product summary
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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
F4/80 Monoclonal Antibody (BM8), Super Bright 780, eBioscience™
catalog :
78-4801-82
quantity :
100 µg
price :
US 428
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
BM8
reactivity :
mouse
application :
immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 153
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig 2d
Erlandsson M, Erdogan S, Was xe9 n C, Andersson K, Silfversw xe4 rd S, Pullerits R, et al. IGF1R signalling is a guardian of self-tolerance restricting autoantibody production. Front Immunol. 2022;13:958206 pubmed publisher
  • flow cytometry; mouse; loading ...
Amaral E, Foreman T, Namasivayam S, Hilligan K, Kauffman K, Barbosa Bomfim C, et al. GPX4 regulates cellular necrosis and host resistance in Mycobacterium tuberculosis infection. J Exp Med. 2022;219: pubmed publisher
  • flow cytometry; mouse; loading ...; fig s3
Keller E, Dvorina N, Jørgensen T. Spontaneous CD4+ T Cell Activation and Differentiation in Lupus-Prone B6.Nba2 Mice Is IFNAR-Independent. Int J Mol Sci. 2022;23: pubmed publisher
  • flow cytometry; mouse; loading ...; fig s3j
Bhattacharjee O, Ayyangar U, Kurbet A, Lakshmanan V, Palakodeti D, Ginhoux F, et al. Epithelial-Macrophage Crosstalk Initiates Sterile Inflammation in Embryonic Skin. Front Immunol. 2021;12:718005 pubmed publisher
  • immunocytochemistry; mouse; fig s1b
Zhang M, Pan X, Fujiwara K, Jurcak N, Muth S, Zhou J, et al. Pancreatic cancer cells render tumor-associated macrophages metabolically reprogrammed by a GARP and DNA methylation-mediated mechanism. Signal Transduct Target Ther. 2021;6:366 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4c
Zhao Y, Sun J, Li Y, Zhou X, Zhai W, Wu Y, et al. Tryptophan 2,3-dioxygenase 2 controls M2 macrophages polarization to promote esophageal squamous cell carcinoma progression via AKT/GSK3β/IL-8 signaling pathway. Acta Pharm Sin B. 2021;11:2835-2849 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4a
Petermann M, Orfanos Z, Sellau J, Gharaibeh M, Lotter H, Fleischer B, et al. CCR2 Deficiency Impairs Ly6Clo and Ly6Chi Monocyte Responses in Orientia tsutsugamushi Infection. Front Immunol. 2021;12:670219 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s2g
  • immunohistochemistry; mouse; 1:500; loading ...; fig 2i
Liu M, Rao H, Liu J, Li X, Feng W, Gui L, et al. The histone methyltransferase SETD2 modulates oxidative stress to attenuate experimental colitis. Redox Biol. 2021;43:102004 pubmed publisher
  • flow cytometry; mouse; loading ...
Jhala G, Selck C, Chee J, Kwong C, Pappas E, Thomas H, et al. Tolerance to Proinsulin-1 Reduces Autoimmune Diabetes in NOD Mice. Front Immunol. 2021;12:645817 pubmed publisher
  • flow cytometry; mouse
Chu A, Kok S, TSUI J, Lin M, Aguirre B, Wadehra M. Epithelial membrane protein 2 (Emp2) modulates innate immune cell population recruitment at the maternal-fetal interface. J Reprod Immunol. 2021;145:103309 pubmed publisher
  • flow cytometry; mouse; loading ...
