mouse monoclonal (4D11)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
citations: 30

Immunofluorescent analysis of g-Tubulin using anti-g-Tubulin monoclonal antibody (Pierce Product# MA1-850, Clone 4D11) (shown in green) in HeLa (A) and U2-OS cells (B). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 1% Blocker BSA (Pierce Product #37525) for 15 minutes at room temperature. Cells were probed with a mouse monoclonal antibody recognizing g-Tubulin (Pierce Product# MA1-850), at a dilution of 1:50 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goat-anti-mouse secondary antibody (Pierce Product# 35503) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DY-547 phalloidin, nuclei (blue) were stained with Hoechst 33342 dye (Pierce Product# 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.

Western blot analysis of ?-Tubulin was performed by loading 50ug of various whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with a mouse monoclonal antibody recognizing g-Tubulin (Pierce Product # MA1-850, Clone 4D11) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody (Pierce Product # 31430) at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed using Pierce Super Signal West Dura (Pierce Product #34075).
quantity: 100 ug
price: US 412.00
to the supplier
gamma Tubulin Monoclonal Antibody (TU-30)
Invitrogen
catalog: MA1-19421
mouse monoclonal (TU-30)
reactivity: human, mouse, rat, chicken, bovine, pigs
application: western blot, immunocytochemistry, flow cytometry
citations: 9



quantity: 100 ug
price: US 558.00
to the supplier
gamma Tubulin Polyclonal Antibody
Invitrogen
catalog: PA1-28042
domestic rabbit polyclonal
reactivity: Chinese hamsters, human, mouse, rat, chicken
application: western blot, immunocytochemistry
citations: 3
quantity: 100 uL
price: US 538.00
to the supplier
gamma Tubulin Polyclonal Antibody
Invitrogen
catalog: PA5-34815
domestic rabbit polyclonal
reactivity: human, mouse, rat, zebrafish
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
citations: 3
quantity: 100 uL
price: US 538.00
to the supplier
gamma Tubulin Monoclonal Antibody (GT4511)
Invitrogen
catalog: MA5-31482
mouse monoclonal (GT4511)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
citations: 1
quantity: 100 uL
price: US 480.00
to the supplier
mouse monoclonal (4D11)
reactivity: human, mouse
conjugate: Alexa Fluor 555
application: immunocytochemistry
citations: 1
quantity: 50 ug
price: US 425.00
to the supplier
domestic rabbit recombinant (JF0972)
reactivity: Chinese hamsters, human, mouse, rat, dogs, chicken, bovine, Xenopus laevis
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 493.00
to the supplier
gamma Tubulin Polyclonal Antibody
Invitrogen
catalog: PA5-86260
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: immunohistochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 464.00
to the supplier
gamma Tubulin Polyclonal Antibody
Invitrogen
catalog: PA5-87132
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot
quantity: 100 uL
price: US 481.00
to the supplier
mouse monoclonal (4D11)
reactivity: human, mouse
conjugate: AF488
application: immunocytochemistry
quantity: 50 ug
price: US 425.00
to the supplier
mouse monoclonal (4D11)
reactivity: human, mouse
conjugate: AF647
application: immunocytochemistry
quantity: 50 ug
price: US 425.00
to the supplier
TUBG1 Polyclonal Antibody
Invitrogen
catalog: PA5-96533
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA
quantity: 100 uL
price: US 454.00
to the supplier
TUBG1 Polyclonal Antibody
Invitrogen
catalog: PA5-112781
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section
quantity: 100 ug
price: US 430.00
to the supplier
domestic rabbit recombinant (ARC1684)
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 484.00
to the supplier
domestic rabbit recombinant (6T10A1)
reactivity: human, mouse, rat
application: western blot, ELISA, immunoprecipitation
quantity: 100 uL
price: US 470.00
to the supplier
mouse monoclonal (TU-30)
reactivity: human, mouse, rat, chicken, bovine, pigs
conjugate: AF647
application: western blot, immunocytochemistry, flow cytometry
quantity: 100 ug
price: US 470.00
to the supplier
gamma Tubulin Monoclonal Antibody (10-A4)
Invitrogen
catalog: MA5-44601
mouse monoclonal (10-A4)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 470.00
to the supplier
domestic rabbit recombinant (HL1175)
reactivity: human, mouse, rat, dogs, cat
application: western blot, immunocytochemistry
quantity: 100 uL
price: US 444.00
to the supplier
domestic rabbit recombinant (6T10A1)
reactivity: human, mouse, rat
application: western blot, ELISA
quantity: 100 uL
price: US 424.00
to the supplier
gamma Tubulin Polyclonal Antibody
Invitrogen
catalog: PA5-116090
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 464.00
to the supplier
gamma Tubulin Polyclonal Antibody
Invitrogen
catalog: PA5-140996
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 460.00
to the supplier
domestic rabbit recombinant (23GB4805)
reactivity: human, mouse, rat
application: western blot, flow cytometry
quantity: 100 uL
price: US 539.00
to the supplier
domestic rabbit recombinant (23GB4805)
reactivity: human, mouse, rat
application: western blot, flow cytometry
quantity: 20 uL
price: US 199.00
to the supplier