RAGE Polyclonal Antibody
Invitrogen
catalog: PA1-075
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 9

Immunofluorescent analysis of RAGE in NIH-3T3 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a RAGE polyclonal antibody (Product # PA1-075) at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). RAGE staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

Immunohistochemistry was performed on normal biopsies of deparaffinized Mouse lymph node tissue . To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal antibody recognizing RAGE (PA1-075) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
quantity: 100 ug
price: US 471.00
to the supplier
RAGE Polyclonal Antibody
Invitrogen
catalog: PA5-78736
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
quantity: 100 ug
price: US 430.00
to the supplier
RAGE Polyclonal Antibody
Invitrogen
catalog: PA5-78735
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
quantity: 100 ug
price: US 430.00
to the supplier
RAGE Recombinant Rabbit Monoclonal Antibody (005)
Invitrogen
catalog: MA5-30062
domestic rabbit recombinant (5)
reactivity: human, mouse, rat
application: western blot, ELISA
quantity: 100 uL
price: US 410.00
to the supplier
RAGE Polyclonal Antibody
Invitrogen
catalog: PA5-89548
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA
quantity: 100 uL
price: US 464.00
to the supplier
RAGE Recombinant Rabbit Monoclonal Antibody (JF0975)
Invitrogen
catalog: MA5-41206
domestic rabbit recombinant (JF0975)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 470.00
to the supplier
RAGE Monoclonal Antibody (5C6C1)
Invitrogen
catalog: MA5-49253
mouse monoclonal (5C6C1)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section
quantity: 100 ug
price: US 404.00
to the supplier