Zhang Y, Liu J, Wang X, Zhang J, Xie C. Extracellular vesicle-encapsulated microRNA-23a from dorsal root ganglia neurons binds to A20 and promotes inflammatory macrophage polarization following peripheral nerve injury. Aging (Albany NY). 2021;13:6752-6764 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2a, 7a
Weng X, Zhao H, Guan Q, Shi G, Feng S, Gleave M, et al. Clusterin regulates macrophage expansion, polarization and phagocytic activity in response to inflammation in the kidneys. Immunol Cell Biol. 2021;99:274-287 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1b
Uhl B, Braun C, Dominik J, Luft J, Canis M, Reichel C. A Novel Experimental Approach for In Vivo Analyses of the Salivary Gland Microvasculature. Front Immunol. 2020;11:604470 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3c
Ebelt N, Zuniga E, Marzagalli M, Zamloot V, Blazar B, Salgia R, et al. Salmonella-Based Therapy Targeting Indoleamine 2,3-Dioxygenase Restructures the Immune Contexture to Improve Checkpoint Blockade Efficacy. Biomedicines. 2020;8: pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig s1-1e
Crespo M, González Terán B, Nikolic I, Mora A, Folgueira C, Rodriguez E, et al. Neutrophil infiltration regulates clock-gene expression to organize daily hepatic metabolism. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; fig s3
Azcutia V, Kelm M, Luissint A, Boerner K, Flemming S, Quirós M, et al. Neutrophil expressed CD47 regulates CD11b/CD18-dependent neutrophil transepithelial migration in the intestine in vivo. Mucosal Immunol. 2020;: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1c
Park M, Kim H, Lee H, Zabel B, Bae Y. Novel CD11b+Gr-1+Sca-1+ myeloid cells drive mortality in bacterial infection. Sci Adv. 2020;6:eaax8820 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s1
Campuzano A, Castro López N, Martinez A, Olszewski M, Ganguly A, Leopold Wager C, et al. CARD9 Is Required for Classical Macrophage Activation and the Induction of Protective Immunity against Pulmonary Cryptococcosis. MBio. 2020;11: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3b
Mei Y, Wang Z, Zhang Y, Wan T, Xue J, He W, et al. FA-97, a New Synthetic Caffeic Acid Phenethyl Ester Derivative, Ameliorates DSS-Induced Colitis Against Oxidative Stress by Activating Nrf2/HO-1 Pathway. Front Immunol. 2019;10:2969 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1g
Park C, Kehrl J. An integrin/MFG-E8 shuttle loads HIV-1 viral-like particles onto follicular dendritic cells in mouse lymph node. elife. 2019;8: pubmed publisher
  • flow cytometry; mouse; loading ...; fig s3a
Chu C, Murdock M, Jing D, Won T, Chung H, Kressel A, et al. The microbiota regulate neuronal function and fear extinction learning. Nature. 2019;574:543-548 pubmed publisher
  • flow cytometry; mouse
Choi W, Kim H, Kim M, Cinar R, Yi H, Eun H, et al. Glutamate Signaling in Hepatic Stellate Cells Drives Alcoholic Steatosis. Cell Metab. 2019;30:877-889.e7 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s1d
Loh J, Xu S, Huo J, Kim S, Wang Y, Lam K. Dok3-protein phosphatase 1 interaction attenuates Card9 signaling and neutrophil-dependent antifungal immunity. J Clin Invest. 2019;129:2717-2729 pubmed publisher
  • flow cytometry; mouse; 1:10; loading ...; fig 5h
Kobayakawa K, Ohkawa Y, Yoshizaki S, Tamaru T, Saito T, Kijima K, et al. Macrophage centripetal migration drives spontaneous healing process after spinal cord injury. Sci Adv. 2019;5:eaav5086 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 4a
  • flow cytometry; mouse; 1:200; loading ...; fig 4b
Kuriakose J, Redecke V, Guy C, Zhou J, Wu R, Ippagunta S, et al. Patrolling monocytes promote the pathogenesis of early lupus-like glomerulonephritis. J Clin Invest. 2019;129:2251-2265 pubmed publisher
  • flow cytometry; mouse; 1:300; loading ...; fig e5a, e5b
Jacome Galarza C, Percin G, Muller J, Mass E, Lazarov T, Eitler J, et al. Developmental origin, functional maintenance and genetic rescue of osteoclasts. Nature. 2019;568:541-545 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig s1a
Lee J, Stone M, Porrett P, Thomas S, Komar C, Li J, et al. Hepatocytes direct the formation of a pro-metastatic niche in the liver. Nature. 2019;567:249-252 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4d
Salerno F, Guislain A, Freen van Heeren J, Nicolet B, Young H, Wolkers M. Critical role of post-transcriptional regulation for IFN-γ in tumor-infiltrating T cells. Oncoimmunology. 2019;8:e1532762 pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 3b
  • flow cytometry; mouse; loading ...; fig 3a
Liu M, O Connor R, Trefely S, Graham K, Snyder N, Beatty G. Metabolic rewiring of macrophages by CpG potentiates clearance of cancer cells and overcomes tumor-expressed CD47-mediated 'don't-eat-me' signal. Nat Immunol. 2019;20:265-275 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3g
Düsedau H, Kleveman J, Figueiredo C, Biswas A, Steffen J, Kliche S, et al. p75NTR regulates brain mononuclear cell function and neuronal structure in Toxoplasma infection-induced neuroinflammation. Glia. 2019;67:193-211 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s2
