eIF2b gamma Polyclonal Antibody
Invitrogen
catalog: PA5-11759

domestic rabbit polyclonal
reactivity: human
application: western blot

quantity: 400 uL
price: US 464.00
to the supplier

eIF2b gamma Monoclonal Antibody (1H3)
Invitrogen
catalog: MA1-078

mouse monoclonal (1H3)
reactivity: human, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, RNA immunoprecipitation

Western blot analysis of eIF2B3 was performed by loading 80µg whole cell lysate onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. Membranes were then probed with a mouse monoclonal antibody recognizing eIF2B3 (Product #MA1-078) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were then washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody (Product #31430) at a dilution of 1:20000 for at least one hour. Membranes were washed and chemiluminescent detection was performed using Super Signal West Dura (Product #34075).

Immunoprecipitation of eIF2B3 was performed on U2OS cell lysates. The antigen:antibody complex was formed by incubating 500µg whole cell lysate with 3µg of mouse monoclonal antibody recognizing eIF2B3 (Product # MA1-078) overnight on a rocking platform at 4°C. The immune-complex was captured on 50µl Protein A/G Plus Agarose (Product #20423). Captured immune-complexes were then washed extensively and proteins eluted with 5X Reducing Sample Loading Dye (Product # 39000). Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to PVDF membrane and blocked with 5% Milk/TBS-0.1%Tween for at least 1 hour. Membranes were then probed with a mouse monoclonal antibody recognizing eIF2B3 (Product # MA1-078) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were washed in TBST and probed with Pierce Clean Blot (Product #21230) at a dilution of 1:1,000 for at least one hour. Membranes were washed and chemiluminescent detection performed using Pierce Super Signal West Dura (Product #34075).
quantity: 100 ug
price: US 491.00
to the supplier

eIF2b gamma Polyclonal Antibody
Invitrogen
catalog: PA5-45822

domestic rabbit polyclonal
reactivity: human
application: western blot

quantity: 100 uL
price: US 464.00
to the supplier

eIF2b gamma Polyclonal Antibody
Invitrogen
catalog: PA5-82871

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 474.00
to the supplier

eIF2b gamma Polyclonal Antibody
Invitrogen
catalog: PA5-82872

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 474.00
to the supplier

eIF2b gamma Polyclonal Antibody
Invitrogen
catalog: PA5-106759

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunocytochemistry

quantity: 100 uL
price: US 474.00
to the supplier