This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
GFP Monoclonal Antibody (11E5)
catalog :
A-11121
quantity :
100µg
price :
280 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
11E5
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, immunohistochemistry - frozen section
citations: 29
Published Application/Species/Sample/DilutionReference
  • immunoprecipitation; scFv; 1:250; fig 5
  • western blot; scFv; 1:5000; fig 5
Li X, Rydzewski N, Hider A, Zhang X, Yang J, Wang W, et al. A molecular mechanism to regulate lysosome motility for lysosome positioning and tubulation. Nat Cell Biol. 2016;18:404-17 pubmed publisher
  • immunocytochemistry; scFv; 1:5000; loading ...; fig s9i
Ulrich F, Carretero Ortega J, Menendez J, Narvaez C, Sun B, Lancaster E, et al. Reck enables cerebrovascular development by promoting canonical Wnt signaling. Development. 2016;143:147-59 pubmed publisher
  • western blot; scFv; 1:1000; fig 1
Dickey A, Pineda V, Tsunemi T, Liu P, Miranda H, Gilmore Hall S, et al. PPAR-δ is repressed in Huntington's disease, is required for normal neuronal function and can be targeted therapeutically. Nat Med. 2016;22:37-45 pubmed publisher
  • western blot; scFv; fig 3
Minniti A, Arrázola M, Bravo Zehnder M, Ramos F, Inestrosa N, Aldunate R. The protein oxidation repair enzyme methionine sulfoxide reductase a modulates A? aggregation and toxicity in vivo. Antioxid Redox Signal. 2015;22:48-62 pubmed publisher
  • western blot; scFv; fig 5
Zhang L, Chen X, Sharma P, Moon M, Sheftel A, Dawood F, et al. HACE1-dependent protein degradation provides cardiac protection in response to haemodynamic stress. Nat Commun. 2014;5:3430 pubmed publisher
  • immunohistochemistry; scFv; 1:500; fig 5
Zhang Y, Raghuwanshi R, Shen W, Montell C. Food experience-induced taste desensitization modulated by the Drosophila TRPL channel. Nat Neurosci. 2013;16:1468-76 pubmed publisher
  • immunohistochemistry; scFv; 1:500; fig 7
Yamamoto S, Bayat V, Bellen H, Tan C. Protein phosphatase 1ß limits ring canal constriction during Drosophila germline cyst formation. PLoS ONE. 2013;8:e70502 pubmed publisher
  • immunohistochemistry - frozen section; scFv; 1:500; fig 7
Wu C, Taneyhill L. Annexin a6 modulates chick cranial neural crest cell emigration. PLoS ONE. 2012;7:e44903 pubmed publisher
  • western blot; scFv; 1:1000; fig 3
Killian R, Flippin J, Herrera C, Almenar Queralt A, Goldstein L. Kinesin light chain 1 suppression impairs human embryonic stem cell neural differentiation and amyloid precursor protein metabolism. PLoS ONE. 2012;7:e29755 pubmed publisher
  • immunohistochemistry; scFv; 1:500; fig 3
Wu C, Jhingory S, Taneyhill L. The tight junction scaffolding protein cingulin regulates neural crest cell migration. Dev Dyn. 2011;240:2309-23 pubmed publisher
  • western blot; scFv; fig 3
Chan D, Somani A, Young A, Massari J, Ohtola J, Sugiyama H, et al. Signal peptide cleavage is essential for surface expression of a regulatory T cell surface protein, leucine rich repeat containing 32 (LRRC32). BMC Biochem. 2011;12:27 pubmed publisher
  • chromatin immunoprecipitation; scFv
Stengel K, Thangavel C, Solomon D, Angus S, Zheng Y, Knudsen E. Retinoblastoma/p107/p130 pocket proteins: protein dynamics and interactions with target gene promoters. J Biol Chem. 2009;284:19265-71 pubmed publisher
  • immunohistochemistry; scFv; 1:300
Batista M, Lewis K. Pax2/8 act redundantly to specify glycinergic and GABAergic fates of multiple spinal interneurons. Dev Biol. 2008;323:88-97 pubmed publisher
  • immunohistochemistry; scFv; fig 2
Bowman A, Catino D, Strong J, Randall W, Kontrogianni Konstantopoulos A, Bloch R. The rho-guanine nucleotide exchange factor domain of obscurin regulates assembly of titin at the Z-disk through interactions with Ran binding protein 9. Mol Biol Cell. 2008;19:3782-92 pubmed publisher
  • western blot; scFv; 1:1000
Scholz I, Still A, Dhenub T, Coday K, Webb M, Barklis E. Analysis of human immunodeficiency virus matrix domain replacements. Virology. 2008;371:322-35 pubmed
  • immunoprecipitation; scFv; fig 1
Frescas D, Guardavaccaro D, Bassermann F, Koyama Nasu R, Pagano M. JHDM1B/FBXL10 is a nucleolar protein that represses transcription of ribosomal RNA genes. Nature. 2007;450:309-13 pubmed
  • western blot; scFv
Morgan P, Pastorekova S, Stuart Tilley A, Alper S, Casey J. Interactions of transmembrane carbonic anhydrase, CAIX, with bicarbonate transporters. Am J Physiol Cell Physiol. 2007;293:C738-48 pubmed
  • western blot; scFv; 1:1000
Herrmann G, Kais S, Hoffbauer J, Shah Hosseini K, Brüggenolte N, Schober H, et al. Conserved interactions of the splicing factor Ntr1/Spp382 with proteins involved in DNA double-strand break repair and telomere metabolism. Nucleic Acids Res. 2007;35:2321-32 pubmed
  • immunoprecipitation; scFv; fig 1
  • western blot; scFv; fig 1
Ao Z, Huang G, Yao H, Xu Z, Labine M, Cochrane A, et al. Interaction of human immunodeficiency virus type 1 integrase with cellular nuclear import receptor importin 7 and its impact on viral replication. J Biol Chem. 2007;282:13456-67 pubmed
  • western blot; scFv; fig 2
Inomoto C, Umemura S, Egashira N, Minematsu T, Takekoshi S, Itoh Y, et al. Granulogenesis in non-neuroendocrine COS-7 cells induced by EGFP-tagged chromogranin A gene transfection: identical and distinct distribution of CgA and EGFP. J Histochem Cytochem. 2007;55:487-93 pubmed
  • western blot; scFv; 1:8000; fig 4
Gao H, Sage T, Osteryoung K. FZL, an FZO-like protein in plants, is a determinant of thylakoid and chloroplast morphology. Proc Natl Acad Sci U S A. 2006;103:6759-64 pubmed
  • western blot; scFv; 0.5 ug/ml; fig 6
Guittaut M, Charpentier S, Normand T, Dubois M, Raimond J, Legrand A. Identification of an internal gene to the human Galectin-3 gene with two different overlapping reading frames that do not encode Galectin-3. J Biol Chem. 2001;276:2652-7 pubmed
Ripp C, Loth J, Petrova I, Linnemannstöns K, Ulepic M, Fradkin L, et al. Drosophila Ror is a nervous system-specific co-receptor for Wnt ligands. Biol Open. 2018;7: pubmed publisher
Schredelseker T, Driever W. Bsx controls pineal complex development. Development. 2018;145: pubmed publisher
Patel A, Yang P, Tinkham M, Pradhan M, Sun M, Wang Y, et al. DNA Conformation Induces Adaptable Binding by Tandem Zinc Finger Proteins. Cell. 2018;173:221-233.e12 pubmed publisher
Marziali F, Bugnon Valdano M, Brunet Avalos C, Moriena L, Cavatorta A, Gardiol D. Interference of HTLV-1 Tax Protein with Cell Polarity Regulators: Defining the Subcellular Localization of the Tax-DLG1 Interaction. Viruses. 2017;9: pubmed publisher
Williams A, White R, Siniard A, Corneveaux J, Huentelman M, Duch C. MECP2 impairs neuronal structure by regulating KIBRA. Neurobiol Dis. 2016;91:284-91 pubmed publisher
Linnemannstöns K, Ripp C, Honemann Capito M, Brechtel Curth K, Hedderich M, Wodarz A. The PTK7-related transmembrane proteins off-track and off-track 2 are co-receptors for Drosophila Wnt2 required for male fertility. PLoS Genet. 2014;10:e1004443 pubmed publisher
Scruggs B, Zhang X, Bowles A, Gold P, Semon J, Fisher Perkins J, et al. Multipotent stromal cells alleviate inflammation, neuropathology, and symptoms associated with globoid cell leukodystrophy in the twitcher mouse. Stem Cells. 2013;31:1523-34 pubmed publisher
product information
Product Type :
Antibody
Product Name :
GFP Monoclonal Antibody (11E5)
Catalog # :
A-11121
Quantity :
100µg
Price :
280 USD
Clonality :
Monoclonal
Purity :
Protein G
Host :
Mouse
Reactivity :
Tag
Applications :
Western Blot: Assay Dependent
Species :
Tag
Clone :
11E5
Isotype :
IgG1
Storage :
-20°C
Description :
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Green Fluorescent Protein (GFP), from the jellyfish Aequorea victoria, has quickly become a versatile marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression, and a powerful research tool for assessing gene expression and subcellular protein distribution in fixed or living cells. GFP is excited by and brightly fluoresces when exposed to UV or blue light. This feature makes it ideal as a marker for use in fluorescence microscopy, cytometry, tagging fusion proteins, and assaying transcriptional regulation from gene promoters in vivo. Numerous GFP variants with enhanced and shifted emission spectra (blue, green, and yellow) have been developed through amino acid substitutions at specific residues.
Immunogen :
The GFP was isolated directly from the jellyfish Aequorea victoria.
Format :
Lyophilized
Applications w/Dilutions :
Western Blot: Assay Dependent
Aliases :
GFP2; Green Fluoresecent Protein; GFP; Turbo GFP; eGFP; GFP tag
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA