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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
VMA2 Monoclonal Antibody (13D11B2)
catalog :
A-6427
quantity :
250µg
price :
US 439
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
13D11B2
reactivity :
fission yeast, Saccharomyces cerevisiae S288C, bovine, brewer's yeast, domestic sheep
application :
western blot, ELISA, immunocytochemistry, immunoprecipitation
more info or order :
Invitrogen product webpage
citations: 44
Published Application/Species/Sample/DilutionReference
  • western blot; brewer's yeast; 1:4000
Renberg R, Yuan X, Samuel T, Miguel D, Hamza I, Andrews N, et al. The Heme Transport Capacity of LHR1 Determines the Extent of Virulence in Leishmania amazonensis. PLoS Negl Trop Dis. 2015;9:e0003804 pubmed publisher
  • western blot; brewer's yeast; fig 3
Finnigan G, Hanson Smith V, Houser B, Park H, Stevens T. The reconstructed ancestral subunit a functions as both V-ATPase isoforms Vph1p and Stv1p in Saccharomyces cerevisiae. Mol Biol Cell. 2011;22:3176-91 pubmed publisher
  • western blot; fission yeast; fig 5
Dokudovskaya S, Waharte F, Schlessinger A, Pieper U, Devos D, Cristea I, et al. A conserved coatomer-related complex containing Sec13 and Seh1 dynamically associates with the vacuole in Saccharomyces cerevisiae. Mol Cell Proteomics. 2011;10:M110.006478 pubmed publisher
  • western blot; brewer's yeast; 1:5000; fig 3
Wolfe D, Padilla Lopez S, Vitiello S, Pearce D. pH-dependent localization of Btn1p in the yeast model for Batten disease. Dis Model Mech. 2011;4:120-5 pubmed publisher
  • western blot; brewer's yeast; fig 7
Ediger B, Melman S, Pappas D, Finch M, Applen J, Parra K. The tether connecting cytosolic (N terminus) and membrane (C terminus) domains of yeast V-ATPase subunit a (Vph1) is required for assembly of V0 subunit d. J Biol Chem. 2009;284:19522-32 pubmed publisher
  • western blot; brewer's yeast; 1:1000
Flannery A, Stevens T. Functional characterization of the N-terminal domain of subunit H (Vma13p) of the yeast vacuolar ATPase. J Biol Chem. 2008;283:29099-108 pubmed publisher
  • immunocytochemistry; brewer's yeast
Hayashi K, Sun Wada G, Wada Y, Nakanishi Matsui M, Futai M. Defective assembly of a hybrid vacuolar H(+)-ATPase containing the mouse testis-specific E1 isoform and yeast subunits. Biochim Biophys Acta. 2008;1777:1370-7 pubmed publisher
  • immunoprecipitation; brewer's yeast; fig 1
Zuo J, Vergara S, Kohno S, Holliday L. Biochemical and functional characterization of the actin-binding activity of the B subunit of yeast vacuolar H+-ATPase. J Exp Biol. 2008;211:1102-8 pubmed publisher
  • western blot; bovine; fig 1b
Albrecht E, Kolisek M, Viergutz T, Zitnan R, Schweigel M. Molecular identification, immunolocalization, and functional activity of a vacuolar-type H(+)-ATPase in bovine rumen epithelium. J Comp Physiol B. 2008;178:285-95 pubmed
  • western blot; brewer's yeast; fig 1b
Jimenez Sanchez M, Cid V, Molina M. Retrophosphorylation of Mkk1 and Mkk2 MAPKKs by the Slt2 MAPK in the yeast cell integrity pathway. J Biol Chem. 2007;282:31174-85 pubmed
  • western blot; brewer's yeast
Ochotny N, Van Vliet A, Chan N, Yao Y, Morel M, Kartner N, et al. Effects of human a3 and a4 mutations that result in osteopetrosis and distal renal tubular acidosis on yeast V-ATPase expression and activity. J Biol Chem. 2006;281:26102-11 pubmed
  • western blot; domestic sheep; fig 7
Etschmann B, Heipertz K, von der Schulenburg A, Schweigel M. A vH+-ATPase is present in cultured sheep ruminal epithelial cells. Am J Physiol Gastrointest Liver Physiol. 2006;291:G1171-9 pubmed
  • western blot; brewer's yeast
Shao E, Forgac M. Involvement of the nonhomologous region of subunit A of the yeast V-ATPase in coupling and in vivo dissociation. J Biol Chem. 2004;279:48663-70 pubmed
  • immunocytochemistry; brewer's yeast; fig 5D
Ishikawa K, Catlett N, Novak J, Tang F, Nau J, Weisman L. Identification of an organelle-specific myosin V receptor. J Cell Biol. 2003;160:887-97 pubmed
  • immunoprecipitation; brewer's yeast; fig 6
Lu M, Vergara S, Zhang L, Holliday L, Aris J, Gluck S. The amino-terminal domain of the E subunit of vacuolar H(+)-ATPase (V-ATPase) interacts with the H subunit and is required for V-ATPase function. J Biol Chem. 2002;277:38409-15 pubmed
  • immunocytochemistry; brewer's yeast; fig 3
Hirata T, Nakamura N, Omote H, Wada Y, Futai M. Regulation and reversibility of vacuolar H(+)-ATPase. J Biol Chem. 2000;275:386-9 pubmed
  • western blot; brewer's yeast; fig 2
Landolt Marticorena C, Kahr W, Zawarinski P, Correa J, Manolson M. Substrate- and inhibitor-induced conformational changes in the yeast V-ATPase provide evidence for communication between the catalytic and proton-translocating sectors. J Biol Chem. 1999;274:26057-64 pubmed
  • western blot; brewer's yeast; fig 3
Harrison M, Murray J, Powell B, Kim Y, Finbow M, Findlay J. Helical interactions and membrane disposition of the 16-kDa proteolipid subunit of the vacuolar H(+)-ATPase analyzed by cysteine replacement mutagenesis. J Biol Chem. 1999;274:25461-70 pubmed
  • western blot; brewer's yeast; 1:1000; fig 1
Jackson D, Stevens T. VMA12 encodes a yeast endoplasmic reticulum protein required for vacuolar H+-ATPase assembly. J Biol Chem. 1997;272:25928-34 pubmed
  • immunocytochemistry; brewer's yeast; fig 5
Rieder S, Banta L, Köhrer K, McCaffery J, Emr S. Multilamellar endosome-like compartment accumulates in the yeast vps28 vacuolar protein sorting mutant. Mol Biol Cell. 1996;7:985-99 pubmed
  • immunocytochemistry; brewer's yeast; 10 ng/ml; fig 3D
Cereghino J, Marcusson E, Emr S. The cytoplasmic tail domain of the vacuolar protein sorting receptor Vps10p and a subset of VPS gene products regulate receptor stability, function, and localization. Mol Biol Cell. 1995;6:1089-102 pubmed
  • immunocytochemistry; brewer's yeast; fig 4C
Piper R, Cooper A, Yang H, Stevens T. VPS27 controls vacuolar and endocytic traffic through a prevacuolar compartment in Saccharomyces cerevisiae. J Cell Biol. 1995;131:603-17 pubmed
  • immunocytochemistry; brewer's yeast; fig 1
Raymond C, Howald Stevenson I, Vater C, Stevens T. Morphological classification of the yeast vacuolar protein sorting mutants: evidence for a prevacuolar compartment in class E vps mutants. Mol Biol Cell. 1992;3:1389-402 pubmed
Szatkowska R, Garcia Albornoz M, Roszkowska K, Holman S, Furmanek E, Hubbard S, et al. Glycolytic flux in Saccharomyces cerevisiae is dependent on RNA polymerase III and its negative regulator Maf1. Biochem J. 2019;476:1053-1082 pubmed publisher
Choudhary V, Golani G, Joshi A, Cottier S, Schneiter R, Prinz W, et al. Architecture of Lipid Droplets in Endoplasmic Reticulum Is Determined by Phospholipid Intrinsic Curvature. Curr Biol. 2018;28:915-926.e9 pubmed publisher
Adamczyk M, Szatkowska R. Low RNA Polymerase III activity results in up regulation of HXT2 glucose transporter independently of glucose signaling and despite changing environment. PLoS ONE. 2017;12:e0185516 pubmed publisher
Rodríguez Peña J, Diez Muñiz S, Bermejo C, Nombela C, Arroyo J. Activation of the yeast cell wall integrity MAPK pathway by zymolyase depends on protease and glucanase activities and requires the mucin-like protein Hkr1 but not Msb2. FEBS Lett. 2013;587:3675-80 pubmed publisher
Liberman R, Cotter K, Baleja J, Forgac M. Structural analysis of the N-terminal domain of subunit a of the yeast vacuolar ATPase (V-ATPase) using accessibility of single cysteine substitutions to chemical modification. J Biol Chem. 2013;288:22798-808 pubmed publisher
Liu J, Huang X, Withers B, BLALOCK E, Liu K, Dickson R. Reducing sphingolipid synthesis orchestrates global changes to extend yeast lifespan. Aging Cell. 2013;12:833-41 pubmed publisher
Huang X, Liu J, Withers B, Samide A, Leggas M, Dickson R. Reducing signs of aging and increasing lifespan by drug synergy. Aging Cell. 2013;12:652-60 pubmed publisher
Kartner N, Yao Y, Bhargava A, Manolson M. Topology, glycosylation and conformational changes in the membrane domain of the vacuolar H+-ATPase a subunit. J Cell Biochem. 2013;114:1474-87 pubmed publisher
Padilla Lopez S, Langager D, Chan C, Pearce D. BTN1, the Saccharomyces cerevisiae homolog to the human Batten disease gene, is involved in phospholipid distribution. Dis Model Mech. 2012;5:191-9 pubmed publisher
Toei M, Toei S, Forgac M. Definition of membrane topology and identification of residues important for transport in subunit a of the vacuolar ATPase. J Biol Chem. 2011;286:35176-86 pubmed publisher
Lu M, Sautin Y, Holliday L, Gluck S. The glycolytic enzyme aldolase mediates assembly, expression, and activity of vacuolar H+-ATPase. J Biol Chem. 2004;279:8732-9 pubmed
Ferreira T, Mason A, Pypaert M, Allen K, Slayman C. Quality control in the yeast secretory pathway: a misfolded PMA1 H+-ATPase reveals two checkpoints. J Biol Chem. 2002;277:21027-40 pubmed
Massaad M, Herscovics A. Interaction of the endoplasmic reticulum alpha 1,2-mannosidase Mns1p with Rer1p using the split-ubiquitin system. J Cell Sci. 2001;114:4629-35 pubmed
Ha S, Bunch J, Hama H, DeWald D, Nothwehr S. A novel mechanism for localizing membrane proteins to yeast trans-Golgi network requires function of synaptojanin-like protein. Mol Biol Cell. 2001;12:3175-90 pubmed
Kawasaki Nishi S, Nishi T, Forgac M. Arg-735 of the 100-kDa subunit a of the yeast V-ATPase is essential for proton translocation. Proc Natl Acad Sci U S A. 2001;98:12397-402 pubmed
Kawasaki Nishi S, Bowers K, Nishi T, Forgac M, Stevens T. The amino-terminal domain of the vacuolar proton-translocating ATPase a subunit controls targeting and in vivo dissociation, and the carboxyl-terminal domain affects coupling of proton transport and ATP hydrolysis. J Biol Chem. 2001;276:47411-20 pubmed
Nothwehr S, Ha S, Bruinsma P. Sorting of yeast membrane proteins into an endosome-to-Golgi pathway involves direct interaction of their cytosolic domains with Vps35p. J Cell Biol. 2000;151:297-310 pubmed
Vasilyeva E, Liu Q, MacLeod K, Baleja J, Forgac M. Cysteine scanning mutagenesis of the noncatalytic nucleotide binding site of the yeast V-ATPase. J Biol Chem. 2000;275:255-60 pubmed
Urbanowski J, Piper R. The iron transporter Fth1p forms a complex with the Fet5 iron oxidase and resides on the vacuolar membrane. J Biol Chem. 1999;274:38061-70 pubmed
Spelbrink R, Nothwehr S. The yeast GRD20 gene is required for protein sorting in the trans-Golgi network/endosomal system and for polarization of the actin cytoskeleton. Mol Biol Cell. 1999;10:4263-81 pubmed
Nothwehr S, Bruinsma P, Strawn L. Distinct domains within Vps35p mediate the retrieval of two different cargo proteins from the yeast prevacuolar/endosomal compartment. Mol Biol Cell. 1999;10:875-90 pubmed
product information
Product Type :
Antibody
Product Name :
VMA2 Monoclonal Antibody (13D11B2)
Catalog # :
A-6427
Quantity :
250µg
Price :
US 439
Clonality :
Monoclonal
Purity :
purified
Host :
Mouse
Reactivity :
Yeast
Applications :
ELISA: Assay Dependent, Immunocytochemistry: 20 µg/mL, Immunofluorescence: 20 µg/mL, Western Blot: 0.25 µg/mL
Species :
Yeast
Clone :
13D11B2
Isotype :
IgG1
Storage :
4° C
Description :
Vacuolar-type H+-ATPase (V-ATPase) is a multisubunit enzyme responsible for acidification of eukaryotic intracellular organelles. V-ATPases pump protons against an electrochemical gradient, while F-ATPases reverse the process, thereby synthesizing ATP. A peripheral V1 domain, which is responsible for ATP hydrolysis, and a integral V0 domain, which is responsible for proton translocation, compose V-ATPase. Nine subunits (A-H) make up the V1 domain and five subunits (a, d, c, c' and c") make up the V0 domain. Like F-ATPase, V-ATPase most likely operates through a rotary mechanism. The V-ATPase V1 B subunit exists as two isoforms. In the inner ear, the V-ATPase B1 isoform functions in proton secretion and is required to maintain proper endolymph pH and normal auditory function. The gene encoding the human V-ATPase B1 isoform maps to chromosome 2cen-q13. Mutations in this gene cause distal renal tubular acidosis associated with sensorineural deafness. The V-ATPase B2 isoform is expressed in kidney and is the only B isoform expressed in osteoclasts.
Immunogen :
Purified S. cerevisiae vacuole membranes
Format :
Liquid
Applications w/Dilutions :
ELISA: Assay Dependent, Immunocytochemistry: 20 µg/mL, Immunofluorescence: 20 µg/mL, Western Blot: 0.25 µg/mL
Aliases :
ATP6B1; ATP6V1B1; ATPase H+ transporting V1 subunit B1; ATPase, H transporting, lysosomal V1 subunit B1; ATPase, H+ transporting, lysosomal (vacuolar proton pump), beta 56/58 kDa, isoform 1; ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B, isoform 1; ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1; ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1 (Renal tubular acidosis with deafness); ATPase, H+ transporting, lysosomal V1 subunit B1; ATPase, H+ transporting, V1 subunit B, isoform 1; ATPase, H+ transporting, V1 subunit B1; AW208839; BOS_11418; D630003L15; D630030L16Rik; D630039P21Rik; Endomembrane proton pump 58 kDa subunit; H(+)-transporting two-sector ATPase, 58kD subunit; H+-ATPase beta 1 subunit; lysosomal 56/58kDa; RTA1B; vacuolar H+-ATPase; vacuolar proton pump 3; Vacuolar proton pump subunit B 1; vacuolar proton pump, subunit 3; VATB; V-ATPase B1; V-ATPase B1 subunit; V-ATPase subunit B 1; VMA2; VPP3; Vpp-3; V-type proton ATPase subunit B, kidney isoform
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA