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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
ATP Synthase beta Monoclonal Antibody (3D5AB1)
catalog :
A-21351
quantity :
100 µg
price :
US 339.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
3D5AB1
reactivity :
African green monkey, human, mouse, rat, bovine, fruit fly , roundworm
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 73
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; human; loading ...; fig 3a
Murakawa T, Okamoto K, Omiya S, Taneike M, Yamaguchi O, Otsu K. A Mammalian Mitophagy Receptor, Bcl2-L-13, Recruits the ULK1 Complex to Induce Mitophagy. Cell Rep. 2019;26:338-345.e6 pubmed publisher
  • western blot; human; 1:2000; loading ...; fig 1b
Wanet A, Caruso M, Domelevo Entfellner J, Najar M, Fattaccioli A, Demazy C, et al. The Transcription Factor 7-Like 2-Peroxisome Proliferator-Activated Receptor Gamma Coactivator-1 Alpha Axis Connects Mitochondrial Biogenesis and Metabolic Shift with Stem Cell Commitment to Hepatic Differentiation. Stem Cells. 2017;35:2184-2197 pubmed publisher
  • western blot; rat; loading ...; fig 2a
Hardonnière K, Fernier M, Gallais I, Mograbi B, Podechard N, Le Ferrec E, et al. Role for the ATPase inhibitory factor 1 in the environmental carcinogen-induced Warburg phenotype. Sci Rep. 2017;7:195 pubmed publisher
  • immunocytochemistry; rat; 1:500; loading ...
Moulis M, Millet A, Daloyau M, Miquel M, Ronsin B, Wissinger B, et al. OPA1 haploinsufficiency induces a BNIP3-dependent decrease in mitophagy in neurons: relevance to Dominant Optic Atrophy. J Neurochem. 2017;140:485-494 pubmed publisher
  • western blot; human; loading ...
Cenini G, Rüb C, Bruderek M, Voos W. Amyloid ?-peptides interfere with mitochondrial preprotein import competence by a coaggregation process. Mol Biol Cell. 2016;27:3257-3272 pubmed
  • western blot; human
Fujikawa M, Sugawara K, Tanabe T, Yoshida M. Assembly of human mitochondrial ATP synthase through two separate intermediates, F1-c-ring and b-e-g complex. FEBS Lett. 2015;589:2707-12 pubmed publisher
  • western blot; human; 1:1000
Scifo E, Szwajda A, Soliymani R, Pezzini F, Bianchi M, Dapkunas A, et al. Quantitative analysis of PPT1 interactome in human neuroblastoma cells. Data Brief. 2015;4:207-16 pubmed publisher
  • immunohistochemistry; mouse; 1:100
Gonzalez S, Fernando R, Berthelot J, Perrin Tricaud C, Sarzi E, Chrast R, et al. In vivo time-lapse imaging of mitochondria in healthy and diseased peripheral myelin sheath. Mitochondrion. 2015;23:32-41 pubmed publisher
  • western blot; human; 1:1000
Scifo E, Szwajda A, Soliymani R, Pezzini F, Bianchi M, Dapkunas A, et al. Proteomic analysis of the palmitoyl protein thioesterase 1 interactome in SH-SY5Y human neuroblastoma cells. J Proteomics. 2015;123:42-53 pubmed publisher
  • flow cytometry; human
  • immunocytochemistry; human
González Pecchi V, Valdés S, Pons V, Honorato P, Martinez L, Lamperti L, et al. Apolipoprotein A-I enhances proliferation of human endothelial progenitor cells and promotes angiogenesis through the cell surface ATP synthase. Microvasc Res. 2015;98:9-15 pubmed publisher
  • western blot; fruit fly ; fig 3
Fongsaran C, Jirakanwisal K, Kuadkitkan A, Wikan N, Wintachai P, Thepparit C, et al. Involvement of ATP synthase β subunit in chikungunya virus entry into insect cells. Arch Virol. 2014;159:3353-64 pubmed publisher
