MHC Class II I-Ab Monoclonal Antibody (AF6-120.1), Super Bright 780, eBioscience™ | Invitrogen 78-5320-82 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

MHC Class II I-Ab Monoclonal Antibody (AF6-120.1), Super Bright 780, eBioscience™

catalog :

78-5320-82

quantity :

100 µg

price :

US 400

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AF6-120.1

reactivity :

mouse

application :

flow cytometry

more info or order :

Invitrogen product webpage

citations: 10

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; loading ...; fig 4c	Kretschmann S, Herda S, Bruns H, Russ J, van der Meijden E, Schlötzer Schrehardt U, et al. Chaperone protein HSC70 regulates intercellular transfer of Y chromosome antigen DBY. J Clin Invest. 2019;129:2952-2963 pubmed publisher
	Celorrio M, Abellanas M, Rhodes J, Goodwin V, Moritz J, Vadivelu S, et al. Gut microbial dysbiosis after traumatic brain injury modulates the immune response and impairs neurogenesis. Acta Neuropathol Commun. 2021;9:40 pubmed publisher
	Velletri T, Huang Y, Wang Y, Li Q, Hu M, Xie N, et al. Loss of p53 in mesenchymal stem cells promotes alteration of bone remodeling through negative regulation of osteoprotegerin. Cell Death Differ. 2021;28:156-169 pubmed publisher
	Giles D, Duncker P, Wilkinson N, Washnock Schmid J, Segal B. CNS-resident classical DCs play a critical role in CNS autoimmune disease. J Clin Invest. 2018;128:5322-5334 pubmed publisher
	Nalawade S, Ji N, Raphael I, Pratt A, Kraig E, Forsthuber T. Aire is not essential for regulating neuroinflammatory disease in mice transgenic for human autoimmune-diseases associated MHC class II genes HLA-DR2b and HLA-DR4. Cell Immunol. 2018;331:38-48 pubmed publisher
	Nyhoff L, Clark E, Barron B, Bonami R, Khan W, Kendall P. Bruton's Tyrosine Kinase Is Not Essential for B Cell Survival beyond Early Developmental Stages. J Immunol. 2018;200:2352-2361 pubmed publisher
	Dudeck J, Medyukhina A, Fröbel J, Svensson C, Kotrba J, Gerlach M, et al. Mast cells acquire MHCII from dendritic cells during skin inflammation. J Exp Med. 2017;214:3791-3811 pubmed publisher
	Ono Y, Pérez Gutiérrez A, Nakao T, Dai H, Camirand G, Yoshida O, et al. Graft-infiltrating PD-L1hi cross-dressed dendritic cells regulate antidonor T cell responses in mouse liver transplant tolerance. Hepatology. 2018;67:1499-1515 pubmed publisher
	Zhao T, Bernstein K, Fang J, Shen X. Angiotensin-converting enzyme affects the presentation of MHC class II antigens. Lab Invest. 2017;97:764-771 pubmed publisher
	Bracamonte Baran W, Florentin J, Zhou Y, Jankowska Gan E, Haynes W, Zhong W, et al. Modification of host dendritic cells by microchimerism-derived extracellular vesicles generates split tolerance. Proc Natl Acad Sci U S A. 2017;114:1099-1104 pubmed publisher

product information

Product Type :

Antibody

Product Name :

MHC Class II I-Ab Monoclonal Antibody (AF6-120.1), Super Bright 780, eBioscience™

Catalog # :

78-5320-82

Quantity :

100 µg

Price :

US 400

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Mouse

Reactivity :

Mouse

Applications :

Flow Cytometry: 1.0 µg/test

Species :

Mouse

Clone :

AF6-120.1

Isotype :

IgG2a, kappa

Storage :

4° C, store in dark, DO NOT FREEZE!

Description :

I-Ab (H2-Ab1, histocompatibility 2, class II antigen A, beta 1) is an MHC class II heterodimer molecule of non-covalently associated alpha (31-34 kDa) and beta (26-29 kDa) chains. Major histocompatibility complex class II antigen presentation requires the participation of lysosomal proteases in two convergent processes. First, the antigens endocytosed by the antigen presenting cells must be broken down into antigenic peptides. Second, class II molecules are synthesized with their peptide-binding site blocked by invariant chain (Ii), and they aquire the capacity to bind antigens only after Ii has been degraded in the compartments where peptides reside. MHC class II molecules present exogenously derived antigen to CD4+ T lymphocytes, which are usually T helper cells. CD4 interacts with non-polymorphic residues of MHC class II. H2-Ab1 heterodimer chains alpha (DQA) and beta (DQB) are both anchored in the membrane. H2-Ab1 plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to 4 different molecules. Typing for polymorphisms is routinely done for bone marrow transplantation.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 1.0 µg/test

Aliases :

Abeta; AI845868; CELIAC1; DADB-249P12.2; H-2 class II histocompatibility antigen, A beta chain; H-2Ab; H2-Ab; H2-Ab1; H2Eb; H-2Eb; H2-iabeta; histocompatibility 2, class II antigen A, beta 1; HLA-DQB; Ia2; Ia-2; Ia4; Ia-4; IAb; I-Abeta; IDDM1; major histocompatibility complex class II beta chain; MGC163794; MGC163796; MHC class II antigen A beta; MHC class II H2-IA-beta-psi; response to metastatic cancers 1; Rmcs1

more info or order :

Invitrogen product webpage