O-linked N-acetylglucosamine (O-GlcNAc) Monoclonal Antibody (RL2)
Invitrogen
catalog: MA1-072

mouse monoclonal (RL2)
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, immunohistochemistry - paraffin section, dot blot, other, western blot knockout validation
citations: 174

quantity: 100 uL
price: US 471.00
to the supplier

O-linked N-acetylglucosamine (O-GlcNAc) Monoclonal Antibody (HGAC85)
Invitrogen
catalog: MA1-076

mouse monoclonal (HGAC85)
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation
citations: 21

quantity: 100 uL
price: US 471.00
to the supplier

O-linked N-acetylglucosamine (O-GlcNAc) Monoclonal Antibody (18B10.C7)
Invitrogen
catalog: MA1-038

mouse monoclonal (18B10.C7)
reactivity: human, dogs
application: western blot, immunoprecipitation
citations: 4

Immunoprecipitation of Glycogen Synthase was performed on HepG2 cells, glucose and serum starved for 36 hours. The antigen:antibody complex was formed by binding 500µg whole cell lysate with 5µg of mouse monoclonal antibody recognizing O-GlcNAc (Product # MA1-038) overnight on a rocking platform at 4°C. The immune-complex was then captured on 50µl Protein A/G Plus Agarose (Product #20463). The beads were washed to remove non-bound material and a low-pH elution buffer was used to dissociate bound antigen from the antibody-bound bead. Samples were then resolved on a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to PVDF membrane and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. Membranes were then probed with a mouse monoclonal antibody recognizing Glycogen Synthase (Product # PA5-17459) at a dilution of 1:1000 overnight rotating at 4°C. Membranes were then washed in TBST and probed with Clean-blot IP detection reagent (Product #21230) at a dilution of 1:2000 for at least one hour. Membranes were washed and chemiluminescent detection was performed using Super Signal West Pico (Product #34087). The image is a Western blot with antibody PA5-17459 showing enrichment of glycogen synthase by O-GlcNAc precipitation. Lane 1 indicates 25 ug of HepG2 whole cell lysate input; Lane 2 indicates the IP elution.

Western blot analysis of O-GlcNAc was performed by loading 10µg of HEK293T or C2C12 whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. Membranes were then probed with a mouse monoclonal antibody recognizing O-GlcNAc (Product #MA1-038) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were then washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody (Product #31430) at a dilution of 1:15000 for at least one hour. Membranes were washed and chemiluminescent detection was performed using Super Signal West Dura (Product #34075). The image is a Western blot with antibody MA1-038 with untreated (lanes 1 and 3) versus treated (lanes 2 and 4) for O-GlcNAc enrichment.
quantity: 100 ug
price: US 451.00
to the supplier

OGT Polyclonal Antibody
Invitrogen
catalog: PA5-22071

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
citations: 3

Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft tissue using PA5-22071 anti-O-GlcNAc transferase antibody at 1:100 dilution.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells using PA5-22071 anti-O-GlcNAc transferase antibody at 1:200 dilution.
quantity: 100 uL
price: US 470.00
to the supplier

OGT Polyclonal Antibody
Invitrogen
catalog: PA5-13654

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
citations: 1

quantity: 400 uL
price: US 474.00
to the supplier

O-linked N-acetylglucosamine (O-GlcNAc) Monoclonal Antibody (9D1.E4)
Invitrogen
catalog: MA1-039

mouse monoclonal (9D1.E4)
reactivity: human
application: western blot, immunoprecipitation
citations: 1

Western blot analysis of O-GlcNAc was performed by loading 10µg of HEK293T or C2C12 whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. Membranes were then probed with a mouse monoclonal antibody recognizing O-GlcNAc (Product #MA1-039) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were then washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody (Product #31430) at a dilution of 1:15000 for at least one hour. Membranes were washed and chemiluminescent detection was performed using Super Signal West Dura (Product #34075). The image is a Western blot with antibody MA1-039 with untreated (lanes 1 and 3) versus treated (lanes 2 and 4) for O-GlcNAc enrichment.

Immunoprecipitation of Glycogen Synthase was performed on HepG2 cells, glucose and serum starved for 36 hours. The antigen:antibody complex was formed by binding 500µg whole cell lysate with 5µg of mouse monoclonal antibody recognizing O-GlcNAc (Product # MA1-039) overnight on a rocking platform at 4°C. The immune-complex was then captured on 50µl Protein A/G Plus Agarose (Product #20463). The beads were washed to remove non-bound material and a low-pH elution buffer was used to dissociate bound antigen from the antibody-bound bead. Samples were then resolved on a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to PVDF membrane and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. Membranes were then probed with a mouse monoclonal antibody recognizing Glycogen Synthase (Product # PA5-17459) at a dilution of 1:1000 overnight rotating at 4°C. Membranes were then washed in TBST and probed with Clean-blot IP detection reagent (Product #21230) at a dilution of 1:2000 for at least one hour. Membranes were washed and chemiluminescent detection was performed using Super Signal West Pico (Product #34087). The image is a Western blot with antibody PA5-17459 showing enrichment of glycogen synthase by O-GlcNAc precipitation. Lane 1 indicates 25 ug of HepG2 whole cell lysate input; Lane 2 indicates the IP elution.
quantity: 100 ug
price: US 471.00
to the supplier

OGT Polyclonal Antibody
Invitrogen
catalog: PA5-83154

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
citations: 1

quantity: 100 uL
price: US 474.00
to the supplier

OGT Polyclonal Antibody
Invitrogen
catalog: PA5-18658

domestic goat polyclonal
reactivity: human, rat
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

Western Blot staining of Rat Pancreas lysate using PA5-18658 at a concentration of 0.05 ug/ml, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.
quantity: 100 ug
price: US 454.00
to the supplier

OGT Polyclonal Antibody
Invitrogen
catalog: PA5-22092

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 464.00
to the supplier

O-GlcNAc Monoclonal Antibody (RL2), PE-Cyanine7, eBioscience
Invitrogen
catalog: 25-9793-42

mouse monoclonal (RL2)
reactivity: human
conjugate: PE/Cy7
application: flow cytometry

quantity: 100 Tests
price: US 426.00
to the supplier

O-GlcNAc Monoclonal Antibody (RL2), PE, eBioscience
Invitrogen
catalog: 12-9793-42

mouse monoclonal (RL2)
reactivity: human
conjugate: PE
application: flow cytometry

quantity: 100 Tests
price: US 424.00
to the supplier

O-GlcNAc Monoclonal Antibody (RL2), Alexa Fluor 647, eBioscience
Invitrogen
catalog: 51-9793-42

mouse monoclonal (RL2)
reactivity: human
conjugate: AF647
application: flow cytometry

quantity: 100 Tests
price: US 426.00
to the supplier

O-GlcNAc Monoclonal Antibody (RL2), Alexa Fluor 488, eBioscience
Invitrogen
catalog: 53-9793-42

mouse monoclonal (RL2)
reactivity: human
conjugate: AF488
application: flow cytometry

quantity: 100 Tests
price: US 425.00
to the supplier

O-GlcNAc Monoclonal Antibody (RL2), Alexa Fluor 488, eBioscience
Invitrogen
catalog: 53-9793-41

mouse monoclonal (RL2)
reactivity: human
conjugate: AF488
application: flow cytometry

quantity: 25 Tests
price: US 193.00
to the supplier

OGT Polyclonal Antibody
Invitrogen
catalog: PA5-83155

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry

quantity: 100 uL
price: US 474.00
to the supplier

OGT Polyclonal Antibody
Invitrogen
catalog: PA5-95319

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

quantity: 100 ug
price: US 464.00
to the supplier

OGT Polyclonal Antibody
Invitrogen
catalog: PA5-96026

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 464.00
to the supplier

OGT Recombinant Rabbit Monoclonal Antibody (JB44-39)
Invitrogen
catalog: MA5-34661

domestic rabbit recombinant (JB44-39)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 474.00
to the supplier

OGT Recombinant Rabbit Monoclonal Antibody (ARC0790)
Invitrogen
catalog: MA5-35376

domestic rabbit recombinant (ARC0790)
reactivity: human, mouse, rat
application: western blot, ELISA

quantity: 100 uL
price: US 474.00
to the supplier

OGT Recombinant Rabbit Monoclonal Antibody (HL1641)
Invitrogen
catalog: MA5-47117

domestic rabbit recombinant (HL1641)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 444.00
to the supplier

OGT Recombinant Rabbit Monoclonal Antibody (HL1642)
Invitrogen
catalog: MA5-47118

domestic rabbit recombinant (HL1642)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 444.00
to the supplier

OGT Recombinant Rabbit Monoclonal Antibody (8G7)
Invitrogen
catalog: MA5-49895

domestic rabbit recombinant (8G7)
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 414.00
to the supplier