VRK1 Monoclonal Antibody (1F6)
Invitrogen
catalog: MA1-828
mouse monoclonal (1F6)
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western blot analysis of VRK1 was performed by loading 25 ug of the indicated whole cell lysates and 5ul of the Lane Marker Reducing Sample Buffer (Product # 39000) per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a VRK1 monoclonal antibody (Product # MA1-828) at a dilution of 1:750 overnight at 4C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a goat anti-mouse IgG-HRP secondary antibody (Product # 32430) at a dilution of 1:10,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075).

Immunohistochemistry was performed on normal biopsies of deparaffinized human liver tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval, tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and then probed without (left panel) or with (right panel) a VRK1 monoclonal antibody (Product # MA1-828) at a dilution of 1:200 overnight at 4C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and Streptavidin-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
quantity: 100 ug
price: US 440.00
to the supplier
VRK1 Polyclonal Antibody
Invitrogen
catalog: PA5-28687
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

Immunohistochemical analysis of paraffin-embedded Liver using VRK1 (PA5-28687) antibody at 1:250 dilution.

Western Blot analysis of VRK1 using anti-VRK1 Polyclonal Antibody (PA5-28687), in Hela Cell Lysate (30 ug of cell lysate) and at a dilution of 1:1000.
quantity: 100 uL
price: US 464.00
to the supplier
VRK1 Polyclonal Antibody
Invitrogen
catalog: PA5-80222
domestic rabbit polyclonal
reactivity: human, rat
application: western blot
quantity: 100 ug
price: US 404.00
to the supplier
VRK1 Polyclonal Antibody
Invitrogen
catalog: PA5-81115
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunoprecipitation
quantity: 100 uL
price: US 319.00
to the supplier
VRK1 Polyclonal Antibody
Invitrogen
catalog: PA5-81887
domestic rabbit polyclonal
reactivity: human
application: western blot
quantity: 100 uL
price: US 514.00
to the supplier
VRK1 Polyclonal Antibody
Invitrogen
catalog: PA5-87084
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 481.00
to the supplier
VRK1 Polyclonal Antibody
Invitrogen
catalog: PA5-96785
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 464.00
to the supplier