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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
MTCO2 Monoclonal Antibody (12C4F12)
catalog :
A-6404
quantity :
100 µg
price :
US 399.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
12C4F12
reactivity :
African green monkey, human, mouse, rat, chicken, bovine
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, other
more info or order :
Invitrogen product webpage
citations: 71
Published Application/Species/Sample/DilutionReference
  • western blot; human; loading ...; fig 2c
Rozanska A, Richter Dennerlein R, Rorbach J, Gao F, Lewis R, Chrzanowska Lightowlers Z, et al. The human RNA-binding protein RBFA promotes the maturation of the mitochondrial ribosome. Biochem J. 2017;474:2145-2158 pubmed publisher
  • immunocytochemistry; human; 1:500; fig 4d
Dai D, Danoviz M, Wiczer B, Laflamme M, Tian R. Mitochondrial Maturation in Human Pluripotent Stem Cell Derived Cardiomyocytes. Stem Cells Int. 2017;2017:5153625 pubmed publisher
  • western blot; human; loading ...; fig 5a
Lu Y, Acoba M, Selvaraju K, Huang T, Nirujogi R, Sathe G, et al. Human adenine nucleotide translocases physically and functionally interact with respirasomes. Mol Biol Cell. 2017;28:1489-1506 pubmed publisher
  • western blot; human; loading ...; fig 3a
Moren C, Gonzalez Casacuberta I, Alvarez Fernández C, Bano M, Catalán García M, Guitart Mampel M, et al. HIV-1 promonocytic and lymphoid cell lines: an in vitro model of in vivo mitochondrial and apoptotic lesion. J Cell Mol Med. 2017;21:402-409 pubmed publisher
  • western blot; human; loading ...; fig 4f
Stroud D, Surgenor E, Formosa L, Reljic B, Frazier A, Dibley M, et al. Accessory subunits are integral for assembly and function of human mitochondrial complex I. Nature. 2016;538:123-126 pubmed publisher
  • western blot; human; loading ...; fig 3d
Maughan R, Feeney E, Capel E, Capeau J, Domingo P, Giralt M, et al. Improved adipose tissue function with initiation of protease inhibitor-only ART. J Antimicrob Chemother. 2016;71:3212-3221 pubmed
  • western blot; human; loading ...
Yen H, Liu Y, Kan C, Wei H, Lee S, Wei Y, et al. Disruption of the human COQ5-containing protein complex is associated with diminished coenzyme Q10 levels under two different conditions of mitochondrial energy deficiency. Biochim Biophys Acta. 2016;1860:1864-76 pubmed publisher
  • western blot; mouse; fig 2
Molla B, Riveiro F, Bolinches Amorós A, Muñoz Lasso D, Palau F, Gonzalez Cabo P. Two different pathogenic mechanisms, dying-back axonal neuropathy and pancreatic senescence, are present in the YG8R mouse model of Friedreich's ataxia. Dis Model Mech. 2016;9:647-57 pubmed publisher
  • western blot; human; 1:10,000; fig 3
Leucci E, Vendramin R, Spinazzi M, Laurette P, Fiers M, Wouters J, et al. Melanoma addiction to the long non-coding RNA SAMMSON. Nature. 2016;531:518-22 pubmed publisher
  • western blot; mouse; fig 6
Suliman H, Zobi F, Piantadosi C. Heme Oxygenase-1/Carbon Monoxide System and Embryonic Stem Cell Differentiation and Maturation into Cardiomyocytes. Antioxid Redox Signal. 2016;24:345-60 pubmed publisher
  • western blot; human; fig 7
Martínez Zamora A, Meseguer S, Esteve J, Villarroya M, Aguado C, Enríquez J, et al. Defective Expression of the Mitochondrial-tRNA Modifying Enzyme GTPBP3 Triggers AMPK-Mediated Adaptive Responses Involving Complex I Assembly Factors, Uncoupling Protein 2, and the Mitochondrial Pyruvate Carrier. PLoS ONE. 2015;10:e0144273 pubmed publisher
  • western blot; human; fig 2
Lauková J, Kozubík A, Hofmanová J, Nekvindová J, Sova P, Moyer M, et al. Loss of PTEN Facilitates Rosiglitazone-Mediated Enhancement of Platinum(IV) Complex LA-12-Induced Apoptosis in Colon Cancer Cells. PLoS ONE. 2015;10:e0141020 pubmed publisher
  • western blot; human; fig 3
