Carboxypeptidase Y Monoclonal Antibody (10A5B5) | Invitrogen A-6428 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Carboxypeptidase Y Monoclonal Antibody (10A5B5)

catalog :

A-6428

quantity :

250µg

price :

US 435

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

10A5B5

reactivity :

fission yeast, Saccharomycetales, brewer's yeast

application :

western blot, ELISA, immunocytochemistry, immunoprecipitation

more info or order :

Invitrogen product webpage

citations: 45

Published Application/Species/Sample/Dilution	Reference
western blot; brewer's yeast; loading ...; fig 1b	Fanning S, Haque A, Imberdis T, Baru V, Barrasa M, Nuber S, et al. Lipidomic Analysis of α-Synuclein Neurotoxicity Identifies Stearoyl CoA Desaturase as a Target for Parkinson Treatment. Mol Cell. 2019;73:1001-1014.e8 pubmed publisher
western blot; brewer's yeast; fig 1	Pfeiffer A, Stephanowitz H, Krause E, Volkwein C, Hirsch C, Jarosch E, et al. A Complex of Htm1 and the Oxidoreductase Pdi1 Accelerates Degradation of Misfolded Glycoproteins. J Biol Chem. 2016;291:12195-207 pubmed publisher
western blot; brewer's yeast; fig 7	Mallela S, Almeida R, Ejsing C, Conzelmann A. Functions of Ceramide Synthase Paralogs YPR114w and YJR116w of Saccharomyces cerevisiae. PLoS ONE. 2016;11:e0145831 pubmed publisher
immunocytochemistry; fission yeast; 1:200	Rostislavleva K, Soler N, Ohashi Y, Zhang L, Pardon E, Burke J, et al. Structure and flexibility of the endosomal Vps34 complex reveals the basis of its function on membranes. Science. 2015;350:aac7365 pubmed publisher
western blot; brewer's yeast	MÃ¼ller M, Schmidt O, Angelova M, Faserl K, Weys S, Kremser L, et al. The coordinated action of the MVB pathway and autophagy ensures cell survival during starvation. elife. 2015;4:e07736 pubmed publisher
western blot; fission yeast; 1:100; fig S2	de Leon N, Sharifmoghadam M, Hoya M, Curto M, Doncel C, Valdivieso M. Regulation of cell wall synthesis by the clathrin light chain is essential for viability in Schizosaccharomyces pombe. PLoS ONE. 2013;8:e71510 pubmed publisher
western blot; fission yeast; fig 4	Kartner N, Yao Y, Bhargava A, Manolson M. Topology, glycosylation and conformational changes in the membrane domain of the vacuolar H+-ATPase a subunit. J Cell Biochem. 2013;114:1474-87 pubmed publisher
ELISA; Saccharomycetales; 1:1000; fig 7	Chanroj S, Lu Y, Padmanaban S, Nanatani K, Uozumi N, Rao R, et al. Plant-specific cation/H+ exchanger 17 and its homologs are endomembrane K+ transporters with roles in protein sorting. J Biol Chem. 2011;286:33931-41 pubmed publisher
western blot; fission yeast; fig 5	Dokudovskaya S, Waharte F, Schlessinger A, Pieper U, Devos D, Cristea I, et al. A conserved coatomer-related complex containing Sec13 and Seh1 dynamically associates with the vacuole in Saccharomyces cerevisiae. Mol Cell Proteomics. 2011;10:M110.006478 pubmed publisher
western blot; brewer's yeast; fig 3	Cantagrel V, Lefeber D, Ng B, Guan Z, Silhavy J, Bielas S, et al. SRD5A3 is required for converting polyprenol to dolichol and is mutated in a congenital glycosylation disorder. Cell. 2010;142:203-17 pubmed publisher
western blot; brewer's yeast; fig 6	Aguilar P, Fröhlich F, Rehman M, Shales M, Ulitsky I, Olivera Couto A, et al. A plasma-membrane E-MAP reveals links of the eisosome with sphingolipid metabolism and endosomal trafficking. Nat Struct Mol Biol. 2010;17:901-8 pubmed publisher
western blot; brewer's yeast; fig 3	Wang X, Muratani M, Tansey W, Ptashne M. Proteolytic instability and the action of nonclassical transcriptional activators. Curr Biol. 2010;20:868-71 pubmed publisher
western blot; brewer's yeast; 1:1000; fig 2	Strochlic T, Setty T, Sitaram A, Burd C. Grd19/Snx3p functions as a cargo-specific adapter for retromer-dependent endocytic recycling. J Cell Biol. 2007;177:115-25 pubmed
western blot; brewer's yeast; fig 4	Davis Kaplan S, Compton M, Flannery A, Ward D, Kaplan J, Stevens T, et al. PKR1 encodes an assembly factor for the yeast V-type ATPase. J Biol Chem. 2006;281:32025-35 pubmed
immunoprecipitation; brewer's yeast western blot; brewer's yeast	Gauss R, Jarosch E, Sommer T, Hirsch C. A complex of Yos9p and the HRD ligase integrates endoplasmic reticulum quality control into the degradation machinery. Nat Cell Biol. 2006;8:849-54 pubmed
immunoprecipitation; brewer's yeast	Protchenko O, Rodriguez Suarez R, Androphy R, Bussey H, Philpott C. A screen for genes of heme uptake identifies the FLC family required for import of FAD into the endoplasmic reticulum. J Biol Chem. 2006;281:21445-57 pubmed
western blot; brewer's yeast; 1:5000	Muratani M, Kung C, Shokat K, Tansey W. The F box protein Dsg1/Mdm30 is a transcriptional coactivator that stimulates Gal4 turnover and cotranscriptional mRNA processing. Cell. 2005;120:887-99 pubmed
immunoprecipitation; brewer's yeast; fig 3	Fu L, Sztul E. Traffic-independent function of the Sar1p/COPII machinery in proteasomal sorting of the cystic fibrosis transmembrane conductance regulator. J Cell Biol. 2003;160:157-63 pubmed
immunoprecipitation; brewer's yeast; fig 4A	Caldwell S, Hill K, Cooper A. Degradation of endoplasmic reticulum (ER) quality control substrates requires transport between the ER and Golgi. J Biol Chem. 2001;276:23296-303 pubmed
immunoprecipitation; Saccharomycetales; fig 4	Nothwehr S, Ha S, Bruinsma P. Sorting of yeast membrane proteins into an endosome-to-Golgi pathway involves direct interaction of their cytosolic domains with Vps35p. J Cell Biol. 2000;151:297-310 pubmed
western blot; Saccharomycetales; fig 1	Perzov N, Nelson H, Nelson N. Altered distribution of the yeast plasma membrane H+-ATPase as a feature of vacuolar H+-ATPase null mutants. J Biol Chem. 2000;275:40088-95 pubmed
immunocytochemistry; brewer's yeast; fig 3	Conboy M, Cyert M. Luv1p/Rki1p/Tcs3p/Vps54p, a yeast protein that localizes to the late Golgi and early endosome, is required for normal vacuolar morphology. Mol Biol Cell. 2000;11:2429-43 pubmed
western blot; brewer's yeast	Flury I, Benachour A, Conzelmann A. YLL031c belongs to a novel family of membrane proteins involved in the transfer of ethanolaminephosphate onto the core structure of glycosylphosphatidylinositol anchors in yeast. J Biol Chem. 2000;275:24458-65 pubmed
western blot; brewer's yeast; fig 2	Steel G, Harley C, Boyd A, Morgan A. A screen for dominant negative mutants of SEC18 reveals a role for the AAA protein consensus sequence in ATP hydrolysis. Mol Biol Cell. 2000;11:1345-56 pubmed
western blot; brewer's yeast; fig 7	Yun C, Ferea T, Rashford J, Ardon O, Brown P, Botstein D, et al. Desferrioxamine-mediated iron uptake in Saccharomyces cerevisiae. Evidence for two pathways of iron uptake. J Biol Chem. 2000;275:10709-15 pubmed
western blot; brewer's yeast; fig 1	Nakamura N, Yamamoto A, Wada Y, Futai M. Syntaxin 7 mediates endocytic trafficking to late endosomes. J Biol Chem. 2000;275:6523-9 pubmed
immunoprecipitation; brewer's yeast western blot; brewer's yeast	Munn A, Heese Peck A, Stevenson B, Pichler H, Riezman H. Specific sterols required for the internalization step of endocytosis in yeast. Mol Biol Cell. 1999;10:3943-57 pubmed
western blot; brewer's yeast; fig 6	Landolt Marticorena C, Kahr W, Zawarinski P, Correa J, Manolson M. Substrate- and inhibitor-induced conformational changes in the yeast V-ATPase provide evidence for communication between the catalytic and proton-translocating sectors. J Biol Chem. 1999;274:26057-64 pubmed
immunoprecipitation; brewer's yeast	Jørgensen M, Emr S, Winther J. Ligand recognition and domain structure of Vps10p, a vacuolar protein sorting receptor in Saccharomyces cerevisiae. Eur J Biochem. 1999;260:461-9 pubmed
western blot; brewer's yeast	Stefan C, Blumer K. A syntaxin homolog encoded by VAM3 mediates down-regulation of a yeast G protein-coupled receptor. J Biol Chem. 1999;274:1835-41 pubmed
western blot; brewer's yeast; 1:10,000	Bordallo J, Plemper R, Finger A, Wolf D. Der3p/Hrd1p is required for endoplasmic reticulum-associated degradation of misfolded lumenal and integral membrane proteins. Mol Biol Cell. 1998;9:209-22 pubmed
western blot; brewer's yeast; fig 4	Luo W, Chang A. Novel genes involved in endosomal traffic in yeast revealed by suppression of a targeting-defective plasma membrane ATPase mutant. J Cell Biol. 1997;138:731-46 pubmed
immunoprecipitation; brewer's yeast; fig 2	Darsow T, Rieder S, Emr S. A multispecificity syntaxin homologue, Vam3p, essential for autophagic and biosynthetic protein transport to the vacuole. J Cell Biol. 1997;138:517-29 pubmed
immunocytochemistry; brewer's yeast; 20 ug/ml	Roeder A, Shaw J. Vacuole partitioning during meiotic division in yeast. Genetics. 1996;144:445-58 pubmed
immunoprecipitation; brewer's yeast	Cooper A, Stevens T. Vps10p cycles between the late-Golgi and prevacuolar compartments in its function as the sorting receptor for multiple yeast vacuolar hydrolases. J Cell Biol. 1996;133:529-41 pubmed
western blot; fission yeast; fig 7	Siniossoglou S, Wimmer C, Rieger M, Doye V, Tekotte H, Weise C, et al. A novel complex of nucleoporins, which includes Sec13p and a Sec13p homolog, is essential for normal nuclear pores. Cell. 1996;84:265-75 pubmed
immunoprecipitation; brewer's yeast; fig 2A	Piper R, Cooper A, Yang H, Stevens T. VPS27 controls vacuolar and endocytic traffic through a prevacuolar compartment in Saccharomyces cerevisiae. J Cell Biol. 1995;131:603-17 pubmed
	Goodrum J, Lever A, Coody T, Gottschling D, Hughes A. Rsp5 and Mdm30 reshape the mitochondrial network in response to age-induced vacuole stress. Mol Biol Cell. 2019;30:2141-2154 pubmed publisher
	Zhao S, Suda Y, Nakanishi H, Wang N, Yoko o T, Gao X, et al. Yeast Dop1 is required for glycosyltransferase retrieval from the trans-Golgi network. Biochim Biophys Acta Gen Subj. 2019;: pubmed publisher
	MacDonald C, Piper R. Genetic dissection of early endosomal recycling highlights a TORC1-independent role for Rag GTPases. J Cell Biol. 2017;216:3275-3290 pubmed publisher
	Alonso RodrÃguez E, FernÃ¡ndez PiÃ±ar P, SacristÃ¡n Reviriego A, Molina M, MartÃn H. An Analog-sensitive Version of the Protein Kinase Slt2 Allows Identification of Novel Targets of the Yeast Cell Wall Integrity Pathway. J Biol Chem. 2016;291:5461-72 pubmed publisher
	Bean B, Davey M, Snider J, Jessulat M, Deineko V, Tinney M, et al. Rab5-family guanine nucleotide exchange factors bind retromer and promote its recruitment to endosomes. Mol Biol Cell. 2015;26:1119-28 pubmed publisher
	Kanehara K, Cho Y, Lin Y, Chen C, Yu C, Nakamura Y. Arabidopsis DOK1 encodes a functional dolichol kinase involved in reproduction. Plant J. 2015;81:292-303 pubmed publisher
	Tardiff D, Jui N, Khurana V, Tambe M, Thompson M, Chung C, et al. Yeast reveal a "druggable" Rsp5/Nedd4 network that ameliorates ?-synuclein toxicity in neurons. Science. 2013;342:979-83 pubmed publisher
	Nair U, Yen W, Mari M, Cao Y, Xie Z, Baba M, et al. A role for Atg8-PE deconjugation in autophagosome biogenesis. Autophagy. 2012;8:780-93 pubmed publisher

product information

Product Type :

Antibody

Product Name :

Carboxypeptidase Y Monoclonal Antibody (10A5B5)

Catalog # :

A-6428

Quantity :

250µg

Price :

US 435

Clonality :

Monoclonal

Purity :

Ig fraction

Host :

Mouse

Reactivity :

Yeast

Applications :

Immunocytochemistry: 20 µg/mL, Immunofluorescence: 20 µg/mL, Western Blot: 0.25 µg/mL

Species :

Yeast

Clone :

10A5B5

Isotype :

IgG2a, kappa

Storage :

4° C, do not freeze

Description :

Carboxypeptidase Y is Involved in the degradation of small peptides. It digests peptides containing an aliphatic or hydrophobic residue in P1' position, as well as methionine, leucine or phenylalanine in the P1 position of an ester substrate.

Immunogen :

Purified yeast CPY (carboxypeptidase)

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 20 µg/mL, Immunofluorescence: 20 µg/mL, Western Blot: 0.25 µg/mL

Aliases :

Carboxypeptidase Y; cpy1; pcy1

more info or order :

Invitrogen product webpage