  • western blot; mouse
White A, Philp A, Fridolfsson H, Schilling J, Murphy A, Hamilton D, et al. High-fat diet-induced impairment of skeletal muscle insulin sensitivity is not prevented by SIRT1 overexpression. Am J Physiol Endocrinol Metab. 2014;307:E764-72 pubmed publisher
  • western blot; fruit fly ; 1:1500; fig 5s
Burman J, Itsara L, Kayser E, Suthammarak W, Wang A, Kaeberlein M, et al. A Drosophila model of mitochondrial disease caused by a complex I mutation that uncouples proton pumping from electron transfer. Dis Model Mech. 2014;7:1165-74 pubmed publisher
  • western blot; fruit fly ; 1:2000
Thomas R, Andrews L, Burman J, Lin W, Pallanck L. PINK1-Parkin pathway activity is regulated by degradation of PINK1 in the mitochondrial matrix. PLoS Genet. 2014;10:e1004279 pubmed publisher
  • immunocytochemistry; human; fig 1, 5
Fujikawa M, Ohsakaya S, Sugawara K, Yoshida M. Population of ATP synthase molecules in mitochondria is limited by available 6.8-kDa proteolipid protein (MLQ). Genes Cells. 2014;19:153-60 pubmed publisher
  • western blot; mouse; fig 3
Nakamura J, Fujikawa M, Yoshida M. IF1, a natural inhibitor of mitochondrial ATP synthase, is not essential for the normal growth and breeding of mice. Biosci Rep. 2013;33: pubmed publisher
  • western blot; mouse
Valente A, Yoshida T, Clark R, Delafontaine P, Siebenlist U, Chandrasekar B. Advanced oxidation protein products induce cardiomyocyte death via Nox2/Rac1/superoxide-dependent TRAF3IP2/JNK signaling. Free Radic Biol Med. 2013;60:125-35 pubmed publisher
  • western blot; human; 1:10000
McNally M, Soane L, Roelofs B, Hartman A, Hardwick J. The N-terminal helix of Bcl-xL targets mitochondria. Mitochondrion. 2013;13:119-24 pubmed publisher
  • western blot; mouse; fig 5
Guo W, Liu S, Peng J, Wei X, Sun Y, Qiu Y, et al. Examining the interactome of huperzine A by magnetic biopanning. PLoS ONE. 2012;7:e37098 pubmed publisher
  • western blot; human; 1:4000; fig 2
Deshpande M, Notari L, Subramanian P, Notario V, Becerra S. Inhibition of tumor cell surface ATP synthesis by pigment epithelium-derived factor: implications for antitumor activity. Int J Oncol. 2012;41:219-27 pubmed publisher
  • western blot; human; fig 1
Fujikawa M, Imamura H, Nakamura J, Yoshida M. Assessing actual contribution of IF1, inhibitor of mitochondrial FoF1, to ATP homeostasis, cell growth, mitochondrial morphology, and cell viability. J Biol Chem. 2012;287:18781-7 pubmed publisher
  • western blot; fruit fly ; fig 7
Celotto A, Chiu W, Van Voorhies W, Palladino M. Modes of metabolic compensation during mitochondrial disease using the Drosophila model of ATP6 dysfunction. PLoS ONE. 2011;6:e25823 pubmed publisher
  • western blot; roundworm ; fig 2
Fujii M, Yasuda K, Hartman P, Ayusawa D, Ishii N. A mutation in a mitochondrial dehydrogenase/reductase gene causes an increased sensitivity to oxidative stress and mitochondrial defects in the nematode Caenorhabditis elegans. Genes Cells. 2011;16:1022-34 pubmed publisher
  • western blot; human; 1:2500; fig 6
Pircher H, Straganz G, Ehehalt D, Morrow G, Tanguay R, Jansen Durr P. Identification of human fumarylacetoacetate hydrolase domain-containing protein 1 (FAHD1) as a novel mitochondrial acylpyruvase. J Biol Chem. 2011;286:36500-8 pubmed publisher
  • western blot; mouse; fig 3
Philp A, Belew M, Evans A, Pham D, Sivia I, Chen A, et al. The PGC-1?-related coactivator promotes mitochondrial and myogenic adaptations in C2C12 myotubes. Am J Physiol Regul Integr Comp Physiol. 2011;301:R864-72 pubmed publisher
  • western blot; mouse; fig s1
Donohoe D, Garge N, Zhang X, Sun W, O Connell T, Bunger M, et al. The microbiome and butyrate regulate energy metabolism and autophagy in the mammalian colon. Cell Metab. 2011;13:517-26 pubmed publisher
  • western blot; human
Ohsakaya S, Fujikawa M, Hisabori T, Yoshida M. Knockdown of DAPIT (diabetes-associated protein in insulin-sensitive tissue) results in loss of ATP synthase in mitochondria. J Biol Chem. 2011;286:20292-6 pubmed publisher
  • western blot; human; fig 8
Hackenbeck T, Huber R, Schietke R, Knaup K, Monti J, Wu X, et al. The GTPase RAB20 is a HIF target with mitochondrial localization mediating apoptosis in hypoxia. Biochim Biophys Acta. 2011;1813:1-13 pubmed publisher
  • western blot; human; 1:3000; fig 3
Cuadrado E, Rosell A, Colome N, Hernandez Guillamon M, García Berrocoso T, Ribo M, et al. The proteome of human brain after ischemic stroke. J Neuropathol Exp Neurol. 2010;69:1105-15 pubmed publisher
  • western blot; human; fig 4
Fujikawa M, Yoshida M. A sensitive, simple assay of mitochondrial ATP synthesis of cultured mammalian cells suitable for high-throughput analysis. Biochem Biophys Res Commun. 2010;401:538-43 pubmed publisher
  • western blot; mouse; fig 1
Philp A, Perez Schindler J, Green C, Hamilton D, Baar K. Pyruvate suppresses PGC1alpha expression and substrate utilization despite increased respiratory chain content in C2C12 myotubes. Am J Physiol Cell Physiol. 2010;299:C240-50 pubmed publisher
  • western blot; mouse; fig 3
Venkatesan B, Prabhu S, Venkatachalam K, Mummidi S, Valente A, Clark R, et al. WNT1-inducible signaling pathway protein-1 activates diverse cell survival pathways and blocks doxorubicin-induced cardiomyocyte death. Cell Signal. 2010;22:809-20 pubmed publisher
  • immunohistochemistry - frozen section; human; 2 ug/ml; fig 19.2.1
  • immunohistochemistry - paraffin section; human; 2 ug/ml; fig 19.2.2
  • immunocytochemistry; human; 2 ug/ml; fig 19.2.3
De Paepe B, De Bleecker J, Van Coster R. Histochemical methods for the diagnosis of mitochondrial diseases. Curr Protoc Hum Genet. 2009;Chapter 19:Unit19.2 pubmed publisher
  • immunohistochemistry - paraffin section; rat; 1:200
Ogawa K, Harada K, Endo Y, Sagawa S, Inoue M. Heterogeneous levels of oxidative phosphorylation enzymes in rat adrenal glands. Acta Histochem. 2011;113:24-31 pubmed publisher
  • western blot; human; fig 3
Blatt N, Boitano A, Lyssiotis C, Opipari A, Glick G. Bz-423 superoxide signals B cell apoptosis via Mcl-1, Bak, and Bax. Biochem Pharmacol. 2009;78:966-73 pubmed publisher
  • immunocytochemistry; African green monkey; 0.5-4 ug/ml; fig 2
van de Linde S, Sauer M, Heilemann M. Subdiffraction-resolution fluorescence imaging of proteins in the mitochondrial inner membrane with photoswitchable fluorophores. J Struct Biol. 2008;164:250-4 pubmed publisher
  • western blot; mouse
Blatt N, Boitano A, Lyssiotis C, Opipari A, Glick G. Bz-423 superoxide signals apoptosis via selective activation of JNK, Bak, and Bax. Free Radic Biol Med. 2008;45:1232-42 pubmed publisher
  • western blot; human; 1:5000; fig 5
Nordgaard C, Karunadharma P, Feng X, Olsen T, Ferrington D. Mitochondrial proteomics of the retinal pigment epithelium at progressive stages of age-related macular degeneration. Invest Ophthalmol Vis Sci. 2008;49:2848-55 pubmed publisher
  • immunocytochemistry; rat; fig 10
Staudt T, Lang M, Medda R, Engelhardt J, Hell S. 2,2'-thiodiethanol: a new water soluble mounting medium for high resolution optical microscopy. Microsc Res Tech. 2007;70:1-9 pubmed
  • western blot; mouse
Martin L, Liu Z, Chen K, Price A, Pan Y, Swaby J, et al. Motor neuron degeneration in amyotrophic lateral sclerosis mutant superoxide dismutase-1 transgenic mice: mechanisms of mitochondriopathy and cell death. J Comp Neurol. 2007;500:20-46 pubmed
  • western blot; human
Mazzanti R, Giulivi C. Coordination of nuclear- and mitochondrial-DNA encoded proteins in cancer and normal colon tissues. Biochim Biophys Acta. 2006;1757:618-23 pubmed
  • western blot; human; fig 2
Mazzanti R, Solazzo M, Fantappiè O, Elfering S, Pantaleo P, Bechi P, et al. Differential expression proteomics of human colon cancer. Am J Physiol Gastrointest Liver Physiol. 2006;290:G1329-38 pubmed
Pareek G, Pallanck L. Inactivation of the mitochondrial protease Afg3l2 results in severely diminished respiratory chain activity and widespread defects in mitochondrial gene expression. PLoS Genet. 2020;16:e1009118 pubmed publisher
Delavallée L, Mathiah N, Cabon L, Mazeraud A, Brunelle Navas M, Lerner L, et al. Mitochondrial AIF loss causes metabolic reprogramming, caspase-independent cell death blockade, embryonic lethality, and perinatal hydrocephalus. Mol Metab. 2020;40:101027 pubmed publisher
Barodia S, McMeekin L, Creed R, Quinones E, Cowell R, Goldberg M. PINK1 phosphorylates ubiquitin predominantly in astrocytes. NPJ Parkinsons Dis. 2019;5:29 pubmed publisher
Crochemore C, Fernandez Molina C, Montagne B, Salles A, Ricchetti M. CSB promoter downregulation via histone H3 hypoacetylation is an early determinant of replicative senescence. Nat Commun. 2019;10:5576 pubmed publisher
Moschinger M, Hilse K, Rupprecht A, Zeitz U, Erben R, Rülicke T, et al. Age-related sex differences in the expression of important disease-linked mitochondrial proteins in mice. Biol Sex Differ. 2019;10:56 pubmed publisher
Fung T, Ji W, Higgs H, Chakrabarti R. Two distinct actin filament populations have effects on mitochondria, with differences in stimuli and assembly factors. J Cell Sci. 2019;132: pubmed publisher
Miggitsch C, Meryk A, Naismith E, Pangrazzi L, Ejaz A, Jenewein B, et al. Human bone marrow adipocytes display distinct immune regulatory properties. EBioMedicine. 2019;46:387-398 pubmed publisher
Tanaka H, Okazaki T, Aoyama S, Yokota M, Koike M, Okada Y, et al. Peroxisomes control mitochondrial dynamics and the mitochondrion-dependent apoptosis pathway. J Cell Sci. 2019;132: pubmed publisher
Abetov D, Kiyan V, Zhylkibayev A, Sarbassova D, Alybayev S, Spooner E, et al. Formation of mammalian preribosomes proceeds from intermediate to composed state during ribosome maturation. J Biol Chem. 2019;294:10746-10757 pubmed publisher
Tran L, Langlais P, Hoffman N, Roust L, Katsanos C. Mitochondrial ATP synthase β-subunit production rate and ATP synthase specific activity are reduced in skeletal muscle of humans with obesity. Exp Physiol. 2019;104:126-135 pubmed publisher
Pareek G, Thomas R, Vincow E, Morris D, Pallanck L. Lon protease inactivation in Drosophila causes unfolded protein stress and inhibition of mitochondrial translation. Cell Death Discov. 2018;4:51 pubmed publisher
Pan J, Tang F, Xiong F, Xiong L, Zeng P, Wang B, et al. APP promotes osteoblast survival and bone formation by regulating mitochondrial function and preventing oxidative stress. Cell Death Dis. 2018;9:1077 pubmed publisher
Palomo G, Granatiero V, Kawamata H, Konràd C, Kim M, Arreguin A, et al. Parkin is a disease modifier in the mutant SOD1 mouse model of ALS. EMBO Mol Med. 2018;10: pubmed publisher
Pareek G, Thomas R, Pallanck L. Loss of the Drosophila m-AAA mitochondrial protease paraplegin results in mitochondrial dysfunction, shortened lifespan, and neuronal and muscular degeneration. Cell Death Dis. 2018;9:304 pubmed publisher
Marie M, Bigot K, Angebault C, Barrau C, Gondouin P, Pagan D, et al. Light action spectrum on oxidative stress and mitochondrial damage in A2E-loaded retinal pigment epithelium cells. Cell Death Dis. 2018;9:287 pubmed publisher
Syu Y, Lai H, Jiang C, Tsai H, Lin C, Lee Y. GLUT10 maintains the integrity of major arteries through regulation of redox homeostasis and mitochondrial function. Hum Mol Genet. 2018;27:307-321 pubmed publisher
El Merhie N, Baumgart Vogt E, Pilatz A, Pfreimer S, Pfeiffer B, Pak O, et al. Differential Alterations of the Mitochondrial Morphology and Respiratory Chain Complexes during Postnatal Development of the Mouse Lung. Oxid Med Cell Longev. 2017;2017:9169146 pubmed publisher
Ji W, Chakrabarti R, Fan X, Schoenfeld L, Strack S, Higgs H. Receptor-mediated Drp1 oligomerization on endoplasmic reticulum. J Cell Biol. 2017;216:4123-4139 pubmed publisher
Rogers R, Tungtur S, Tanaka T, Nadeau L, Badawi Y, Wang H, et al. Impaired Mitophagy Plays a Role in Denervation of Neuromuscular Junctions in ALS Mice. Front Neurosci. 2017;11:473 pubmed publisher
Lee H, Smith S, Yoon Y. The short variant of the mitochondrial dynamin OPA1 maintains mitochondrial energetics and cristae structure. J Biol Chem. 2017;292:7115-7130 pubmed publisher
Milasta S, Dillon C, Sturm O, Verbist K, Brewer T, Quarato G, et al. Apoptosis-Inducing-Factor-Dependent Mitochondrial Function Is Required for T Cell but Not B Cell Function. Immunity. 2016;44:88-102 pubmed publisher
Wang T, McDonald C, Petrenko N, Leblanc M, Wang T, Giguere V, et al. Estrogen-related receptor α (ERRα) and ERRγ are essential coordinators of cardiac metabolism and function. Mol Cell Biol. 2015;35:1281-98 pubmed publisher
Gagnon K, Li L, Janowski B, Corey D. Analysis of nuclear RNA interference in human cells by subcellular fractionation and Argonaute loading. Nat Protoc. 2014;9:2045-60 pubmed publisher
Lechauve C, Augustin S, Cwerman Thibault H, Reboussin E, Roussel D, Lai Kuen R, et al. Neuroglobin gene therapy prevents optic atrophy and preserves durably visual function in Harlequin mice. Mol Ther. 2014;22:1096-1109 pubmed publisher
Shi Y, Pulliam D, Liu Y, Hamilton R, Jernigan A, Bhattacharya A, et al. Reduced mitochondrial ROS, enhanced antioxidant defense, and distinct age-related changes in oxidative damage in muscles of long-lived Peromyscus leucopus. Am J Physiol Regul Integr Comp Physiol. 2013;304:R343-55 pubmed publisher
