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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
GFAP Monoclonal Antibody (131-17719), Alexa Fluor™ 488
catalog :
A-21294
quantity :
50 µL
price :
US 488
clonality :
monoclonal
host :
mouse
conjugate :
AF488
clone name :
131-17719
reactivity :
human, mouse
application :
immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - frozen section
more info or order :
Invitrogen product webpage
citations: 17
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - frozen section; mouse; 5 ug/ml
Steffensen M, Fenger C, Christensen J, Jørgensen C, Bassi M, Christensen J, et al. Suppressors of cytokine signaling 1 and 3 are upregulated in brain resident cells in response to virus-induced inflammation of the central nervous system via at least two distinctive pathways. J Virol. 2014;88:14090-104 pubmed publisher
  • immunocytochemistry; mouse; 1:400
Ghosh D, Levault K, Brewer G. Dual-energy precursor and nuclear erythroid-related factor 2 activator treatment additively improve redox glutathione levels and neuron survival in aging and Alzheimer mouse neurons upstream of reactive oxygen species. Neurobiol Aging. 2014;35:179-90 pubmed publisher
  • immunohistochemistry - frozen section; mouse; fig 5
Wainwright D, Balyasnikova I, Chang A, Ahmed A, Moon K, Auffinger B, et al. IDO expression in brain tumors increases the recruitment of regulatory T cells and negatively impacts survival. Clin Cancer Res. 2012;18:6110-21 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 5 ug/ml
Christensen J, Fenger C, Issazadeh Navikas S, Krug A, Liljestrøm P, Goriely S, et al. Differential impact of interferon regulatory factor 7 in initiation of the type I interferon response in the lymphocytic choriomeningitis virus-infected central nervous system versus the periphery. J Virol. 2012;86:7384-92 pubmed publisher
  • flow cytometry; mouse; fig 3
Furr S, Chauhan V, Moerdyk Schauwecker M, Marriott I. A role for DNA-dependent activator of interferon regulatory factor in the recognition of herpes simplex virus type 1 by glial cells. J Neuroinflammation. 2011;8:99 pubmed publisher
  • immunohistochemistry - frozen section; mouse; fig 1
Wainwright D, Sengupta S, Han Y, Ulasov I, Lesniak M. The presence of IL-17A and T helper 17 cells in experimental mouse brain tumors and human glioma. PLoS ONE. 2010;5:e15390 pubmed publisher
  • flow cytometry; mouse; fig 5
Chauhan V, Furr S, Sterka D, Nelson D, Moerdyk Schauwecker M, Marriott I, et al. Vesicular stomatitis virus infects resident cells of the central nervous system and induces replication-dependent inflammatory responses. Virology. 2010;400:187-96 pubmed publisher
  • flow cytometry; mouse; fig 2
Moussaud S, Draheim H. A new method to isolate microglia from adult mice and culture them for an extended period of time. J Neurosci Methods. 2010;187:243-53 pubmed publisher
  • immunohistochemistry - frozen section; mouse; fig 3
Wainwright D, Xin J, Mesnard N, Sanders V, Jones K. Toll-like receptor 2 and facial motoneuron survival after facial nerve axotomy. Neurosci Lett. 2010;471:10-4 pubmed publisher
  • immunohistochemistry - frozen section; mouse
Wainwright D, Xin J, Mesnard N, Politis C, Sanders V, Jones K. Effects of facial nerve axotomy on Th2- and Th1-associated chemokine expression in the facial motor nucleus of wild-type and presymptomatic mSOD1 mice. J Neuroimmunol. 2009;216:66-75 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 5 ug/ml; fig 10
Christensen J, Simonsen S, Fenger C, Sørensen M, Moos T, Christensen J, et al. Fulminant lymphocytic choriomeningitis virus-induced inflammation of the CNS involves a cytokine-chemokine-cytokine-chemokine cascade. J Immunol. 2009;182:1079-87 pubmed
  • immunohistochemistry - frozen section; mouse; 1:200
Tang X, Wang Q, Koike M, Cheng D, Goris M, Blankenberg F, et al. Monitoring the protective effects of minocycline treatment with radiolabeled annexin V in an experimental model of focal cerebral ischemia. J Nucl Med. 2007;48:1822-8 pubmed
Lund M, Ellman D, Nielsen P, Raffaele S, Fumagalli M, Guzman R, et al. Selective Inhibition of Soluble Tumor Necrosis Factor Alters the Neuroinflammatory Response following Moderate Spinal Cord Injury in Mice. Biology (Basel). 2023;12: pubmed publisher
Lund M, Ellman D, Nissen M, Nielsen P, Nielsen P, J xf8 rgensen C, et al. The Inflammatory Response after Moderate Contusion Spinal Cord Injury: A Time Study. Biology (Basel). 2022;11: pubmed publisher
O Sullivan S, Malahias E, Park J, Srivastava A, Reyes B, Gorky J, et al. Single-Cell Glia and Neuron Gene Expression in the Central Amygdala in Opioid Withdrawal Suggests Inflammation With Correlated Gut Dysbiosis. Front Neurosci. 2019;13:665 pubmed publisher
Griffith C, Xie M, Qiu W, Sharp A, Ma C, Pan A, et al. Aberrant expression of the pore-forming KATP channel subunit Kir6.2 in hippocampal reactive astrocytes in the 3xTg-AD mouse model and human Alzheimer's disease. Neuroscience. 2016;336:81-101 pubmed publisher
Brewer G, Boehler M, Leondopulos S, Pan L, Alagapan S, Demarse T, et al. Toward a self-wired active reconstruction of the hippocampal trisynaptic loop: DG-CA3. Front Neural Circuits. 2013;7:165 pubmed publisher
product information
Product Type :
Antibody
Product Name :
GFAP Monoclonal Antibody (131-17719), Alexa Fluor™ 488
Catalog # :
A-21294
Quantity :
50 µL
Price :
US 488
Clonality :
Monoclonal
Purity :
purified
Host :
Mouse
Reactivity :
Human
Applications :
Immunocytochemistry: 1-10 µg/mL
Species :
Human
Clone :
131-17719
Isotype :
IgG1, kappa
Storage :
4° C, store in dark
Description :
GFAP (Glial fibrillary acidic protein) is a member of the class III intermediate filament protein family. GFAP is heavily and specifically expressed in astrocytes and certain astroglia of the central nervous system, in satellite cells of peripheral ganglia, and in non-myelinating Schwann cells of peripheral nerves. In addition, neural stem cells strongly express GFAP. Antibodies to GFAP are very useful as markers of astrocytic cells. In addition, many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes. GFAP is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing of the GFAP gene results in multiple transcript variants encoding distinct isoforms.
Immunogen :
protein glial fibrillary acidic protein
Format :
Liquid
Applications w/Dilutions :
Immunocytochemistry: 1-10 µg/mL
Aliases :
6330404F12Rik; 65 kDa glutamic acid decarboxylase; AI836096; ALXDRD; Astrocyte or Intermediate Filament Protein; cb345; class III intermediate filament protein; etID36982.3; FLJ45472; GAD(65); Gad2; Gad-2; GAD65; GAD-65; GFAP; GFAP epsilon; gfap protein; gfapl; glial fibrillary acidic protein; Glial Fibrillary Acidic Protein (GFAP); glial fibrillary acidic protein alpha; glutamate decarboxylase 2; Glutamate decarboxylase 65 kDa isoform; glutamic acid decarboxylase 2; I79_011607; intermediate filament; intermediate filament protein; intermediate filament protein; GFAP; Unknown (protein for MGC:139638); wu:fb34h11; wu:fk42c12; xx:af506734; zgc:110485; zGFAP; intermediate filament protein; zrf-1 antigen
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA