Histone H3 Polyclonal Antibody
Invitrogen
catalog: PA5-16183

rabbit polyclonal
reactivity: Chlamydomonas reinhardtii, human, mouse, rat, chicken, cow, zebrafish , fruit fly
application: western blot, immunocytochemistry
citations: 4

quantity: 100µL
price: US 345
to the supplier

Phospho-Histone H3 (Ser10) Polyclonal Antibody
Invitrogen
catalog: PA5-17869

rabbit polyclonal
reactivity: Saccharomyces cerevisiae S288c, human, mouse, rat, fruit fly, common platanna
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 4

Immunofluorescent analysis of Phospho-Histone H3 pSer10 (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed without (left panel) or with (right panel) a Phospho-Histone H3 polyclonal antibody (Product # PA5-17869) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan or a ToxInsight Instrument at 20X magnification.

Immunohistochemistry was performed on paraffin-embedded human breast carcinoma. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer for 10 minutes at 95C. Following antigen-retrieval, tissues were incubated in 3% hydrogen peroxide for 10 minutes, washed, and then blocked in 5% normal goat serum in TBST for 1 hour at room temperature. Tissues were stained with a Phospho-Histone H3 polyclonal antibody (Product # PA5-17869) at a dilution of 1:200 overnight at 4C. Tissues were washed extensively with TBS + 0.1% Tween-20 and detection was performed using a HRP-conjugated secondary reagent followed by DAB substrate.

Flow cytometric analysis of Phospho-Histone H3 pSer10 on Ramos cells left untreated (blue histogram) or treated with serum/calyculin (green histogram). Cells were harvested, fixed with formaldehyde, permeabilized with methanol, and incubated with a Phospho-Histone H3 pSer10 polyclonal antibody (Product # PA5-17869) at a dilution of 1:50 for 1 hour at room temperature. As a negative control, serum/calyculin-treated cells were stained with an isotype control (red histogram). Following staining with either PA5-17869 or isotype control, cells were stained with a fluorescently-conjugated anti-rabbit IgG secondary antibody and analyzed on a flow cytometer.
quantity: 100µL
price: US 345
to the supplier

Histone H3 Monoclonal Antibody (E.960.2)
Invitrogen
catalog: MA5-15150

rabbit monoclonal (E.960.2)
reactivity: Chinese hamster, human, mouse, rat, dog, cow, pig, zebrafish , fruit fly
application: western blot
citations: 3

quantity: 100µL
price: US 345
to the supplier

Acetyl-Histone H3 (Lys9) Monoclonal Antibody (J.924.2)
Invitrogen
catalog: MA5-11195

rabbit monoclonal (J.924.2)
reactivity: common tobacco, human, mouse, rat, zebrafish , Caenorhabditis elegans
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
citations: 1

Immunoprecipitation of acetylated Histone H3 (Lys9) was performed using whole cell lysates from cells left untreated (DMSO only) or cells treated with 0.3uM or 3uM Trichostatin A (TSA) for 16 hours. Antigen-antibody complexes were formed by incubating 500ug of the indicated lysate with 3ug of an Acetyl Lysine monoclonal antibody (Product # MA1-2021) overnight on a rocking platform at 4°C. The immune complexes were captured on 50ul Protein A/G Agarose (Product # 20421), washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer (Product # 39000). Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with an Acetyl-Histone H3 (Lys9) monoclonal antibody (Product # MA5-11195) at a dilution of 1:1000 overnight rotating at 4°C, washed in TBST, and probed with Clean-blot IP Detection Reagent (Product # 21230) at a dilution of 1:2000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34087).
quantity: 100µL
price: US 345
to the supplier

Tri-Methyl-Histone H3 (Lys4) Monoclonal Antibody (G.532.8)
Invitrogen
catalog: MA5-11199

rabbit monoclonal (G.532.8)
reactivity: Saccharomyces cerevisiae S288c, human, mouse, rat, zebrafish , fruit fly, common platanna
application: western blot, immunohistochemistry, immunocytochemistry, chromatin immunoprecipitation, immunohistochemistry - paraffin section
citations: 1

quantity: 100µL
price: US 345
to the supplier

Tri-Methyl-Histone H3 (Lys27) Monoclonal Antibody (G.299.10)
Invitrogen
catalog: MA5-11198

rabbit monoclonal (G.299.10)
reactivity: human, mouse, rat, zebrafish
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

quantity: 100µL
price: US 390
to the supplier

Di-Methyl-Histone H3 (Lys36) Monoclonal Antibody (T.571.7)
Invitrogen
catalog: MA5-14867

rabbit monoclonal (T.571.7)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

quantity: 100µL
price: US 345
to the supplier

Di-Methyl-Histone H3 (Lys4) Monoclonal Antibody (T.767.5)
Invitrogen
catalog: MA5-14977

rabbit monoclonal (T.767.5)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

quantity: 100µL
price: US 345
to the supplier

Phospho-Histone H3 (Ser10) Monoclonal Antibody (K.872.3)
Invitrogen
catalog: MA5-15220

mouse monoclonal (K.872.3)
reactivity: human, mouse, rat
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

quantity: 100µL
price: US 345
to the supplier

Di-Methyl-Histone H3 (Lys9) Polyclonal Antibody
Invitrogen
catalog: PA5-16195

rabbit polyclonal
reactivity: human, mouse, rat, fruit fly
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

quantity: 100µL
price: US 345
to the supplier

Tri-Methyl-Histone H3 (Lys36) Polyclonal Antibody
Invitrogen
catalog: PA5-17109

rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

quantity: 100µL
price: US 345
to the supplier

Phospho-Histone H3 (Ser28) Polyclonal Antibody
Invitrogen
catalog: PA5-17318

rabbit polyclonal
reactivity: Chinese hamster, human, mouse, rat, chicken, cow, zebrafish , fruit fly, common platanna
modification: phosphorylated
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry

quantity: 100µL
price: US 345
to the supplier

Phospho-Histone H3 (Thr11) Polyclonal Antibody
Invitrogen
catalog: PA5-17360

rabbit polyclonal
reactivity: human, mouse, rat, common platanna
modification: phosphorylated
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry

quantity: 100µL
price: US 345
to the supplier

Methyl-Histone H3 (Lys4) Polyclonal Antibody
Invitrogen
catalog: PA5-17418

rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunoprecipitation

quantity: 100µL
price: US 345
to the supplier

Tri-Methyl-Histone H3 (Lys4) Polyclonal Antibody
Invitrogen
catalog: PA5-17420

rabbit polyclonal
reactivity: human, mouse, rat, zebrafish , common platanna
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

quantity: 100µL
price: US 345
to the supplier

Acetyl-Histone H3 (Lys18) Polyclonal Antibody
Invitrogen
catalog: PA5-17801

rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

quantity: 100µL
price: US 345
to the supplier

Acetyl-Histone H3 (Lys9, Lys14) Polyclonal Antibody
Invitrogen
catalog: PA5-16194

rabbit polyclonal
reactivity: human, mouse, rat, zebrafish , Caenorhabditis elegans
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

quantity: 100µL
price: US 390
to the supplier

Phospho-Histone H3 (Thr3) Polyclonal Antibody
Invitrogen
catalog: PA5-17502

rabbit polyclonal
reactivity: human, mouse, rat
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

quantity: 100µL
price: US 345
to the supplier

Mono-Methyl-Histone H3 (Arg128) Recombinant Rabbit Monoclonal Antibody
Invitrogen
catalog: MA5-33158

rabbit recombinant
reactivity: human, mouse
application: western blot, ELISA

quantity: 100µL
price: US 375
to the supplier