regulation by beta-catenin. J Biol Chem. 2006;281:17140-9 pubmed
  • immunocytochemistry; mouse; fig 6
Hao M, Li X, Rizzo M, Rocheleau J, Dawant B, Piston D. Regulation of two insulin granule populations within the reserve pool by distinct calcium sources. J Cell Sci. 2005;118:5873-84 pubmed
  • immunocytochemistry; human; fig 1
Remacle A, Rozanov D, Baciu P, Chekanov A, Golubkov V, Strongin A. The transmembrane domain is essential for the microtubular trafficking of membrane type-1 matrix metalloproteinase (MT1-MMP). J Cell Sci. 2005;118:4975-84 pubmed
  • immunocytochemistry; Xenopus laevis; fig 2
Nascimento A, Roland J, Gelfand V. Pigment cells: a model for the study of organelle transport. Annu Rev Cell Dev Biol. 2003;19:469-91 pubmed
  • western blot; human; 0.4 ug/ml; fig 2
Martin K, Hart C, Liu J, Leung W, Patton W. Simultaneous trichromatic fluorescence detection of proteins on Western blots using an amine-reactive dye in combination with alkaline phosphatase- and horseradish peroxidase-antibody conjugates. Proteomics. 2003;3:1215-27 pubmed
  • western blot; human; fig 6
Ashton A, Ware G, Kaul D, Ware J. Inhibition of tumor necrosis factor alpha-mediated NFkappaB activation and leukocyte adhesion, with enhanced endothelial apoptosis, by G protein-linked receptor (TP) ligands. J Biol Chem. 2003;278:11858-66 pubmed
  • western blot; rat; fig 3
Rohde G, Wenzel D, Haucke V. A phosphatidylinositol (4,5)-bisphosphate binding site within mu2-adaptin regulates clathrin-mediated endocytosis. J Cell Biol. 2002;158:209-14 pubmed
  • immunocytochemistry; African green monkey; 1:250; fig 3
Errico A, Ballabio A, Rugarli E. Spastin, the protein mutated in autosomal dominant hereditary spastic paraplegia, is involved in microtubule dynamics. Hum Mol Genet. 2002;11:153-63 pubmed
  • immunocytochemistry; mouse; 1:20,000; fig 6g
Hernandez Blazquez F, Joazeiro P, Omori Y, Yamasaki H. Control of intracellular movement of connexins by E-cadherin in murine skin papilloma cells. Exp Cell Res. 2001;270:235-47 pubmed
  • immunocytochemistry; mouse
Gramolini A, Belanger G, Jasmin B. Distinct regions in the 3' untranslated region are responsible for targeting and stabilizing utrophin transcripts in skeletal muscle cells. J Cell Biol. 2001;154:1173-83 pubmed
  • immunocytochemistry; hamsters; fig 6
Vogelsberg Ragaglia V, Bruce J, Richter Landsberg C, Zhang B, Hong M, Trojanowski J, et al. Distinct FTDP-17 missense mutations in tau produce tau aggregates and other pathological phenotypes in transfected CHO cells. Mol Biol Cell. 2000;11:4093-104 pubmed
  • western blot; human; 1:20,000
Deloulme J, Assard N, Mbele G, Mangin C, Kuwano R, Baudier J. S100A6 and S100A11 are specific targets of the calcium- and zinc-binding S100B protein in vivo. J Biol Chem. 2000;275:35302-10 pubmed
  • western blot; human; fig 4
Pryde J, Walker A, Rossi A, Hannah S, Haslett C. Temperature-dependent arrest of neutrophil apoptosis. Failure of Bax insertion into mitochondria at 15 degrees C prevents the release of cytochrome c. J Biol Chem. 2000;275:33574-84 pubmed
  • western blot; mouse; 1:500; fig 5
Berggren K, Steinberg T, Lauber W, Carroll J, Lopez M, Chernokalskaya E, et al. A luminescent ruthenium complex for ultrasensitive detection of proteins immobilized on membrane supports. Anal Biochem. 1999;276:129-43 pubmed
  • immunocytochemistry; rat; 1:200; fig 2
  • western blot; rat; 1:12,000; fig 4
Timm S, Titus B, Bernd K, Barroso M. The EF-hand Ca(2+)-binding protein p22 associates with microtubules in an N-myristoylation-dependent manner. Mol Biol Cell. 1999;10:3473-88 pubmed
  • immunohistochemistry; bovine; fig 7
Panchuk Voloshina N, Bishop Stewart J, Bhalgat M, Millard P, Mao F, Leung W, et al. Alexa dyes, a series of new fluorescent dyes that yield exceptionally bright, photostable conjugates. J Histochem Cytochem. 1999;47:1179-88 pubmed
  • flow cytometry; human; 1:10
Koester S, Schlossman S, Zhang C, Decker S, Bolton W. APO2.7 defines a shared apoptotic-necrotic pathway in a breast tumor hypoxia model. Cytometry. 1998;33:324-32 pubmed
  • immunohistochemistry; fruit fly ; fig 4
Hime G, Saint R. Zygotic expression of the pebble locus is required for cytokinesis during the postblastoderm mitoses of Drosophila. Development. 1992;114:165-71 pubmed
Xu L, Sun Z, Xing Z, Liu Y, Zhao H, Tang Z, et al. Cur@SF NPs alleviate Friedreich's ataxia in a mouse model through synergistic iron chelation and antioxidation. J Nanobiotechnology. 2022;20:118 pubmed publisher
Newton S, Kong F, Carlton A, Aguilar C, Parker A, Codner G, et al. Neuroplastin genetically interacts with Cadherin 23 and the encoded isoform Np55 is sufficient for cochlear hair cell function and hearing. PLoS Genet. 2022;18:e1009937 pubmed publisher
Liu B, Hu J, Zhao H, Zhao L, Pan S. MicroRNA-155-5p Contributes to 5-Fluorouracil Resistance Through Down-Regulating TP53INP1 in Oral Squamous Cell Carcinoma. Front Oncol. 2021;11:706095 pubmed publisher
Georgakis S, Gkirtzimanaki K, Papadaki G, Gakiopoulou H, Drakos E, Eloranta M, et al. NETs decorated with bioactive IL-33 infiltrate inflamed tissues and induce IFN-α production in patients with SLE. JCI Insight. 2021;6: pubmed publisher
Miao Y, Qian N, Shi L, Hu F, Min W. 9-Cyanopyronin probe palette for super-multiplexed vibrational imaging. Nat Commun. 2021;12:4518 pubmed publisher
Weidema M, Desar I, Hillebrandt Roeffen M, van Erp A, Masuzawa M, Flucke U, et al. PARP inhibition in UV-associated angiosarcoma preclinical models. J Cancer Res Clin Oncol. 2021;147:2579-2590 pubmed publisher
Alcaraz L, Mallavialle A, David T, Derocq D, Delolme F, Dieryckx C, et al. A 9-kDa matricellular SPARC fragment released by cathepsin D exhibits pro-tumor activity in the triple-negative breast cancer microenvironment. Theranostics. 2021;11:6173-6192 pubmed publisher
Yang F, Pang B, Lai K, Cheung N, Dai J, Zhang W, et al. Discovery of a Novel Specific Inhibitor Targeting Influenza A Virus Nucleoprotein with Pleiotropic Inhibitory Effects on Various Steps of the Viral Life Cycle. J Virol. 2021;95: pubmed publisher
Brunet M, Jacques J, Nassari S, Tyzack G, McGoldrick P, Zinman L, et al. The FUS gene is dual-coding with both proteins contributing to FUS-mediated toxicity. EMBO Rep. 2021;22:e50640 pubmed publisher
Chteinberg E, Wetzels S, Gerritsen W, Temmerman L, van den Oord J, Biessen E, et al. Navitoclax combined with Alpelisib effectively inhibits Merkel cell carcinoma cell growth in vitro. Ther Adv Med Oncol. 2020;12:1758835920975621 pubmed publisher
Wilde B, Kaadige M, Guillen K, Butterfield A, Welm B, Ayer D. Protein synthesis inhibitors stimulate MondoA transcriptional activity by driving an accumulation of glucose 6-phosphate. Cancer Metab. 2020;8:27 pubmed publisher
Dermit M, Dodel M, Lee F, Azman M, Schwenzer H, Jones J, et al. Subcellular mRNA Localization Regulates Ribosome Biogenesis in Migrating Cells. Dev Cell. 2020;55:298-313.e10 pubmed publisher
Dickerson M, Dadi P, Butterworth R, Nakhe A, Graff S, Zaborska K, et al. Tetraspanin-7 regulation of L-type voltage-dependent calcium channels controls pancreatic β-cell insulin secretion. J Physiol. 2020;598:4887-4905 pubmed publisher
Garcia Diaz A, Efe G, Kabra K, Patel A, Lowry E, Shneider N, et al. Standardized reporter systems for purification and imaging of human pluripotent stem cell-derived motor neurons and other cholinergic cells. Neuroscience. 2020;: pubmed publisher
Turro E, Astle W, Megy K, Graf S, Greene D, Shamardina O, et al. Whole-genome sequencing of patients with rare diseases in a national health system. Nature. 2020;583:96-102 pubmed publisher
Velasquez Z, López Osorio S, Pervizaj Oruqaj L, Herold S, Hermosilla C, Taubert A. Besnoitia besnoiti-driven endothelial host cell cycle alteration. Parasitol Res. 2020;119:2563-2577 pubmed publisher
van Erp A, van Houdt L, Hillebrandt Roeffen M, van Bree N, Flucke U, Mentzel T, et al. Olaparib and temozolomide in desmoplastic small round cell tumors: a promising combination in vitro and in vivo. J Cancer Res Clin Oncol. 2020;146:1659-1670 pubmed publisher
Woodhead J, D Souza R, Hedges C, Wan J, Berridge M, Cameron Smith D, et al. High-intensity interval exercise increases humanin, a mitochondrial encoded peptide, in the plasma and muscle of men. J Appl Physiol (1985). 2020;128:1346-1354 pubmed publisher
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product information
Product Type :
Antibody
Product Name :
alpha Tubulin Monoclonal Antibody (236-10501)
Catalog # :
A11126
Quantity :
50 µg
Price :
US 376
Clonality :
Monoclonal
Purity :
purified
Host :
Mouse
Reactivity :
Bovine, Human, Mouse
Applications :
Immunocytochemistry: 1:100-1:500, Immunohistochemistry (Paraffin): 1:20-1:200, Western Blot: 1:1,000-1:5,000
Species :
Bovine, Human, Mouse
Clone :
236-10501
Isotype :
IgG1
Storage :
-20°C
Description :
Alpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules, and this intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The gene that encodes alpha tubulin is highly similar to the mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells.
Immunogen :
Bovine Alpha-Tubulin.
Format :
Lyophilized
Applications w/Dilutions :
Immunocytochemistry: 1:100-1:500, Immunohistochemistry (Paraffin): 1:20-1:200, Western Blot: 1:1,000-1:5,000
Aliases :
&agr-tubulin 84B; 1t; a1t; ALPHA 84C; alpha tubulin; alpha tubulin 84B; alpha[[1]] tubulin; alpha[[1]]-tubulin; alpha1; alpha1 tubulin; alpha1t; alpha1tub; alpha1tub84B; alpha1-tubulin; alpha84B; alphaT; alphat-1; alphatub; alpha-tub; alphaTub1; alphatub84; alphaTUB84B; alpha-tub84B; alphaTub84B-PA; alphatubulin; alpha-tubulin; Alpha-tubulin 1; Alpha-tubulin 2; Alpha-tubulin 3; Alpha-tubulin 3C; alpha-tubulin 3C/D; Alpha-tubulin 3D; Alpha-tubulin 3E; alpha-tubulin 4; Alpha-tubulin 6; alphaTubulin 84B; alpha-Tubulin 84B; alpha-Tubulin at 84B; alpha-tubulin I; Alpha-tubulin II; alpha-tubulin III; Alpha-tubulin isotype M-alpha-1; alpha-tubulin isotype M-alpha-2; Alpha-tubulin isotype M-alpha-4; alpha-tubulin isotype M-alpha-6; alpha-tubulin TUB1; alpha-tubulin ubiquitous; alphaTubulin84B; Alpha-Tubulin84B; ALS22; anon-EST:Liang-1.59; anon-EST:Liang-2.30; AT25469p; aTub84B; a-tub84B; bA408E5.3; B-ALPHA-1; bcm948; BEST:LD32507; cb944; CG1913; CG1913-PA; chr3R:2914276. 2914416; clone 1.59; clone 2.30; Detyrosinated tubulin alpha-1A chain; Detyrosinated tubulin alpha-1B chain; Detyrosinated tubulin alpha-1C chain; Detyrosinated tubulin alpha-3 chain; Detyrosinated tubulin alpha-3C chain; Detyrosinated tubulin alpha-3D chain; Detyrosinated tubulin alpha-3E chain; DM1alpha; Dmel CG1913; Dmel_CG1913; DTA1; fb22g06; FLJ30169; H2 ALPHA; H2-ALPHA; hum-a-tub1; hum-a-tub2; I79_019110; K-ALPHA-1; l(3)84Bd; l(3)g3; lis3; LOC100726777; LOC100731885; LOC100857858; LOC101118307; LOC106557476; M[a]4; M[a]6; ms(3)nc33; nc33; RGD1565476; similar to alpha-tubulin isoform 1; similar to tubulin alpha 1; T; Talpha1; Talpha1 alpha-tubulin; TBA1_DROME; Testis-specific alpha-tubulin; Tub; Tub 84B; TUB1; Tub1C; Tub84B; TUBA1; Tuba-1; tuba1 protein; TUBA1/3/4; Tuba1a; tuba1a.L; tuba1a-a; tuba1a-b; TUBA1B; TUBA1C; tuba1l; tuba1l2; TUBA2; TUBA3; TUBA3C; TUBA3D; TUBA3E; Tuba4; Tuba4a; TUBA5; Tuba6; tuba8.S; tubA84B; tubal2; Tubalpha1; tubulin; tubulin 1 alpha-chain; tubulin 1alpha; tubulin alpha; tubulin alpha 1a; tubulin alpha 1a L homeolog; tubulin alpha 1b; tubulin alpha 1c; tubulin alpha 3; tubulin alpha 3c; tubulin alpha 3e; tubulin alpha 4a; tubulin alpha 6; tubulin alpha 8 S homeolog; tubulin alpha1; tubulin alpha-1 chain; tubulin alpha1 promoter; Tubulin alpha-1A chain; tubulin alpha-1B chain; Tubulin alpha-1C chain; tubulin alpha-1C chain-like; tubulin alpha-2 chain; Tubulin alpha-3 chain; Tubulin alpha-3C chain; tubulin alpha-3C/D chain; Tubulin alpha-3D chain; Tubulin alpha-3E chain; tubulin alpha-4 chain; Tubulin alpha-4A chain; Tubulin alpha-5 chain; tubulin alpha-5 chain-like; tubulin alpha-6 chain; tubulin alpha-8 chain; tubulin alpha-ubiquitous chain; tubulin B-alpha-1; Tubulin H2-alpha; tubulin K-alpha-1; tubulin(alpha1); tubulin, alpha 1; tubulin, alpha 1 (testis specific); tubulin, alpha 1, like; tubulin, alpha 1, like 2; tubulin, alpha 1a; tubulin, alpha 1b; tubulin, alpha 1c; tubulin, alpha 2; tubulin, alpha 3c; tubulin, alpha 3e; tubulin, alpha 4; tubulin, alpha 4a; tubulin, alpha 5; tubulin, alpha 6; tubulin, alpha, brain-specific; tubulin, alpha, ubiquitous; tubulin1alpha; tubulin84B; Tubulin-alpha1; Unknown (protein for MGC:133894); unnamed protein product; wu:fb22g06; wu:fb37a10; XELAEV_18019849mg; XELAEV_18020901mg; YML085C
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA