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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
DPM1 Monoclonal Antibody (5C5A7)
catalog :
A-6429
quantity :
250µg
price :
US 439
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
5C5A7
reactivity :
fission yeast, Saccharomyces cerevisiae S288C, Saccharomycetales, brewer's yeast
application :
western blot, immunocytochemistry, proximity ligation assay
more info or order :
Invitrogen product webpage
citations: 31
Published Application/Species/Sample/DilutionReference
  • proximity ligation assay; brewer's yeast; 1:100; fig 4d
  • immunocytochemistry; brewer's yeast; 1:100; fig 4c
Li J, Fuchs S, Zhang J, Wellford S, Schuldiner M, Wang X. An unrecognized function for COPII components in recruiting the viral replication protein BMV 1a to the perinuclear ER. J Cell Sci. 2016;129:3597-3608 pubmed
  • western blot; Saccharomycetales
Stingele J, Schwarz M, Bloemeke N, Wolf P, Jentsch S. A DNA-dependent protease involved in DNA-protein crosslink repair. Cell. 2014;158:327-338 pubmed publisher
  • western blot; Saccharomycetales; 1:1000; fig 3
Dighe S, Kozminski K. Secretory vesicles deliver Cdc42p to sites of polarized growth in S. cerevisiae. PLoS ONE. 2014;9:e99494 pubmed publisher
  • western blot; Saccharomycetales
Usami Y, Uemura S, Mochizuki T, Morita A, Shishido F, Inokuchi J, et al. Functional mapping and implications of substrate specificity of the yeast high-affinity leucine permease Bap2. Biochim Biophys Acta. 2014;1838:1719-29 pubmed publisher
  • western blot; Saccharomycetales; fig 3
Suzuki K, Nakamura S, Morimoto M, Fujii K, Noda N, Inagaki F, et al. Proteomic profiling of autophagosome cargo in Saccharomyces cerevisiae. PLoS ONE. 2014;9:e91651 pubmed publisher
  • western blot; brewer's yeast; 1:2000
Chen J, Korostyshevsky D, Lee S, Perlstein E. Accumulation of an antidepressant in vesiculogenic membranes of yeast cells triggers autophagy. PLoS ONE. 2012;7:e34024 pubmed publisher
  • western blot; brewer's yeast; 1:1000; fig 1
Zhang J, Diaz A, Mao L, Ahlquist P, Wang X. Host acyl coenzyme A binding protein regulates replication complex assembly and activity of a positive-strand RNA virus. J Virol. 2012;86:5110-21 pubmed publisher
  • western blot; brewer's yeast
Kuravi V, Kurischko C, Puri M, Luca F. Cbk1 kinase and Bck2 control MAP kinase activation and inactivation during heat shock. Mol Biol Cell. 2011;22:4892-907 pubmed publisher
  • western blot; brewer's yeast; fig 3
Finnigan G, Hanson Smith V, Houser B, Park H, Stevens T. The reconstructed ancestral subunit a functions as both V-ATPase isoforms Vph1p and Stv1p in Saccharomyces cerevisiae. Mol Biol Cell. 2011;22:3176-91 pubmed publisher
  • western blot; fission yeast; 1:1000; fig 4
Wang X, Diaz A, Hao L, Gancarz B, den Boon J, Ahlquist P. Intersection of the multivesicular body pathway and lipid homeostasis in RNA replication by a positive-strand RNA virus. J Virol. 2011;85:5494-503 pubmed publisher
  • western blot; brewer's yeast
Chang J, Ruiz V, Vancura A. Purification of yeast membranes and organelles by sucrose density gradient centrifugation. Methods Mol Biol. 2008;457:141-9 pubmed
  • western blot; brewer's yeast
Piwko W, Jentsch S. Proteasome-mediated protein processing by bidirectional degradation initiated from an internal site. Nat Struct Mol Biol. 2006;13:691-7 pubmed
  • western blot; brewer's yeast
Protchenko O, Rodriguez Suarez R, Androphy R, Bussey H, Philpott C. A screen for genes of heme uptake identifies the FLC family required for import of FAD into the endoplasmic reticulum. J Biol Chem. 2006;281:21445-57 pubmed
  • western blot; brewer's yeast; 1:2000; fig 5
Parikh B, Baykal U, Di R, Tumer N. Evidence for retro-translocation of pokeweed antiviral protein from endoplasmic reticulum into cytosol and separation of its activity on ribosomes from its activity on capped RNA. Biochemistry. 2005;44:2478-90 pubmed
  • western blot; brewer's yeast; 1:1000; fig 5A
Floyd D, Geva A, Bruinsma S, Overton M, Blumer K, Baranski T. C5a receptor oligomerization. II. Fluorescence resonance energy transfer studies of a human G protein-coupled receptor expressed in yeast. J Biol Chem. 2003;278:35354-61 pubmed
  • western blot; brewer's yeast; fig 1
Rape M, Hoppe T, Gorr I, Kalocay M, Richly H, Jentsch S. Mobilization of processed, membrane-tethered SPT23 transcription factor by CDC48(UFD1/NPL4), a ubiquitin-selective chaperone. Cell. 2001;107:667-77 pubmed
  • western blot; brewer's yeast; fig 2
Legesse Miller A, Sagiv Y, Glozman R, Elazar Z. Aut7p, a soluble autophagic factor, participates in multiple membrane trafficking processes. J Biol Chem. 2000;275:32966-73 pubmed
  • western blot; brewer's yeast; fig 7
Yun C, Ferea T, Rashford J, Ardon O, Brown P, Botstein D, et al. Desferrioxamine-mediated iron uptake in Saccharomyces cerevisiae. Evidence for two pathways of iron uptake. J Biol Chem. 2000;275:10709-15 pubmed
  • western blot; brewer's yeast; fig 4
Choi J, Lou W, Vancura A. A novel membrane-bound glutathione S-transferase functions in the stationary phase of the yeast Saccharomyces cerevisiae. J Biol Chem. 1998;273:29915-22 pubmed
  • western blot; brewer's yeast; 1:2500
Nass R, Rao R. Novel localization of a Na+/H+ exchanger in a late endosomal compartment of yeast. Implications for vacuole biogenesis. J Biol Chem. 1998;273:21054-60 pubmed
  • western blot; brewer's yeast; 1:500; fig 2
Jackson D, Stevens T. VMA12 encodes a yeast endoplasmic reticulum protein required for vacuolar H+-ATPase assembly. J Biol Chem. 1997;272:25928-34 pubmed
Natarajan N, Foresti O, Wendrich K, Stein A, Carvalho P. Quality Control of Protein Complex Assembly by a Transmembrane Recognition Factor. Mol Cell. 2020;77:108-119.e9 pubmed publisher
Choudhary V, Golani G, Joshi A, Cottier S, Schneiter R, Prinz W, et al. Architecture of Lipid Droplets in Endoplasmic Reticulum Is Determined by Phospholipid Intrinsic Curvature. Curr Biol. 2018;28:915-926.e9 pubmed publisher
Delorme Axford E, Abernathy E, Lennemann N, Bernard A, Ariosa A, Coyne C, et al. The exoribonuclease Xrn1 is a post-transcriptional negative regulator of autophagy. Autophagy. 2018;14:898-912 pubmed publisher
Teixeira V, Johnsen L, Martínez Montañés F, Grippa A, Búxo L, Idrissi F, et al. Regulation of lipid droplets by metabolically controlled Ldo isoforms. J Cell Biol. 2018;217:127-138 pubmed publisher
Popelka H, Damasio A, Hinshaw J, Klionsky D, Ragusa M. Structure and function of yeast Atg20, a sorting nexin that facilitates autophagy induction. Proc Natl Acad Sci U S A. 2017;114:E10112-E10121 pubmed publisher
Smindak R, Heckle L, Chittari S, Hand M, Hyatt D, Mantus G, et al. Lipid-dependent regulation of exocytosis in S. cerevisiae by OSBP homolog (Osh) 4. J Cell Sci. 2017;130:3891-3906 pubmed publisher
Gomes F, Palma F, Barros M, Tsuchida E, Turano H, Alegria T, et al. Proteolytic cleavage by the inner membrane peptidase (IMP) complex or Oct1 peptidase controls the localization of the yeast peroxiredoxin Prx1 to distinct mitochondrial compartments. J Biol Chem. 2017;292:17011-17024 pubmed publisher
Feng Y, Backues S, Baba M, Heo J, Harper J, Klionsky D. Phosphorylation of Atg9 regulates movement to the phagophore assembly site and the rate of autophagosome formation. Autophagy. 2016;12:648-58 pubmed publisher
Nakatogawa H, Ishii J, Asai E, Ohsumi Y. Atg4 recycles inappropriately lipidated Atg8 to promote autophagosome biogenesis. Autophagy. 2012;8:177-86 pubmed publisher
Shi S, Notenboom S, Dumont M, Ballatori N. Identification of human gene products containing Pro-Pro-x-Tyr (PY) motifs that enhance glutathione and endocytotic marker uptake in yeast. Cell Physiol Biochem. 2010;25:293-306 pubmed publisher
product information
Product Type :
Antibody
Product Name :
DPM1 Monoclonal Antibody (5C5A7)
Catalog # :
A-6429
Quantity :
250µg
Price :
US 439
Clonality :
Monoclonal
Purity :
purified
Host :
Mouse
Reactivity :
Yeast
Applications :
Western Blot: Assay Dependent
Species :
Yeast
Clone :
5C5A7
Isotype :
IgG1
Storage :
4° C
Description :
Dolichol-phosphate mannose (Dol-P-Man) serves as a donor of mannosyl residues on the lumenal side of the endoplasmic reticulum (ER). Lack of Dol-P-Man results in defective surface expression of GPI-anchored proteins. Dol-P-Man is synthesized from GDP-mannose and dolichol-phosphate on the cytosolic side of the ER by the enzyme dolichyl-phosphate mannosyltransferase. Human DPM1 lacks a carboxy-terminal transmembrane domain and signal sequence and is regulated by DPM2.
Immunogen :
Yeast Dolichol phosphate mannose synthase
Format :
Liquid
Applications w/Dilutions :
Western Blot: Assay Dependent
Aliases :
AI118379; AI194292; CDGIE; dolichol monophosphate mannose synthase; dolichol-phosphate (beta-D) mannosyltransferase 1; Dolichol-phosphate mannose synthase; Dolichol-phosphate mannose synthase subunit 1; Dolichol-phosphate mannosyltransferase; Dolichol-phosphate mannosyltransferase subunit 1; Dolichyl-phosphate beta-D-mannosyltransferase; Dolichyl-phosphate beta-D-mannosyltransferase subunit 1; dolichyl-phosphate mannosyltransferase 1; dolichyl-phosphate mannosyltransferase polypeptide 1 catalytic subunit; dolichyl-phosphate mannosyltransferase polypeptide 1, catalytic subunit; DPM synthase; DPM synthase complex, catalytic subunit; DPM synthase subunit 1; DPM1; Mannose-P-dolichol synthase; Mannose-P-dolichol synthase subunit 1; MPD synthase; MPD synthase subunit 1; MPDS; OTTMUSP00000017164; P9705.3; RP23-391M18.1; SED3; YPR183W
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA