LIN28A Polyclonal Antibody
Invitrogen
catalog: PA1-096

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
citations: 4

Immunofluorescent analysis of LIN28 using anti-LIN28 polyclonal antibody (Product# PA1-096) shows specific expression in human embryonal carcinoma NTERA-2 cells (shown in green) but not in control HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA (Product #37525) for 15 minutes at room temperature. Cells were probed with a rabbit polyclonal antibody recognizing LIN28 (Product# PA1-096), at a dilution of 1:200 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goat-anti-rabbit IgG secondary antibody (Product# 35503) at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product# 62249). Images were taken on a Thermo Scientific ArrayScan at 20X.

Western blot analysis of LIN28 was performed by loading 75ug of NCCIT, NTERA-2 and control HeLa whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for 1 hour. Membranes were probed with a rabbit polyclonal antibody recognizing LIN28 (Product # PA1-096) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-rabbit-HRP secondary antibody (Product # 31460) at a dilution of 1:20,000 for one hour. Membranes were washed and chemiluminescent detection performed using Super Signal West Pico (Product #34078).
quantity: 100 ug
price: US 490.00
to the supplier

LIN28A Polyclonal Antibody
Invitrogen
catalog: PA1-096X

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
citations: 5

Western blot analysis of LIN28 was performed by loading 75ug of NCCIT, NTERA-2 and control HeLa whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for 1 hour. Membranes were probed with a rabbit polyclonal antibody recognizing LIN28 (Product # PA1-096) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-rabbit-HRP secondary antibody (Product # 31460) at a dilution of 1:20,000 for one hour. Membranes were washed and chemiluminescent detection performed using Super Signal West Pico (Product #34078).
quantity: 20 uL
price: US 177.00
to the supplier

LIN28A Monoclonal Antibody (14E6-4E6)
Invitrogen
catalog: MA1-016

mouse monoclonal (14E6-4E6)
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
citations: 2

quantity: 100 ug
price: US 440.00
to the supplier

LIN28A Monoclonal Antibody (14E6-4E6), DyLight 650
Invitrogen
catalog: MA1-016-D650

mouse monoclonal (14E6-4E6)
reactivity: human, mouse
conjugate: DyLight 650
application: immunocytochemistry, flow cytometry
citations: 1

quantity: 100 uL
price: US 440.00
to the supplier

LIN28A Monoclonal Antibody (14E6-4E6), DyLight 488
Invitrogen
catalog: MA1-016-D488

mouse monoclonal (14E6-4E6)
reactivity: human, mouse
conjugate: DyLight488
application: immunocytochemistry, flow cytometry

quantity: 100 uL
price: US 399.00
to the supplier

LIN28A Monoclonal Antibody (GT717)
Invitrogen
catalog: MA5-31461

mouse monoclonal (GT717)
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 464.00
to the supplier

LIN28A Polyclonal Antibody
Invitrogen
catalog: PA5-85477

domestic rabbit polyclonal
reactivity: human, mouse, pigs
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 464.00
to the supplier

Phospho-LIN28A (Ser200) Polyclonal Antibody
Invitrogen
catalog: PA5-105696

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunocytochemistry

quantity: 100 uL
price: US 454.00
to the supplier

LIN28A Recombinant Rabbit Monoclonal Antibody (2S1I10)
Invitrogen
catalog: MA5-42929

domestic rabbit recombinant (2S1I10)
reactivity: human, mouse, rat
application: ELISA, immunocytochemistry

quantity: 100 uL
price: US 470.00
to the supplier