De Pauw A, Demine S, Tejerina S, Dieu M, Delaive E, Kel A, et al. Mild mitochondrial uncoupling does not affect mitochondrial biogenesis but downregulates pyruvate carboxylase in adipocytes: role for triglyceride content reduction. Am J Physiol Endocrinol Metab. 2012;302:E1123-41 pubmed publisher
Chen Y, Aon M, Hsu Y, Soane L, Teng X, McCaffery J, et al. Bcl-xL regulates mitochondrial energetics by stabilizing the inner membrane potential. J Cell Biol. 2011;195:263-76 pubmed publisher
Sorgeloos F, Vertommen D, Rider M, Michiels T. Theiler's virus L* protein is targeted to the mitochondrial outer membrane. J Virol. 2011;85:3690-4 pubmed publisher
Poole A, Thomas R, Yu S, Vincow E, Pallanck L. The mitochondrial fusion-promoting factor mitofusin is a substrate of the PINK1/parkin pathway. PLoS ONE. 2010;5:e10054 pubmed publisher
Chen Y, Kao C, Chen Y, Wang C, Wu C, Tsai C, et al. Cisd2 deficiency drives premature aging and causes mitochondria-mediated defects in mice. Genes Dev. 2009;23:1183-94 pubmed publisher
Zhang X, Gao F, Yu L, Peng Y, Liu H, Liu J, et al. Dual functions of a monoclonal antibody against cell surface F1F0 ATP synthase on both HUVEC and tumor cells. Acta Pharmacol Sin. 2008;29:942-50 pubmed publisher
product information
Product Type :
Antibody
Product Name :
ATP Synthase beta Monoclonal Antibody (3D5AB1)
Catalog # :
A-21351
Quantity :
100 µg
Price :
US 339.00
Clonality :
Monoclonal
Purity :
purified
Host :
Mouse
Reactivity :
Bovine, C. elegans, Human, Mouse, Non-human primate, Rat
Applications :
Immunocytochemistry: 1-2 µg/mL, Western Blot: 0.5 µg/mL
Species :
Bovine, C. elegans, Human, Mouse, Non-human primate, Rat
Clone :
3D5AB1
Isotype :
IgG1, kappa
Storage :
4° C
Description :
ATP synthase is extremely conserved through evolution and can be found in plants, fungi, bacteria, and animals. The ATP synthase enzyme is a transmembrane protein responsible for driving the reversible reaction from ADP+ phosphate to ATP. This reaction is accomplished by a flux of protons across the membrane as a result of electron transfer. The ATP synthase protein has two main sections; the F1 ATP-ase (soluble) and the F0 ATP-ase (membrane embedded). The F1 section consists of the alpha, beta, gamma, delta, and epsilon subunits. While the F0 consists of a, b, and c subunits.
Immunogen :
Human Heart Mitochondria.
Format :
Liquid
Applications w/Dilutions :
Immunocytochemistry: 1-2 µg/mL, Western Blot: 0.5 µg/mL
Aliases :
ATP synthase F1 subunit beta; ATP synthase subunit beta, mitochondrial; ATP synthase, H+ transporting mitochondrial F1 complex, alpha subunit; ATP synthase, H+ transporting mitochondrial F1 complex, beta subunit; ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide; Atp5b; ATP5F1B; ATPMB; ATPSB; beta-subunit; epididymis secretory protein Li 271; f1-ATPase beta; F1-ATPase beta-subunit; F-1-ATPase beta-subunit precursor; fj13e04; fj55c09; HEL-S-271; hm:zehn0534; hypothetical protein LOC554135; im:6793121; MGC5231; mitochondrial ATP synthase beta subunit; mitochondrial ATP synthase subunit beta subunit; mitochondrial ATP synthase, H+ transporting F1 complex beta subunit; mitochondrial ATP synthetase, beta subunit; wu:fj13e04; wu:fj38d01; wu:fj55c09; zgc:111961
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA