Nanog Polyclonal Antibody
Invitrogen
catalog: PA1-097

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 18

Immunofluorescent analysis of Nanog using anti-Nanog polyclonal antibody (Product# PA1-097) shows nuclear localization in the human iPS cell line HEL 11.4. Nanog staining (red) and an overlay image of Nanog with DAPI (pink) is shown. HEL-11.4 cells were grown on matrigel coated chamber slides, fixed with formaldehyde and permeabilized with 0.1% Triton X-100 in TBS for 15 minutes at room temperature. The cells were probed with a rabbit polyclonal antibody recognizing Nanog (Product# PA1-097) at a dilution of 1:100 overnight at 4 ºC, washed with PBS and incubated with a fluorescein conjugated secondary antibody at a dilution of 1:100 for 60 minutes at room temperature. Images were taken on at 20X magnification.

Immunofluorescent analysis of Nanog using anti-Nanog polyclonal antibody (Product# PA1-097) shows nuclear localization in the human embryonic stem cell line H9. Nanog staining (red) and an overlay image of Nanog with DAPI (pink) is shown. H9 cells were grown on matrigel coated chamber slides, fixed with formaldehyde and permeabilized with 0.1% Triton X-100 in TBS for 15 minutes at room temperature. The cells were probed with a rabbit polyclonal antibody recognizing Nanog (Product# PA1-097) at a dilution of 1:100 overnight at 4 ºC, washed with PBS and incubated with a fluorescein conjugated secondary antibody at a dilution of 1:100 for 60 minutes at room temperature. Images were taken on at 20X magnification.

Western blot analysis of Nanog was performed by loading 80ug of NCCIT, NTERA-2 and negative control HeLa whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for 1 hour. Membranes were probed with a rabbit polyclonal antibody recognizing Nanog (Product # PA1-097) at a dilution of 1:2000 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-rabbit-HRP secondary antibody (Product # 31460) at a dilution of 1:20,000 for one hour. Membranes were washed and chemiluminescent detection performed using Pierce Super Signal West Pico (Product #34078).
quantity: 100 ug
price: US 490.00
to the supplier

Nanog Polyclonal Antibody
Invitrogen
catalog: PA1-097X

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 17

Immunofluorescent analysis of Nanog using anti-Nanog polyclonal antibody (Product# PA1-097) shows specific expression in human embryonal carcinoma NTERA-2 cells (shown in green) but not in negative control HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA (Product #37525) for 15 minutes at room temperature. Cells were probed with a rabbit polyclonal antibody recognizing Nanog (Product# PA1-097), at a dilution of 1:200 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goat-anti-rabbit IgG secondary antibody (Product# 35503) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DY-547 phalloidin, nuclei (blue) were stained with Hoechst 33342 dye (Product# 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.

Western blot analysis of Nanog was performed by loading 80ug of NCCIT, NTERA-2 and negative control HeLa whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for 1 hour. Membranes were probed with a rabbit polyclonal antibody recognizing Nanog (Product # PA1-097) at a dilution of 1:2000 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-rabbit-HRP secondary antibody (Product # 31460) at a dilution of 1:20,000 for one hour. Membranes were washed and chemiluminescent detection performed using Pierce Super Signal West Pico (Product #34078).

Immunofluorescent analysis of Nanog using anti-Nanog polyclonal antibody (Product# PA1-097) shows nuclear localization in the human embryonic stem cell line H9. Nanog staining (red) and an overlay image of Nanog with DAPI (pink) is shown. H9 cells were grown on matrigel coated chamber slides, fixed with formaldehyde and permeabilized with 0.1% Triton X-100 in TBS for 15 minutes at room temperature. The cells were probed with a rabbit polyclonal antibody recognizing Nanog (Product# PA1-097) at a dilution of 1:100 overnight at 4 ºC, washed with PBS and incubated with a fluorescein conjugated secondary antibody at a dilution of 1:100 for 60 minutes at room temperature. Images were taken on at 20X magnification.
quantity: 20 uL
price: US 187.00
to the supplier

Nanog Monoclonal Antibody (hNanog.2), eBioscience
Invitrogen
catalog: 14-5768-80

mouse monoclonal (hNanog.2)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 14

quantity: 25 ug
price: US 187.00
to the supplier

Nanog Monoclonal Antibody (hNanog.2), eBioscience
Invitrogen
catalog: 14-5768-82

mouse monoclonal (hNanog.2)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 15

quantity: 100 ug
price: US 440.00
to the supplier

Nanog Monoclonal Antibody (23D2-3C6)
Invitrogen
catalog: MA1-017

mouse monoclonal (23D2-3C6)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry
citations: 11

quantity: 100 ug
price: US 414.00
to the supplier

Nanog Polyclonal Antibody, PeproTech
Invitrogen
catalog: 500-P236-100UG

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
citations: 7

quantity: 100 ug
price: US 304.00
to the supplier

Nanog Polyclonal Antibody, PeproTech
Invitrogen
catalog: 500-P236-1MG

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
citations: 7

quantity: 1 mg
price: US 2070.00
to the supplier

Nanog Polyclonal Antibody, PeproTech
Invitrogen
catalog: 500-P236-50UG

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
citations: 7

quantity: 50 ug
price: US 238.00
to the supplier

Nanog Polyclonal Antibody
Invitrogen
catalog: PA5-18406

domestic goat polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry
citations: 2

Immunofluorescent staining (green) parts of a colony of induced pluriform stem cells derived from Human Keratinocytes using PA5-18406 at a concentration of 5ug/ml.

Western Blot staining of Human Ovary lysate using PA5-18406 at a concentration of 0.03 ug/ml, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.
quantity: 100 ug
price: US 474.00
to the supplier

Nanog Polyclonal Antibody
Invitrogen
catalog: PA5-20889

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
citations: 3

Immunohistochemical staining of human spleen tissue using PA5-20889 anti-NANOG antibody at a concentration of 5 ug/mL.

Western blot analysis of human spleen tissue lysate using PA5-20889 anti-NANOG antibody at (A) 1 and (B) 2 ug/mL.
quantity: 100 ug
price: US 470.00
to the supplier

Nanog Polyclonal Antibody, PE
Invitrogen
catalog: PA5-46891

domestic goat polyclonal
reactivity: human
conjugate: PE
application: flow cytometry
citations: 2

quantity: 100 Tests
price: US 392.00
to the supplier

Nanog Polyclonal Antibody
Invitrogen
catalog: PA5-85110

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 2

quantity: 100 uL
price: US 464.00
to the supplier

Nanog Polyclonal Antibody
Invitrogen
catalog: PA1-41577

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunocytochemistry, flow cytometry
citations: 1

quantity: 100 ug
price: US 474.00
to the supplier

Nanog Monoclonal Antibody (1E6C4)
Invitrogen
catalog: MA5-15469

mouse monoclonal (1E6C4)
reactivity: human
application: western blot, ELISA, immunocytochemistry
citations: 1

Confocal immunofluorescence analysis of NTERA-2 cells (left) and HeLa cells (right) using Nanog antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin.
quantity: 100 uL
price: US 552.00
to the supplier

Phospho-Nanog (Ser71) Polyclonal Antibody
Invitrogen
catalog: PA5-13078

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: immunocytochemistry, dot blot

quantity: 400 uL
price: US 464.00
to the supplier

Nanog Polyclonal Antibody
Invitrogen
catalog: PA5-18678

domestic goat polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemical staining of paraffin embedded of Human Testis using PA5-18678 at a concentration of 2 ug/ml. The tissue was processed by steamed antigen retrieval with citrate buffer pH 6 and stained with AP.

Western Blot staining of nuclear HeLa cell lysate using PA5-18678 at a concentration of 0.05 ug/ml, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.
quantity: 100 ug
price: US 464.00
to the supplier

Phospho-Nanog (Ser285) Polyclonal Antibody
Invitrogen
catalog: PA5-13079

domestic rabbit polyclonal
reactivity: human
modification: phosphorylated
application: dot blot

quantity: 400 uL
price: US 464.00
to the supplier

Nanog Monoclonal Antibody (23D2-3C6), DyLight 488
Invitrogen
catalog: MA1-017-D488

mouse monoclonal (23D2-3C6)
reactivity: human
conjugate: DyLight488
application: immunocytochemistry, flow cytometry

quantity: 100 uL
price: US 409.00
to the supplier

Nanog Monoclonal Antibody (23D2-3C6), DyLight 650
Invitrogen
catalog: MA1-017-D650

mouse monoclonal (23D2-3C6)
reactivity: human
conjugate: DyLight 650
application: immunocytochemistry

quantity: 100 uL
price: US 440.00
to the supplier

Nanog Monoclonal Antibody (GT3312)
Invitrogen
catalog: MA5-31459

mouse monoclonal (GT3312)
reactivity: human, mouse
application: western blot, immunocytochemistry, flow cytometry

quantity: 100 uL
price: US 464.00
to the supplier

Nanog Recombinant Rabbit Monoclonal Antibody (SC05-70)
Invitrogen
catalog: MA5-32198

domestic rabbit recombinant (SC05-70)
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry

quantity: 100 uL
price: US 474.00
to the supplier

Nanog Polyclonal Antibody, Biotin, PeproTech
Invitrogen
catalog: 500-P236BT-25UG

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA

quantity: 25 ug
price: US 238.00
to the supplier

Nanog Polyclonal Antibody, Biotin, PeproTech
Invitrogen
catalog: 500-P236BT-50UG

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA

quantity: 50 ug
price: US 304.00
to the supplier

Nanog Recombinant Rabbit Monoclonal Antibody (2G6)
Invitrogen
catalog: MA5-50178

domestic rabbit recombinant (2G6)
reactivity: human
application: ELISA, flow cytometry

quantity: 100 uL
price: US 414.00
to the supplier

Nanog Polyclonal Antibody, Biotin, PeproTech
Invitrogen
catalog: 500-P236BT-500UG

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: western blot, ELISA

quantity: 500 ug
price: US 2070.00
to the supplier

Nanog Recombinant Rabbit Monoclonal Antibody (4T1C9)
Invitrogen
catalog: MA5-55217

domestic rabbit recombinant (4T1C9)
reactivity: human, mouse, rat
application: western blot, ELISA, immunocytochemistry

quantity: 100 uL
price: US 419.00
to the supplier