product summary
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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
GFP Polyclonal Antibody, Alexa Fluor™ 488
catalog :
A-21311
quantity :
100 µL
price :
US 530
clonality :
polyclonal
host :
domestic rabbit
conjugate :
AF488
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 266
Reference
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product information
Product Type :
Antibody
Product Name :
GFP Polyclonal Antibody, Alexa Fluor™ 488
Catalog # :
A-21311
Quantity :
100 µL
Price :
US 530
Clonality :
Polyclonal
Purity :
IgG fraction
Host :
Rabbit
Reactivity :
Tag
Applications :
Immunocytochemistry: 1-10 µg/mL, Immunohistochemistry: 1:200-1:1,000, Western Blot: 1:2,000
Species :
Tag
Isotype :
IgG
Storage :
4° C, store in dark
Description :
The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights in the physiological activities of living cells. GFP is a 27 kDa monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors. GFP fluorescence is stable, species independent and is suitable for a variety of applications. GFP has been used extensively as a fluorescent tag to monitor gene expressin and protein localization. Moreover, other applications for GFP include its use in assessing protein protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments.
Immunogen :
The GFP was isolated directly from the jellyfish Aequorea victoria.
Format :
Liquid
Applications w/Dilutions :
Immunocytochemistry: 1-10 µg/mL, Immunohistochemistry: 1:200-1:1,000, Western Blot: 1:2,000
Aliases :
GFP; GFP tag; GFP2; green fluorescence; green fluorescent; Turbo eGFP
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA