SSEA1 Monoclonal Antibody (eBioMC-480 (MC-480)), Biotin, eBioscience
Invitrogen
catalog: 13-8813-82
mouse monoclonal (eBioMC-480 (MC-480))
reactivity: human, mouse
conjugate: biotin
application: immunocytochemistry, flow cytometry
citations: 27
quantity: 100 µg
price: US 349
to the supplier
SSEA1 Monoclonal Antibody (eBioMC-480 (MC-480)), eBioscience
Invitrogen
catalog: 14-8813-82
mouse monoclonal (eBioMC-480 (MC-480))
reactivity: human, mouse
application: immunohistochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section
citations: 28
quantity: 100 µg
price: US 311
to the supplier
SSEA1 Monoclonal Antibody (eBioMC-480 (MC-480)), Alexa Fluor™ 488, eBioscience
Invitrogen
catalog: 53-8813-42
mouse monoclonal (eBioMC-480 (MC-480))
reactivity: human, mouse
conjugate: AF488
application: flow cytometry
citations: 24
quantity: 100 Tests
price: US 478
to the supplier
SSEA1 Monoclonal Antibody (MC-480)
Invitrogen
catalog: MA1-022
mouse monoclonal (MC-480)
reactivity: human, mouse
application: immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 9

Flow cytometry analysis of SSEA-1 (blue histogram) on H9 human embryonic stem cells. Cells were harvested, fixed with 4% formaldehyde, washed with PBS, and incubated with a SSEA-1 monoclonal antibody (Product # MA1-022) or an IgM isotype control (red histogram) at a 1:100 dilution for 1 hour on ice. For flow analysis, a 30-minute incubation with fluorescein conjugated secondary antibody was performed and 100,000 cells were acquired for each sample.

Flow cytometry analysis of SSEA-1 (blue histogram) on differentiated HEL 11.4 human iPS cells. Cells were harvested, fixed with 4% formaldehyde, washed with PBS, and incubated with a SSEA-1 monoclonal antibody (Product # MA1-022) or an IgM isotype control (red histogram) at a 1:100 dilution for 1 hour on ice. For flow analysis, a 30-minute incubation with fluorescein conjugated secondary antibody was performed and 100,000 cells were acquired for each sample.

Immunofluorescent analysis of SSEA-1 using anti-SSEA-1 monoclonal antibody (Product# MA1-022) shows staining on the cell surface of human differentiated HEL 11.4 iPS cells, indicating loss of pluripotency. SSEA-1 staining (green) and an overlay image of SSEA-1 with DAPI (blue) is shown. HEL 11.4 cells were grown on matrigel coated chamber slides and fixed with formaldehyde prior to staining. Cells were probed with a mouse monoclonal antibody recognizing SSEA-1 (Product# MA1-022) at a dilution of 1:120 over night at 4ºC, washed with PBS and incubated with a fluorescein conjugated secondary antibody at a dilution of 1:100 for 60 minutes at room temperature. Images were taken at 20X magnification.
quantity: 100 µg
price: US 410
to the supplier
SSEA1 Monoclonal Antibody (MC-480)
Invitrogen
catalog: MA1-022X
mouse monoclonal (MC-480)
reactivity: human, mouse
application: immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 9

Immunofluorescent analysis of SSEA-1 using anti-SSEA-1 monoclonal antibody (Product# MA1-022) shows staining on the cell surface of peripheral human embryonic H9 stem cells, indicating partial loss of pluripotency. SSEA-1 staining (green) and an overlay image of SSEA-1 with DAPI (blue) is shown. H9 cells were grown on matrigel coated chamber slides and fixed with formaldehyde prior to staining. Cells were probed with a mouse monoclonal antibody recognizing SSEA-1 (Product# MA1-022) at a dilution of 1:120 over night at 4ºC, washed with PBS and incubated with a fluorescein conjugated secondary antibody at a dilution of 1:100 for 60 minutes at room temperature. Images were taken at 20X magnification.

Flow cytometry analysis of SSEA-1 (blue histogram) on H9 human embryonic stem cells. Cells were harvested, fixed with 4% formaldehyde, washed with PBS, and incubated with a SSEA-1 monoclonal antibody (Product # MA1-022) or an IgM isotype control (red histogram) at a 1:100 dilution for 1 hour on ice. For flow analysis, a 30-minute incubation with fluorescein conjugated secondary antibody was performed and 100,000 cells were acquired for each sample.

Flow cytometry analysis of SSEA-1 (blue histogram) on differentiated HEL 11.4 human iPS cells. Cells were harvested, fixed with 4% formaldehyde, washed with PBS, and incubated with a SSEA-1 monoclonal antibody (Product # MA1-022) or an IgM isotype control (red histogram) at a 1:100 dilution for 1 hour on ice. For flow analysis, a 30-minute incubation with fluorescein conjugated secondary antibody was performed and 100,000 cells were acquired for each sample.
quantity: 20 µL
price: US 185
to the supplier