Sakabe I, Hu R, Jin L, Clarke R, Kasid U. TMEM33: a new stress-inducible endoplasmic reticulum transmembrane protein and modulator of the unfolded protein response signaling. Breast Cancer Res Treat. 2015;153:285-97 pubmed publisher
  • western blot; human
Lee Y, Jee B, Kwon S, Yoon Y, Xu W, Wang H, et al. Identification of a mitochondrial defect gene signature reveals NUPR1 as a key regulator of liver cancer progression. Hepatology. 2015;62:1174-89 pubmed publisher
  • western blot; human
Lee J, Lee Y, Lim J, Byun H, Park I, Kim G, et al. Mitochondrial Respiratory Dysfunction Induces Claudin-1 Expression via Reactive Oxygen Species-mediated Heat Shock Factor 1 Activation, Leading to Hepatoma Cell Invasiveness. J Biol Chem. 2015;290:21421-31 pubmed publisher
  • western blot; human
Oláhová M, Haack T, Alston C, Houghton J, He L, Morris A, et al. A truncating PET100 variant causing fatal infantile lactic acidosis and isolated cytochrome c oxidase deficiency. Eur J Hum Genet. 2015;23:935-9 pubmed publisher
  • western blot; human; fig 3
Geserick P, Wang J, Feoktistova M, Leverkus M. The ratio of Mcl-1 and Noxa determines ABT737 resistance in squamous cell carcinoma of the skin. Cell Death Dis. 2014;5:e1412 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:1000
Selfridge J, Wilkins H, E L, Carl S, Koppel S, Funk E, et al. Effect of one month duration ketogenic and non-ketogenic high fat diets on mouse brain bioenergetic infrastructure. J Bioenerg Biomembr. 2015;47:1-11 pubmed publisher
  • western blot; mouse; 1:1000
Ribeiro M, Rosenstock T, Oliveira A, Oliveira C, Rego A. Insulin and IGF-1 improve mitochondrial function in a PI-3K/Akt-dependent manner and reduce mitochondrial generation of reactive oxygen species in Huntington's disease knock-in striatal cells. Free Radic Biol Med. 2014;74:129-44 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:100
Garone C, García Diaz B, Emmanuele V, Lopez L, Tadesse S, Akman H, et al. Deoxypyrimidine monophosphate bypass therapy for thymidine kinase 2 deficiency. EMBO Mol Med. 2014;6:1016-27 pubmed publisher
  • western blot; rat
Das S, Bedja D, Campbell N, Dunkerly B, Chenna V, Maitra A, et al. miR-181c regulates the mitochondrial genome, bioenergetics, and propensity for heart failure in vivo. PLoS ONE. 2014;9:e96820 pubmed publisher
  • western blot; mouse
Wolff N, Ghio A, Garrick L, Garrick M, Zhao L, Fenton R, et al. Evidence for mitochondrial localization of divalent metal transporter 1 (DMT1). FASEB J. 2014;28:2134-45 pubmed publisher
  • western blot; human
Ngamsiri P, Watcharasit P, Satayavivad J. Glycogen synthase kinase-3 (GSK3) controls deoxyglucose-induced mitochondrial biogenesis in human neuroblastoma SH-SY5Y cells. Mitochondrion. 2014;14:54-63 pubmed publisher
  • western blot; human
Almalki A, Alston C, Parker A, Simonic I, Mehta S, He L, et al. Mutation of the human mitochondrial phenylalanine-tRNA synthetase causes infantile-onset epilepsy and cytochrome c oxidase deficiency. Biochim Biophys Acta. 2014;1842:56-64 pubmed publisher
  • western blot; human; fig 6
Guo W, Shiina I, Wang Y, Umeda E, Watanabe C, Uetake S, et al. Ridaifen-SB8, a novel tamoxifen derivative, induces apoptosis via reactive oxygen species-dependent signaling pathway. Biochem Pharmacol. 2013;86:1272-84 pubmed publisher
  • western blot; human; fig 3
Nyfeler B, Hoepfner D, Palestrant D, Kirby C, Whitehead L, Yu R, et al. Identification of elongation factor G as the conserved cellular target of argyrin B. PLoS ONE. 2012;7:e42657 pubmed publisher
  • western blot; human; fig 2
Len A, Powner M, Zhu L, Hageman G, Song X, Fruttiger M, et al. Pilot application of iTRAQ to the retinal disease Macular Telangiectasia. J Proteome Res. 2012;11:537-53 pubmed publisher
  • western blot; human; fig 2
Tucker E, Hershman S, Köhrer C, Belcher Timme C, Patel J, Goldberger O, et al. Mutations in MTFMT underlie a human disorder of formylation causing impaired mitochondrial translation. Cell Metab. 2011;14:428-34 pubmed publisher
  • western blot; chicken; fig 1
Solomonia R, Kunelauri N, Mikautadze E, Apkhazava D, McCabe B, Horn G. Mitochondrial proteins, learning and memory: biochemical specialization of a memory system. Neuroscience. 2011;194:112-23 pubmed publisher
  • immunocytochemistry; human; fig 3
Tchakarska G, Roussel M, Troussard X, Sola B. Cyclin D1 inhibits mitochondrial activity in B cells. Cancer Res. 2011;71:1690-9 pubmed publisher
  • western blot; human; fig 4
Dennerlein S, Rozanska A, Wydro M, Chrzanowska Lightowlers Z, Lightowlers R. Human ERAL1 is a mitochondrial RNA chaperone involved in the assembly of the 28S small mitochondrial ribosomal subunit. Biochem J. 2010;430:551-8 pubmed publisher
  • western blot; human
Yee K, Wilkinson S, James J, Ryan K, Vousden K. PUMA- and Bax-induced autophagy contributes to apoptosis. Cell Death Differ. 2009;16:1135-45 pubmed publisher
  • western blot; human
Villarroya J, de Bolos C, Meseguer A, Hirano M, Vila M. Altered gene transcription profiles in fibroblasts harboring either TK2 or DGUOK mutations indicate compensatory mechanisms. Exp Cell Res. 2009;315:1429-38 pubmed publisher
  • western blot; rat
Ungvari Z, Labinskyy N, Gupte S, Chander P, Edwards J, Csiszar A. Dysregulation of mitochondrial biogenesis in vascular endothelial and smooth muscle cells of aged rats. Am J Physiol Heart Circ Physiol. 2008;294:H2121-8 pubmed publisher
  • western blot; human; 1:2000
Balasubramanian S, Ramos J, Luo W, Sirisawad M, Verner E, Buggy J. A novel histone deacetylase 8 (HDAC8)-specific inhibitor PCI-34051 induces apoptosis in T-cell lymphomas. Leukemia. 2008;22:1026-34 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:200
  • immunohistochemistry - frozen section; African green monkey; 1:100
Horn A, Eberhorn A, Hartig W, Ardeleanu P, Messoudi A, Büttner Ennever J. Perioculomotor cell groups in monkey and man defined by their histochemical and functional properties: reappraisal of the Edinger-Westphal nucleus. J Comp Neurol. 2008;507:1317-35 pubmed publisher
  • western blot; mouse; fig 1b
Cho S, Shin E, Park P, Shin D, Chang H, Kim D, et al. Identification of mouse Prp19p as a lipid droplet-associated protein and its possible involvement in the biogenesis of lipid droplets. J Biol Chem. 2007;282:2456-65 pubmed
  • western blot; human
Mazzanti R, Giulivi C. Coordination of nuclear- and mitochondrial-DNA encoded proteins in cancer and normal colon tissues. Biochim Biophys Acta. 2006;1757:618-23 pubmed
  • western blot; human; fig 2
Mazzanti R, Solazzo M, Fantappiè O, Elfering S, Pantaleo P, Bechi P, et al. Differential expression proteomics of human colon cancer. Am J Physiol Gastrointest Liver Physiol. 2006;290:G1329-38 pubmed
  • western blot; human
Clohessy J, Zhuang J, de Boer J, Gil Gomez G, Brady H. Mcl-1 interacts with truncated Bid and inhibits its induction of cytochrome c release and its role in receptor-mediated apoptosis. J Biol Chem. 2006;281:5750-9 pubmed
  • immunohistochemistry; rat; fig 2
Sadacharan S, Singh B, Bowes T, Gupta R. Localization of mitochondrial DNA encoded cytochrome c oxidase subunits I and II in rat pancreatic zymogen granules and pituitary growth hormone granules. Histochem Cell Biol. 2005;124:409-21 pubmed
  • western blot; human; fig 4
Gianni P, Jan K, Douglas M, Stuart P, Tarnopolsky M. Oxidative stress and the mitochondrial theory of aging in human skeletal muscle. Exp Gerontol. 2004;39:1391-400 pubmed
  • western blot; mouse
Cheung N, Choy M, Halliwell B, Teo T, Bay B, Lee A, et al. Lactacystin-induced apoptosis of cultured mouse cortical neurons is associated with accumulation of PTEN in the detergent-resistant membrane fraction. Cell Mol Life Sci. 2004;61:1926-34 pubmed
  • western blot; human
Guo F, Sigua C, Tao J, Bali P, George P, Li Y, et al. Cotreatment with histone deacetylase inhibitor LAQ824 enhances Apo-2L/tumor necrosis factor-related apoptosis inducing ligand-induced death inducing signaling complex activity and apoptosis of human acute leukemia cells. Cancer Res. 2004;64:2580-9 pubmed
  • western blot; human; fig 10
Gajate C, An F, Mollinedo F. Differential cytostatic and apoptotic effects of ecteinascidin-743 in cancer cells. Transcription-dependent cell cycle arrest and transcription-independent JNK and mitochondrial mediated apoptosis. J Biol Chem. 2002;277:41580-9 pubmed
  • western blot; human
Rokhlin O, Glover R, Taghiyev A, Guseva N, Seftor R, Shyshynova I, et al. Bisindolylmaleimide IX facilitates tumor necrosis factor receptor family-mediated cell death and acts as an inhibitor of transcription. J Biol Chem. 2002;277:33213-9 pubmed
  • other; human; fig 3
Esposti M, Erler J, Hickman J, Dive C. Bid, a widely expressed proapoptotic protein of the Bcl-2 family, displays lipid transfer activity. Mol Cell Biol. 2001;21:7268-76 pubmed
  • western blot; rat
Rodrigues C, Stieers C, Keene C, Ma X, Kren B, Low W, et al. Tauroursodeoxycholic acid partially prevents apoptosis induced by 3-nitropropionic acid: evidence for a mitochondrial pathway independent of the permeability transition. J Neurochem. 2000;75:2368-79 pubmed
  • western blot; human
Narvaez C, Welsh J. Role of mitochondria and caspases in vitamin D-mediated apoptosis of MCF-7 breast cancer cells. J Biol Chem. 2001;276:9101-7 pubmed
  • immunocytochemistry; African green monkey; fig 5
Bhagwat S, Biswas G, Anandatheerthavarada H, Addya S, Pandak W, Avadhani N. Dual targeting property of the N-terminal signal sequence of P4501A1. Targeting of heterologous proteins to endoplasmic reticulum and mitochondria. J Biol Chem. 1999;274:24014-22 pubmed
  • western blot; bovine
  • western blot; human
Capaldi R, Marusich M, Taanman J. Mammalian cytochrome-c oxidase: characterization of enzyme and immunological detection of subunits in tissue extracts and whole cells. Methods Enzymol. 1995;260:117-32 pubmed
Seo J, Hwang C, Yoo J. MTFMT deficiency correlates with reduced mitochondrial integrity and enhanced host susceptibility to intracellular infection. Sci Rep. 2020;10:11183 pubmed publisher
Hong S, Lee Y, Park I, Kwon S, Min S, Yoon G. Lactic acidosis caused by repressed lactate dehydrogenase subunit B expression down-regulates mitochondrial oxidative phosphorylation via the pyruvate dehydrogenase (PDH)-PDH kinase axis. J Biol Chem. 2019;294:7810-7820 pubmed publisher
Thomas H, Zhang Y, Stefely J, Veiga S, Thomas G, Kozma S, et al. Mitochondrial Complex I Activity Is Required for Maximal Autophagy. Cell Rep. 2018;24:2404-2417.e8 pubmed publisher
Hawk M, Gorsuch C, Fagan P, Lee C, Kim S, Hamann J, et al. RIPK1-mediated induction of mitophagy compromises the viability of extracellular-matrix-detached cells. Nat Cell Biol. 2018;20:272-284 pubmed publisher
El Merhie N, Baumgart Vogt E, Pilatz A, Pfreimer S, Pfeiffer B, Pak O, et al. Differential Alterations of the Mitochondrial Morphology and Respiratory Chain Complexes during Postnatal Development of the Mouse Lung. Oxid Med Cell Longev. 2017;2017:9169146 pubmed publisher
Tong M, Johansson C, Xiao F, Stone P, James J, Chen Q, et al. Antiphospholipid antibodies increase the levels of mitochondrial DNA in placental extracellular vesicles: Alarmin-g for preeclampsia. Sci Rep. 2017;7:16556 pubmed publisher
Kuntz E, Baquero P, Michie A, Dunn K, Tardito S, Holyoake T, et al. Targeting mitochondrial oxidative phosphorylation eradicates therapy-resistant chronic myeloid leukemia stem cells. Nat Med. 2017;23:1234-1240 pubmed publisher
Parry L, Young M, el Marjou F, Clarke A. Protocols for Analyzing the Role of Paneth Cells in Regenerating the Murine Intestine using Conditional Cre-lox Mouse Models. J Vis Exp. 2015;: pubmed publisher
Ripolone M, Ronchi D, Violano R, Vallejo D, Fagiolari G, Barca E, et al. Impaired Muscle Mitochondrial Biogenesis and Myogenesis in Spinal Muscular Atrophy. JAMA Neurol. 2015;72:666-75 pubmed publisher
Ussakli C, Ebaee A, Binkley J, Brentnall T, Emond M, Rabinovitch P, et al. Mitochondria and tumor progression in ulcerative colitis. J Natl Cancer Inst. 2013;105:1239-48 pubmed publisher
Bereziat V, Cervera P, Le Dour C, Verpont M, Dumont S, Vantyghem M, et al. LMNA mutations induce a non-inflammatory fibrosis and a brown fat-like dystrophy of enlarged cervical adipose tissue. Am J Pathol. 2011;179:2443-53 pubmed publisher
Caron M, Auclair M, Donadille B, Bereziat V, Guerci B, Laville M, et al. Human lipodystrophies linked to mutations in A-type lamins and to HIV protease inhibitor therapy are both associated with prelamin A accumulation, oxidative stress and premature cellular senescence. Cell Death Differ. 2007;14:1759-67 pubmed
Jiang B, Xiao W, Shi Y, Liu M, Xiao X. Heat shock pretreatment inhibited the release of Smac/DIABLO from mitochondria and apoptosis induced by hydrogen peroxide in cardiomyocytes and C2C12 myogenic cells. Cell Stress Chaperones. 2005;10:252-62 pubmed
Stiburek L, Vesela K, Hansikova H, Pecina P, Tesarova M, Cerna L, et al. Tissue-specific cytochrome c oxidase assembly defects due to mutations in SCO2 and SURF1. Biochem J. 2005;392:625-32 pubmed
Guo Y, Cheong N, Zhang Z, De Rose R, Deng Y, Farber S, et al. Tim50, a component of the mitochondrial translocator, regulates mitochondrial integrity and cell death. J Biol Chem. 2004;279:24813-25 pubmed
Chandra D, Tang D. Mitochondrially localized active caspase-9 and caspase-3 result mostly from translocation from the cytosol and partly from caspase-mediated activation in the organelle. Lack of evidence for Apaf-1-mediated procaspase-9 activation in the mitochondria. J Biol Chem. 2003;278:17408-20 pubmed
Chandra D, Liu J, Tang D. Early mitochondrial activation and cytochrome c up-regulation during apoptosis. J Biol Chem. 2002;277:50842-54 pubmed
Guo Y, Srinivasula S, Druilhe A, Fernandes Alnemri T, Alnemri E. Caspase-2 induces apoptosis by releasing proapoptotic proteins from mitochondria. J Biol Chem. 2002;277:13430-7 pubmed
Mosser D, Caron A, Bourget L, Meriin A, Sherman M, Morimoto R, et al. The chaperone function of hsp70 is required for protection against stress-induced apoptosis. Mol Cell Biol. 2000;20:7146-59 pubmed
Syken J, De Medina T, Munger K. TID1, a human homolog of the Drosophila tumor suppressor l(2)tid, encodes two mitochondrial modulators of apoptosis with opposing functions. Proc Natl Acad Sci U S A. 1999;96:8499-504 pubmed
product information
Product Type :
Antibody
Product Name :
MTCO2 Monoclonal Antibody (12C4F12)
Catalog # :
A-6404
Quantity :
100 µg
Price :
US 399.00
Clonality :
Monoclonal
Purity :
purified
Host :
Mouse
Reactivity :
Human
Applications :
Immunocytochemistry: 5-10 µg/mL, Western Blot: 1 µg/mL
Species :
Human
Clone :
12C4F12
Isotype :
IgG2a, kappa
Storage :
4° C, do not freeze
Description :
Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix. [UniProt]
Immunogen :
Full-length native Human Cytochrome C oxidase (Complex IV) subunit II, from SDS-PAGE
Format :
Liquid
Applications w/Dilutions :
Immunocytochemistry: 5-10 µg/mL, Western Blot: 1 µg/mL
Aliases :
ACI60_gp02; Co2; COII; COX2; COXII; cyclooxygenase; cytchrome c oxidase sububnit 2; Cytochrome c oxidase polypeptide II; Cytochrome c oxidase subunit 2; cytochrome c oxidase subunit II; mitochondrially encoded cytochrome c oxidase II; Mtco2; MT-CO2; OXI1; OXII; Q0250
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA