DYKDDDDK Tag Recombinant Superclonal Antibody (20H18L16, 20H1L23, 8H2L5, 8H8L17)

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

DYKDDDDK Tag Recombinant Superclonal Antibody (20H18L16, 20H1L23, 8H2L5, 8H8L17)

catalog :

740001

quantity :

100 ug

price :

US 463.00

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

20H18L16, 20H1L23, 8H2L5, 8H8L17

application :

western blot, immunocytochemistry, immunoprecipitation

citations: 14

Reference
de la Cruz Herrera C, Tatham M, Siddiqi U, Shire K, Marcon E, Greenblatt J, et al. Changes in SUMO-modified proteins in Epstein-Barr virus infection identifies reciprocal regulation of TRIM24/28/33 complexes and the lytic switch BZLF1. PLoS Pathog. 2023;19:e1011477 pubmed publisher
Linghu C, An B, Shpokayte M, Celiker O, Shmoel N, Zhang R, et al. Recording of cellular physiological histories along optically readable self-assembling protein chains. Nat Biotechnol. 2023;: pubmed publisher
Stanton A, Lagerborg K, Tellez L, Krunnfusz A, King E, Ye S, et al. Systemic administration of novel engineered AAV capsids facilitates enhanced transgene expression in the macaque CNS. Med. 2023;4:31-50.e8 pubmed publisher
Brauer E, Lange T, Keller D, G xf6 rlitz S, Cho S, Keye J, et al. Dissecting the influence of cellular senescence on cell mechanics and extracellular matrix formation in vitro. Aging Cell. 2022;:e13744 pubmed publisher
Groenendyk J, Stoletov K, Paskevicius T, Li W, Dai N, Pujol M, et al. Loss of the fructose transporter SLC2A5 inhibits cancer cell migration. Front Cell Dev Biol. 2022;10:896297 pubmed publisher
Hyun S, Imamura A, Ishida H, Piepenbrink K, Goldblum S, Lillehoj E. The sialidase NEU1 directly interacts with the juxtamembranous segment of the cytoplasmic domain of mucin-1 to inhibit downstream PI3K-Akt signaling. J Biol Chem. 2021;297:101337 pubmed publisher
Liao Y, Lupiani B, Reddy S. Manipulation of Promyelocytic Leukemia Protein Nuclear Bodies by Marek's Disease Virus Encoded US3 Protein Kinase. Microorganisms. 2021;9: pubmed publisher
Holt I, Fuller H, Schindler R, Shirran S, Brand T, Morris G. An interaction of heart disease-associated proteins POPDC1/2 with XIRP1 in transverse tubules and intercalated discs. BMC Mol Cell Biol. 2020;21:88 pubmed publisher
Linghu C, Johnson S, Valdes P, Shemesh O, Park W, Park D, et al. Spatial Multiplexing of Fluorescent Reporters for Imaging Signaling Network Dynamics. Cell. 2020;183:1682-1698.e24 pubmed publisher
Zein Sabatto H, Cole T, Hoang H, Tiwary E, Chang C, Miller M. The type II integral ER membrane protein VAP-B homolog in C. elegans is cleaved to release the N-terminal MSP domain to signal non-cell-autonomously. Dev Biol. 2021;470:10-20 pubmed publisher
Rico A, Wang Z, Olson A, Linville A, Bullard B, Weaver E, et al. The Vaccinia Virus (VACV) B1 and Cellular VRK2 Kinases Promote VACV Replication Factory Formation through Phosphorylation-Dependent Inhibition of VACV B12. J Virol. 2019;93: pubmed publisher
Siddiqi U, Vaidya A, Li X, Marcon E, Tsao S, Greenblatt J, et al. Identification of ARKL1 as a Negative Regulator of Epstein-Barr Virus Reactivation. J Virol. 2019;93: pubmed publisher
Li Y, Duah E, Long N, Persaud A, Vangundy Z, MAGLIERY T, et al. An efficient and quantitative assay for epitope-tagged therapeutic protein development with a capillary western system. Bioanalysis. 2019;11:471-483 pubmed publisher
Patel N, Wang J, Shiozawa K, Jones K, Zhang Y, Prokop J, et al. HDAC2 Regulates Site-Specific Acetylation of MDM2 and Its Ubiquitination Signaling in Tumor Suppression. iScience. 2019;13:43-54 pubmed publisher

product information

Product Type :

Antibody

Product Name :

DYKDDDDK Tag Recombinant Superclonal Antibody (20H18L16, 20H1L23, 8H2L5, 8H8L17)

Catalog # :

740001

Quantity :

100 ug

Price :

US 463.00

Clonality :

Recombinant Superclonal

Purity :

protein A

Host :

Rabbit

Reactivity :

Tag

Applications :

Immunocytochemistry: 1 ug/mL, Immunoprecipitation: 1 ug/mL, Western Blot: 0.5-1 ug/mL

Species :

Tag

Clone :

20H18L16, 20H1L23, 8H2L5, 8H8L17

Isotype :

IgG

Storage :

Store at 4 C short term. For long term storage, store at -20 C, avoiding freeze/thaw cycles.

Description :

DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence that is commonly used as a protein tag to facilitate protein purification, detection, and localization. The tag consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys. It is often fused to the N- or C-terminus of a protein of interest, allowing for easy identification and isolation of the protein using anti-FLAG antibodies. The DYKDDDDK tag is widely used in molecular biology and biochemistry research, and has been employed in various applications, such as protein-protein interaction studies, protein expression analysis, and protein localization studies. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.

Immunogen :

Synthetic peptide (DYKDDDDK)

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1 ug/mL, Immunoprecipitation: 1 ug/mL, Western Blot: 0.5-1 ug/mL

Aliases :

DDDDK; DDDDK epitope tag; ECS epitope tag; ECS tag; FLAG; FLAG Tag