Kaplanov I, Carmi Y, Kornetsky R, Shemesh A, Shurin G, Shurin M, et al. Blocking IL-1β reverses the immunosuppression in mouse breast cancer and synergizes with anti-PD-1 for tumor abrogation. Proc Natl Acad Sci U S A. 2019;116:1361-1369 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2
Zhou J, Li J, Yu Y, Liu Y, Li H, Liu Y, et al. Mannan-binding lectin deficiency exacerbates sterile liver injury in mice through enhancing hepatic neutrophil recruitment. J Leukoc Biol. 2019;105:177-186 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2a
Humblet Baron S, Barber J, Roca C, Lenaerts A, Koni P, Liston A. Murine myeloproliferative disorder as a consequence of impaired collaboration between dendritic cells and CD4 T cells. Blood. 2018;: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1b
Huang L, Nazarova E, Tan S, Liu Y, Russell D. Growth of Mycobacterium tuberculosis in vivo segregates with host macrophage metabolism and ontogeny. J Exp Med. 2018;215:1135-1152 pubmed publisher
  • flow cytometry; mouse; 1:600; loading ...; fig 1a
Soncin I, Sheng J, Chen Q, Foo S, Duan K, Lum J, et al. The tumour microenvironment creates a niche for the self-renewal of tumour-promoting macrophages in colon adenoma. Nat Commun. 2018;9:582 pubmed publisher
Korir M, Doster R, Lu J, Guevara M, Spicer S, Moore R, et al. Streptococcus agalactiae cadD alleviates metal stress and promotes intracellular survival in macrophages and ascending infection during pregnancy. Nat Commun. 2022;13:5392 pubmed publisher
Weber A, Yang X, Mennillo E, Ding J, Watrous J, Jain M, et al. Lactational delivery of Triclosan promotes non-alcoholic fatty liver disease in newborn mice. Nat Commun. 2022;13:4346 pubmed publisher
Duan H, Jing L, Xiang J, Ju C, Wu Z, Liu J, et al. CD146 Associates with Gp130 to Control a Macrophage Pro-inflammatory Program That Regulates the Metabolic Response to Obesity. Adv Sci (Weinh). 2022;9:e2103719 pubmed publisher
Azuma Y, Fujita T, Izawa T, Hirota K, Nishiyama K, Ikegami A, et al. IL-19 Contributes to the Development of Nonalcoholic Steatohepatitis by Altering Lipid Metabolism. Cells. 2021;10: pubmed publisher
Medina Buelvas D, Rodr xed guez Sosa M, Vega L. Characterisation of Macrophage Polarisation in Mice Infected with Ninoa Strain of Trypanosoma cruzi. Pathogens. 2021;10: pubmed publisher
Ji W, Li D, Wei D, Gu A, Yang Y, Peng J. Cytochrome P450 26A1 Modulates the Polarization of Uterine Macrophages During the Peri-Implantation Period. Front Immunol. 2021;12:763067 pubmed publisher
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Oladiran O, Shi X, Fournier S, Zhang J. CX3CR1 But Not CCR2 Expression Is Required for the Development of Autoimmune Peripheral Neuropathy in Mice. Front Immunol. 2021;12:720733 pubmed publisher
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Heimesaat M, Weschka D, Mousavi S, Bereswill S. Treatment with the Probiotic Product Aviguard® Alleviates Inflammatory Responses during Campylobacter jejuni-Induced Acute Enterocolitis in Mice. Int J Mol Sci. 2021;22: pubmed publisher
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Mousavi S, Weschka D, Bereswill S, Heimesaat M. Preclinical Evaluation of Oral Urolithin-A for the Treatment of Acute Campylobacteriosis in Campylobacter jejuni Infected Microbiota-Depleted IL-10-/- Mice. Pathogens. 2020;10: pubmed publisher
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Heimesaat M, Mrazek K, Bereswill S. Murine Fecal Microbiota Transplantation Alleviates Intestinal and Systemic Immune Responses in Campylobacter jejuni Infected Mice Harboring a Human Gut Microbiota. Front Immunol. 2019;10:2272 pubmed publisher
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Zhou Y, Fu C, Kong Y, Pan D, Wang Y, Huang S, et al. Antitumor and immunomodulatory effects of a novel multitarget inhibitor, CS2164, in mouse hepatocellular carcinoma models. Anticancer Drugs. 2019;30:909-916 pubmed publisher
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product information
Product Type :
Antibody
Product Name :
F4/80 Monoclonal Antibody (BM8), Super Bright 780, eBioscience™
Catalog # :
78-4801-82
Quantity :
100 µg
Price :
US 428
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Rat
Reactivity :
Mouse
Applications :
Flow Cytometry: 0.5 µg/test
Species :
Mouse
Clone :
BM8
Isotype :
IgG2a, kappa
Storage :
4° C, store in dark, DO NOT FREEZE!
Description :
F4/80 antigen is a 160 kDa cell surface glycoprotein that is a member of the EGF TM7 family of proteins and shares 68% overall amino acid identity with human EGF module containing mucin like hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 0.5 µg/test
Aliases :
ADGRE1; adhesion G protein-coupled receptor E1; Cell surface glycoprotein F4/80; DD7A5-7; egf-like module containing, mucin-like, hormone receptor-like 1; EGF-like module containing, mucin-like, hormone receptor-like sequence 1; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EGF-TM7; EMR1; EMR1 hormone receptor; F4/80; Gpf480; Ly71; lymphocyte antigen 71; TM7LN3
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA