product summary
Loading...
company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
OxPhos Complex IV subunit IV Monoclonal Antibody (10G8D12C12)
catalog :
A-21347
quantity :
100 µg
price :
US 339.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
10G8D12C12
reactivity :
hamsters, human, mouse, rat, bovine, rhesus macaque
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 49
Published Application/Species/Sample/DilutionReference
  • western blot; human; loading ...; fig 3b
Moren C, Gonzalez Casacuberta I, Alvarez Fernández C, Bano M, Catalán García M, Guitart Mampel M, et al. HIV-1 promonocytic and lymphoid cell lines: an in vitro model of in vivo mitochondrial and apoptotic lesion. J Cell Mol Med. 2017;21:402-409 pubmed publisher
  • western blot; human; 1:5000; loading ...; fig 2b
Reed G, Schiller G, Kambhampati S, Tallman M, Douer D, Minden M, et al. A Phase 1 study of intravenous infusions of tigecycline in patients with acute myeloid leukemia. Cancer Med. 2016;5:3031-3040 pubmed publisher
  • western blot; human; 1:500; loading ...; fig 5c
Wei R, Lin S, Wu W, Chen L, Li C, Chen H, et al. A microtubule inhibitor, ABT-751, induces autophagy and delays apoptosis in Huh-7 cells. Toxicol Appl Pharmacol. 2016;311:88-98 pubmed publisher
  • western blot; human; loading ...; fig 3c
Maughan R, Feeney E, Capel E, Capeau J, Domingo P, Giralt M, et al. Improved adipose tissue function with initiation of protease inhibitor-only ART. J Antimicrob Chemother. 2016;71:3212-3221 pubmed
  • western blot; human; fig 5
Scott A, Wilkinson A, Wilkinson J. Basal metabolic state governs AIF-dependent growth support in pancreatic cancer cells. BMC Cancer. 2016;16:286 pubmed publisher
  • western blot; human; fig 4
Xie W, Zhang L, Jiao H, Guan L, Zha J, Li X, et al. Chaperone-mediated autophagy prevents apoptosis by degrading BBC3/PUMA. Autophagy. 2015;11:1623-35 pubmed publisher
  • western blot; human
Kim T, Kim J, Kim Z, Huang R, Chae Y, Wang R. Khz-cp (crude polysaccharide extract obtained from the fusion of Ganoderma lucidum and Polyporus umbellatus mycelia) induces apoptosis by increasing intracellular calcium levels and activating P38 and NADPH oxidase-dependent generation of reactive ox. BMC Complement Altern Med. 2014;14:236 pubmed publisher
  • western blot; rat; fig 6
Smith B, Perry C, Herbst E, Ritchie I, Beaudoin M, Smith J, et al. Submaximal ADP-stimulated respiration is impaired in ZDF rats and recovered by resveratrol. J Physiol. 2013;591:6089-101 pubmed publisher
  • western blot; human; fig 5
Lewis E, Wilkinson A, Jackson J, Mehra R, Varambally S, Chinnaiyan A, et al. The enzymatic activity of apoptosis-inducing factor supports energy metabolism benefiting the growth and invasiveness of advanced prostate cancer cells. J Biol Chem. 2012;287:43862-75 pubmed publisher
  • western blot; human; 1:2000; fig 7
Kuo K, Chen Y, Chen L, Li C, Lan Y, Chang F, et al. Involvement of phorbol-12-myristate-13-acetate-induced protein 1 in goniothalamin-induced TP53-dependent and -independent apoptosis in hepatocellular carcinoma-derived cells. Toxicol Appl Pharmacol. 2011;256:8-23 pubmed publisher
  • western blot; mouse; fig 2
Kim J, Yu S, Oh H, Lee J, Kim Y, Sohn J. Panaxydol induces apoptosis through an increased intracellular calcium level, activation of JNK and p38 MAPK and NADPH oxidase-dependent generation of reactive oxygen species. Apoptosis. 2011;16:347-58 pubmed publisher
  • western blot; human; fig 4
Hou W, Han J, Lu C, Goldstein L, Rabinowich H. Autophagic degradation of active caspase-8: a crosstalk mechanism between autophagy and apoptosis. Autophagy. 2010;6:891-900 pubmed publisher
  • western blot; rat; fig 1
McConell G, Ng G, Phillips M, Ruan Z, Macaulay S, Wadley G. Central role of nitric oxide synthase in AICAR and caffeine-induced mitochondrial biogenesis in L6 myocytes. J Appl Physiol (1985). 2010;108:589-95 pubmed publisher
  • western blot; human; fig 3
Garibal J, Hollville E, Renouf B, Tétaud C, Wiels J. Caspase-8-mediated cleavage of Bid and protein phosphatase 2A-mediated activation of Bax are necessary for Verotoxin-1-induced apoptosis in Burkitt's lymphoma cells. Cell Signal. 2010;22:467-75 pubmed publisher
  • western blot; human; fig 1
Kutuk O, Arisan E, Tezil T, Shoshan M, Basaga H. Cisplatin overcomes Bcl-2-mediated resistance to apoptosis via preferential engagement of Bak: critical role of Noxa-mediated lipid peroxidation. Carcinogenesis. 2009;30:1517-27 pubmed publisher
  • western blot; human
Yee K, Wilkinson S, James J, Ryan K, Vousden K. PUMA- and Bax-induced autophagy contributes to apoptosis. Cell Death Differ. 2009;16:1135-45 pubmed publisher
  • western blot; human; fig 1
Trougakos I, Lourda M, Antonelou M, Kletsas D, Gorgoulis V, Papassideri I, et al. Intracellular clusterin inhibits mitochondrial apoptosis by suppressing p53-activating stress signals and stabilizing the cytosolic Ku70-Bax protein complex. Clin Cancer Res. 2009;15:48-59 pubmed publisher
  • western blot; human; fig 6
Sibayama Imazu T, Fujisawa Y, Masuda Y, Aiuchi T, Nakajo S, Itabe H, et al. Induction of apoptosis in PA-1 ovarian cancer cells by vitamin K2 is associated with an increase in the level of TR3/Nur77 and its accumulation in mitochondria and nuclei. J Cancer Res Clin Oncol. 2008;134:803-12 pubmed publisher
  • western blot; rat; 1:1000; fig 2
Castello P, Drechsel D, Day B, Patel M. Inhibition of mitochondrial hydrogen peroxide production by lipophilic metalloporphyrins. J Pharmacol Exp Ther. 2008;324:970-6 pubmed
  • western blot; human; fig 4c
Yee K, Vousden K. Contribution of membrane localization to the apoptotic activity of PUMA. Apoptosis. 2008;13:87-95 pubmed
  • western blot; human; fig 3
Autret A, Martin Latil S, Mousson L, Wirotius A, Petit F, Arnoult D, et al. Poliovirus induces Bax-dependent cell death mediated by c-Jun NH2-terminal kinase. J Virol. 2007;81:7504-16 pubmed
  • western blot; rat; 1:1000
Castello P, Drechsel D, Patel M. Mitochondria are a major source of paraquat-induced reactive oxygen species production in the brain. J Biol Chem. 2007;282:14186-93 pubmed
  • western blot; rhesus macaque
Martin Latil S, Mousson L, Autret A, Colbere Garapin F, Blondel B. Bax is activated during rotavirus-induced apoptosis through the mitochondrial pathway. J Virol. 2007;81:4457-64 pubmed
  • western blot; rat; 1:20,000
Brown M, Sullivan P, Geddes J. Synaptic mitochondria are more susceptible to Ca2+overload than nonsynaptic mitochondria. J Biol Chem. 2006;281:11658-68 pubmed
  • western blot; human; fig 1
Iuso A, Scacco S, Piccoli C, Bellomo F, Petruzzella V, Trentadue R, et al. Dysfunctions of cellular oxidative metabolism in patients with mutations in the NDUFS1 and NDUFS4 genes of complex I. J Biol Chem. 2006;281:10374-80 pubmed
  • western blot; rat; 1:1000; fig 6A
Ishihara Y, Shimamoto N. Involvement of endonuclease G in nucleosomal DNA fragmentation under sustained endogenous oxidative stress. J Biol Chem. 2006;281:6726-33 pubmed
  • western blot; human; fig 4
Boehning D, van Rossum D, Patterson R, Snyder S. A peptide inhibitor of cytochrome c/inositol 1,4,5-trisphosphate receptor binding blocks intrinsic and extrinsic cell death pathways. Proc Natl Acad Sci U S A. 2005;102:1466-71 pubmed
  • immunohistochemistry - paraffin section; mouse; 1:100; fig 2
Plesnila N, Zhu C, Culmsee C, Gröger M, Moskowitz M, Blomgren K. Nuclear translocation of apoptosis-inducing factor after focal cerebral ischemia. J Cereb Blood Flow Metab. 2004;24:458-66 pubmed
  • western blot; mouse; fig 1b
Lustbader J, Cirilli M, Lin C, Xu H, Takuma K, Wang N, et al. ABAD directly links Abeta to mitochondrial toxicity in Alzheimer's disease. Science. 2004;304:448-52 pubmed
  • immunocytochemistry; human; fig 4
Thomenius M, Wang N, Reineks E, Wang Z, Distelhorst C. Bcl-2 on the endoplasmic reticulum regulates Bax activity by binding to BH3-only proteins. J Biol Chem. 2003;278:6243-50 pubmed
  • immunohistochemistry - paraffin section; rat; 1:200; fig 1
Walzel B, Speer O, Zanolla E, Eriksson O, Bernardi P, Wallimann T. Novel mitochondrial creatine transport activity. Implications for intracellular creatine compartments and bioenergetics. J Biol Chem. 2002;277:37503-11 pubmed
  • immunocytochemistry; hamsters; fig 2
Pan Z, Bhat M, Nieminen A, Ma J. Synergistic movements of Ca(2+) and Bax in cells undergoing apoptosis. J Biol Chem. 2001;276:32257-63 pubmed
  • immunohistochemistry - frozen section; human; 1:100; fig 2
Tanji K, Bonilla E. Optical imaging techniques (histochemical, immunohistochemical, and in situ hybridization staining methods) to visualize mitochondria. Methods Cell Biol. 2001;65:311-32 pubmed
  • western blot; human
Griffiths G, Dubrez L, Morgan C, Jones N, Whitehouse J, Corfe B, et al. Cell damage-induced conformational changes of the pro-apoptotic protein Bak in vivo precede the onset of apoptosis. J Cell Biol. 1999;144:903-14 pubmed
  • western blot; human; 1 ug/ml; fig 4
Marusich M, Robinson B, Taanman J, Kim S, Schillace R, Smith J, et al. Expression of mtDNA and nDNA encoded respiratory chain proteins in chemically and genetically-derived Rho0 human fibroblasts: a comparison of subunit proteins in normal fibroblasts treated with ethidium bromide and fibroblasts from a patient with mtD. Biochim Biophys Acta. 1997;1362:145-59 pubmed
  • western blot; human; fig 1
Taanman J, Burton M, Marusich M, Kennaway N, Capaldi R. Subunit specific monoclonal antibodies show different steady-state levels of various cytochrome-c oxidase subunits in chronic progressive external ophthalmoplegia. Biochim Biophys Acta. 1996;1315:199-207 pubmed
  • western blot; human; fig 3
  • western blot; bovine; fig 3
Capaldi R, Marusich M, Taanman J. Mammalian cytochrome-c oxidase: characterization of enzyme and immunological detection of subunits in tissue extracts and whole cells. Methods Enzymol. 1995;260:117-32 pubmed
  • western blot; rat
Nangaku M, Sato Yoshitake R, Okada Y, Noda Y, Takemura R, Yamazaki H, et al. KIF1B, a novel microtubule plus end-directed monomeric motor protein for transport of mitochondria. Cell. 1994;79:1209-20 pubmed
Somuncu B, Ekmekcioglu A, Antmen F, Ertuzun T, Deniz E, Keskin N, et al. Targeting mitochondrial DNA polymerase gamma for selective inhibition of MLH1 deficient colon cancer growth. PLoS ONE. 2022;17:e0268391 pubmed publisher
Inak G, Rybak Wolf A, Lisowski P, Pentimalli T, Jüttner R, Glažar P, et al. Defective metabolic programming impairs early neuronal morphogenesis in neural cultures and an organoid model of Leigh syndrome. Nat Commun. 2021;12:1929 pubmed publisher
Mirali S, Botham A, Voisin V, Xu C, St Germain J, Sharon D, et al. The mitochondrial peptidase, neurolysin, regulates respiratory chain supercomplex formation and is necessary for AML viability. Sci Transl Med. 2020;12: pubmed publisher
Pignanelli C, Petrick H, Keyvani F, Heigenhauser G, Quadrilatero J, Holloway G, et al. Low-load resistance training to task failure with and without blood flow restriction: muscular functional and structural adaptations. Am J Physiol Regul Integr Comp Physiol. 2020;318:R284-R295 pubmed publisher
Suh D, Park S, Jin H, Lee K, Bae J. LRIG2 is a growth suppressor of Hec-1A and Ishikawa endometrial adenocarcinoma cells by regulating PI3K/AKT- and EGFR-mediated apoptosis and cell-cycle. Oncogenesis. 2018;7:3 pubmed publisher
Srinivasan S, Cesa L, Li X, Julien O, Zhuang M, Shao H, et al. Heat Shock Protein 70 (Hsp70) Suppresses RIP1-Dependent Apoptotic and Necroptotic Cascades. Mol Cancer Res. 2018;16:58-68 pubmed publisher
Nag T, Wadhwa S. Immunolocalisation pattern of complex I-V in ageing human retina: Correlation with mitochondrial ultrastructure. Mitochondrion. 2016;31:20-32 pubmed publisher
Ripolone M, Ronchi D, Violano R, Vallejo D, Fagiolari G, Barca E, et al. Impaired Muscle Mitochondrial Biogenesis and Myogenesis in Spinal Muscular Atrophy. JAMA Neurol. 2015;72:666-75 pubmed publisher
Kim T, Kim J, Kim Z, Huang R, Wang R. Khz (fusion of Ganoderma lucidum and Polyporus umbellatus mycelia) induces apoptosis by increasing intracellular calcium levels and activating JNK and NADPH oxidase-dependent generation of reactive oxygen species. PLoS ONE. 2012;7:e46208 pubmed publisher
Vitte J, Traver S, Maués De Paula A, Lesage S, Rovelli G, Corti O, et al. Leucine-rich repeat kinase 2 is associated with the endoplasmic reticulum in dopaminergic neurons and accumulates in the core of Lewy bodies in Parkinson disease. J Neuropathol Exp Neurol. 2010;69:959-72 pubmed publisher
Masuda Y, Shima G, Aiuchi T, Horie M, Hori K, Nakajo S, et al. Involvement of tumor necrosis factor receptor-associated protein 1 (TRAP1) in apoptosis induced by beta-hydroxyisovalerylshikonin. J Biol Chem. 2004;279:42503-15 pubmed
product information
Product Type :
Antibody
Product Name :
OxPhos Complex IV subunit IV Monoclonal Antibody (10G8D12C12)
Catalog # :
A-21347
Quantity :
100 µg
Price :
US 339.00
Clonality :
Monoclonal
Purity :
purified
Host :
Mouse
Reactivity :
Bovine, Human
Applications :
Immunohistochemistry (Paraffin): 1:20, Western Blot: 1.0 µg/mL
Species :
Bovine, Human
Clone :
10G8D12C12
Isotype :
IgG2a, kappa
Storage :
4° C
Description :
Complex IV, also called cytochrome c oxidoreductase or cytochrome c oxidase, is a complex of 13 different subunits, three of which (I, II and III) are encoded on mitochondrial DNA and the remainder in the nuclear DNA. The complex contains two heme groups (a and a3) and two copper atoms as prosthetic groups.
Immunogen :
Bovine Complex IV subunit IV
Format :
Liquid
Applications w/Dilutions :
Immunohistochemistry (Paraffin): 1:20, Western Blot: 1.0 µg/mL
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA