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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
HuC/HuD Monoclonal Antibody (16A11), Biotin
catalog :
A-21272
quantity :
100µg
price :
US 408
clonality :
monoclonal
host :
mouse
conjugate :
biotin
clone name :
16A11
reactivity :
guinea-pigs, guinea pig, human, mouse, rat, pigs , zebrafish
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 39
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; human; 1:200; fig 1a
Brown I, Gulbransen B. The antioxidant glutathione protects against enteric neuron death in situ, but its depletion is protective during colitis. Am J Physiol Gastrointest Liver Physiol. 2018;314:G39-G52 pubmed publisher
  • immunocytochemistry; human; 1:500; fig 2
Wiley L, Burnight E, DeLuca A, Anfinson K, Cranston C, Kaalberg E, et al. cGMP production of patient-specific iPSCs and photoreceptor precursor cells to treat retinal degenerative blindness. Sci Rep. 2016;6:30742 pubmed publisher
  • immunohistochemistry; mouse; 1:200; fig 2
Bubenheimer R, Brown I, Fried D, McClain J, Gulbransen B. Sirtuin-3 Is Expressed by Enteric Neurons but It Does not Play a Major Role in Their Regulation of Oxidative Stress. Front Cell Neurosci. 2016;10:73 pubmed publisher
  • immunohistochemistry; mouse; 1:200; tbl 1
Brown I, McClain J, Watson R, Patel B, Gulbransen B. Enteric glia mediate neuron death in colitis through purinergic pathways that require connexin-43 and nitric oxide. Cell Mol Gastroenterol Hepatol. 2016;2:77-91 pubmed
  • immunohistochemistry; zebrafish ; 1:800; loading ...; fig 1a
Schill E, Lake J, Tusheva O, Nagy N, Bery S, Foster L, et al. Ibuprofen slows migration and inhibits bowel colonization by enteric nervous system precursors in zebrafish, chick and mouse. Dev Biol. 2016;409:473-88 pubmed publisher
  • immunocytochemistry; pigs ; 1:100; fig 5
Mazzuoli Weber G, Schemann M. Mechanosensitive enteric neurons in the guinea pig gastric corpus. Front Cell Neurosci. 2015;9:430 pubmed publisher
  • immunohistochemistry; mouse; 1:200; fig 1
Avetisyan M, Wang H, Schill E, Bery S, Grider J, Hassell J, et al. Hepatocyte Growth Factor and MET Support Mouse Enteric Nervous System Development, the Peristaltic Response, and Intestinal Epithelial Proliferation in Response to Injury. J Neurosci. 2015;35:11543-58 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:100; fig s13
Thys A, Vandenberghe P, Hague P, Klein O, Erneux C, Vanderwinden J. Hyperplasia of interstitial cells of cajal in sprouty homolog 4 deficient mice. PLoS ONE. 2015;10:e0124861 pubmed publisher
  • immunocytochemistry; rat; 1:600; fig 1
Voss U, Ekblad E. Lipopolysaccharide-induced loss of cultured rat myenteric neurons - role of AMP-activated protein kinase. PLoS ONE. 2014;9:e114044 pubmed publisher
  • immunohistochemistry; human
Walstab J, Wohlfarth C, Hovius R, Schmitteckert S, Roth R, Lasitschka F, et al. Natural compounds boldine and menthol are antagonists of human 5-HT3 receptors: implications for treating gastrointestinal disorders. Neurogastroenterol Motil. 2014;26:810-20 pubmed publisher
  • immunocytochemistry; mouse; 1:600
Voss U, Turesson M, Robaye B, Boeynaems J, Olde B, Erlinge D, et al. The enteric nervous system of P2Y13 receptor null mice is resistant against high-fat-diet- and palmitic-acid-induced neuronal loss. Purinergic Signal. 2014;10:455-64 pubmed publisher
  • immunohistochemistry; mouse; 1:600; fig 1
Voss U, Sand E, Olde B, Ekblad E. Enteric neuropathy can be induced by high fat diet in vivo and palmitic acid exposure in vitro. PLoS ONE. 2013;8:e81413 pubmed publisher
  • immunohistochemistry; mouse; fig 5
Lake J, Tusheva O, Graham B, Heuckeroth R. Hirschsprung-like disease is exacerbated by reduced de novo GMP synthesis. J Clin Invest. 2013;123:4875-87 pubmed
  • immunohistochemistry; mouse; 1:250; fig 1
Wright Jin E, Grider J, Duester G, Heuckeroth R. Retinaldehyde dehydrogenase enzymes regulate colon enteric nervous system structure and function. Dev Biol. 2013;381:28-37 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:200; fig 4
Shaham O, Gueta K, Mor E, Oren Giladi P, Grinberg D, Xie Q, et al. Pax6 regulates gene expression in the vertebrate lens through miR-204. PLoS Genet. 2013;9:e1003357 pubmed publisher
  • immunohistochemistry; guinea-pigs; 1:100; fig 2
Giaroni C, Marchet S, Carpanese E, Prandoni V, Oldrini R, Bartolini B, et al. Role of neuronal and inducible nitric oxide synthases in the guinea pig ileum myenteric plexus during in vitro ischemia and reperfusion. Neurogastroenterol Motil. 2013;25:e114-26 pubmed publisher
  • immunohistochemistry; human; 1:1000; fig 1
Moreira M, Brehmer A, de Oliveira E, Neto S, Luquetti A, Bueno L, et al. Regenerative process evaluation of neuronal subclasses in chagasic patients with megacolon. Hum Immunol. 2013;74:181-8 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:20; fig 7
Gross E, Gershon M, Margolis K, Gertsberg Z, Li Z, Cowles R. Neuronal serotonin regulates growth of the intestinal mucosa in mice. Gastroenterology. 2012;143:408-17.e2 pubmed publisher
  • immunohistochemistry; mouse; 1:250; fig 3
Viader A, Wright Jin E, Vohra B, Heuckeroth R, Milbrandt J. Differential regional and subtype-specific vulnerability of enteric neurons to mitochondrial dysfunction. PLoS ONE. 2011;6:e27727 pubmed publisher
  • immunohistochemistry; mouse; 1:250; fig 3
Freyer L, Aggarwal V, Morrow B. Dual embryonic origin of the mammalian otic vesicle forming the inner ear. Development. 2011;138:5403-14 pubmed publisher
  • immunohistochemistry; rat; 1:1000; fig 4
Vera G, Castillo M, Cabezos P, Chiarlone A, Martin M, Gori A, et al. Enteric neuropathy evoked by repeated cisplatin in the rat. Neurogastroenterol Motil. 2011;23:370-8, e162-3 pubmed publisher
  • immunohistochemistry - frozen section; human; 1:100
Kapeller J, Möller D, Lasitschka F, Autschbach F, Hovius R, Rappold G, et al. Serotonin receptor diversity in the human colon: Expression of serotonin type 3 receptor subunits 5-HT3C, 5-HT3D, and 5-HT3E. J Comp Neurol. 2011;519:420-32 pubmed publisher
  • immunohistochemistry; pigs ; 1:100; fig 1
Giaroni C, Zanetti E, Giuliani D, Oldrini R, Marchet S, Moro E, et al. Protein kinase C modulates NMDA receptors in the myenteric plexus of the guinea pig ileum during in vitro ischemia and reperfusion. Neurogastroenterol Motil. 2011;23:e91-103 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:50
CHALAZONITIS A, D Autréaux F, Pham T, Kessler J, Gershon M. Bone morphogenetic proteins regulate enteric gliogenesis by modulating ErbB3 signaling. Dev Biol. 2011;350:64-79 pubmed publisher
  • immunohistochemistry - frozen section; zebrafish ; 1:500
Ramachandran R, Reifler A, Parent J, Goldman D. Conditional gene expression and lineage tracing of tuba1a expressing cells during zebrafish development and retina regeneration. J Comp Neurol. 2010;518:4196-212 pubmed publisher
  • immunohistochemistry; guinea pig; 1:1000
Abalo R, Vera G, Rivera A, Moro Rodríguez E, Martín Fontelles M. Postnatal maturation of the gastrointestinal tract: a functional and immunohistochemical study in the guinea-pig ileum at weaning. Neurosci Lett. 2009;467:105-10 pubmed publisher
  • immunocytochemistry; mouse; 1:50; fig 2A
Liu M, Kuan Y, Wang J, Hen R, Gershon M. 5-HT4 receptor-mediated neuroprotection and neurogenesis in the enteric nervous system of adult mice. J Neurosci. 2009;29:9683-99 pubmed publisher
  • immunohistochemistry; human; 1:50
  • immunohistochemistry; guinea pig; 1:50
Hoff S, Zeller F, Von Weyhern C, Wegner M, Schemann M, Michel K, et al. Quantitative assessment of glial cells in the human and guinea pig enteric nervous system with an anti-Sox8/9/10 antibody. J Comp Neurol. 2008;509:356-71 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 10 ug/ml; fig 3
Boyer L, Ghoreishi M, Templeman V, Vallance B, Buchan A, Jevon G, et al. Myenteric plexus injury and apoptosis in experimental colitis. Auton Neurosci. 2005;117:41-53 pubmed
Leggieri A, Attanasio C, Palladino A, Cellerino A, Lucini C, Paolucci M, et al. Identification and Expression of Neurotrophin-6 in the Brain of Nothobranchius furzeri: One More Piece in Neurotrophin Research. J Clin Med. 2019;8: pubmed publisher
Bistoletti M, Caputi V, Baranzini N, Marchesi N, Filpa V, Marsilio I, et al. Antibiotic treatment-induced dysbiosis differently affects BDNF and TrkB expression in the brain and in the gut of juvenile mice. PLoS ONE. 2019;14:e0212856 pubmed publisher
Larsson S, Voss U. Neuroprotective effects of vitamin D on high fat diet- and palmitic acid-induced enteric neuronal loss in mice. BMC Gastroenterol. 2018;18:175 pubmed publisher
Delvalle N, Dharshika C, Morales Soto W, Fried D, Gaudette L, Gulbransen B. Communication Between Enteric Neurons, Glia, and Nociceptors Underlies the Effects of Tachykinins on Neuroinflammation. Cell Mol Gastroenterol Hepatol. 2018;6:321-344 pubmed publisher
Filpa V, Bistoletti M, Caon I, Moro E, Grimaldi A, Moretto P, et al. Changes in hyaluronan deposition in the rat myenteric plexus after experimentally-induced colitis. Sci Rep. 2017;7:17644 pubmed publisher
Pelletier J, Agonsanou H, Delvalle N, Fausther M, Salem M, Gulbransen B, et al. Generation and characterization of polyclonal and monoclonal antibodies to human NTPDase2 including a blocking antibody. Purinergic Signal. 2017;13:293-304 pubmed publisher
Filpa V, Carpanese E, Marchet S, Pirrone C, Conti A, Rainero A, et al. Nitric oxide regulates homeoprotein OTX1 and OTX2 expression in the rat myenteric plexus after intestinal ischemia-reperfusion injury. Am J Physiol Gastrointest Liver Physiol. 2017;312:G374-G389 pubmed publisher
Heanue T, Boesmans W, Bell D, Kawakami K, Vanden Berghe P, Pachnis V. A Novel Zebrafish ret Heterozygous Model of Hirschsprung Disease Identifies a Functional Role for mapk10 as a Modifier of Enteric Nervous System Phenotype Severity. PLoS Genet. 2016;12:e1006439 pubmed publisher
Israel J, Cabelguen J, Le Masson G, Oliet S, Ciofi P. Neonatal testosterone suppresses a neuroendocrine pulse generator required for reproduction. Nat Commun. 2014;5:3285 pubmed publisher
Pacal M, Bremner R. Mapping differentiation kinetics in the mouse retina reveals an extensive period of cell cycle protein expression in post-mitotic newborn neurons. Dev Dyn. 2012;241:1525-44 pubmed publisher
product information
Product Type :
Antibody
Product Name :
HuC/HuD Monoclonal Antibody (16A11), Biotin
Catalog # :
A-21272
Quantity :
100µg
Price :
US 408
Clonality :
Monoclonal
Purity :
purified
Host :
Mouse
Reactivity :
Human
Applications :
ELISA: 1-10 µg/mL, Immunofluorescence: Assay Dependent, Immunohistochemistry: 5-20 µg/mL, Western Blot: 1-5 µg/mL
Species :
Human
Clone :
16A11
Isotype :
IgG2b, kappa
Storage :
-20°C
Description :
Anti-Hu antibodies were originally isolated from a patient with paraneoplastic encephalomyelitis, a rare condition of selective neural tissue injury associated with small cell lung carcinoma. The antibodies bind specifically to antigens present exclusively in neuronal cells and are thus useful as markers of neuronal cells in tissue. The Hu antigen is an RNA-binding protein of the embryonic lethal abnormal visual (Elav) family.
Immunogen :
Human HuC/HuD neuronal protein.
Format :
Lyophilized
Applications w/Dilutions :
ELISA: 1-10 µg/mL, Immunofluorescence: Assay Dependent, Immunohistochemistry: 5-20 µg/mL, Western Blot: 1-5 µg/mL
Aliases :
ELAV (embryonic lethal, abnormal vision, Drosophila)-like 3 (Hu antigen C); ELAV (embryonic lethal, abnormal vision, Drosophila)-like 4 (Hu antigen D); ELAV like neuron-specific RNA binding protein 3; ELAV like neuron-specific RNA binding protein 4; elavC; ELAVL3; elavl3 protein; ELAVL4; ELAV-like protein 3; ELAV-like protein 4; elav-related C; elav-related D; elrc; elrd; embryonic lethal, abnormal vision-related C; embryonic lethal, abnormal vision-related D; etID309927.3; Hu antigen C; Hu antigen D; Hu-antigen C; Hu-antigen D; HUC; HUCL; HUD; id:ibd1248; Paraneoplastic cerebellar degeneration-associated antigen; Paraneoplastic encephalomyelitis antigen HuD; Paraneoplastic limbic encephalitis antigen 21; PLE21; PNEM; Protein elrD; ribonucleoprotein; RNA-binding protein HuD; wu:fb77b03; wu:fc24h01; zHuC
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA